{ "ProductData": { "RelatedLinks": "", "RegulatoryDisclaimer1": "", "RegulatoryDisclaimer2": "", "StainLocalization": [ "" ], "DisclaimerGroup2": null, "ProductNameGlobal": "Taq DNA Polymerase GMP Grade 5 U uL", "DisclaimerGroup1": null, "ControlTissue": [ "" ], "BrandName": "Taq DNA Polymerase, GMP Grade, 5 U/μl", "LicenseDisclaimers": [ ], "Isotypes": "", "RegulatoryDisclaimer3": "For customers in the European Economic Area: Contains SVHC: octyl/nonylphenol ethoxylates. For further processing on its own or in a mixture as part of an IVD method and under controlled conditions only – acc. to Art. 56 (3) and 3 no. 23 REACH Regulation.", "ID": "3.6.17.2.1.1", "ProductNameAddition": "from Thermus aquaticus BM, expressed in E. coli, solution", "Species": [ "" ], "Clone": "" }, "ProductSpec": { "ProductSpecVariant": { "Chapters": [ { "Language": "English", "Value": "Taq DNA Polymerase is the robust standard enzyme for the amplification of DNA fragments up to 3 kb in PCR.", "Name": "Positioning" }, { "Language": "English", "Value": "

• Obtain consistent results.
  Rely on the robust reaction performance and the lot-to-lot consistency of this product.
• Stay ahead of regulatory requirements.
  High quality manufacturing, quality control and documentation according to GMP (Good Manufacturing Practice) regulations.

", "Name": "Benefits" }, { "Language": "English", "Value": "Use Taq DNA Polymerase, GMP Grade, 5 U/μl, for:

• Routine PCR and RT-PCR applications
• Amplification of DNA fragments up to 3 kb from various sources of DNA
• Labeling of DNA with modified nucleotides (e.g., DIG-dUTP, biotin-dUTP, fluorescein-dUTP)
• Combination with dUTP and Uracil-DNA Glycosylase for prevention of carryover contamination between PCR reactions
• Manufacture of amplification mixtures for applications with regulatory requirements (e.g.,in vitro diagnostics, quality control)

