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{
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"ProductNameGlobal": "Taq DNA Polymerase GMP Grade 5 U uL",
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"BrandName": "Taq DNA Polymerase, GMP Grade, 5 U/μl",
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"RegulatoryDisclaimer3": "For customers in the European Economic Area: Contains SVHC: octyl/nonylphenol ethoxylates. For further processing on its own or in a mixture as part of an IVD method and under controlled conditions only – acc. to Art. 56 (3) and 3 no. 23 REACH Regulation.",
"ID": "3.6.17.2.1.1",
"ProductNameAddition": "from Thermus aquaticus BM, expressed in E. coli, solution",
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"MaterialDescription": "Taq DNA Polymerase GMP Grade, 5 kU",
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"GTIN": "07613336133149",
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"PackSizePIM360": "5 kU",
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"OrderInformation": "Will be supplied as \"Taq DNA Polymerase Ind. GMP Grade, 5 kU\". Unit of measure is \"piece\"."
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"MaterialDescription": "Taq DNA Polym GMP Grade 50ku",
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"Language": "en",
"Value": "Taq DNA Polymerase is the robust standard enzyme for the amplification of DNA fragments up to 3 kb in PCR.",
"Name": "Positioning"
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"Value": "
Obtain consistent results. Rely on the robust reaction performance and the lot-to-lot consistency of this product.
Stay ahead of regulatory requirements. High quality manufacturing, quality control and documentation according to GMP (Good Manufacturing Practice) regulations.
Amplification of DNA fragments up to 3 kb from various sources of DNA
Labeling of DNA with modified nucleotides (e.g., DIG-dUTP, biotin-dUTP, fluorescein-dUTP)
Combination with dUTP and Uracil-DNA Glycosylase for prevention of carryover contamination between PCR reactions
Manufacture of amplification mixtures for applications with regulatory requirements (e.g.,invitro diagnostics, quality control)
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"Language": "en",
"Value": "03707628103: Will be supplied as \"Taq DNA Polymerase Ind. GMP Grade, 5 kU\". Unit of measure is \"piece\". 03161455103: Will be supplied as \"Taq DNA Polym GMP Grade 50ku\". Unit of measure is \"piece\". The enzyme is supplied without reaction buffer.",
"Name": "CB - Order Information"
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"Language": "en",
"Value": "Manufactured under GMP (Good Manufacturing Practice) regulations.",
"Name": "Quality Control"
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"Language": "en",
"Value": "Appearance: Clear, colorless solution Storage buffer: Tris/HCl, 20 mmol/L; KCl, 100 mmol/L; DTT, 1 mmol/L; EDTA, 0.1 mmol/L; Nonidet P40, 0.5% (v/v); Tween 20, 0.5% (v/v); glycerol, 50% (v/v); pH approximately 8.0 at +4°C. Volume activity: ≥5 U/μL Specific activity (Protein: A280): ≥130,000 U/mg Unit definition: One unit Taq DNA Polymerase is defined as the amount of enzyme that incorporates 10 nmol of total deoxyribonucleosidetriphosphates into acid precipitable DNA within 30 minutes at +75°C under standard assay conditions. Purity (SDS PAGE): ≥98% Unspecific endonucleases (λDNA): Not detectable in up to 30 U after 16 hours incubation at +37°C. Nicking activity (pBR322 DNA): Not detectable in up to 30 U after 16 hours incubation at +37°C. Ribonucleases (MS2 RNA): Not detectable in up to 10 U after 1 hour incubation at +37°C. Function test in PCR (10 pg λDNA, 0.5 kb fragment): Corresponds to reference Function test in qPCR using LightCycler® (human genomic DNA, β-globin gene): Corresponds to reference (plasmid DNA, β-globin gene): Corresponds to reference Bioburden: ≤50 CFU/mL Animal-derived additives: None Stability: At -15 to -25°C within specification range for 24 months.",
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"Language": "en",
"Value": "Taq DNA Polymerase is the recombinant full-length version of the thermostable enzyme from the eubacterium Thermus aquaticus BM, expressed in E. coli. Enzyme acivities: Highly processive 5'-3' DNA polymerase; double-strand specific 5'-3' exonuclease; no 3'-5' exonuclease activity pH optimum: Approximately 9.0 (+20°C) Temperature optimum: Approximately +75°C Half life at +95°C: Approximately 40 minutes Substrates: Incorporates dNTP, dUPT, dITP, various labeled or modified nucleotides (200 μmol/L each is recommended of normal dNTP, increased concentrations of variants) Divalent ion requirement: Mg2+ (1.5 mmol/L standard concentration)",
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Taq DNA Polymerase, GMP Grade, 5 U/μl
from Thermus aquaticus BM, expressed in E. coli, solution