{ "ProductData": { "RelatedLinks": "", "RegulatoryDisclaimer1": "", "RegulatoryDisclaimer2": "", "StainLocalization": [ "" ], "ProductType": "Others", "DisclaimerGroup2": null, "ProductNameGlobal": "Taq DNA Polymerase 50 U uL", "DisclaimerGroup1": null, "ControlTissue": [ "" ], "BrandName": "Taq DNA Polymerase, 50 U/μl", "LicenseDisclaimers": [ ], "Isotypes": "", "RegulatoryDisclaimer3": "For customers in the European Economic Area: Contains SVHC: octyl/nonylphenol ethoxylates. For further processing on its own or in a mixture as part of an IVD method and under controlled conditions only – acc. to Art. 56 (3) and 3 no. 23 REACH Regulation.", "ID": "3.6.17.2.1.8", "ProductNameAddition": "from Thermus aquaticus BM, expressed in E. coli, glycerol-free solution", "Species": [ "" ], "Clone": "" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/CB_IMAGE.png", "ImageType": "Image main" }, "Product2Taxomony": { "Product2TaxomonyReferences": [ { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "NodeName": "Taq", "NodeID": "530" }, { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "NodeName": "DNA Polymerases", "NodeID": "70-042-00" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "Molecular Raw Materials", "NodeID": "02-0136" }, { "StructureSystemIdentifier": "OWP_Organization", "StructureSystemName": "Organization", "NodeName": "CustomBiotech and LifeScience", "NodeID": "01-01-01-07-00" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "Enzymes", "NodeID": "02-0014" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "NodeName": "PCR/qPCR", "NodeID": "210-00" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "NodeName": "CustomBiotech", "NodeID": "520" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeName": "Manufacturing", "NodeID": "180-00" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "04827007103", "MaterialDescription": "Taq DNA Pol., Glycerol-free", "RegisteredProductName": "Taq DNA Polymerase, 50 U/uL", "GTIN": "", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "custom fill", "PackSizeDescPIM360": "", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"Taq DNA Pol., Glycerol-free\". Unit of measure is \"kU\".
The enzyme is supplied without reaction buffer." } ] }, "Product2Products": { "Product2ProductReference": [ { "ProductID": "3.6.14.3.1.1", "BrandName": "Taq DNA Polymerase, 5 U/μl", "ProductNameAddition": "from Thermus aquaticus BM, expressed in E. coli, solution", "ReferenceType": "Reagent", "Classification": [ { "IdentifierofStructureSystem": "OWP_Family", "NameofStructureSystem": "Product Families", "StructureNodeID": "530", "StructureGroupPath": "Taq", "StructureGroupName": "Taq" }, { "IdentifierofStructureSystem": "OWP_Product_Types", "NameofStructureSystem": "Product Types", "StructureNodeID": "70-042-00", "StructureGroupPath": "Raw Materials->DNA Polymerases", "StructureGroupName": "DNA Polymerases" }, { "IdentifierofStructureSystem": "Product_Grouping", "NameofStructureSystem": "Product Grouping", "StructureNodeID": "02-0136", "StructureGroupPath": "CustomBiotech->Molecular Raw Materials", "StructureGroupName": "Molecular Raw Materials" }, { "IdentifierofStructureSystem": "OWP_Organization", "NameofStructureSystem": "Organization", "StructureNodeID": "01-01-01-07-00", "StructureGroupPath": "Roche Diagnostics Solution (RDS)->Customer Areas->Core lab->CustomBiotech and LifeScience", "StructureGroupName": "CustomBiotech and LifeScience" }, { "IdentifierofStructureSystem": "Product_Grouping", "NameofStructureSystem": "Product Grouping", "StructureNodeID": "02-0014", "StructureGroupPath": "CustomBiotech->Enzymes", "StructureGroupName": "Enzymes" }, { "IdentifierofStructureSystem": "OWP_Techniques", "NameofStructureSystem": "Techniques", "StructureNodeID": "210-00", "StructureGroupPath": "PCR/qPCR", "StructureGroupName": "PCR/qPCR" }, { "IdentifierofStructureSystem": "Product_Solutions", "NameofStructureSystem": "Product Solutions", "StructureNodeID": "520", "StructureGroupPath": "CustomBiotech", "StructureGroupName": "CustomBiotech" }, { "IdentifierofStructureSystem": "Lab_Type", "NameofStructureSystem": "Lab Types", "StructureNodeID": "180-00", "StructureGroupPath": "Manufacturing", "StructureGroupName": "Manufacturing" }, { "IdentifierofStructureSystem": "Applications", "NameofStructureSystem": "Applications", "StructureNodeID": "99-00-00", "StructureGroupPath": "NOT APPLICABLE", "StructureGroupName": "NOT APPLICABLE" }, { "IdentifierofStructureSystem": "Disease_Areas", "NameofStructureSystem": "Disease Areas", "StructureNodeID": "99-00-00", "StructureGroupPath": "NOT APPLICABLE", "StructureGroupName": "NOT APPLICABLE" }, { "IdentifierofStructureSystem": "Pathogens", "NameofStructureSystem": "Pathogens", "StructureNodeID": "99-00-00", "StructureGroupPath": "NOT APPLICABLE", "StructureGroupName": "NOT APPLICABLE" }, { "IdentifierofStructureSystem": "Health_Topics", "NameofStructureSystem": "Health Topics", "StructureNodeID": "99-00-00", "StructureGroupPath": "NOT APPLICABLE", "StructureGroupName": "NOT APPLICABLE" } ] } ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Language": "en", "Value": "Use Taq DNA Polymerase, 50 U/μl, especially for:
  • Setup of PCR master mixtures, when highly concentrated components are required
  • Preparation of dried amplification mixtures for more convenience and increased stability at ambient temperature

