{ "ProductData": { "ID": "PID00000122", "ProductType": "Others", "BrandName": "Restriction endonuclease Xba I, recombinant, AOF", "ProductNameAddition": "from Xanthomonas campestris pv. Badrii, expressed in E. coli, solution", "RegulatoryDisclaimer1": "For further processing only.", "DisclaimerGroup1": "", "RegulatoryDisclaimer2": null, "DisclaimerGroup2": null, "RegulatoryDisclaimer3": null, "SampleType": [ ], "LicenseDisclaimers": [ ], "RelatedLinks": "", "Clone": "", "ControlTissue": [ "" ], "ISOtypes": "", "Species": [ "" ], "StainLocalization": [ "" ], "ProductNameGlobal": "Restriction endonuclease Xba I recombinant AOF" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/CB_IMAGE.png", "ImageType": "Image main" }, "Product2Taxonomy": { "Product2TaxonomyReferences": [ { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "StructureNodeStatus": "valid", "NodeName": "Enzymes", "NodeID": "02-0014" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "StructureNodeStatus": "valid", "NodeName": "CustomBiotech", "NodeID": "520" }, { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "StructureNodeStatus": "valid", "NodeName": "Restriction Enzymes", "NodeID": "70-062-00" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "StructureNodeStatus": "valid", "NodeName": "mRNA Reagents", "NodeID": "02-0062" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "180-00" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "999-00" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "999" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "StructureNodeStatus": "valid", "NodeName": "Vaccine Testing", "NodeID": "02-0090" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "StructureNodeStatus": "valid", "NodeName": "Cell and Gene Therapy", "NodeID": "02-0133" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "09520848101", "MaterialDescription": "RE Xba I rec., 50 kU", "RegisteredProductName": "Restriction endonuclease Xba I, recombinant, AOF", "GTIN": "", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "50 kU  (5 mL)", "PackSizeDescPIM360": "Not Available", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"RE Xba I rec., 50 kU\". Unit of measure is \"piece\"." }, { "MaterialNum": "09520848103", "MaterialDescription": "RE Xba I rec., 250 kU", "RegisteredProductName": "Restriction endonuclease Xba I, recombinant, AOF", "GTIN": "", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "250 kU  (25 mL)", "PackSizeDescPIM360": "Not Available", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"RE Xba I rec., 250 kU\". Unit of measure is \"piece\"." } ] }, "Product2Products": { "Product2ProductReference": [ ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Language": "en", "Value": "The restriction enzyme Xba I linearizes template DNA plasmids to prepare for mRNA production.", "Country": "XG", "Code": "Positioning", "Name": "Positioning" }, { "Language": "en", "Value": "Xba I is supplied as a solution without a reaction buffer. The reaction buffer is available separately via material number 09109331103, material name: SuRE/Cut Buffer H, 500 ml.", "Country": "XG", "Code": "Product Description", "Name": "Product Description" }, { "Language": "en", "Value": "Restriction enzyme Xba I is used upstream of the in-vitro mRNA synthesis step during mRNA therapeutics and vaccine manufacturing. The enzyme linearizes plasmid vectors that serve as a template for the synthesis of mRNA via in-vitro transcription. It can also be used for other molecular applications such as cloning.", "Country": "XG", "Code": "Applications", "Name": "Applications" }, { "Language": "en", "Value": "

• Concentrate your efforts on mRNA drug design and clinical studies by relying on a proven enzyme in mRNA vaccine manufacturing.
• Enhance the safety of your mRNA therapeutic drug by using a biopharma quality enzyme that is animal-orgin-free (AOF) and antibiotic-free.

", "Country": "XG", "Code": "Benefits", "Name": "Benefits" }, { "Language": "en", "Value": "Xba I recognizes the sequence T/CTAGA and generates fragments with 5′-cohesive termini (Zain BS, Roberts RJ, 1977). The enzyme needs at least two nucleotides around the target sequence for cleavage.
CAS-Number: 81295.42.3
Optimum reaction temperature: +37°C", "Country": "XG", "Code": "Properties", "Name": "Properties" }, { "Language": "en", "Value": "Appearance: Clear, colorless solution
Volume Activity (λdam-minus, dcm-minus DNA as substrate): 10x10³ U/mL
Unit definition: One unit is the enzyme activity that completely cleaves 1 μg λdam– DNA in one hour at +37 °C in a total volume of 50 μL (1x) SuRE/Cut Buffer H.
Unspecific nucleases (up to 40 enzyme units using λ-DNA;16 h/+37°C; up to 40 enzyme units using pBR 322-DNA; 16 h/+37°C): not detectable
Heavy metals (Ni, Co, V): ≤ 10 ppm
Stability: At -25°C to -15°C within specification range for 18 months.", "Country": "XG", "Code": "Specification", "Name": "Specification" }, { "Language": "en", "Value": "09520848101: Will be supplied as \"RE Xba I rec., 50 kU\". Unit of measure is \"piece\".
09520848103: Will be supplied as \"RE Xba I rec., 250 kU\". Unit of measure is \"piece\".", "Country": "XG", "Code": "CB - Order Information", "Name": "CB - Order Information" } ] } } ] }