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"Name": "Positioning",
"Value": "Taq DNA Polymerase is the robust standard enzyme for the amplification of DNA fragments up to 3 kb in PCR.",
"Language": "en",
"Country": "XG",
"Code": "Positioning"
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"Name": "Applications",
"Value": "Use Taq DNA Polymerase, GMP Grade, 5 U/μl, for:
- Routine PCR and RT-PCR applications
- Amplification of DNA fragments up to 3 kb from various sources of DNA
- Labeling of DNA with modified nucleotides (e.g., DIG-dUTP, biotin-dUTP, fluorescein-dUTP)
- Combination with dUTP and Uracil-DNA Glycosylase for prevention of carryover contamination between PCR reactions
- Manufacture of amplification mixtures for applications with regulatory requirements (e.g.,in vitro diagnostics, quality control)
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"Value": "
Appearance: Clear, colorless solution
Storage buffer: Tris/HCl, 20 mmol/L; KCl, 100 mmol/L; DTT, 1 mmol/L; EDTA, 0.1 mmol/L; Nonidet P40, 0.5% (v/v); Tween 20, 0.5% (v/v); glycerol, 50% (v/v), pH approximately 8.0 at +4°C
Volume activity: ≥5 U/μL
Unit definition: One unit Taq DNA polymerase is defined as the amount of enzyme that incorporates 10 nmol of total deoxyribonucleosidetriphosphates into acid precipitable DNA within 30 minutes at +75°C under standard assay conditions.
Unspecific endonucleases (λDNA): Not detectable in up to 30 U after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 30 U after 16 hours incubation at +37°C.
Exonucleases (
3H-DNA): Not detectable in up to 30 U after 4 hours incubation at +65°C.
Function test in PCR using conventional blockcycler (10 pg λDNA, 0.5 kb fragment): Corresponds to reference
Animal-derived additives: None
Stability: At -15 to -25°C within specification range for 24 months.",
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"Country": "XG",
"Code": "Specification"
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"Value": "Taq DNA Polymerase is the recombinant full-length version of the thermostable enzyme from the eubacterium
Thermus aquaticus BM, expressed in
E. coli.
Enzyme acivities: Highly processive 5'-3' DNA polymerase; double-strand specific 5'-3' exonuclease; no 3'-5' exonuclease activity
pH optimum: Approximately 9.0 (+20°C)
Temperature optimum: Approximately +75°C
Half life at +95°C: Approximately 40 minutes
Substrates: Incorporates dNTP, dUPT, dITP, various labeled or modified nucleotides (200 μmol/L each is recommended of normal dNTP, increased concentrations of variants)
Divalent ion requirement: Mg
2+ (1.5 mmol/L standard concentration)",
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