For further processing only. Others Proteinase K recombinant PCR Grade lyo Proteinase K, rec., PCR Grade, lyo. 3.6.14.8.1.7 from Titrirachium album, expressed in Pichia pastoris, lyophilizate 05 963 117 103 5 963 117 103 05963117103 5963117103 05963117103 Proteinase K rec. PCR Grade Lyo 5 g Proteinase K, recombinant, PCR Grade 07613336132883 Reagents, kits 5 g Not Available CustomBiotech product. Please contact your local representative. Will be supplied as "Proteinase K, rec., PCR Grade, Lyo 5 g". Unit of measure is "piece". Minimum order quantity is 2 pcs. Proteinase K, rec., PCR Grade, lyophilizate is a versatile enzyme commonly employed to eliminate protein contamination from DNA and (m)RNA preparations. en Proteinase K, recombinant, PCR Grade, digests proteins very efficiently. It can be used to rapidly inactivate endogenous RNases and DNases during nucleic acid isolation or remove enzymes that have been used during (m)RNA synthesis by in vitro transcription, as in the manufacture of mRNA vaccines.Proteinase K is also used to:Isolate native RNA and DNA from tissues and cell lines by promoting cell lysisAnalyze membrane structures by modifying proteins and glycoproteins on cell surfacesRemove cellular debris during the preparation of colony liftsTreat tissue sections to ensure efficient probe infiltration during in situ hybridization en Appearance: White lyophilizateSolubility: Clear, colorless solution in water (c=20 mg/mL)Volume activity (+37°C, hemoglobin): ≥24 U/mg lyophilizateSpecific activity (+37°C, hemoglobin): ≥30 U/mg proteinUnit definition (hemoglobin): One unit is the enzyme activity which releases folin positive amino acids and peptides equivalent to 1 μmol of tyrosine in 1 minute under the test conditions.Volume activity (+25°C, Chromozym): ≥2 U/mg lyophilizateSpecific activity (+25°C, Chromozym): ≥2.5 U/mg proteinUnit definition (Chromozym): One unit is the enzyme activity which cleaves 8 mmol Chromozym TRY in 1 minute at +25°C.Unspecific endonucleases (MWM III DNA): Not detectable in up to 200 μg after 16 hours incubation at +37°C.Nicking activity (pBR322 DNA): Not detectable in up to 200 μg after 16 hours incubation at +37°C.Ribonucleases (MS2 RNA): Not detectable in up to 40 μg after 16 hours incubation at +37°C.DNA (Threshold®): ≤10 pg/mg enzymeBioburden: ≤125 CFU/gStability: At +2 to +8°C within specification range for 18 months. en

Proteinase K - a universal tool for nucleic acid template preparation

Proteinase K, originally isolated from the mold Tritirachium album, is a recombinant enzyme expressed in Pichia pastoris. It is a highly active, subtilisin-related serine endopeptidases that does not exhibit any pronounced cleavage specificity. Thus, Proteinase K, recombinant, PCR Grade, is a universal tool for template preparation. Amino acid sequence and molecular structure of the recombinant enzyme and the native protease are identical. However, the production process of the recombinant Proteinase K guarantees an enzyme of outstanding reliability and purity meeting all requirements of diagnostics' manufacturers.

Special emphasis has been placed on a low DNA-content of the enzyme preparation, making Proteinase K, recombinant, PCR Grade, ideally suited for isolating PCR and RT-PCR templates.

Maximize the yield of target nucleic acids.
Proteinase K is rigorously tested for the absence of nucleases.

Experience stability and flexibility.
The lyophilizate is very stable and can be stored at room temperature for at least 18 months. Lyophilizate dissolved in water is stable for at least 60 days when stored at +2 to +4°C.

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Proteinase K, rec., PCR Grade, lyo.

Proteinase K - a universal tool for nucleic acid template preparation

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Proteinase K, rec., PCR Grade, lyo.

Proteinase K - a universal tool for nucleic acid template preparation

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