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  1. Cell lysis is accomplished by incubation of the sample in a special lysis buffer in the presence of Proteinase K.
  2. Subsequently, the DNA is precipitated and purified from salts, proteins, and other impurities by a washing step.
  3. In a final step, the DNA is dissolved in a low-salt buffer.
", "Name": "Principle" }, { "Language": "en", "Value": "
Vial / BottleCapLabelFunction / DescriptionContent
1orangeProteinase K
 
4 vials,
850 µl each
2brownLysis BufferContains guanidine thiocyanate, Tris buffer, and detergent.5 vials,
6 ml each
3greyPrecipitation ReagentTo precipitate nucleic acids.5 vials,
7 ml each
4whiteWashing BufferFor washing steps.6 vials,
9 ml each
5blueDissolution BufferTo dissolve the extracted nucleic acids.5 vials,
5 ml each
6whiteWater, DNA free10 vials,
1 ml each
7whitePoly(A)Lyophilized7 bottles,
2 mg each
8Reaction vials3 bags with 35 polypropylene tubes
(2 ml)
", "Name": "Content" }, { "Language": "en", "Value": "Each lot of the QC Sample Preparation Kit is function tested for the detection of residual host cell CHO DNA and mycoplasma with the MycoTOOL Mycoplasma Real-Time PCR Kit*.
All reagents of the QC Sample Preparation Kit are tested for the absence of DNA from mycoplasma, CHO cells, and E. coli.", "Name": "Quality Control" }, { "Language": "en", "Value": "Preparation of cell culture samples before PCR test detection of mycoplasma or residual DNA from CHO cells and from E. coli.", "Name": "Positioning" }, { "Language": "en", "Value": "The QC Sample Preparation Kit offers optimized extraction protocols to prepare sample for further PCR tests.

Detection of mycoplasma contamination
  • Using a PCR block cycler, refer to the method sheet (instructions for use) of the MycoTOOL Mycoplasma Detection Amplification Kit.
    - for testing of CHO or SP2/0 cells with 5 x 106 cells/mL (standard protocol)
    - for testing of CHO or SP2/0 samples up to 1 x 108 cells/mL (protocol for high cell density)
  • Using a real-time PCR instrument, refer to the method sheet (instructions for use) of the MycoTOOL Real-Time PCR Kit for testing of mammalian cell culture samples. This PCR kit contains a control plasmid (Recovery Control) that can be added to the sample material to prevent false negative results.
Detection of Host Cell DNA
  • Preparation of samples for testing 100 samples (per 100 μL) from fermentation process for detection of residual DNA from E. coli or CHO cells.
  • Refer to the method sheet (instructions for use) of the Residual DNA E. coli Kit, or of the Residual DNA CHO Kit. These PCR kits contain Standard DNA that can be added to the sample material to prevent false negative results and a workflow negative control as well.
", "Name": "Applications" }, { "Language": "en", "Value": "Unit of measure is “piece”.", "Name": "CB - Order Information" }, { "Language": "en", "Value": "Speed up by optimized extraction protocols to prepare samples for PCR tests and gain safety as the Kit does not include any Triton-X .", "Name": "Benefits" }, { "Language": "en", "Value": "The kit is designed to be used with the MycoTOOL Mycoplasma Detection Amplification Kit. Additionally it also can be used for the Residual DNA CHO Kit and Residual DNA E. coli Kit.", "Name": "Product Description" }, { "Language": "en", "Value": "It is designed for:
  • 26 extractions from mammalian cell culture samples (per 1 mL)
  • or 10 extractions from high density cell culture samples (per 1 mL)
  • or 100 extractions form fermentation process (per 100 μL).
Stability: At +15 to +25 °C within specification for 24 months.", "Name": "Specification" } ] } } ] }

QC Sample Preparation Kit

QC Sample Preparation Kit