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"ProductNameAddition": "sodium salt, 100 mM, solution", "ReferenceType": "Reagent", "Classification": [ { "IdentifierofStructureSystem": "Lab_Type", "NameofStructureSystem": "Lab Types", "StructureNodeID": "180-00", "StructureGroupPath": "Manufacturing", "StructureGroupName": "Manufacturing" }, { "IdentifierofStructureSystem": "OWP_Product_Types", "NameofStructureSystem": "Product Types", "StructureNodeID": "70-038-00", "StructureGroupPath": "Raw Materials->Nucleic Acids", "StructureGroupName": "Nucleic Acids" }, { "IdentifierofStructureSystem": "Product_Solutions", "NameofStructureSystem": "Product Solutions", "StructureNodeID": "520", "StructureGroupPath": "CustomBiotech", "StructureGroupName": "CustomBiotech" }, { "IdentifierofStructureSystem": "Applications", "NameofStructureSystem": "Applications", "StructureNodeID": "99-00-00", "StructureGroupPath": "NOT APPLICABLE", "StructureGroupName": "NOT APPLICABLE" }, { "IdentifierofStructureSystem": "Disease_Areas", "NameofStructureSystem": "Disease Areas", "StructureNodeID": "99-00-00", "StructureGroupPath": "NOT APPLICABLE", "StructureGroupName": "NOT APPLICABLE" }, { "IdentifierofStructureSystem": "Pathogens", "NameofStructureSystem": "Pathogens", "StructureNodeID": "99-00-00", "StructureGroupPath": "NOT APPLICABLE", "StructureGroupName": "NOT APPLICABLE" }, { "IdentifierofStructureSystem": "OWP_Family", "NameofStructureSystem": "Product Families", "StructureNodeID": "999", "StructureGroupPath": "NOT APPLICABLE", "StructureGroupName": "NOT APPLICABLE" }, { "IdentifierofStructureSystem": "Health_Topics", "NameofStructureSystem": "Health Topics", "StructureNodeID": "99-00-00", "StructureGroupPath": "NOT APPLICABLE", "StructureGroupName": "NOT APPLICABLE" }, { "IdentifierofStructureSystem": "Product_Grouping", "NameofStructureSystem": "Product Grouping", "StructureNodeID": "02-0090", "StructureGroupPath": "CustomBiotech->Vaccine Testing", "StructureGroupName": "Vaccine Testing" }, { "IdentifierofStructureSystem": "Product_Grouping", "NameofStructureSystem": "Product Grouping", "StructureNodeID": "02-0062", "StructureGroupPath": "CustomBiotech->mRNA Reagents", "StructureGroupName": "mRNA Reagents" } ] } ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Language": "en", "Value": "The enzyme is provided in CO2-proof bottles to ensure a stable pH during shipment on dry ice.", "Country": "XG", "Code": "Storage Conditions (Product)", "Name": "Storage Conditions (Product)" }, { "Language": "en", "Value": "SP6 RNA Polymerase is one of the key enzymes for the in vitro synthesis of mRNA from a DNA template.", "Country": "XG", "Code": "Positioning", "Name": "Positioning" }, { "Language": "en", "Value": "SP6 RNA Polymerase transcribes RNA from double-stranded DNA templates that are downstream from an SP6 promoter. Roche SP6 is a proven enzyme in many R&D and Molecular Diagnostics applications. This key enzyme is now extensively used also in the preparation of mRNA on commercial scale, for example, in the manufacturing of mRNA vaccines. It efficiently incorporates standard or modified ribonucleoside triphosphates (NTPs) into mRNA transcripts.", "Country": "XG", "Code": "Applications", "Name": "Applications" }, { "Language": "en", "Value": "EC Number: 2.7.7.6
Molecular weight: 98.5 kD
Cofactor requirement: Mg2+ for RNA synthesis
SP6 promoter sequence required", "Country": "XG", "Code": "Properties", "Name": "Properties" }, { "Language": "en", "Value": "Appearance: Clear, colorless solution
Volume Activity: ≥500 kU/mL
Protein (abs 280nm): 1.8 – 2.2 mg/ml 
Purity (SDS-PAGE): ≥95 % 
Identity: Confirmed
Unspecific nucleases (up to 300 ng enzyme using MWM III-DNA; 16 h/37°C): Not detectable
Nicking activity (up to 300 ng enzyme using pBR 322-DNA; 16 h/37°C): Not detectable
RNase activity (up to 300 ng enzyme using MS II-RNA; 1 h/37°C): Not detectable
Proteases (50 μg/15 min/37°C): Not detectable
Exonucleases (using 1 μg): Not detectable
Endotoxins: <5 EU/mL
Total Bioburden: <5 CFU/mL
E.coli HC DNA ≤100 pg/mg protein: Corresponds
E.coli HC protein ≤ 50 ppm: Corresponds
Heavy metals (Ni, Co, V): ≤ 10 ppm
Stability: At -25°C to -15°C within specification range for 18 months from date of manufacture.", "Country": "XG", "Code": "Specification", "Name": "Specification" }, { "Language": "en", "Value": "Will be supplied as \"SP6 RNA Polymerase, rec., AOF\". Unit of measure is \"mL\".", "Country": "XG", "Code": "CB - Order Information", "Name": "CB - Order Information" }, { "Language": "en", "Value": "Use the enzyme in the synthesis of mRNA for therapeutics or vaccines. It is designed to meet specific needs of the pharmaceutical industry (fit-for-purpose):
  • animal-origin-free (AOF)
  • free of antibiotics
  • extended testing for impurities
  • supplied with supplementary documents for regulatory submissions.
", "Country": "XG", "Code": "Benefits", "Name": "Benefits" } ] } } ] }

SP6 RNA Polymerase, rec., AOF

from SP6 bacteriophage, recombinant in E.coli, solution

cb logo
mrna

For an efficient mRNA synthesis
 

SP6 RNA Polymerase is one of the key enzymes for in vitro synthesis of mRNA. Developed in our fit-for-purpose quality, CustomBiotech SP6 RNA Polymerase provides the performance, quality and scale you need, to synthesize mRNA for therapeutics and vaccines manufacturing.

 

We have designed our manufacturing process from the master cell bank to final QC-release to exclude or minimize potential contaminants, thereby reducing overall process risk and simplifying procedures for regulatory approval.

from the generation of the master cell bank to the final filling, noanimal-derived materials are used in the process. This greatly simplifies your risk analysis.

our proprietary E. coli strain eliminates the need for antibiotics during fermentation. Therefore, allergic reactions to antibiotics is not a factor in your overall risk analysis.

tested during final QC-release for the absence of impurities such as nucleases, host-cell DNA and protein, bioburden, heavy metals, or endotoxins. Work with an enzyme at peak performance that poses no risk to downstream processing.

We work with you to provide the necessary technical documents for your regulatory submissions.

Changing reagent quality between pre-clinical development and commercialization poses a risk. Secure your processes by using the best available quality from the start.

Reach out to your regional representative for a quote or sample
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Ordering Information

Technical documents

for certificates, method sheets (instructions for use), safety data sheets and software downloads
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