Others Taq DNA Polymerase 50 U uL Taq DNA Polymerase, 50 U/μl For customers in the European Economic Area: Contains SVHC: octyl/nonylphenol ethoxylates. For further processing on its own or in a mixture as part of an IVD method and under controlled conditions only – acc. to Art. 56 (3) and 3 no. 23 REACH Regulation. 3.6.17.2.1.8 from Thermus aquaticus BM, expressed in E. coli, glycerol-free solution Taq DNA Polymerase, 50 U/uL custom fill CustomBiotech product. Please contact your local representative. Will be supplied as "Taq DNA Pol., Glycerol-free". Unit of measure is "kU". The enzyme is supplied without reaction buffer. Taq DNA Pol., Glycerol-free 04827007103 Not Available Reagents, kits en Use Taq DNA Polymerase, 50 U/μl, especially for: Setup of PCR master mixtures, when highly concentrated components are required Preparation of dried amplification mixtures for more convenience and increased stability at ambient temperature For further applications see Taq DNA Polymerase, GMP Grade, 5 U/μl en High concentrated, glycerol-free solution, ideal for preparation of dried-down amplification mixtures. en Prepare dried amplification mixtures. Use this formulation for manufacture of dried-down reagents with high stability and convenience. en Taq DNA Polymerase is the robust standard enzyme for the amplification of DNA fragments up to 3 kb in PCR, lyo ready formulation for preparation of dried amplification mixes. en Appearance: Clear, colorless solution Storage buffer: Tris/HCl, 20 mmol/L; KCl, 100 mmol/L; DTT, 1 mmol/L; EDTA, 0.1 mmol/L; Nonidet P40, 0.5% (v/v); Tween 20, 0.5% (v/v); pH approximately 8.0 at +4°C Glycerol content: ≤0.1% (v/v) Volume activity: 55±5 U/μL Unit definition: One unit Taq DNA Polymerase is defined as the amount of enzyme that incorporates 10 nmol of total deoxyribonucleosidetriphosphates into acid precipitable DNA within 30 minutes at +75°C under standard assay conditions. Unspecific endonucleases (λDNA): Not detectable in up to 30 U after 16 hours incubation at +37°C. Nicking activity (pBR322 DNA): Not detectable in up to 30 U after 16 hours incubation at +37°C. Exonucleases (3H-DNA): Not detectable in up to 30 U after 4 hours incubation at +65°C. Function test in qPCR using LightCycler® 480 System (≥3 ng of human genomic DNA, 339 bp tPA fragment): Corresponds to reference Animal-derived additives: None Stability: At -15 to -25°C within specification range for 24 months.