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Thermus species, expressed in
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"DisclaimerGroup2": null,
"RegulatoryDisclaimer3": "For further processing on its own or in a mixture as part of an IVD method only.",
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"MaterialNum": "11485954103",
"MaterialDescription": "Tth DNA Polymerase, HB8",
"RegisteredProductName": "Tth DNA Polymerase",
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"ProductCategoryText": "Reagents, kits",
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"PackSizePIM360": "custom fill",
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"OrderInformation": "Will be supplied as \"Tth DNA Polymerase\". Unit of measure is \"kU\".
The enzyme is supplied without reaction buffer."
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"ProductSpec": [
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"ProductSpecVariant": {
"Chapters": [
{
"Language": "en",
"Value": "Use Tth DNA Polymerase for:
- One-step RT-PCR of single copy genes from eucaryotic genomes in the presence of Mn2+ ions
- One-step RT-PCR of a specific transcript or an entire population of transcripts
- PCR in the presence of Mg2+ ions
- Labeling of PCR products with modified nucleotides
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"Country": "XG",
"Code": "Applications",
"Name": "Applications"
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{
"Language": "en",
"Value": "Tth DNA Polymerase is a thermostable DNA Polymerase with intrinsic reverse transcriptase activity for RT-PCR amplification of RNA to a length of at least 1 kb.",
"Country": "XG",
"Code": "Positioning",
"Name": "Positioning"
},
{
"Language": "en",
"Value": "
- Amplify directly from RNA. Benefit from the intrinsic reverse transcriptase activity of Tth Polymerase to directly amplify from RNA in one step.
- Improve PCR yield. Obtain more PCR product, because the Tth Polymerase is stable during prolonged repetitive high temperature incubations.
- Enhance specificity of amplification. Avoid loss of specificity due to RNA secondary structure using the higher annealing temperature Tth Polymerase allows compared to other reverse transcriptases.
",
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"Code": "Benefits",
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"Value": "
Appearance: Clear, colorless solution
Storage buffer: Tris/HCl, 10 mmol/l; KCl, 300 mmol/l; EDTA, 0.1 mmol/l; DTT, 1 mmol/l; Triton X-100, 0.1% (v/v); glycerol, 50% (v/v); pH approximately 7.5 at +25°C
Volume activity: ≥5 kU/ml
Unit definition: One unit Tth DNA Polymerase is defined as the amount of enzyme that incorporates 10 nmol of total deoxyribonucleosidetriphosphates into acid precipitable DNA within 30 minutes at +75°C under standard assay conditions.
Unspecific endonucleases (λDNA): Not detectable in up to 20 U after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 20 U after 16 hours incubation at +37°C.
Ribonucleases (MS2 RNA): Not detectable in up to 20 U after 4 hours incubation at +37°C.
Function test in PCR (10 ng human genomic DNA, 1.1 kb collagen fragment): Corresponds to specification
Function test in RT-PCR (10 ng human liver RNA, 630 bp MCAD fragment): Corresponds to specification
Animal-derived additives: None
Stability: At -15 to -25°C within specification range for 18 months.",
"Country": "XG",
"Code": "Specification",
"Name": "Specification"
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{
"Language": "en",
"Value": "Tth Polymerase is the recombinant version of the thermostable enzyme from the thermophilic eubacterium
Thermus thermophilus species, expressed in
E. coli.
Enzyme activities: Highly processive 5'-3' DNA polymerase; no 3'-5' exonuclease activity; very efficient intrinsic reverse transcriptase (RT) activity in the presence of manganese ions; no RNase H activity
pH optimum: Approximately 9.0 (+25°C)
Temperature optimum for elongation: Approximately +72°C
Temperature optimum for reverse transcription: Approximately +60 to +70°C
Divalent ion requirement for PCR: Mg
2+Divalent ion requirement for RT activity and RT-PCR: Mn
2+Substrates: Incorporates dNTP, dUTP, dITP, various labeled or modified nucleotides (200 μmol/l each is recommended of normal dNTP, increased concentrations of variants)",
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