For further processing on its own or in a mixture as part of an IVD method only. Others Taq DNA Polymerase 5 U uL Taq DNA Polymerase, 5 U/μl 3.6.14.3.1.1 from Thermus aquaticus BM, expressed in E. coli, solution Taq DNA Polymerase is the robust standard enzyme for the amplification of DNA fragments up to 3 kb in PCR. en Use Taq DNA Polymerase, GMP Grade, 5 U/μl, for:Routine PCR and RT-PCR applicationsAmplification of DNA fragments up to 3 kb from various sources of DNALabeling of DNA with modified nucleotides (e.g., DIG-dUTP, biotin-dUTP, fluorescein-dUTP)Combination with dUTP and Uracil-DNA Glycosylase for prevention of carryover contamination between PCR reactionsManufacture of amplification mixtures for applications with regulatory requirements (e.g.,in vitro diagnostics, quality control) en Appearance: Clear, colorless solutionStorage buffer: Tris/HCl, 20 mmol/L; KCl, 100 mmol/L; DTT, 1 mmol/L; EDTA, 0.1 mmol/L; Tween 20, 0.5% (v/v); glycerol, 50% (v/v), pH approximately 8.0 at +4°CVolume activity: ≥5 U/μLUnit definition: One unit Taq DNA polymerase is defined as the amount of enzyme that incorporates 10 nmol of total deoxyribonucleosidetriphosphates into acid precipitable DNA within 30 minutes at +75°C under standard assay conditions.Unspecific endonucleases (λDNA): Not detectable in up to 30 U after 16 hours incubation at +37°C.Nicking activity (pBR322 DNA): Not detectable in up to 30 U after 16 hours incubation at +37°C.Exonucleases (3H-DNA): Not detectable in up to 30 U after 4 hours incubation at +65°C.Function test in PCR using conventional blockcycler (10 pg λDNA, 0.5 kb fragment): Corresponds to referenceAnimal-derived additives: NoneStability: At -15 to -25°C within specification range for 24 months. en

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Taq DNA Polymerase, 5 U/μl

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