{ "ProductData": { "ID": "3.6.14.2.2.2", "ProductType": "Others", "BrandName": "T4 DNA Ligase", "ProductNameAddition": "recombinant form of the enzyme from T4 phage, solution", "RegulatoryDisclaimer1": "For further processing only.", "DisclaimerGroup1": "", "RegulatoryDisclaimer2": null, "DisclaimerGroup2": null, "RegulatoryDisclaimer3": null, "SampleType": [ ], "LicenseDisclaimers": [ ], "RelatedLinks": "", "Clone": "", "ControlTissue": [ "" ], "ISOtypes": "", "Species": [ "" ], "StainLocalization": [ "" ], "ProductNameGlobal": "T4 DNA Ligase solution" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/CB_IMAGE.png", "ImageType": "Image main" }, "Product2Taxonomy": { "Product2TaxonomyReferences": [ { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "StructureNodeStatus": "valid", "NodeName": "Molecular Raw Materials", "NodeID": "02-0136" }, { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "StructureNodeStatus": "valid", "NodeName": "Ligases", "NodeID": "70-033-00" }, { "StructureSystemIdentifier": "OWP_Organization", "StructureSystemName": "Organization", "StructureNodeStatus": "valid", "NodeName": "CustomBiotech and LifeScience", "NodeID": "01-01-01-07-00" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "StructureNodeStatus": "valid", "NodeName": "Enzymes", "NodeID": "02-0014" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "StructureNodeStatus": "valid", "NodeName": "CustomBiotech", "NodeID": "520" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "180-00" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "999-00" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "999" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "99-00-00" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "10909246103", "MaterialDescription": "DNA Ligase, T4, rec", "RegisteredProductName": "T4 DNA Ligase", "GTIN": "", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "custom fill", "PackSizeDescPIM360": "Not Available", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"DNA Ligase, T4 from T4-infected EcoNM989\". Unit of measure is \"kU\".
The enzyme is supplied without reaction buffer." } ] }, "Product2Products": { "Product2ProductReference": [ ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Language": "en", "Value": "Will be supplied as \"DNA Ligase, T4 from T4-infected EcoNM989\". Unit of measure is \"kU\".
The enzyme is supplied without reaction buffer.", "Country": "XG", "Code": "CB - Order Information", "Name": "CB - Order Information" }, { "Language": "en", "Value": "Use T4 DNA Ligase to ligate DNA fragments with blunt or overlapping ends.", "Country": "XG", "Code": "Applications", "Name": "Applications" }, { "Language": "en", "Value": "Use T4 DNA Ligase for ligation of DNA fragments.", "Country": "XG", "Code": "Positioning", "Name": "Positioning" }, { "Language": "en", "Value": "Appearance: Clear, colorless solution
Storage buffer: Tris/HCl, 20 mmol/L; KCl, 60 mmol/L; DTE, 5 mmol/L; EDTA, 1 mmol/L; glycerol, 50% (v/v); pH approximately 7.5 at +4°C
Volume activity: ≥5 U/μL
Unit definition: One unit T4 DNA Ligase is defined as the amount of enzyme which converts 1 nmol of [³²P] from pyrophosphate into Norit-absorbable material in 20 minutes at +37°C.
Glycosylases (M13mp11(U) ssDNA): Not detectable in up to 10 U after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 10 U after 16 hours incubation at +37°C.
Animal-derived additives: None
Stability: At -15 to -25°C within specification range for 18 months.", "Country": "XG", "Code": "Specification", "Name": "Specification" }, { "Language": "en", "Value": "CAS Number: 9015-85-4
Enzyme activities: T4 DNA Ligase catalyzes the formation of phosphodiester bonds between neighbouring 3'-hydroxyl and 5'-phosphate ends in double-stranded DNA. Sticky- and blunt-ended DNA fragments are ligated. Single-stranded nicks in double-stranded DNA are also closed.
Appropriate ligation buffer, 10x concentrated: Tris/HCl, 660 mmol/L; MgCl₂, 50 mmol/L; DTT, 50 mmol/L; ATP, 10 mmol/L, pH 7.5 at +20°C (Note: ATP is not stable).
pH optimum: 7.2-7.8
Divalent ion requirement: Mg²⁺
Inactivation: After 10 minutes heat denaturation at +65°C ligase activity is stopped.", "Country": "XG", "Code": "Properties", "Name": "Properties" } ] } } ] }