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"Storage Conditions (Product)" }, { "Name": "Additional Equipment and Reagent Required", "Value": "
Analyzer and accessories
  • Cedex Bio Analyzer*, including accessories and disposables
  • Standard laboratory equipment
Calibrators and Controls
  • Calibrator A Bio*
  • Control D Level 1 Bio*
  • Control D Level 2 Bio*
  • Control D Level 3 Bio*
Optional for correction of glucose background
  • Glucose Bio 
  • Control A Level 1 Bio* 
  • Control A Level 2 Bio* 
  • Control A Level 3 Bio* 
", "Language": "en", "Country": "XG", "Code": "Additional Equipment and Reagent Required" }, { "Name": "CB - Order Information", "Value": "Unit of measure is \"piece\".", "Language": "en", "Country": "XG", "Code": "CB - Order Information" }, { "Name": "Product Description", "Value": "Kit for determination of sucrose", "Language": "en", "Country": "XG", "Code": "Product Description" }, { "Name": "Content", "Value": "
VialCapContentComposition
1 SUCB R1white4 vials,
50 tests each
Reagent 1 (R1): MES, 5.0 mmol/L; adenosine triphosphate, 4.5 mmol/L; Mg2+, 24 mmol/L; NADP, ≥7 mmol/L; β-fructosidase, ≥140 kU/L; pH 6.0.
2 SUCB SRblack4 vials,
50 tests each
Start Reagent (SR): HEPES, 200 mmol/L; Mg2+, 4 mmol/L; hexokinase (HK), ≥300 μkat/L; G6PDH, ≥300 μkat/L; pH 8.0.
", "Language": "en", "Country": "XG", "Code": "Content" }, { "Name": "Applications", "Value": "Sucrose can be used as source of carbon and energy in cell cultures. Use of sucrose instead of glucose in the culture medium offers the advantage of lower osmolality (based on gramm of carbohydrate). Furthermore, producing therapeutic antibodies, the use of sucrose in the cell culture affects the oligosaccharide maturation on the recombinant proteins, resulting in an increase in the percentage of high mannose N-glycan species, as well as a concomitant reduction in fucosylation, which increases the antibody-dependent cell-mediated cytotoxicity for the antibody. For control of culture media and for monitoring in a running culture process, the sucrose concentration can be determined on a Cedex Bio or Cedex Bio HT Analyzer with high reliability and convenience.", "Language": "en", "Country": "XG", "Code": "Applications" }, { "Name": "Specification", "Value": "Method: Enzymatic photometric assay using hydrolysis by invertase and phosphorylation of glucose by hexokinase.
Recovery: ±15%", "Language": "en", "Country": "XG", "Code": "Specification" } ] } } ] }

Sucrose Bio

Test kit for the Cedex Bio Analyzer

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Technical documents

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