{ "ProductData": { "RelatedLinks": "", "RegulatoryDisclaimer1": "For further processing only.", "RegulatoryDisclaimer2": "", "StainLocalization": [ "" ], "DisclaimerGroup2": null, "ProductNameGlobal": "T7 RNA Polymerase recombinant GMP Grade expressed in E. coli", "DisclaimerGroup1": "Other - Non IVD", "ControlTissue": [ "" ], "BrandName": "T7 RNA Polymerase, recombinant, GMP Grade", "LicenseDisclaimers": [ ], "Isotypes": "", "RegulatoryDisclaimer3": "", "ID": "3.6.14.4.4.2.4", "ProductNameAddition": "expressed in E. coli", "Species": [ "" ], "Clone": "" }, "ProductSpec": { "ProductSpecVariant": { "Chapters": [ { "Language": "English", "Value": "Will be supplied as \"T7 RNA Polymerase, rec., GMP Grade\". Unit of measure is \"piece\".", "Name": "CB - Order Information" }, { "Language": "English", "Value": "Appearance: Clear, colorless solution
Volume Activity: ≥ 1000 kU/mL
Protein (abs 280nm): 0.8-1.2 mg/mL
Purity (SDS-PAGE) ≥ 95 %
Unspecific nucleases (up to 300 ng enzyme using MWM III-DNA; 16 h/37°C): not detectable
Nicking activity (up to 300 ng enzyme using pBR 322-DNA; 16 h/37°C): not detectable
RNase activity ( up to 300 ng enzyme using MS II-RNA; 1 h/37°C): not detectable
Proteases (50 μg/15 min/37°C): not detectable
Exonucleases (using 1 μg): not detectable
Endotoxins: ≤ 10 EU/mL
Total Bioburden: ≤ 10 CFU/mL
E.coli HC DNA ≤ 100 pg/mg protein: corresponds
E.coli HC protein ≤ 50 ppm: corresponds
Heavy metals (Ni, Co, V): ≤ 10 ppm
Stability: At -25°C to -15°C within specification range for 18 months.", "Name": "CB - Specification" }, { "Language": "English", "Value": "T7 RNA Polymerase exhibits high specificity for T7 promoter sequence and requires Mg²⁺ as a cofactor for the synthesis. The enzyme efficiently incorporates standard or modified nucleoside triphosphates (NTPs) into RNA transcripts.", "Name": "Product Description" }, { "Language": "English", "Value": "T7 RNA Polymerase is used for in vitro transcription of RNA from a DNA template.", "Name": "Positioning" }, { "Language": "English", "Value": "It is the key enzyme for in vitro transcription of mRNA. It is designed fit-for-purpose to meet the manufacturing needs of therapeutic mRNA.", "Name": "Applications" }, { "Language": "English", "Value": "Using fit-for-purpose raw materials enables you to focus on drug design and clinical studies, and increase certainty in your manufacturing process.

Roche T7 RNA Polymerase is

• manufactured in a state-of-the-art facility under GMP quality standards
• animal-free and ß-lactam antibiotic-free
• available in scale and quality for every stage — from development to manufacturing
• backed by the expertise of a global pioneer in diagnostics and pharmaceuticals

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Volume Activity: ≥ 1000 kU/mL
Protein (abs 280nm): 0.8-1.2 mg/mL
Purity (SDS-PAGE) ≥ 95 %
Unspecific nucleases (up to 300 ng enzyme using MWM III-DNA; 16 h/37°C): not detectable
Nicking activity (up to 300 ng enzyme using pBR 322-DNA; 16 h/37°C): not detectable
RNase activity ( up to 300 ng enzyme using MS II-RNA; 1 h/37°C): not detectable
Proteases (50 μg/15 min/37°C): not detectable
Exonucleases (using 1 μg): not detectable
Endotoxins: ≤ 10 EU/mL
Total Bioburden: ≤ 10 CFU/mL
E.coli HC DNA ≤ 100 pg/mg protein: corresponds
E.coli HC protein ≤ 50 ppm: corresponds
Heavy metals (Ni, Co, V): ≤ 10 ppm
Stability: At -25°C to -15°C within specification range for 18 months.", "Name": "Specification" }, { "Language": "English", "Value": "Molecular weight: 98 kD
Supplied as solution", "Name": "Properties" } ] } }, "Product2Taxomony": { "Product2TaxomonyReferences": [ { "StructureSystemName": "OWP Product Solutions", "NodeName": "CustomBiotech", "StructureSystemID": null, "NodeID": "520" }, { "StructureSystemName": "Product Grouping", "NodeName": "CustomBiotech->mRNA Reagents", "StructureSystemID": null, "NodeID": "02-0062" }, { "StructureSystemName": "OWP Product Types", "NodeName": "Raw Materials and Reagents->DNA Polymerases", "StructureSystemID": null, "NodeID": "70-042-00" }, { "StructureSystemName": "Product Grouping", "NodeName": "CustomBiotech->Cell & Gene Therapy", "StructureSystemID": null, "NodeID": "02-0133" }, { "StructureSystemName": "OWP Organization", "NodeName": "Roche Diagnostics Solution (RDS)->Customer Areas->Core lab->CustomBiotech and LifeScience", "StructureSystemID": null, "NodeID": "01-01-01-07-00" }, { "StructureSystemName": "Product Grouping", "NodeName": "CustomBiotech->Enzymes", "StructureSystemID": null, "NodeID": "02-0014" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "08140669103", "MaterialDescription": "T7 RNA Polymerase, rec., GMP Grade,10 ml", "RegisteredProductName": "", "GTIN": "", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "10 mL", "PackSizeDescPIM360": "", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"T7 RNA Polymerase, rec., GMP Grade\". Unit of measure is \"piece\"." } ] }, "Product2Products": { "Product2ProductReference": [ ] } }