", "Name": "Applications" }, { "Language": "English", "Value": "Appearance: Clear, colorless solution
Storage buffer: Tris/HCl, 20 mmol/L; KCl, 100 mmol/L; DTT, 1 mmol/L; EDTA, 0.1 mmol/L; Nonidet P40, 0.5% (v/v); Tween 20, 0.5% (v/v); glycerol, 50% (v/v); pH approximately 8.0 at +4°C.
Volume activity: ≥5 U/μL
Specific activity (Protein: A₂₈₀): ≥130,000 U/mg
Unit definition: One unit Taq DNA Polymerase is defined as the amount of enzyme that incorporates 10 nmol of total deoxyribonucleosidetriphosphates into acid precipitable DNA within 30 minutes at +75°C under standard assay conditions.
Purity (SDS PAGE): ≥98%
Unspecific endonucleases (λDNA): Not detectable in up to 30 U after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 30 U after 16 hours incubation at +37°C.
Ribonucleases (MS2 RNA): Not detectable in up to 10 U after 1 hour incubation at +37°C.
Function test in PCR (10 pg λDNA, 0.5 kb fragment): Corresponds to reference
Function test in qPCR using LightCycler^®
(human genomic DNA, β-globin gene): Corresponds to reference
(plasmid DNA, β-globin gene): Corresponds to reference
Bioburden: ≤50 CFU/mL
Animal-derived additives: None
Stability: At -15 to -25°C within specification range for 24 months.", "Name": "CB - Specification" }, { "Language": "English", "Value": "03707628103: Will be supplied as \"Taq DNA Polymerase Ind. GMP Grade, 5 kU\". Unit of measure is \"piece\".
03161455103: Will be supplied as \"Taq DNA Polym GMP Grade 50ku\". Unit of measure is \"piece\".
The enzyme is supplied without reaction buffer.", "Name": "CB - Order Information" }, { "Language": "English", "Value": "Manufactured under GMP (Good Manufacturing Practice) regulations.", "Name": "Quality Control" }, { "Language": "English", "Value": "Taq DNA Polymerase is the recombinant full-length version of the thermostable enzyme from the eubacterium Thermus aquaticus BM, expressed in E. coli.
Enzyme acivities: Highly processive 5'-3' DNA polymerase; double-strand specific 5'-3' exonuclease; no 3'-5' exonuclease activity
pH optimum: Approximately 9.0 (+20°C)
Temperature optimum: Approximately +75°C
Half life at +95°C: Approximately 40 minutes
Substrates: Incorporates dNTP, dUPT, dITP, various labeled or modified nucleotides (200 μmol/L each is recommended of normal dNTP, increased concentrations of variants)
Divalent ion requirement: Mg²⁺ (1.5 mmol/L standard concentration)", "Name": "CB - Properties" }, { "Language": "English", "Value": "Appearance: Clear, colorless solution
Storage buffer: Tris/HCl, 20 mmol/L; KCl, 100 mmol/L; DTT, 1 mmol/L; EDTA, 0.1 mmol/L; Nonidet P40, 0.5% (v/v); Tween 20, 0.5% (v/v); glycerol, 50% (v/v); pH approximately 8.0 at +4°C.
Volume activity: ≥5 U/μL
Specific activity (Protein: A₂₈₀): ≥130,000 U/mg
Unit definition: One unit Taq DNA Polymerase is defined as the amount of enzyme that incorporates 10 nmol of total deoxyribonucleosidetriphosphates into acid precipitable DNA within 30 minutes at +75°C under standard assay conditions.
Purity (SDS PAGE): ≥98%
Unspecific endonucleases (λDNA): Not detectable in up to 30 U after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 30 U after 16 hours incubation at +37°C.
Ribonucleases (MS2 RNA): Not detectable in up to 10 U after 1 hour incubation at +37°C.
Function test in PCR (10 pg λDNA, 0.5 kb fragment): Corresponds to reference
Function test in qPCR using LightCycler^®
(human genomic DNA, β-globin gene): Corresponds to reference
(plasmid DNA, β-globin gene): Corresponds to reference
Bioburden: ≤50 CFU/mL
Animal-derived additives: None
Stability: At -15 to -25°C within specification range for 24 months.", "Name": "Specification" }, { "Language": "English", "Value": "Taq DNA Polymerase is the recombinant full-length version of the thermostable enzyme from the eubacterium Thermus aquaticus BM, expressed in E. coli.
Enzyme acivities: Highly processive 5'-3' DNA polymerase; double-strand specific 5'-3' exonuclease; no 3'-5' exonuclease activity
pH optimum: Approximately 9.0 (+20°C)
Temperature optimum: Approximately +75°C
Half life at +95°C: Approximately 40 minutes
Substrates: Incorporates dNTP, dUPT, dITP, various labeled or modified nucleotides (200 μmol/L each is recommended of normal dNTP, increased concentrations of variants)
Divalent ion requirement: Mg²⁺ (1.5 mmol/L standard concentration)", "Name": "Properties" } ] } }, "Product2Taxomony": { "Product2TaxomonyReferences": [ { "StructureSystemName": "OWP Product Solutions", "NodeName": "CustomBiotech", "StructureSystemID": null, "NodeID": "520" }, { "StructureSystemName": "OWP Family", "NodeName": "Taq", "StructureSystemID": null, "NodeID": "530" }, { "StructureSystemName": "OWP Product Types", "NodeName": "Raw Materials and Reagents->DNA Polymerases", "StructureSystemID": null, "NodeID": "70-042-00" }, { "StructureSystemName": "Product Grouping", "NodeName": "CustomBiotech->Molecular Raw Materials", "StructureSystemID": null, "NodeID": "02-0136" }, { "StructureSystemName": "OWP Organization", "NodeName": "Roche Diagnostics Solution (RDS)->Customer Areas->Core lab->CustomBiotech and LifeScience", "StructureSystemID": null, "NodeID": "01-01-01-07-00" }, { "StructureSystemName": "Product Grouping", "NodeName": "CustomBiotech->Enzymes", "StructureSystemID": null, "NodeID": "02-0014" }, { "StructureSystemName": "OWP Techniques", "NodeName": "PCR/qPCR", "StructureSystemID": null, "NodeID": "210-00" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "03707628103", "MaterialDescription": "Taq DNA Polymerase GMP Grade, 5 kU", "RegisteredProductName": "", "GTIN": "07613336133149", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "5 kU", "PackSizeDescPIM360": "5 U/μL", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"Taq DNA Polymerase Ind. GMP Grade, 5 kU\". Unit of measure is \"piece\"." }, { "MaterialNum": "03161455103", "MaterialDescription": "Taq DNA Polym GMP Grade 50ku", "RegisteredProductName": "", "GTIN": "", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "50 kU", "PackSizeDescPIM360": "5 U/μL", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"Taq DNA Polym GMP Grade 50ku\". Unit of measure is \"piece\".
The enzyme is supplied without reaction buffer." } ] }, "Product2Products": { "Product2ProductReference": [ ] } }