{ "ProductData": { "RelatedLinks": "", "RegulatoryDisclaimer1": "For further processing only.", "RegulatoryDisclaimer2": "", "StainLocalization": [ "" ], "DisclaimerGroup2": null, "ProductNameGlobal": "Pyruvate Oxidase", "DisclaimerGroup1": "Other - Non IVD", "ControlTissue": [ "" ], "BrandName": "Pyruvate Oxidase", "LicenseDisclaimers": [ ], "Isotypes": "", "RegulatoryDisclaimer3": "", "ID": "3.1.1.4.29.4", "ProductNameAddition": "from E.coli overproducer, lyophilizate", "Species": [ "" ], "Clone": "" }, "ProductSpec": { "ProductSpecVariant": { "Chapters": [ { "Language": "English", "Value": "Appearance: Yellow lyophilizate
Solubility: Clear, yellowish solution in potassium phosphate buffer, 0.1 M, pH 6.5 (c=10 mg/mL)
Activity (+25°C, pyruvate, O₂, P_i): ≥1.5 U/mg lyophilizate
Specific activity: ≥3 U/mg protein
Protein (Biuret): ≥0.4 mg/mg lyophilizate
Contaminants (expressed as percentage of Pyruvate Oxidase activity):
ATPase: No limit
Glucose oxidase: ≤0.001
Aspartate aminotransferase (AST/GOT): ≤0.01
Alanine aminotransferase (ALT/GPT): ≤0.01
apo-Alanine aminotransferase (apo-ALT/apo-GPT) : ≤0.005
Impurities, total: ≤0.02
Lactate oxidase: ≤0.002
α-Ketoglutarate oxidase: ≤0.02
''NADH oxidase'': ≤0.02
Stability: At -15 to -25°C within specification range for 12 months. Store dry.", "Name": "CB - Specification" }, { "Language": "English", "Value": "Use Pyruvate Oxidase in a variety of diagnostic tests, such as for the determination of pyruvate, lactate or aminotransferases.", "Name": "Applications" }, { "Language": "English", "Value": "Will be supplied as \"Pyruvate Oxidase Recombinant (E. coli)\". Unit of measure is \"kU\".", "Name": "CB - Order Information" }, { "Language": "English", "Value": "Recombinant oxidoreductase that catalyzes the interconversion of pyruvate to acetyl phosphate.", "Name": "Positioning" }, { "Language": "English", "Value": "Appearance: Yellow lyophilizate
Solubility: Clear, yellowish solution in potassium phosphate buffer, 0.1 M, pH 6.5 (c=10 mg/mL)
Activity (+25°C, pyruvate, O₂, P_i): ≥1.5 U/mg lyophilizate
Specific activity: ≥3 U/mg protein
Protein (Biuret): ≥0.4 mg/mg lyophilizate
Contaminants (expressed as percentage of Pyruvate Oxidase activity):
ATPase: No limit
Glucose oxidase: ≤0.001
Aspartate aminotransferase (AST/GOT): ≤0.01
Alanine aminotransferase (ALT/GPT): ≤0.01
apo-Alanine aminotransferase (apo-ALT/apo-GPT) : ≤0.005
Impurities, total: ≤0.02
Lactate oxidase: ≤0.002
α-Ketoglutarate oxidase: ≤0.02
''NADH oxidase'': ≤0.02
Stability: At -15 to -25°C within specification range for 12 months. Store dry.", "Name": "Specification" } ] } }, "Product2Taxomony": { "Product2TaxomonyReferences": [ { "StructureSystemName": "OWP Product Solutions", "NodeName": "CustomBiotech", "StructureSystemID": null, "NodeID": "520" }, { "StructureSystemName": "Product Grouping", "NodeName": "CustomBiotech->Enzymes", "StructureSystemID": null, "NodeID": "02-0014" }, { "StructureSystemName": "OWP Product Types", "NodeName": "Raw Materials and Reagents->Oxidases", "StructureSystemID": null, "NodeID": "70-040-00" }, { "StructureSystemName": "OWP Organization", "NodeName": "Roche Diagnostics Solution (RDS)->Customer Areas->Core lab->CustomBiotech and LifeScience", "StructureSystemID": null, "NodeID": "01-01-01-07-00" }, { "StructureSystemName": "Applications", "NodeName": "Carbohydrate Testing", "StructureSystemID": null, "NodeID": "15-00-00" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "11418912103", "MaterialDescription": "Pyruvate Oxidase Recombinant (E. coli)", "RegisteredProductName": "", "GTIN": "", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "custom fill", "PackSizeDescPIM360": "", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"Pyruvate Oxidase Recombinant (E. coli)\". Unit of measure is \"kU\"." } ] }, "Product2Products": { "Product2ProductReference": [ ] } }