For further processing only. Others Tetramethylrhodamine-5-dUTP Tetramethylrhodamine-5-dUTP 3.6.13.2.7.1 1 mM solution, lithium salt 11542907103 Tetramethylrhodamine-5-dUTP Tetramethylrhodamine-5-dUTP 07613336133163 Reagents, kits custom fill Not Available CustomBiotech product. Please contact your local representative. Will be supplied as "Tetramethylrhodamine-5-dUTP". Unit of measure is "nmol". Tetramethylrhodamine-5-dUTP is a substitute for dTTP in nick-translation reactions and in the random-primed labeling technique for DNA labeling, as well as in PCR. The nucleotide also serves as a substrate for Terminal Transferase in 3'-end labeling. Tetramethylrhodamine-labeled probes show red fluorescence and are suitable for use in in situ hybridization for direct fluorescence detection. Multiple fluorescence labeling using Fluorescein-12-dUTP (yellow fluorescence) or other dye-labeled deoxynucleotides is possible. en Tetramethylrhodamine-5-dUTP is a substitute for dTTP in nick-translation and in the random-primed labeling reactions. en Tetramethylrhodamine-5-dUTP is used for nonradioactive labeling of DNA. This modified nucleotide is a substrate for: Terminal TransferaseDNA polymerase I (holoenzyme and Klenow fragment)Taq DNA polymeraseReverse transcriptase (e.g., Transcriptor Reverse Transcriptase and other reverse transcriptases) en Appearance: Clear, red solution Tetramethylrhodamine-5-dUTP (1 μmol ≙ 70.0 A551 units, phosphate buffer, 0.1 mol/L, pH 9.0): 1.0-1.1 mmol/L Purity (HPLC): 85.0-100.0 area% Function test (in situ hybridization): Corresponds to specification Stability: At -15 to -25°C within specification range for 12 months. Store dry. Protect from light. en