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The enzyme is supplied without reaction buffer." } ] }, "Product2Products": { "Product2ProductReference": [ { "ProductID": "3.6.14.3.1.1", "BrandName": "Taq DNA Polymerase, 5 U/μl", "ProductNameAddition": "from Thermus aquaticus BM, expressed in E. coli, solution", "ReferenceType": "Reagent", "Classification": [ { "IdentifierofStructureSystem": "OWP_Family", "NameofStructureSystem": "Product Families", "StructureNodeID": "530", "StructureGroupPath": "Taq", "StructureGroupName": "Taq", "StructureNodeStatus": "valid" }, { "IdentifierofStructureSystem": "OWP_Product_Types", "NameofStructureSystem": "Product Types", "StructureNodeID": "70-042-00", "StructureGroupPath": "Raw Materials->DNA Polymerases", "StructureGroupName": "DNA Polymerases", "StructureNodeStatus": "valid" }, { "IdentifierofStructureSystem": "Product_Grouping", "NameofStructureSystem": "Product Grouping", "StructureNodeID": "02-0136", "StructureGroupPath": "CustomBiotech->Molecular Raw Materials", "StructureGroupName": "Molecular Raw Materials", "StructureNodeStatus": "valid" }, { "IdentifierofStructureSystem": "OWP_Organization", "NameofStructureSystem": "Organization", "StructureNodeID": "01-01-01-07-00", "StructureGroupPath": "Roche Diagnostics Solution (RDS)->Customer Areas->Core lab->CustomBiotech and LifeScience", "StructureGroupName": "CustomBiotech and LifeScience", "StructureNodeStatus": "valid" }, { "IdentifierofStructureSystem": "Product_Grouping", "NameofStructureSystem": "Product Grouping", "StructureNodeID": "02-0014", "StructureGroupPath": "CustomBiotech->Enzymes", "StructureGroupName": "Enzymes", "StructureNodeStatus": "valid" }, { "IdentifierofStructureSystem": "OWP_Techniques", "NameofStructureSystem": "Techniques", "StructureNodeID": "210-00", "StructureGroupPath": "PCR/qPCR", "StructureGroupName": "PCR/qPCR", "StructureNodeStatus": "valid" }, { "IdentifierofStructureSystem": "Product_Solutions", "NameofStructureSystem": "Product Solutions", "StructureNodeID": "520", "StructureGroupPath": "CustomBiotech", "StructureGroupName": "CustomBiotech", "StructureNodeStatus": "valid" }, { "IdentifierofStructureSystem": "Lab_Type", "NameofStructureSystem": "Lab Types", "StructureNodeID": "180-00", "StructureGroupPath": "", "StructureGroupName": "", "StructureNodeStatus": "invalid" }, { "IdentifierofStructureSystem": "Applications", "NameofStructureSystem": "Applications", "StructureNodeID": "99-00-00", "StructureGroupPath": "", "StructureGroupName": "", "StructureNodeStatus": "invalid" }, { "IdentifierofStructureSystem": "Disease_Areas", "NameofStructureSystem": "Disease Areas", "StructureNodeID": "99-00-00", "StructureGroupPath": "", "StructureGroupName": "", "StructureNodeStatus": "invalid" }, { "IdentifierofStructureSystem": "Pathogens", "NameofStructureSystem": "Pathogens", "StructureNodeID": "99-00-00", "StructureGroupPath": "", "StructureGroupName": "", "StructureNodeStatus": "invalid" }, { "IdentifierofStructureSystem": "Health_Topics", "NameofStructureSystem": "Health Topics", "StructureNodeID": "99-00-00", "StructureGroupPath": "", "StructureGroupName": "", "StructureNodeStatus": "invalid" } ] } ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Language": "en", "Value": "Use Taq DNA Polymerase, 50 U/μl, especially for:
  • Setup of PCR master mixtures, when highly concentrated components are required
  • Preparation of dried amplification mixtures for more convenience and increased stability at ambient temperature

For further applications see Taq DNA Polymerase, GMP Grade, 5 U/μl", "Country": "XG", "Code": "Applications", "Name": "Applications" }, { "Language": "en", "Value": "Will be supplied as \"Taq DNA Pol., Glycerol-free\". Unit of measure is \"kU\".
The enzyme is supplied without reaction buffer.", "Country": "XG", "Code": "CB - Order Information", "Name": "CB - Order Information" }, { "Language": "en", "Value": "High concentrated, glycerol-free solution, ideal for preparation of dried-down amplification mixtures.", "Country": "XG", "Code": "Product Description", "Name": "Product Description" }, { "Language": "en", "Value": "Prepare dried amplification mixtures. Use this formulation for manufacture of dried-down reagents with high stability and convenience.", "Country": "XG", "Code": "Benefits", "Name": "Benefits" }, { "Language": "en", "Value": "Taq DNA Polymerase is the robust standard enzyme for the amplification of DNA fragments up to 3 kb in PCR, lyo ready formulation for preparation of dried amplification mixes.", "Country": "XG", "Code": "Positioning", "Name": "Positioning" }, { "Language": "en", "Value": "See Taq DNA Polymerase, GMP Grade, 5 U/μl", "Country": "XG", "Code": "Properties", "Name": "Properties" }, { "Language": "en", "Value": "Appearance: Clear, colorless solution
Storage buffer: Tris/HCl, 20 mmol/L; KCl, 100 mmol/L; DTT, 1 mmol/L; EDTA, 0.1 mmol/L; Nonidet P40, 0.5% (v/v); Tween 20, 0.5% (v/v); pH approximately 8.0 at +4°C
Glycerol content: ≤0.1% (v/v)
Volume activity: 55±5 U/μL
Unit definition: One unit Taq DNA Polymerase is defined as the amount of enzyme that incorporates 10 nmol of total deoxyribonucleosidetriphosphates into acid precipitable DNA within 30 minutes at +75°C under standard assay conditions.
Unspecific endonucleases (λDNA): Not detectable in up to 30 U after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 30 U after 16 hours incubation at +37°C.
Exonucleases (3H-DNA): Not detectable in up to 30 U after 4 hours incubation at +65°C.
Function test in qPCR using LightCycler® 480 System (≥3 ng of human genomic DNA, 339 bp tPA fragment): Corresponds to reference
Animal-derived additives: None
Stability: At -15 to -25°C within specification range for 24 months.", "Country": "XG", "Code": "Specification", "Name": "Specification" } ] } } ] }

Taq DNA Polymerase, 50 U/μl

from Thermus aquaticus BM, expressed in E. coli, glycerol-free solution

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