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Pyrophosphatase is produced in antibiotic-free and animal-free conditions
QC-release testing includes extended impurity testing e.g. host-cell DNA, host cell proteins, endotoxin, bioburden, heavy metals
",
"Name": "Benefits"
},
{
"Language": "en",
"Value": "Manufactured under GMP (Good Manufacturing Practice) regulations. No animal-derived material is added in fermentation, purification and final formulation of this enzyme.",
"Name": "Quality Control"
},
{
"Language": "en",
"Value": "Pyrophosphatase hydrolyzes inorganic pyrophosphate generated during in vitro transcription reactions. By removing unwanted pyrophosphate, the equilibrium is shifted to the product side increasing RNA yield. Cofactor: Mg2+ is required for enzymatic activity",
"Name": "Applications"
},
{
"Language": "en",
"Value": "Pyrophosphatase is an enzyme, which hydrolyzes inorganic pyrophosphate.",
"Name": "Positioning"
},
{
"Language": "en",
"Value": "Pyrophosphatase is expressed with an N-terminal His-Tag. Molecular weight: 63 kD",
"Name": "Properties"
},
{
"Language": "en",
"Value": "Appearance: Clear, colorless to slightly yellowish solution Volume activity: ≥ 600 U/mL Protein (abs. 280nm): 1.8-2.2 mg/mL Purity (SDS-PAGE): ≥ 95 % Unspecific endonucleases (up to 300 ng enzyme using MWM III-DNA; 16 h/37°C): not detectable Nicking activity (up to 300 ng enzyme using pBR 322-DNA) 16 h/37°C): not detectable RNase activity (up to 300 ng enzyme using MS II-RNA; 1 h/37°C): not detectable Proteases (50 μg/15 min/37°C): not detectable Exonucleases (using 1 μg): not detectable Endotoxins: ≤ 10 EU/mL Bioburden: ≤ 10 CFU/mL E. coli host cell DNA: ≤ 100 pg/mg Protein E. coli host cell protein: ≤ 50 ppm Heavy metals (Ni, Co, V): ≤ 10 ppm Stability: At -15 to -25°C within specification range for 18 months.",
"Name": "Specification"
}
]
}
}
]
}
