For further processing only. Others T7 RNA Polymerase recombinant GMP Grade expressed in E. coli T7 RNA Polymerase, rec., GMP Grade, AOF 3.6.14.4.4.2.4 from T7 bacteriophage, recombinant in E.coli, solution 08 140 669 103 8 140 669 103 08140669103 8140669103 08140669103 T7 RNA Polymerase, rec., GMP Grade,10 ml T7 RNA Polymerase, recombinant, GMP Grade Reagents, kits 10 mL Not Available CustomBiotech product. Please contact your local representative. Will be supplied as "T7 RNA Polymerase, rec., GMP Grade". Unit of measure is "piece". EC 2.7.7.6 en T7 RNA Polymerase is the key enzyme for in vitro transcription of mRNA. en T7 RNA Polymerase generates mRNA from double-stranded DNA templates carrying the T7 promotor sequence.Previously used for many applications in R&D and some in Molecular Diagnostics, this key enzyme is now extensively used in the preparation of mRNA on commercial scale, for example, in the manufacture of COVID-19 vaccines. It efficiently incorporates standard or modified ribonucleoside triphosphates (NTPs) into mRNA transcripts. en Use the enzyme in the synthesis of mRNA for therapeutics or vaccines. It is designed to meet specific needs of the pharmaceutical industry (fit-for-purpose):animal-origin-free (AOF)free of antibioticsextended testing for impuritiesmanufactured under GMPsupplied with supplementary documents for regulatory submissions. en Appearance: Clear, colorless solutionVolume Activity: ≥ 1000 kU/mLUnit definition: One unit is defined as the enzyme activity which incorporates 1 nmol CMP in acid-precipitable RNA within 60 min at +37°C.Protein (abs 280nm): 0.8-1.2 mg/mLPurity (SDS-PAGE) ≥ 95 %Unspecific nucleases (up to 300 ng enzyme using MWM III-DNA; 16 h/37°C): Not detectableNicking activity (up to 300 ng enzyme using pBR 322-DNA; 16 h/37°C): Not detectableRNase activity ( up to 300 ng enzyme using MS II-RNA; 1 h/37°C): Not detectableProteases (50 μg/15 min/37°C): Not detectableExonucleases (using 1 μg): Not detectableEndotoxins: ≤ 10 EU/mLTotal Bioburden: ≤ 10 CFU/mLE.coli HC DNA ≤ 100 pg/mg protein: CorrespondsE.coli HC protein ≤ 50 ppm: CorrespondsHeavy metals (Ni, Co, V): ≤ 10 ppmStability: At -25°C to -15°C within specification range for 24 months from date of manufacture. en

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T7 RNA Polymerase, rec., GMP Grade, AOF

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