{ "ProductData": { "RelatedLinks": "", "RegulatoryDisclaimer1": "For further processing only.", "RegulatoryDisclaimer2": "", "StainLocalization": [ "" ], "DisclaimerGroup2": null, "ProductNameGlobal": "Proteinase K recombinant PCR Grade lyophilizate", "DisclaimerGroup1": "Other - Non IVD", "ControlTissue": [ "" ], "BrandName": "Proteinase K, recombinant, PCR Grade", "LicenseDisclaimers": [ ], "Isotypes": "", "RegulatoryDisclaimer3": "", "ID": "3.6.14.8.1.7", "ProductNameAddition": "from Titrirachium album, expressed in Pichia pastoris, lyophilizate", "Species": [ "" ], "Clone": "" }, "ProductSpec": { "ProductSpecVariant": { "Chapters": [ { "Language": "English", "Value": "Recombinant Proteinase K in PCR Grade quality is a universal tool for nucleic acid template preparation.", "Name": "Positioning" }, { "Language": "English", "Value": "

• Maximize the yield of target nucleic acids.
  Proteinase K is rigorously tested for the absence of nucleases.
• Experience stability and flexibility.
  The lyophilizate is very stable and can be stored at room temperature for at least 12 months. A broad range of fill volumes from 25 mg to 5 g per vial is available. Lyophilizate dissolved in water is stable for at least 60 days when stored at +2 to +4°C.

", "Name": "Benefits" }, { "Language": "English", "Value": "Proteinase K, recombinant, PCR Grade, digests native proteins very efficiently. This enzyme can be used to rapidly inactivate endogenous RNases and DNases during nucleic acid isolation. Proteinase K is particularly suited for the isolation of native RNA and DNA from tissues and cell lines.
The enzyme promotes cell lysis by activating a bacterial autolytic factor. Proteinase K is also used for:

• Analysis of membrane structures by modifying proteins and glycoproteins on cell surfaces
• Removal of cellular debris during the preparation of colony lifts
• Treatment of tissue sections to ensure efficient probe infiltration during in situ hybridization

