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"Name": "Product Description",
"Value": "Tetramethylrhodamine-5-dUTP is a substitute for dTTP in nick-translation reactions and in the random-primed labeling technique for DNA labeling, as well as in PCR. The nucleotide also serves as a substrate for Terminal Transferase in 3'-end labeling. Tetramethylrhodamine-labeled probes show red fluorescence and are suitable for use in
in situ hybridization for direct fluorescence detection. Multiple fluorescence labeling using Fluorescein-12-dUTP (yellow fluorescence) or other dye-labeled deoxynucleotides is possible.",
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- DNA polymerase I (holoenzyme and Klenow fragment)
- Taq DNA polymerase
- Reverse transcriptase (e.g., Transcriptor Reverse Transcriptase and other reverse transcriptases)
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Appearance: Clear, red solution
Tetramethylrhodamine-5-dUTP (1 μmol ≙ 70.0 A
551 units, phosphate buffer, 0.1 mol/L, pH 9.0): 1.0-1.1 mmol/L
Purity (HPLC): 85.0-100.0 area%
Function test (
in situ hybridization): Corresponds to specification
Stability: At -15 to -25°C within specification range for 12 months. Store dry. Protect from light.",
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Formula: C
37H
36N
5O
18P
3Li
4
Molecular weight: 959.4 D",
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