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Two-step RT-PCR applications using conventional thermal cyclers or real-time PCR instruments
RT-PCR for detection of viral RNA
TMA and NASBA nucleic acid amplification methods
Synthesis of full-length cDNA up to 14 kb for libraries or cloning
Rapid amplification of cDNA end (RACE)
",
"Country": "XG",
"Code": "Applications",
"Name": "Applications"
},
{
"Language": "en",
"Value": "Transcriptor Reverse Transcriptase is the robust recombinant reverse transcriptase with thermostability up to +60°C, for transcription of RNA fragments up to 14 kb in two-step RT-PCR applications.",
"Country": "XG",
"Code": "Positioning",
"Name": "Positioning"
},
{
"Language": "en",
"Value": "Will be supplied as \"Transcriptor Bulk\". Unit of measure is \"kU\". The enzyme is supplied without reaction buffer.",
"Country": "XG",
"Code": "CB - Order Information",
"Name": "CB - Order Information"
},
{
"Language": "en",
"Value": "Appearance: Clear, colorless solution Storage buffer: Potassium phosphate, 200 mmol/L; DTT, 2 mmol/L; Triton X-100, 0.2% (v/v); glycerol, 50% (v/v), pH approximately 7.2 Volume activity: ≥20 U/μL Specific activity: ≥50 kU/mg protein Unit definition: One unit Transcriptor Reverse Transcriptase is defined as the amount of enzyme which incorporates 1 nmol of [3H]TMP into an acid insoluble product in 10 minutes at +37°C with poly(A)x(dT)15 as substrate. Purity (SDS PAGE): ≥90% Unspecific endonucleases (MWM III DNA): Not detectable in up to 25 U after 16 hours incubation at +37°C. Nicking activity (pBR322 DNA): Not detectable in up to 25 U after 16 hours incubation at +37°C. Ribonucleases (MS2 RNA): Not detectable in up to 40 U after 4 hours incubation at +37°C. Function test in RT-PCR (human skeletal muscle total RNA, 10 kb dystrophin gene fragment): Corresponds to reference Function test in real-time RT-qPCR using the LightCycler® instrument (PBGD gene fragment from RNA standards): Corresponds to reference Animal-derived additives: None Stability: At -15 to -25°C within specification range for 12 months.",
"Country": "XG",
"Code": "Specification",
"Name": "Specification"
},
{
"Language": "en",
"Value": "Transcriptor Reverse Transcriptase offers higher thermostability compared to the native forms of AMV or M-MLV reverse transcriptase, allowing higher temperatures for reverse transcription, achieving high performance with GC-rich RNA fragments and difficult secondary structures. CAS Number: 9068-38-6 Enzyme activities: RNA-dependent DNA polymerase, DNA-dependent DNA polymerase, unwinding activity, RNase H (degrading RNA in RNA:DNA hybrids) Recommended reaction temperature: +42 to +65°C Substrates: Incorporates dNTP, ddNTP, dUPT, various labeled or modified nucleotides Divalent ion requirement: Mg2+",
"Country": "XG",
"Code": "Properties",
"Name": "Properties"
}
]
}
}
]
}
