For further processing only. Others T4 DNA Ligase solution T4 DNA Ligase 3.6.14.2.2.2 recombinant form of the enzyme from T4 phage, solution 10909246103 DNA Ligase, T4, rec T4 DNA Ligase Reagents, kits custom fill Not Available CustomBiotech product. Please contact your local representative. Will be supplied as "DNA Ligase, T4 from T4-infected EcoNM989". Unit of measure is "kU". The enzyme is supplied without reaction buffer. EC 6.5.1.1 en 9015-85-4 en Use T4 DNA Ligase to ligate DNA fragments with blunt or overlapping ends. en Use T4 DNA Ligase for ligation of DNA fragments. en Appearance: Clear, colorless solutionStorage buffer: Tris/HCl, 20 mmol/L; KCl, 60 mmol/L; DTE, 5 mmol/L; EDTA, 1 mmol/L; glycerol, 50% (v/v); pH approximately 7.5 at +4°CVolume activity: ≥5 U/μLUnit definition: One unit T4 DNA Ligase is defined as the amount of enzyme which converts 1 nmol of [32P] from pyrophosphate into Norit-absorbable material in 20 minutes at +37°C.Glycosylases (M13mp11(U) ssDNA): Not detectable in up to 10 U after 16 hours incubation at +37°C.Nicking activity (pBR322 DNA): Not detectable in up to 10 U after 16 hours incubation at +37°C.Animal-derived additives: NoneStability: At -15 to -25°C within specification range for 18 months. en