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The enzyme is supplied without reaction buffer." } ] }, "Product2Products": { "Product2ProductReference": [ ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Language": "en", "Value": "Use Tth DNA Polymerase for:
  • One-step RT-PCR of single copy genes from eucaryotic genomes in the presence of Mn2+ ions
  • One-step RT-PCR of a specific transcript or an entire population of transcripts
  • PCR in the presence of Mg2+ ions
  • Labeling of PCR products with modified nucleotides
", "Country": "XG", "Code": "Applications", "Name": "Applications" }, { "Language": "en", "Value": "Tth DNA Polymerase is a thermostable DNA Polymerase with intrinsic reverse transcriptase activity for RT-PCR amplification of RNA to a length of at least 1 kb.", "Country": "XG", "Code": "Positioning", "Name": "Positioning" }, { "Language": "en", "Value": "
  • Amplify directly from RNA. Benefit from the intrinsic reverse transcriptase activity of Tth Polymerase to directly amplify from RNA in one step.
  • Improve PCR yield. Obtain more PCR product, because the Tth Polymerase is stable during prolonged repetitive high temperature incubations.
  • Enhance specificity of amplification. Avoid loss of specificity due to RNA secondary structure using the higher annealing temperature Tth Polymerase allows compared to other reverse transcriptases.
", "Country": "XG", "Code": "Benefits", "Name": "Benefits" }, { "Language": "en", "Value": "Appearance: Clear, colorless solution
Storage buffer: Tris/HCl, 10 mmol/l; KCl, 300 mmol/l; EDTA, 0.1 mmol/l; DTT, 1 mmol/l; Triton X-100, 0.1% (v/v); glycerol, 50% (v/v); pH approximately 7.5 at +25°C
Volume activity: ≥5 kU/ml
Unit definition: One unit Tth DNA Polymerase is defined as the amount of enzyme that incorporates 10 nmol of total deoxyribonucleosidetriphosphates into acid precipitable DNA within 30 minutes at +75°C under standard assay conditions.
Unspecific endonucleases (λDNA): Not detectable in up to 20 U after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 20 U after 16 hours incubation at +37°C.
Ribonucleases (MS2 RNA): Not detectable in up to 20 U after 4 hours incubation at +37°C.
Function test in PCR (10 ng human genomic DNA, 1.1 kb collagen fragment): Corresponds to specification
Function test in RT-PCR (10 ng human liver RNA, 630 bp MCAD fragment): Corresponds to specification
Animal-derived additives: None
Stability: At -15 to -25°C within specification range for 18 months.", "Country": "XG", "Code": "Specification", "Name": "Specification" }, { "Language": "en", "Value": "Tth Polymerase is the recombinant version of the thermostable enzyme from the thermophilic eubacterium Thermus thermophilus species, expressed in E. coli.
Enzyme activities: Highly processive 5'-3' DNA polymerase; no 3'-5' exonuclease activity; very efficient intrinsic reverse transcriptase (RT) activity in the presence of manganese ions; no RNase H activity
pH optimum: Approximately 9.0 (+25°C)
Temperature optimum for elongation: Approximately +72°C
Temperature optimum for reverse transcription: Approximately +60 to +70°C
Divalent ion requirement for PCR: Mg2+
Divalent ion requirement for RT activity and RT-PCR: Mn2+
Substrates: Incorporates dNTP, dUTP, dITP, various labeled or modified nucleotides (200 μmol/l each is recommended of normal dNTP, increased concentrations of variants)", "Country": "XG", "Code": "Properties", "Name": "Properties" } ] } } ] }

Tth DNA Polymerase

from Thermus species, expressed in E. coli, solution

Tth DNA Polymerase

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Technical documents

for certificates, method sheets (instructions for use), safety data sheets and software downloads
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