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"Health_Topics", "NameofStructureSystem": "Health Topics", "StructureNodeID": "99-00-00", "StructureGroupPath": "NOT APPLICABLE", "StructureGroupName": "NOT APPLICABLE" } ] } ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Language": "en", "Value": "Keep at +2 to +10°C for on-board use.
After first use on the instrument, the reagent cassette is stable for up to 4 weeks on board.", "Name": "Storage Conditions (Working Solution)" }, { "Language": "en", "Value": "Store the reagent at +2 to +8°C.", "Name": "Storage Conditions (Product)" }, { "Language": "en", "Value": "PYRB is the standard protocol for pyruvate determination. If the pyruvate concentration of a sample is higher than 696 mg/L (8 mmol/L), then protocol PYRD should be used (same protocol with pre-set 1:10 sample predilution).
Results higher than the valid measuring range will be flagged and a rerun with an automatic 1:10 predilution will be recommended by the software.


Cedex Bio test definition:
  • Measuring mode                          Absorbance
  • Abs. calculation mode                 Endpoint
  • Reaction mode                             R1-R2-S
  • Reaction direction                        Decrease
  • Wavelength A/B                            340/378 nm
  • Calc. first/last                                6/30
  • Unit                                                  mmol/L; mg/L

Pipetting parameters:
  • R1                                                    70 μL + 64 μL Diluent (H20)
  • R2                                                    20 μL
  • Sample                                           6 μL + 10 μL Diluent (H2O)
  • Total volume                                  170 μL

Calibration:
  • Calibrator                                      Calibrator D Bio (CAL D, 794)
  • Calibration mode                        Linear regression
  • Calibration interval                      56 days, and if recalibration is required due to QC results

Quality control:
  • Control D Level 1 Bio (COND1, 307)
  • Control D Level 2 Bio (COND2, 308)
  • Control D Level 3 Bio (COND3, 309)

Use the recommended control material. Other suitable control material can be used in addition.
Control intervals and acceptance limits should be adapted to each laboratory's individual requirements.
If values do not fall into the defined limits, corrective measures and recalibration are required.", "Name": "Protocols - Help Corner" }, { "Language": "en", "Value": "Unit of measure is \"piece\".", "Name": "CB - Order Information" }, { "Language": "en", "Value": "
Analyzer and accessories
  • Cedex Bio Analyzer*, including accessories and disposables
  • Standard laboratory equipment
Calibrators and Controls
  • Calibrator D Bio*
  • Control D Level 1 Bio*
  • Control D Level 2 Bio*
  • Control D Level 3 Bio*
", "Name": "Additional Equipment and Reagent Required" }, { "Language": "en", "Value": "Kit for determination of pyruvate.", "Name": "Product Description" }, { "Language": "en", "Value": "Pyruvate concentration can be determined in following ranges:

  • PYRB: 17.4 to 696 mg/L (0.2 to 8 mmol/L)
  • PYRD: 174 to 6960 mg/L (2.0 to 80 mmol/L),

up to 69.6 g/L (0.8 mol/L) with postdilution

Conversion Factors
Conversion factors for pyruvate concentration:

  • 1 mmol/L = 87.06 mg/L
  • 1 g/L = 11.49 mmol/L

Traceability

This method has been standardized against titration with HClO4.

Precision
Representative performance data on Cedex Bio Analyzers are shown. Results obtained in individual laboratories may differ.
Precision was determined in samples of three concentration levels. Coefficients of variation (CV) were calculated for in-run precision (n=21) and inter-run precision (on 10 days).

  Level 1 Level 2 Level 3
Mean 0.52 mmol/L
(45.3 mg/L)
          3.1 mmol/L
          (270 mg/L)
       6.2 mmol/L
       (540 mg/L)
CV in-run 1.2%           1.6%        1.7%
CV inter-run 2.2%           1.9 %        1.1 %
", "Name": "Background Information - Help Corner" }, { "Language": "en", "Value": "
VialCapContentComposition
1 PYRB R1white4 vials,
50 tests each
Reagent 1 (R1): TRIS, 230 mmol/L; sodium chloride, 80 mmol/L; lactate dehydrogenase, approximately 4,000 U/L; stabilizer; preservative; pH 7.6.
2 PYRB R2black4 vials,
500 tests each
Reagent 2 (R2): Sodium bicarbonate, 20 mmol/L; NADH, 3.2 mmol/L; preservative; pH 10.2.
", "Name": "Content" }, { "Language": "en", "Value": "Pyruvate is an important source of carbon and energy in cell cultures. It is generated as an intermediate in the glycolysis pathway by the cells using glucose. Control of the pyruvate concentration in the culture by additional feeding of pyruvate enables optimization of cell growth and product formation. For control of culture media and for monitoring in a culture process, the pyruvate concentration can be determined on a Cedex Bio or Cedex Bio HT Analyzer with high reliability and convenience.", "Name": "Applications" }, { "Language": "en", "Value": "Method: Enzymatic photometric assay using lactate dehydrogenase.
Recovery: ±15%", "Name": "Specification" } ] } } ] }

Pyruvate Bio

Test kit for the Cedex Bio Analyzer

Pyruvate Bio

Overview

Ordering

Technical documents

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