{ "ProductData": { "RelatedLinks": "", "RegulatoryDisclaimer1": "For further processing only.", "RegulatoryDisclaimer2": "", "StainLocalization": [ "" ], "DisclaimerGroup2": null, "ProductNameGlobal": "Pyrophosphatase recombinant GMP Grade", "DisclaimerGroup1": "Other - Non IVD", "ControlTissue": [ "" ], "BrandName": "Pyrophosphatase, recombinant, GMP Grade", "LicenseDisclaimers": [ ], "Isotypes": "", "RegulatoryDisclaimer3": "", "ID": "3.1.5.4.5.2", "ProductNameAddition": "from yeast, expressed in E. coli, solution", "Species": [ "" ], "Clone": "" }, "ProductSpec": { "ProductSpecVariant": { "Chapters": [ { "Language": "English", "Value": "Appearance: Clear, colorless to slightly yellowish solution
Volume activity: ≥ 600 U/mL
Protein (abs. 280nm): 1.8-2.2 mg/mL
Purity (SDS-PAGE): ≥ 95 %
Unspecific endonucleases (up to 300 ng enzyme using MWM III-DNA; 16 h/37°C): not detectable
Nicking activity (up to 300 ng enzyme using pBR 322-DNA) 16 h/37°C): not detectable
RNase activity (up to 300 ng enzyme using MS II-RNA; 1 h/37°C): not detectable
Proteases (50 μg/15 min/37°C): not detectable
Exonucleases (using 1 μg): not detectable
Endotoxins: ≤ 10 EU/mL
Bioburden: ≤ 10 CFU/mL
E. coli host cell DNA: ≤ 100 pg/mg Protein
E. coli host cell protein: ≤ 50 ppm
Heavy metals (Ni, Co, V): ≤ 10 ppm
Stability: At -15 to -25°C within specification range for 18 months.", "Name": "CB - Specification" }, { "Language": "English", "Value": "Will be supplied as \"Pyrophosphatase, rec., GMP Grade, 20 ml\". Unit of measure is \"piece\". ", "Name": "CB - Order Information" }, { "Language": "English", "Value": "Pyrophosphatase is expressed with an N-terminal His-Tag.
Molecular weight: 63 kD", "Name": "CB - Properties" }, { "Language": "English", "Value": "Use fit-for-purpose Pyrophosphatase for manufacturing of therapeutic mRNA:

• Pyrophosphatase is produced in antibiotic-free and animal-free conditions
• QC-release testing includes extended impurity testing e.g. host-cell DNA, host cell proteins, endotoxin, bioburden, heavy metals

", "Name": "Benefits" }, { "Language": "English", "Value": "Manufactured under GMP (Good Manufacturing Practice) regulations. No animal-derived material is added in fermentation, purification and final formulation of this enzyme.", "Name": "Quality Control" }, { "Language": "English", "Value": "Pyrophosphatase hydrolyzes inorganic pyrophosphate generated during in vitro transcription reactions. By removing unwanted pyrophosphate, the equilibrium is shifted to the product side increasing RNA yield.
Cofactor: Mg²⁺ is required for enzymatic activity", "Name": "Applications" }, { "Language": "English", "Value": "Pyrophosphatase is an enzyme, which hydrolyzes inorganic pyrophosphate.", "Name": "Positioning" }, { "Language": "English", "Value": "Pyrophosphatase is expressed with an N-terminal His-Tag.
Molecular weight: 63 kD", "Name": "Properties" }, { "Language": "English", "Value": "Appearance: Clear, colorless to slightly yellowish solution
Volume activity: ≥ 600 U/mL
Protein (abs. 280nm): 1.8-2.2 mg/mL
Purity (SDS-PAGE): ≥ 95 %
Unspecific endonucleases (up to 300 ng enzyme using MWM III-DNA; 16 h/37°C): not detectable
Nicking activity (up to 300 ng enzyme using pBR 322-DNA) 16 h/37°C): not detectable
RNase activity (up to 300 ng enzyme using MS II-RNA; 1 h/37°C): not detectable
Proteases (50 μg/15 min/37°C): not detectable
Exonucleases (using 1 μg): not detectable
Endotoxins: ≤ 10 EU/mL
Bioburden: ≤ 10 CFU/mL
E. coli host cell DNA: ≤ 100 pg/mg Protein
E. coli host cell protein: ≤ 50 ppm
Heavy metals (Ni, Co, V): ≤ 10 ppm
Stability: At -15 to -25°C within specification range for 18 months.", "Name": "Specification" } ] } }, "Product2Taxomony": { "Product2TaxomonyReferences": [ { "StructureSystemName": "OWP Product Solutions", "NodeName": "CustomBiotech", "StructureSystemID": null, "NodeID": "520" }, { "StructureSystemName": "Product Grouping", "NodeName": "CustomBiotech->mRNA Reagents", "StructureSystemID": null, "NodeID": "02-0062" }, { "StructureSystemName": "OWP Product Types", "NodeName": "Raw Materials and Reagents->Phosphatases", "StructureSystemID": null, "NodeID": "70-041-00" }, { "StructureSystemName": "Product Grouping", "NodeName": "CustomBiotech->Cell & Gene Therapy", "StructureSystemID": null, "NodeID": "02-0133" }, { "StructureSystemName": "OWP Organization", "NodeName": "Roche Diagnostics Solution (RDS)->Customer Areas->Core lab->CustomBiotech and LifeScience", "StructureSystemID": null, "NodeID": "01-01-01-07-00" }, { "StructureSystemName": "Product Grouping", "NodeName": "CustomBiotech->Enzymes", "StructureSystemID": null, "NodeID": "02-0014" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "08140677103", "MaterialDescription": "Pyrophosphatase, rec., GMP Grade, 20 ml", "RegisteredProductName": "", "GTIN": "", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "20 mL", "PackSizeDescPIM360": "", "MaterialAnnotation": "", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"Pyrophosphatase, rec., GMP Grade, 20 ml\". Unit of measure is \"piece\"." } ] }, "Product2Products": { "Product2ProductReference": [ ] } }