Others Taq DNA Polymerase 5 U uL Taq DNA Polymerase, 5 U/μl For further processing on its own or in a mixture as part of an IVD method only. 3.6.14.3.1.1 from Thermus aquaticus BM, expressed in E. coli, solution 11 147 633 103 11147633103 Taq DNA Polymerase Taq DNA Polymerase, 5 U/uL Reagents, kits custom fill 5 U/μL CustomBiotech product. Please contact your local representative. Will be supplied as "Taq DNA Polymerase". Unit of measure is "kU". The enzyme is supplied without reaction buffer. Taq DNA Polymerase is the robust standard enzyme for the amplification of DNA fragments up to 3 kb in PCR. en Use Taq DNA Polymerase, GMP Grade, 5 U/μl, for:Routine PCR and RT-PCR applicationsAmplification of DNA fragments up to 3 kb from various sources of DNALabeling of DNA with modified nucleotides (e.g., DIG-dUTP, biotin-dUTP, fluorescein-dUTP)Combination with dUTP and Uracil-DNA Glycosylase for prevention of carryover contamination between PCR reactionsManufacture of amplification mixtures for applications with regulatory requirements (e.g.,in vitro diagnostics, quality control) en Appearance: Clear, colorless solution Storage buffer: Tris/HCl, 20 mmol/L; KCl, 100 mmol/L; DTT, 1 mmol/L; EDTA, 0.1 mmol/L; Nonidet P40, 0.5% (v/v); Tween 20, 0.5% (v/v); glycerol, 50% (v/v), pH approximately 8.0 at +4°C Volume activity: ≥5 U/μL Unit definition: One unit Taq DNA polymerase is defined as the amount of enzyme that incorporates 10 nmol of total deoxyribonucleosidetriphosphates into acid precipitable DNA within 30 minutes at +75°C under standard assay conditions. Unspecific endonucleases (λDNA): Not detectable in up to 30 U after 16 hours incubation at +37°C. Nicking activity (pBR322 DNA): Not detectable in up to 30 U after 16 hours incubation at +37°C. Exonucleases (3H-DNA): Not detectable in up to 30 U after 4 hours incubation at +65°C. Function test in PCR using conventional blockcycler (10 pg λDNA, 0.5 kb fragment): Corresponds to reference Animal-derived additives: None Stability: At -15 to -25°C within specification range for 24 months. en

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Taq DNA Polymerase, 5 U/μl

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