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One-step RT-PCR of single copy genes from eucaryotic genomes in the presence of Mn2+ ions
One-step RT-PCR of a specific transcript or an entire population of transcripts
PCR in the presence of Mg2+ ions
Labeling of PCR products with modified nucleotides
",
"Country": "XG",
"Code": "Applications",
"Name": "Applications"
},
{
"Language": "en",
"Value": "Tth DNA Polymerase is a thermostable DNA Polymerase with intrinsic reverse transcriptase activity for RT-PCR amplification of RNA to a length of at least 1 kb.",
"Country": "XG",
"Code": "Positioning",
"Name": "Positioning"
},
{
"Language": "en",
"Value": "
Amplify directly from RNA. Benefit from the intrinsic reverse transcriptase activity of Tth Polymerase to directly amplify from RNA in one step.
Improve PCR yield. Obtain more PCR product, because the Tth Polymerase is stable during prolonged repetitive high temperature incubations.
Enhance specificity of amplification. Avoid loss of specificity due to RNA secondary structure using the higher annealing temperature Tth Polymerase allows compared to other reverse transcriptases.
",
"Country": "XG",
"Code": "Benefits",
"Name": "Benefits"
},
{
"Language": "en",
"Value": "Appearance: Clear, colorless solution Storage buffer: Tris/HCl, 10 mmol/l; KCl, 300 mmol/l; EDTA, 0.1 mmol/l; DTT, 1 mmol/l; Triton X-100, 0.1% (v/v); glycerol, 50% (v/v); pH approximately 7.5 at +25°C Volume activity: ≥5 kU/ml Unit definition: One unit Tth DNA Polymerase is defined as the amount of enzyme that incorporates 10 nmol of total deoxyribonucleosidetriphosphates into acid precipitable DNA within 30 minutes at +75°C under standard assay conditions. Unspecific endonucleases (λDNA): Not detectable in up to 20 U after 16 hours incubation at +37°C. Nicking activity (pBR322 DNA): Not detectable in up to 20 U after 16 hours incubation at +37°C. Ribonucleases (MS2 RNA): Not detectable in up to 20 U after 4 hours incubation at +37°C. Function test in PCR (10 ng human genomic DNA, 1.1 kb collagen fragment): Corresponds to specification Function test in RT-PCR (10 ng human liver RNA, 630 bp MCAD fragment): Corresponds to specification Animal-derived additives: None Stability: At -15 to -25°C within specification range for 18 months.",
"Country": "XG",
"Code": "Specification",
"Name": "Specification"
},
{
"Language": "en",
"Value": "Tth Polymerase is the recombinant version of the thermostable enzyme from the thermophilic eubacterium Thermus thermophilus species, expressed in E. coli. Enzyme activities: Highly processive 5'-3' DNA polymerase; no 3'-5' exonuclease activity; very efficient intrinsic reverse transcriptase (RT) activity in the presence of manganese ions; no RNase H activity pH optimum: Approximately 9.0 (+25°C) Temperature optimum for elongation: Approximately +72°C Temperature optimum for reverse transcription: Approximately +60 to +70°C Divalent ion requirement for PCR: Mg2+ Divalent ion requirement for RT activity and RT-PCR: Mn2+ Substrates: Incorporates dNTP, dUTP, dITP, various labeled or modified nucleotides (200 μmol/l each is recommended of normal dNTP, increased concentrations of variants)",
"Country": "XG",
"Code": "Properties",
"Name": "Properties"
}
]
}
}
]
}
Tth DNA Polymerase
from Thermus species, expressed in E. coli, solution
