{ "ProductData": { "RelatedLinks": "", "RegulatoryDisclaimer1": "For further processing only.", "RegulatoryDisclaimer2": "", "StainLocalization": [ "" ], "ProductType": "Others", "DisclaimerGroup2": null, "ProductNameGlobal": "Tetramethylrhodamine-5-dUTP", "DisclaimerGroup1": "Non-IVD", "ControlTissue": [ "" ], "BrandName": "Tetramethylrhodamine-5-dUTP", "LicenseDisclaimers": [ ], "Isotypes": "", "RegulatoryDisclaimer3": "", "ID": "3.6.13.2.7.1", "ProductNameAddition": "1 mM solution, lithium salt", "Species": [ "" ], "Clone": "" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/CB_IMAGE.png", "ImageType": "Image main" }, "Product2Taxomony": { "Product2TaxomonyReferences": [ { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "NodeName": "Labeled Nucleotides", "NodeID": "70-030-00" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "Molecular Raw Materials", "NodeID": "02-0136" }, { "StructureSystemIdentifier": "OWP_Organization", "StructureSystemName": "Organization", "NodeName": "CustomBiotech and LifeScience", "NodeID": "01-01-01-07-00" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "NodeName": "RT-PCR/qRT-PCR", "NodeID": "300-00" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "NodeName": "CustomBiotech", "NodeID": "520" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeName": "Manufacturing", "NodeID": "180-00" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "NodeName": "NOT APPLICABLE", "NodeID": "999" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "11542907103", "MaterialDescription": "Tetramethylrhodamine-5-dUTP", "RegisteredProductName": "Tetramethylrhodamine-5-dUTP", "GTIN": "07613336133163", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "custom fill", "PackSizeDescPIM360": "", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"Tetramethylrhodamine-5-dUTP\". Unit of measure is \"nmol\"." } ] }, "Product2Products": { "Product2ProductReference": [ ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Language": "en", "Value": "Tetramethylrhodamine-5-dUTP is a substitute for dTTP in nick-translation reactions and in the random-primed labeling technique for DNA labeling, as well as in PCR. The nucleotide also serves as a substrate for Terminal Transferase in 3'-end labeling. Tetramethylrhodamine-labeled probes show red fluorescence and are suitable for use in in situ hybridization for direct fluorescence detection. Multiple fluorescence labeling using Fluorescein-12-dUTP (yellow fluorescence) or other dye-labeled deoxynucleotides is possible.", "Name": "Product Description" }, { "Language": "en", "Value": "Will be supplied as \"Tetramethylrhodamine-5-dUTP\". Unit of measure is \"nmol\".", "Name": "CB - Order Information" }, { "Language": "en", "Value": "Tetramethylrhodamine-5-dUTP is a substitute for dTTP in nick-translation and in the random-primed labeling reactions.", "Name": "Positioning" }, { "Language": "en", "Value": "Tetramethylrhodamine-5-dUTP is used for nonradioactive labeling of DNA. This modified nucleotide is a substrate for:
  • Terminal Transferase
  • DNA polymerase I (holoenzyme and Klenow fragment)
  • Taq DNA polymerase
  • Reverse transcriptase (e.g., Transcriptor Reverse Transcriptase and other reverse transcriptases)
", "Name": "Applications" }, { "Language": "en", "Value": "Appearance: Clear, red solution
Tetramethylrhodamine-5-dUTP (1 μmol ≙ 70.0 A551 units, phosphate buffer, 0.1 mol/L, pH 9.0): 1.0-1.1 mmol/L
Purity (HPLC): 85.0-100.0 area%
Function test (in situ hybridization): Corresponds to specification
Stability: At -15 to -25°C within specification range for 12 months. Store dry. Protect from light.", "Name": "Specification" }, { "Language": "en", "Value": "Formula: C37H36N5O18P3Li4
Molecular weight: 959.4 D", "Name": "Properties" } ] } } ] }

Tetramethylrhodamine-5-dUTP

1 mM solution, lithium salt

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