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double-stranded DNA templates that are downstream from an SP6 promoter. Roche SP6 is a proven enzyme in many R&D and Molecular Diagnostics applications. This key enzyme is now extensively used also in the preparation of mRNA on commercial scale, for example, in the manufacturing of mRNA vaccines and therapeutics. It efficiently incorporates standard or modified ribonucleoside triphosphates (NTPs) into mRNA transcripts.", "Language": "en", "Country": "XG", "Code": "Applications" }, { "Name": "Benefits", "Value": "We have designed our manufacturing process from the master cell bank to final QC-release to exclude or minimize potential contaminants, thereby reducing overall process risk and simplifying procedures for regulatory approval.

• GMP Grade quality
• Animal-Origin-Free process (AOF)
• Antibiotics-free process
• Extended testing for impurities
• Testing of Heavy Metals as contaminant
• Regulatory document support

", "Language": "en", "Country": "XG", "Code": "Benefits" }, { "Name": "Properties", "Value": "EC Number: 2.7.7.6
Molecular weight: 96 kD
Cofactor requirement: Mg²⁺ for RNA synthesis
SP6 promoter sequence required", "Language": "en", "Country": "XG", "Code": "Properties" }, { "Name": "Specification", "Value": "Appearance: Clear, colorless solution
Volume Activity: ≥ 500 kU/mL
Unit definition: One unit is defined as the enzyme activity which incorporates 1 nmol CMP in acid-precipitable RNA within 60 min at +37°C
Protein (abs 280 nm): 1.8 – 2.2 mg/ml
Purity (SDS-PAGE): ≥ 95 %
Unspecific nucleases (up to 300 ng enzyme using MWM III-DNA; 16 h/37°C): Not detectable
Nicking activity (up to 300 ng enzyme using pBR 322-DNA; 16 h/37°C): Not detectable
RNase activity ( up to 300 ng enzyme using MS II-RNA; 1 h/37°C): Not detectable
Proteases (50 μg/15 min/37°C): Not detectable
Exonucleases (using 1 μg): Not detectable
Endotoxins: <5 EU/mL
Total Bioburden: <5 CFU/mL
E.coli HC DNA ≤ 100 pg/mg protein: Corresponds
E.coli HC protein ≤ 50 ppm: Corresponds
Heavy metals (Ni, Co, V): ≤ 10 ppm
Stability: At -25°C to -15°C within specification range for 18 months from date of manufacture.", "Language": "en", "Country": "XG", "Code": "Specification" }, { "Name": "CB - Order Information", "Value": "Will be supplied as 'SP6 RNA Polymerase, rec., GMP Grade, AOF'. Unit of measure is 'mL'.", "Language": "en", "Country": "XG", "Code": "CB - Order Information" } ] } } ] }