{ "ProductData": { "RelatedLinks": "", "RegulatoryDisclaimer1": "", "RegulatoryDisclaimer2": "", "StainLocalization": [ "" ], "ProductType": "Others", "DisclaimerGroup2": null, "ProductNameGlobal": "Terminal Transferase recombinant", "DisclaimerGroup1": null, "ControlTissue": [ "" ], "BrandName": "Terminal Transferase, recombinant", "LicenseDisclaimers": [ ], "Isotypes": "", "RegulatoryDisclaimer3": "For customers in the European Economic Area: Contains SVHC: octyl/nonylphenol ethoxylates. For further processing on its own or in a mixture as part of an IVD method and under controlled conditions only – acc. to Art. 56 (3) and 3 no. 23 REACH Regulation.", "ID": "3.6.14.3.7.2", "ProductNameAddition": "from calf thymus, expressed in E. coli, solution", "Species": [ "" ], "Clone": "" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/CB_IMAGE.png", "ImageType": "Image main" }, "Product2Taxomony": { "Product2TaxomonyReferences": [ { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "NodeName": "Transferases", "NodeID": "70-056-00" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "Molecular Raw Materials", "NodeID": "02-0136" }, { "StructureSystemIdentifier": "OWP_Organization", "StructureSystemName": "Organization", "NodeName": "CustomBiotech and LifeScience", "NodeID": "01-01-01-07-00" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "Enzymes", "NodeID": "02-0014" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "NodeName": "CustomBiotech", "NodeID": "520" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeName": "Manufacturing", "NodeID": "180-00" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "NodeName": "NOT APPLICABLE", "NodeID": "999-00" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "NodeName": "NOT APPLICABLE", "NodeID": "999" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "03289869103", "MaterialDescription": "Terminale Transferase, recombi", "RegisteredProductName": "Terminal Transferase, recombinant", "GTIN": "07613336133156", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "custom fill", "PackSizeDescPIM360": "", "MaterialAnnotation": "Custom Biotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"Terminale Transferase, recombi\". Unit of measure is \"kU\"." } ] }, "Product2Products": { "Product2ProductReference": [ ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Language": "en", "Value": "Will be supplied as \"Terminale Transferase, recombi\". Unit of measure is \"kU\".", "Name": "CB - Order Information" }, { "Language": "en", "Value": "Terminal Transferase catalyzes the template independent addition of deoxy- and dideoxynucleoside triphosphates to the 3'-OH ends of double- and single-stranded DNA fragments and oligonucleotides. Terminal Transferase incorporates digoxigenin-, biotin-, and fluorochrome-labeled deoxy- and dideoxynucleoside triphosphates, as well as radioactively labeled deoxy- and dideoxynucleoside triphosphates. The supplied 5x concentrated reaction buffer facilitates optimal tailing of all types of double-stranded DNA ends: blunt ended, with 3' overhang, or with 5' overhang.", "Name": "Product Description" }, { "Language": "en", "Value": "Terminal Transferase catalyzes the addition of deoxy- and dideoxynucleoside triphosphates to the 3'-OH ends of double- and single-stranded DNA fragments and oligonucleotides.", "Name": "Positioning" }, { "Language": "en", "Value": "Use Terminal Transferase to add homopolymer tails to DNA fragments in cloning experiments, such as addition of overhanging ends onto cDNAs for easier cloning and labeling of 3' ends of double- and single-stranded DNA (e.g., oligonucleotides) with radioactive labeled nucleotides or nucleotides labeled with haptens, e.g., digoxigenin or biotin.", "Name": "Applications" }, { "Language": "en", "Value": "The enzyme is shipped without reaction buffer. Please inquire to obtain an optimized buffer system.", "Name": "Background Information" }, { "Language": "en", "Value": "Appearance: Clear, colorless solution
Storage buffer: Potassium phosphate, 60 mmol/L; KCl, 150 mmol/L; 2-mercaptoethanol, 1 mmol/L; Triton X-100, 0.5% (v/v); glycerol, 50% (v/v); pH approximately 7.2 at +4°C
Volume activity (Co2+): ≥400x103 U/mL
Specific activity (Co2+): ≥200x103 U/mg
Unit definition: One unit is the enzyme activity that leads to an incorporation of 1 nmol dTMP into acid insoluble products within 30 minutes at +37°C under assay conditions (cacodylate, 200 mmol/L; Co2+, 1 mmol/L) using d(pT)6 as primer.
Unspecific endonucleases (MWM II DNA): Not detectable in up to 400 U after 4 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 400 U after 4 hours incubation at +37°C.
Function test (tailing reaction on a 30-mer oligonucleotide): Corresponds to specification
Stability: At -15 to -25°C within specification range for 24 months.", "Name": "Specification" }, { "Language": "en", "Value": "The enzyme catalyzes a template-independent addition of dNTPs or of a single ddNTP to the 3'-OH ends of double- or single-stranded DNA.
It accepts radioactively labeled nucleotides and nucleotides labeled with haptens, such as digoxigenin or biotin.
For activity, the enzyme requires the presence of divalent metal ions, preferably Co2+.
CAS Number: 9027-67-2", "Name": "Properties" } ] } } ] }

Terminal Transferase, recombinant

from calf thymus, expressed in E. coli, solution

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