For further applications see Taq DNA Polymerase, GMP Grade, 5 U/μl", "Name": "Applications" }, { "Language": "en", "Value": "Will be supplied as \"Taq DNA Pol., Glycerol-free\". Unit of measure is \"kU\".
The enzyme is supplied without reaction buffer.", "Name": "CB - Order Information" }, { "Language": "en", "Value": "High concentrated, glycerol-free solution, ideal for preparation of dried-down amplification mixtures.", "Name": "Product Description" }, { "Language": "en", "Value": "Prepare dried amplification mixtures. Use this formulation for manufacture of dried-down reagents with high stability and convenience.", "Name": "Benefits" }, { "Language": "en", "Value": "Taq DNA Polymerase is the robust standard enzyme for the amplification of DNA fragments up to 3 kb in PCR, lyo ready formulation for preparation of dried amplification mixes.", "Name": "Positioning" }, { "Language": "en", "Value": "See Taq DNA Polymerase, GMP Grade, 5 U/μl", "Name": "Properties" }, { "Language": "en", "Value": "Appearance: Clear, colorless solution
Storage buffer: Tris/HCl, 20 mmol/L; KCl, 100 mmol/L; DTT, 1 mmol/L; EDTA, 0.1 mmol/L; Nonidet P40, 0.5% (v/v); Tween 20, 0.5% (v/v); pH approximately 8.0 at +4°C
Glycerol content: ≤0.1% (v/v)
Volume activity: 55±5 U/μL
Unit definition: One unit Taq DNA Polymerase is defined as the amount of enzyme that incorporates 10 nmol of total deoxyribonucleosidetriphosphates into acid precipitable DNA within 30 minutes at +75°C under standard assay conditions.
Unspecific endonucleases (λDNA): Not detectable in up to 30 U after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 30 U after 16 hours incubation at +37°C.
Exonucleases (3H-DNA): Not detectable in up to 30 U after 4 hours incubation at +65°C.
Function test in qPCR using LightCycler® 480 System (≥3 ng of human genomic DNA, 339 bp tPA fragment): Corresponds to reference
Animal-derived additives: None
Stability: At -15 to -25°C within specification range for 24 months.", "Name": "Specification" } ] } } ] }

Taq DNA Polymerase, 50 U/μl

from Thermus aquaticus BM, expressed in E. coli, glycerol-free solution

logo

Overview

Ordering

Technical documents

error errorMessage
Sorry, we couldn't find the content you are looking for
Please try again later