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• Routine PCR and RT-PCR applications
• Amplification of DNA fragments up to 3 kb from various sources of DNA
• Labeling of DNA with modified nucleotides (e.g., DIG-dUTP, biotin-dUTP, fluorescein-dUTP)
• Combination with dUTP and Uracil-DNA Glycosylase for prevention of carryover contamination between PCR reactions
• Manufacture of amplification mixtures for applications with regulatory requirements (e.g.,in vitro diagnostics, quality control)

", "Language": "en", "Country": "XG", "Code": "Applications" }, { "Name": "CB - Order Information", "Value": "Will be supplied as \"Taq DNA Polymerase\". Unit of measure is \"kU\".
The enzyme is supplied without reaction buffer.", "Language": "en", "Country": "XG", "Code": "CB - Order Information" }, { "Name": "Specification", "Value": "Appearance: Clear, colorless solution
Storage buffer: Tris/HCl, 20 mmol/L; KCl, 100 mmol/L; DTT, 1 mmol/L; EDTA, 0.1 mmol/L; Nonidet P40, 0.5% (v/v); Tween 20, 0.5% (v/v); glycerol, 50% (v/v), pH approximately 8.0 at +4°C
Volume activity: ≥5 U/μL
Unit definition: One unit Taq DNA polymerase is defined as the amount of enzyme that incorporates 10 nmol of total deoxyribonucleosidetriphosphates into acid precipitable DNA within 30 minutes at +75°C under standard assay conditions.
Unspecific endonucleases (λDNA): Not detectable in up to 30 U after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 30 U after 16 hours incubation at +37°C.
Exonucleases (³H-DNA): Not detectable in up to 30 U after 4 hours incubation at +65°C.
Function test in PCR using conventional blockcycler (10 pg λDNA, 0.5 kb fragment): Corresponds to reference
Animal-derived additives: None
Stability: At -15 to -25°C within specification range for 24 months.", "Language": "en", "Country": "XG", "Code": "Specification" }, { "Name": "Properties", "Value": "Taq DNA Polymerase is the recombinant full-length version of the thermostable enzyme from the eubacterium Thermus aquaticus BM, expressed in E. coli.
Enzyme acivities: Highly processive 5'-3' DNA polymerase; double-strand specific 5'-3' exonuclease; no 3'-5' exonuclease activity
pH optimum: Approximately 9.0 (+20°C)
Temperature optimum: Approximately +75°C
Half life at +95°C: Approximately 40 minutes
Substrates: Incorporates dNTP, dUPT, dITP, various labeled or modified nucleotides (200 μmol/L each is recommended of normal dNTP, increased concentrations of variants)
Divalent ion requirement: Mg²⁺ (1.5 mmol/L standard concentration)", "Language": "en", "Country": "XG", "Code": "Properties" } ] } } ] }