Others Tth DNA Polymerase solution Tth DNA Polymerase For further processing on its own or in a mixture as part of an IVD method only. 3.6.14.3.10.1 from Thermus species, expressed in E. coli, solution Tth DNA Polymerase custom fill CustomBiotech product. Please contact your local representative. Will be supplied as "Tth DNA Polymerase". Unit of measure is "kU". The enzyme is supplied without reaction buffer. Tth DNA Polymerase, HB8 11485954103 Not Available Reagents, kits en Use Tth DNA Polymerase for: One-step RT-PCR of single copy genes from eucaryotic genomes in the presence of Mn2+ ionsOne-step RT-PCR of a specific transcript or an entire population of transcriptsPCR in the presence of Mg2+ ionsLabeling of PCR products with modified nucleotides en Tth DNA Polymerase is a thermostable DNA Polymerase with intrinsic reverse transcriptase activity for RT-PCR amplification of RNA to a length of at least 1 kb. en Amplify directly from RNA. Benefit from the intrinsic reverse transcriptase activity of Tth Polymerase to directly amplify from RNA in one step. Improve PCR yield. Obtain more PCR product, because the Tth Polymerase is stable during prolonged repetitive high temperature incubations. Enhance specificity of amplification. Avoid loss of specificity due to RNA secondary structure using the higher annealing temperature Tth Polymerase allows compared to other reverse transcriptases. en Appearance: Clear, colorless solutionStorage buffer: Tris/HCl, 10 mmol/l; KCl, 300 mmol/l; EDTA, 0.1 mmol/l; DTT, 1 mmol/l; Triton X-100, 0.1% (v/v); glycerol, 50% (v/v); pH approximately 7.5 at +25°CVolume activity: ≥5 kU/mlUnit definition: One unit Tth DNA Polymerase is defined as the amount of enzyme that incorporates 10 nmol of total deoxyribonucleosidetriphosphates into acid precipitable DNA within 30 minutes at +75°C under standard assay conditions.Unspecific endonucleases (λDNA): Not detectable in up to 20 U after 16 hours incubation at +37°C.Nicking activity (pBR322 DNA): Not detectable in up to 20 U after 16 hours incubation at +37°C.Ribonucleases (MS2 RNA): Not detectable in up to 20 U after 4 hours incubation at +37°C.Function test in PCR (10 ng human genomic DNA, 1.1 kb collagen fragment): Corresponds to specificationFunction test in RT-PCR (10 ng human liver RNA, 630 bp MCAD fragment): Corresponds to specificationAnimal-derived additives: NoneStability: At -15 to -25°C within specification range for 18 months.