{ "ProductData": { "RelatedLinks": "", "RegulatoryDisclaimer1": "For further processing only.", "RegulatoryDisclaimer2": "", "StainLocalization": [ "" ], "ProductType": "Others", "DisclaimerGroup2": null, "ProductNameGlobal": "Proteinase K recombinant PCR Grade lyophilizate", "DisclaimerGroup1": "Non-IVD", "ControlTissue": [ "" ], "BrandName": "Proteinase K, recombinant, PCR Grade", "LicenseDisclaimers": [ ], "Isotypes": "", "RegulatoryDisclaimer3": "", "ID": "3.6.14.8.1.7", "ProductNameAddition": "from Titrirachium album, expressed in Pichia pastoris, lyophilizate", "Species": [ "" ], "Clone": "" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/CB_IMAGE.png", "ImageType": "Image main" }, "Product2Taxomony": { "Product2TaxomonyReferences": [ { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "Molecular Raw Materials", "NodeID": "02-0136" }, { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "NodeName": "Proteases", "NodeID": "70-044-00" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "Enzymes", "NodeID": "02-0014" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "NodeName": "Nucleic Acid Extraction and Purification", "NodeID": "241-00" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "NodeName": "Sample Preparation", "NodeID": "130-00" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "NodeName": "CustomBiotech", "NodeID": "520" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeName": "Manufacturing", "NodeID": "180-00" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "NodeName": "NOT APPLICABLE", "NodeID": "999" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "Biologics", "NodeID": "02-0008" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "03508838103", "MaterialDescription": "Protein._K, rec_PCR_Grade_Lyo. MPB 100mg", "RegisteredProductName": "Proteinase K, recombinant, PCR Grade", "GTIN": "", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "100 mg, unlabeled vial", "PackSizeDescPIM360": "", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"Protein. K, rec PCR Grade Lyo. MPB 100 mg\". Unit of measure is \"piece\". Minimum order quantity is 100 pcs." }, { "MaterialNum": "03508811103", "MaterialDescription": "Protein._K, rec_PCR_Grade_Lyo. MPB 25mg", "RegisteredProductName": "Proteinase K, recombinant, PCR Grade", "GTIN": "", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "25 mg, unlabeled vial", "PackSizeDescPIM360": "", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"Protein. K, rec PCR Grade Lyo. MPB 25 mg\". Unit of measure is \"piece\". Minimum order quantity is 300 pcs." }, { "MaterialNum": "03450376103", "MaterialDescription": "Protein._K, rec_PCR_Grade_Lyo. MPB 50mg", "RegisteredProductName": "Proteinase K, recombinant, PCR Grade", "GTIN": "", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "50 mg, unlabeled vial", "PackSizeDescPIM360": "", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"Protein. K, rec PCR Grade Lyo. MPB 50 mg\". Unit of measure is \"piece\". Minimum order quantity is 45 pcs." }, { "MaterialNum": "03450384103", "MaterialDescription": "Protein._K, rec_PCR_Grade_Lyo. MPB 250mg", "RegisteredProductName": "Proteinase K, recombinant, PCR Grade", "GTIN": "", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "250 mg, unlabeled vial", "PackSizeDescPIM360": "", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"Protein. K, rec PCR Grade Lyo. MPB 250 mg\". Unit of measure is \"piece\". Minimum order quantity is 25 pcs." }, { "MaterialNum": "05963117103", "MaterialDescription": "Proteinase K rec. PCR Grade Lyo 5 g", "RegisteredProductName": "Proteinase K, recombinant, PCR Grade", "GTIN": "07613336132883", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "5 g", "PackSizeDescPIM360": "", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"Proteinase K, rec., PCR Grade, Lyo 5 g\". Unit of measure is \"piece\". Minimum order quantity is 2 pcs." } ] }, "Product2Products": { "Product2ProductReference": [ ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Language": "en", "Value": "Recombinant Proteinase K in PCR Grade quality is a universal tool for nucleic acid template preparation.", "Name": "Positioning" }, { "Language": "en", "Value": "

• Maximize the yield of target nucleic acids.
  Proteinase K is rigorously tested for the absence of nucleases.
• Experience stability and flexibility.
  The lyophilizate is very stable and can be stored at room temperature for at least 12 months. A broad range of fill volumes from 25 mg to 5 g per vial is available. Lyophilizate dissolved in water is stable for at least 60 days when stored at +2 to +4°C.

