{
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"ProductType": "Others",
"BrandName": "T4 DNA Ligase",
"ProductNameAddition": "recombinant form of the enzyme from T4 phage, solution",
"RegulatoryDisclaimer1": "For further processing only.",
"DisclaimerGroup1": "",
"RegulatoryDisclaimer2": null,
"DisclaimerGroup2": null,
"RegulatoryDisclaimer3": null,
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"RelatedLinks": "",
"Clone": "",
"ControlTissue": [
""
],
"ISOtypes": "",
"Species": [
""
],
"StainLocalization": [
""
],
"ProductNameGlobal": "T4 DNA Ligase solution"
},
"ProductImageDetails": {
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"ImageType": "Image main"
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"StructureSystemName": "Product Grouping",
"StructureNodeStatus": "valid",
"NodeName": "Molecular Raw Materials",
"NodeID": "02-0136"
},
{
"StructureSystemIdentifier": "OWP_Product_Types",
"StructureSystemName": "Product Types",
"StructureNodeStatus": "valid",
"NodeName": "Ligases",
"NodeID": "70-033-00"
},
{
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"StructureSystemName": "Organization",
"StructureNodeStatus": "valid",
"NodeName": "CustomBiotech and LifeScience",
"NodeID": "01-01-01-07-00"
},
{
"StructureSystemIdentifier": "Product_Grouping",
"StructureSystemName": "Product Grouping",
"StructureNodeStatus": "valid",
"NodeName": "Enzymes",
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"Product2Materials": {
"P2MaterialReferences": [
{
"MaterialNum": "10909246103",
"MaterialDescription": "DNA Ligase, T4, rec",
"RegisteredProductName": "T4 DNA Ligase",
"GTIN": "",
"ProductCategoryText": "Reagents, kits",
"OldMaterialNumber": "",
"PackSizePIM360": "custom fill",
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"ReadyForUse": "",
"OrderInformation": "Will be supplied as \"DNA Ligase, T4 from T4-infected EcoNM989\". Unit of measure is \"kU\".
The enzyme is supplied without reaction buffer."
}
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},
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"ProductSpec": [
{
"ProductSpecVariant": {
"Chapters": [
{
"Language": "en",
"Value": "Will be supplied as \"DNA Ligase, T4 from T4-infected EcoNM989\". Unit of measure is \"kU\".
The enzyme is supplied without reaction buffer.",
"Country": "XG",
"Code": "CB - Order Information",
"Name": "CB - Order Information"
},
{
"Language": "en",
"Value": "Use T4 DNA Ligase to ligate DNA fragments with blunt or overlapping ends.",
"Country": "XG",
"Code": "Applications",
"Name": "Applications"
},
{
"Language": "en",
"Value": "Use T4 DNA Ligase for ligation of DNA fragments.",
"Country": "XG",
"Code": "Positioning",
"Name": "Positioning"
},
{
"Language": "en",
"Value": "
Appearance: Clear, colorless solution
Storage buffer: Tris/HCl, 20 mmol/L; KCl, 60 mmol/L; DTE, 5 mmol/L; EDTA, 1 mmol/L; glycerol, 50% (v/v); pH approximately 7.5 at +4°C
Volume activity: ≥5 U/μL
Unit definition: One unit T4 DNA Ligase is defined as the amount of enzyme which converts 1 nmol of [
32P] from pyrophosphate into Norit-absorbable material in 20 minutes at +37°C.
Glycosylases (M13mp11(U) ssDNA): Not detectable in up to 10 U after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 10 U after 16 hours incubation at +37°C.
Animal-derived additives: None
Stability: At -15 to -25°C within specification range for 18 months.",
"Country": "XG",
"Code": "Specification",
"Name": "Specification"
},
{
"Language": "en",
"Value": "
CAS Number: 9015-85-4
Enzyme activities: T4 DNA Ligase catalyzes the formation of phosphodiester bonds between neighbouring 3'-hydroxyl and 5'-phosphate ends in double-stranded DNA. Sticky- and blunt-ended DNA fragments are ligated. Single-stranded nicks in double-stranded DNA are also closed.
Appropriate ligation buffer, 10x concentrated: Tris/HCl, 660 mmol/L; MgCl
2, 50 mmol/L; DTT, 50 mmol/L; ATP, 10 mmol/L, pH 7.5 at +20°C (Note: ATP is not stable).
pH optimum: 7.2-7.8
Divalent ion requirement: Mg
2+Inactivation: After 10 minutes heat denaturation at +65°C ligase activity is stopped.",
"Country": "XG",
"Code": "Properties",
"Name": "Properties"
}
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}
}
]
}