You appear to be using incognito/private browsing mode or an ad blocker, which may adversely affect your experience on the site. Please disable any ad blockers and view the site in non-private mode.
Setup of PCR master mixtures, when highly concentrated components are required
Preparation of dried amplification mixtures for more convenience and increased stability at ambient temperature
For further applications see Taq DNA Polymerase, GMP Grade, 5 U/μl",
"Country": "XG",
"Code": "Applications",
"Name": "Applications"
},
{
"Language": "en",
"Value": "Will be supplied as \"Taq DNA Pol., Glycerol-free\". Unit of measure is \"kU\". The enzyme is supplied without reaction buffer.",
"Country": "XG",
"Code": "CB - Order Information",
"Name": "CB - Order Information"
},
{
"Language": "en",
"Value": "High concentrated, glycerol-free solution, ideal for preparation of dried-down amplification mixtures.",
"Country": "XG",
"Code": "Product Description",
"Name": "Product Description"
},
{
"Language": "en",
"Value": "Prepare dried amplification mixtures. Use this formulation for manufacture of dried-down reagents with high stability and convenience.",
"Country": "XG",
"Code": "Benefits",
"Name": "Benefits"
},
{
"Language": "en",
"Value": "Taq DNA Polymerase is the robust standard enzyme for the amplification of DNA fragments up to 3 kb in PCR, lyo ready formulation for preparation of dried amplification mixes.",
"Country": "XG",
"Code": "Positioning",
"Name": "Positioning"
},
{
"Language": "en",
"Value": "See Taq DNA Polymerase, GMP Grade, 5 U/μl",
"Country": "XG",
"Code": "Properties",
"Name": "Properties"
},
{
"Language": "en",
"Value": "Appearance: Clear, colorless solution Storage buffer: Tris/HCl, 20 mmol/L; KCl, 100 mmol/L; DTT, 1 mmol/L; EDTA, 0.1 mmol/L; Nonidet P40, 0.5% (v/v); Tween 20, 0.5% (v/v); pH approximately 8.0 at +4°C Glycerol content: ≤0.1% (v/v) Volume activity: 55±5 U/μL Unit definition: One unit Taq DNA Polymerase is defined as the amount of enzyme that incorporates 10 nmol of total deoxyribonucleosidetriphosphates into acid precipitable DNA within 30 minutes at +75°C under standard assay conditions. Unspecific endonucleases (λDNA): Not detectable in up to 30 U after 16 hours incubation at +37°C. Nicking activity (pBR322 DNA): Not detectable in up to 30 U after 16 hours incubation at +37°C. Exonucleases (3H-DNA): Not detectable in up to 30 U after 4 hours incubation at +65°C. Function test in qPCR using LightCycler® 480 System (≥3 ng of human genomic DNA, 339 bp tPA fragment): Corresponds to reference Animal-derived additives: None Stability: At -15 to -25°C within specification range for 24 months.",
"Country": "XG",
"Code": "Specification",
"Name": "Specification"
}
]
}
}
]
}
Taq DNA Polymerase, 50 U/μl
from Thermus aquaticus BM, expressed in E. coli, glycerol-free solution
