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  • Setup of PCR master mixtures, when highly concentrated components are required
  • Preparation of dried amplification mixtures for more convenience and increased stability at ambient temperature

For further applications see Taq DNA Polymerase, GMP Grade, 5 U/μl", "Country": "XG", "Code": "Applications", "Name": "Applications" }, { "Language": "en", "Value": "Will be supplied as \"Taq DNA Pol., Glycerol-free\". Unit of measure is \"kU\".
The enzyme is supplied without reaction buffer.", "Country": "XG", "Code": "CB - Order Information", "Name": "CB - Order Information" }, { "Language": "en", "Value": "High concentrated, glycerol-free solution, ideal for preparation of dried-down amplification mixtures.", "Country": "XG", "Code": "Product Description", "Name": "Product Description" }, { "Language": "en", "Value": "Prepare dried amplification mixtures. Use this formulation for manufacture of dried-down reagents with high stability and convenience.", "Country": "XG", "Code": "Benefits", "Name": "Benefits" }, { "Language": "en", "Value": "Taq DNA Polymerase is the robust standard enzyme for the amplification of DNA fragments up to 3 kb in PCR, lyo ready formulation for preparation of dried amplification mixes.", "Country": "XG", "Code": "Positioning", "Name": "Positioning" }, { "Language": "en", "Value": "See Taq DNA Polymerase, GMP Grade, 5 U/μl", "Country": "XG", "Code": "Properties", "Name": "Properties" }, { "Language": "en", "Value": "Appearance: Clear, colorless solution
Storage buffer: Tris/HCl, 20 mmol/L; KCl, 100 mmol/L; DTT, 1 mmol/L; EDTA, 0.1 mmol/L; Nonidet P40, 0.5% (v/v); Tween 20, 0.5% (v/v); pH approximately 8.0 at +4°C
Glycerol content: ≤0.1% (v/v)
Volume activity: 55±5 U/μL
Unit definition: One unit Taq DNA Polymerase is defined as the amount of enzyme that incorporates 10 nmol of total deoxyribonucleosidetriphosphates into acid precipitable DNA within 30 minutes at +75°C under standard assay conditions.
Unspecific endonucleases (λDNA): Not detectable in up to 30 U after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 30 U after 16 hours incubation at +37°C.
Exonucleases (3H-DNA): Not detectable in up to 30 U after 4 hours incubation at +65°C.
Function test in qPCR using LightCycler® 480 System (≥3 ng of human genomic DNA, 339 bp tPA fragment): Corresponds to reference
Animal-derived additives: None
Stability: At -15 to -25°C within specification range for 24 months.", "Country": "XG", "Code": "Specification", "Name": "Specification" } ] } } ] }

Taq DNA Polymerase, 50 U/μl

from Thermus aquaticus BM, expressed in E. coli, glycerol-free solution

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