", "Name": "Applications" }, { "Language": "English", "Value": "Proteinase K, originally isolated from the mold Tritirachium album, is a recombinant enzyme expressed in Pichia pastoris. It is a highly active, subtilisin-related serine endopeptidases that does not exhibit any pronounced cleavage specificity. Thus, Proteinase K, recombinant, PCR Grade, is a universal tool for template preparation. Amino acid sequence and molecular structure of the recombinant enzyme and the native protease are identical. However, the production process of the recombinant Proteinase K guarantees an enzyme of outstanding reliability and purity meeting all requirements of diagnostics' manufacturers.
Special emphasis has been placed on a low DNA-content of the enzyme preparation, making Proteinase K, recombinant, PCR Grade, ideally suited for isolating PCR and RT-PCR templates.", "Name": "Product Description" }, { "Language": "English", "Value": "03450376103: Will be supplied as \"Protein. K, rec PCR Grade Lyo. MPB 25 mg\". Unit of measure is \"piece\".
03508811103: Will be supplied as \"Protein. K, rec PCR Grade Lyo. MPB 50 mg\". Unit of measure is \"piece\".
03508838103: Will be supplied as \"Protein. K, rec PCR Grade Lyo. MPB 100 mg\". Unit of measure is \"piece\".
03450384103: Will be supplied as \"Protein. K, rec PCR Grade Lyo. MPB 250 mg\". Unit of measure is \"piece\".
05963117103: Will be supplied as \"Proteinase K, rec., PCR Grade, Lyo 5 g\". Unit of measure is \"piece\".", "Name": "CB - Order Information" }, { "Language": "English", "Value": "Appearance: White lyophilizate
Solubility: Clear, colorless solution in water (c=20 mg/mL)
Volume activity (+37°C, hemoglobin): ≥24 U/mg lyophilizate
Specific activity (+37°C, hemoglobin): ≥30 U/mg protein
Unit definition (hemoglobin): One unit is the enzyme activity which releases folin positive amino acids and peptides equivalent to 1 μmol of tyrosine in 1 minute under the test conditions.
Volume activity (+25°C, Chromozym): ≥2 U/mg lyophilizate
Specific activity (+25°C, Chromozym): ≥2.5 U/mg protein
Unit definition (Chromozym): One unit is the enzyme activity which cleaves 8 mmol Chromozym TRY in 1 minute at +25°C.
Unspecific endonucleases (MWM III DNA): Not detectable in up to 200 μg after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 200 μg after 16 hours incubation at +37°C.
Ribonucleases (MS2 RNA): Not detectable in up to 40 μg after 16 hours incubation at +37°C.
DNA (Threshold^®): ≤10 pg/mg enzyme
Bioburden: ≤125 CFU/g
Stability: At +2 to +8°C within specification range for 18 months.", "Name": "CB - Specification" }, { "Language": "English", "Value": "Nomenclature: Proteinase K
Molecular weight: 28.8 kD
pH optimum: 7.5-10.5
Inhibitors: Proteinase K is inhibited by diisopropyl fluorophosphate and phenylmethylsulfonyl fluoride (PMSF) and is also totally inactivated by mercuric ions. Pefabloc^® SC and Pefabloc^® PLUS are specific, irreversible and nontoxic inhibitors.
Specificity: Proteinase K is one of the most active endopeptidases known and does not exhibit any pronounced cleavage specificity. Activity can be stimulated by addition of denaturing agents (SDS and urea).", "Name": "CB - Properties" }, { "Language": "English", "Value": "Nomenclature: Proteinase K
Molecular weight: 28.8 kD
pH optimum: 7.5-10.5
Inhibitors: Proteinase K is inhibited by diisopropyl fluorophosphate and phenylmethylsulfonyl fluoride (PMSF) and is also totally inactivated by mercuric ions. Pefabloc^® SC and Pefabloc^® PLUS are specific, irreversible and nontoxic inhibitors.
Specificity: Proteinase K is one of the most active endopeptidases known and does not exhibit any pronounced cleavage specificity. Activity can be stimulated by addition of denaturing agents (SDS and urea).", "Name": "Properties" }, { "Language": "English", "Value": "Appearance: White lyophilizate
Solubility: Clear, colorless solution in water (c=20 mg/mL)
Volume activity (+37°C, hemoglobin): ≥24 U/mg lyophilizate
Specific activity (+37°C, hemoglobin): ≥30 U/mg protein
Unit definition (hemoglobin): One unit is the enzyme activity which releases folin positive amino acids and peptides equivalent to 1 μmol of tyrosine in 1 minute under the test conditions.
Volume activity (+25°C, Chromozym): ≥2 U/mg lyophilizate
Specific activity (+25°C, Chromozym): ≥2.5 U/mg protein
Unit definition (Chromozym): One unit is the enzyme activity which cleaves 8 mmol Chromozym TRY in 1 minute at +25°C.
Unspecific endonucleases (MWM III DNA): Not detectable in up to 200 μg after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 200 μg after 16 hours incubation at +37°C.
Ribonucleases (MS2 RNA): Not detectable in up to 40 μg after 16 hours incubation at +37°C.
DNA (Threshold^®): ≤10 pg/mg enzyme
Bioburden: ≤125 CFU/g
Stability: At +2 to +8°C within specification range for 18 months.", "Name": "Specification" } ] } }, "Product2Taxomony": { "Product2TaxomonyReferences": [ { "StructureSystemName": "OWP Product Solutions", "NodeName": "CustomBiotech", "StructureSystemID": null, "NodeID": "520" }, { "StructureSystemName": "Product Grouping", "NodeName": "CustomBiotech->Molecular Raw Materials", "StructureSystemID": null, "NodeID": "02-0136" }, { "StructureSystemName": "OWP Product Types", "NodeName": "Raw Materials and Reagents->Proteases", "StructureSystemID": null, "NodeID": "70-044-00" }, { "StructureSystemName": "Product Grouping", "NodeName": "CustomBiotech->Enzymes", "StructureSystemID": null, "NodeID": "02-0014" }, { "StructureSystemName": "OWP Techniques", "NodeName": "Nucleic acid extraction and purification", "StructureSystemID": null, "NodeID": "241-00" }, { "StructureSystemName": "OWP Techniques", "NodeName": "Sample preparation", "StructureSystemID": null, "NodeID": "130-00" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "03508838103", "MaterialDescription": "Protein._K, rec_PCR_Grade_Lyo. MPB 100mg", "RegisteredProductName": "", "GTIN": "", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "custom fill", "PackSizeDescPIM360": "", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"Protein. K, rec PCR Grade Lyo. MPB 100 mg\". Unit of measure is \"piece\"" }, { "MaterialNum": "03508811103", "MaterialDescription": "Protein._K, rec_PCR_Grade_Lyo. MPB 25mg", "RegisteredProductName": "", "GTIN": "", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "custom fill", "PackSizeDescPIM360": "", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"Protein. K, rec PCR Grade Lyo. MPB 25 mg\". Unit of measure is \"piece\"." }, { "MaterialNum": "03450376103", "MaterialDescription": "Protein._K, rec_PCR_Grade_Lyo. MPB 50mg", "RegisteredProductName": "", "GTIN": "", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "custom fill", "PackSizeDescPIM360": "", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"Protein. K, rec PCR Grade Lyo. MPB 50 mg\". Unit of measure is \"piece\"." }, { "MaterialNum": "03450384103", "MaterialDescription": "Protein._K, rec_PCR_Grade_Lyo. MPB 250mg", "RegisteredProductName": "", "GTIN": "", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "custom fill", "PackSizeDescPIM360": "", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"Protein. K, rec PCR Grade Lyo. MPB 250 mg\". Unit of measure is \"piece\"." }, { "MaterialNum": "05963117103", "MaterialDescription": "Proteinase K rec. PCR Grade Lyo 5 g", "RegisteredProductName": "", "GTIN": "07613336132883", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "custom fill", "PackSizeDescPIM360": "", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"Proteinase K, rec., PCR Grade, Lyo 5 g\". Unit of measure is \"piece\"." } ] }, "Product2Products": { "Product2ProductReference": [ ] } }