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double-stranded DNA templates that are downstream from an SP6 promoter. Roche SP6 is a proven enzyme in many R&D and Molecular Diagnostics applications. This key enzyme is now extensively used also in the preparation of mRNA on commercial scale, for example, in the manufacturing of mRNA vaccines and therapeutics. It efficiently incorporates standard or modified ribonucleoside triphosphates (NTPs) into mRNA transcripts.", "Language": "en", "Country": "XG", "Code": "Applications" }, { "Name": "Benefits", "Value": "We have designed our manufacturing process from the master cell bank to final QC-release to exclude or minimize potential contaminants, thereby reducing overall process risk and simplifying procedures for regulatory approval.

  • GMP Grade quality
  • Animal-Origin-Free process (AOF)
  • Antibiotics-free process
  • Extended testing for impurities
  • Testing of Heavy Metals as contaminant
  • Regulatory document support
", "Language": "en", "Country": "XG", "Code": "Benefits" }, { "Name": "Properties", "Value": "EC Number: 2.7.7.6
Molecular weight: 96 kD
Cofactor requirement: Mg2+ for RNA synthesis
SP6 promoter sequence required", "Language": "en", "Country": "XG", "Code": "Properties" }, { "Name": "Specification", "Value": "Appearance: Clear, colorless solution
Volume Activity: ≥ 500 kU/mL
Unit definition: One unit is defined as the enzyme activity which incorporates 1 nmol CMP in acid-precipitable RNA within 60 min at +37°C
Protein (abs 280 nm): 1.8 – 2.2 mg/ml
Purity (SDS-PAGE): ≥ 95 %
Unspecific nucleases (up to 300 ng enzyme using MWM III-DNA; 16 h/37°C): Not detectable
Nicking activity (up to 300 ng enzyme using pBR 322-DNA; 16 h/37°C): Not detectable
RNase activity ( up to 300 ng enzyme using MS II-RNA; 1 h/37°C): Not detectable
Proteases (50 μg/15 min/37°C): Not detectable
Exonucleases (using 1 μg): Not detectable
Endotoxins: <5 EU/mL
Total Bioburden: <5 CFU/mL
E.coli HC DNA ≤ 100 pg/mg protein: Corresponds
E.coli HC protein ≤ 50 ppm: Corresponds
Heavy metals (Ni, Co, V): ≤ 10 ppm
Stability: At -25°C to -15°C within specification range for 18 months from date of manufacture.", "Language": "en", "Country": "XG", "Code": "Specification" }, { "Name": "CB - Order Information", "Value": "Will be supplied as 'SP6 RNA Polymerase, rec., GMP Grade, AOF'. Unit of measure is 'mL'.", "Language": "en", "Country": "XG", "Code": "CB - Order Information" } ] } } ] }

SP6 RNA Polymerase, rec., GMP Grade

from SP6 bacteriophage, recombinant in E.coli, solution

SP6 RNA Polymerase, rec., GMP Grade
For an efficient mRNA synthesis

SP6 RNA Polymerase is one of the key enzymes for in vitro synthesis of mRNA. Developed in our fit-for-purpose quality, CustomBiotech SP6 RNA Polymerase provides the performance, quality and scale you need, to synthesize mRNA for therapeutics and vaccines manufacturing.

We have designed our manufacturing process from the master cell bank to final QC-release to exclude or minimize potential contaminants, thereby reducing overall process risk and simplifying procedures for regulatory approval.

Produced using validated manufacturing and cleaning processes up to the final filling step and released under stringent final QC checks. Know that you are getting consistent quality every time.

From the generation of the master cell bank to the final filling, noanimal-derived materials are used in the process. This greatly simplifies your risk analysis.

Our proprietary E. coli strain eliminates the need for antibiotics during fermentation. Therefore, allergic reactions to antibiotics is not a factor in your overall risk analysis.

tested during final QC-release for the absence of impurities such as nucleases, host-cell DNA and protein, bioburden, heavy metals, or endotoxins. Work with an enzyme at peak performance that poses no risk to downstream processing.

We work with you to provide the necessary technical documents for your regulatory submissions.

Changing reagent quality between pre-clinical development and commercialization poses a risk. Secure your processes by using the best available quality from the start.

Reach out to your regional representative for a quote or sample
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Ordering Information

Technical documents

for certificates, method sheets (instructions for use), safety data sheets and software downloads
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