", "Name": "Benefits" }, { "Language": "en", "Value": "Proteinase K, recombinant, PCR Grade, digests proteins very efficiently. It can be used to rapidly inactivate endogenous RNases and DNases during nucleic acid isolation or remove enzymes that have been used during (m)RNA synthesis by in vitro transcription, as in the manufacture of mRNA vaccines.
Proteinase K is also used to:

• Isolate native RNA and DNA from tissues and cell lines by promoting cell lysis
• Analyze membrane structures by modifying proteins and glycoproteins on cell surfaces
• Remove cellular debris during the preparation of colony lifts
• Treat tissue sections to ensure efficient probe infiltration during in situ hybridization

", "Name": "Applications" }, { "Language": "en", "Value": "Proteinase K, originally isolated from the mold Tritirachium album, is a recombinant enzyme expressed in Pichia pastoris. It is a highly active, subtilisin-related serine endopeptidases that does not exhibit any pronounced cleavage specificity. Thus, Proteinase K, recombinant, PCR Grade, is a universal tool for template preparation. Amino acid sequence and molecular structure of the recombinant enzyme and the native protease are identical. However, the production process of the recombinant Proteinase K guarantees an enzyme of outstanding reliability and purity meeting all requirements of diagnostics' manufacturers.
Special emphasis has been placed on a low DNA-content of the enzyme preparation, making Proteinase K, recombinant, PCR Grade, ideally suited for isolating PCR and RT-PCR templates.", "Name": "Product Description" }, { "Language": "en", "Value": "03450376103: Will be supplied as \"Protein. K, rec PCR Grade Lyo. MPB 50 mg\". Unit of measure is \"piece\". Minimum order quantity is 125 pcs.
03508811103: Will be supplied as \"Protein. K, rec PCR Grade Lyo. MPB 25 mg\". Unit of measure is \"piece\". Minimum order quantity is 200 pcs.
03508838103: Will be supplied as \"Protein. K, rec PCR Grade Lyo. MPB 100 mg\". Unit of measure is \"piece\". Minimum order quantity is 75 pcs.
03450384103: Will be supplied as \"Protein. K, rec PCR Grade Lyo. MPB 250 mg\". Unit of measure is \"piece\". Minimum order quantity is 50 pcs.
05963117103: Will be supplied as \"Proteinase K, rec., PCR Grade, Lyo 5 g\". Unit of measure is \"piece\". Minimum order quantity is 2 pcs.", "Name": "CB - Order Information" }, { "Language": "en", "Value": "Nomenclature: Proteinase K
Molecular weight: 28.8 kD
pH optimum: 7.5-10.5
Inhibitors: Proteinase K is inhibited by diisopropyl fluorophosphate and phenylmethylsulfonyl fluoride (PMSF) and is also totally inactivated by mercuric ions. Pefabloc^® SC and Pefabloc^® PLUS are specific, irreversible and nontoxic inhibitors.
Specificity: Proteinase K is one of the most active endopeptidases known and does not exhibit any pronounced cleavage specificity. Activity can be stimulated by addition of denaturing agents (SDS and urea).", "Name": "Properties" }, { "Language": "en", "Value": "Appearance: White lyophilizate
Solubility: Clear, colorless solution in water (c=20 mg/mL)
Volume activity (+37°C, hemoglobin): ≥24 U/mg lyophilizate
Specific activity (+37°C, hemoglobin): ≥30 U/mg protein
Unit definition (hemoglobin): One unit is the enzyme activity which releases folin positive amino acids and peptides equivalent to 1 μmol of tyrosine in 1 minute under the test conditions.
Volume activity (+25°C, Chromozym): ≥2 U/mg lyophilizate
Specific activity (+25°C, Chromozym): ≥2.5 U/mg protein
Unit definition (Chromozym): One unit is the enzyme activity which cleaves 8 mmol Chromozym TRY in 1 minute at +25°C.
Unspecific endonucleases (MWM III DNA): Not detectable in up to 200 μg after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 200 μg after 16 hours incubation at +37°C.
Ribonucleases (MS2 RNA): Not detectable in up to 40 μg after 16 hours incubation at +37°C.
DNA (Threshold^®): ≤10 pg/mg enzyme
Bioburden: ≤125 CFU/g
Stability: At +2 to +8°C within specification range for 18 months.", "Name": "Specification" } ] } } ] }