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Proteinase K, rec., PCR Grade, lyo.

from Titrirachium album, expressed in Pichia pastoris, lyophilizate

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pcr sample prep

Recombinant Proteinase K in PCR Grade quality is a universal tool for nucleic acid template preparation.

Proteinase K, originally isolated from the mold Tritirachium album, is a recombinant enzyme expressed in Pichia pastoris. It is a highly active, subtilisin-related serine endopeptidases that does not exhibit any pronounced cleavage specificity. Thus, Proteinase K, recombinant, PCR Grade, is a universal tool for template preparation. Amino acid sequence and molecular structure of the recombinant enzyme and the native protease are identical. However, the production process of the recombinant Proteinase K guarantees an enzyme of outstanding reliability and purity meeting all requirements of diagnostics' manufacturers.

Special emphasis has been placed on a low DNA-content of the enzyme preparation, making Proteinase K, recombinant, PCR Grade, ideally suited for isolating PCR and RT-PCR templates.
 

  • Maximize the yield of target nucleic acids.
    Proteinase K is rigorously tested for the absence of nucleases.
  • Experience stability and flexibility.
    The lyophilizate is very stable and can be stored at room temperature for at least 12 months. A broad range of fill volumes from 25 mg to 5 g per vial is available. Lyophilizate dissolved in water is stable for at least 60 days when stored at +2 to +4°C.

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Blocking Reagent
Blocking Reagent powder Use Blocking Reagent to decrease the background in nonradioactive filter hybridization and the detection of nucleic acid hybrids.
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Alkaline Phosphatase, recombinant, 20 U/μL from bovine intestine, expressed in Pichia pastoris, solution Use Alkalkine Phosphatase, recombinant for: Dephosphorylation of 5' phosphate from DNA and RNACoupling to other proteins via its amino or carbohydrate groups, for detection in immunoassays and western blot analysis
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DNase I, rec., GMP Grade, AOF from bovine pancreas, recombinant, expressed in Pichia pastoris, solution DNase I is an essential enzyme for efficient digestion of DNA during RNA purification. For any sensitive RNA-based application, the quality of input RNA matters. Discover how this fit-for-purpose reagent meets your needs.
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KAPA2G Robust HotStart ReadyMix 2x concentrated 2x concentrated, ready-to-use antibody-mediated hot start PCR mix, containing KAPA2G DNA Polymerase in an optimized concentration for amplification of crude sample types and/or AT- or GC-rich targets.
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KAPA3G PCR Buffer 10x concentrated 10x PCR reaction buffer
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NxtScript 2G RT, conc. thermostable reverse transcriptase, 500 U/μL, glycerol-free solution NxtScript 2G RT, conc. is a stand-alone reverse transcriptase. The highly concentrated and glycerol-free formulation makes the product suitable for lyophilization and for the preparation of dry amplification mixes.
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Tth DNA Polymerase from Thermus species, expressed in E. coli, solution Use Tth DNA Polymerase for: One-step RT-PCR of single copy genes from eucaryotic genomes in the presence of Mn2+ ionsOne-step RT-PCR of a specific transcript or an entire population of transcriptsPCR in the presence of Mg2+ ionsLabeling of PCR products with modified nucleotides
Biotin-16-UTP
Biotin-16-UTP 10 mM solution Biotin-16-UTP is a substrate for SP6, T3, and T7 RNA Polymerase. It can replace UTP in the in vitro transcription reaction for RNA labeling.Linearized template DNA with T7, SP6 or T3 promoter is in vitro transcribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP and Biotin-16-UTP, respectively.The labeled RNA can be subsequently detected with a fluorescent streptavidin conjugate or by ELISA using a Streptavidin-AP conjugate.
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FastStart Taq DNA Polymerase, 5 U/μl from Thermus aquaticus BM, expressed in E. coli, solution Use FastStart Taq DNA Polymerase, 5 U/μl, for:Hot start PCR and RT-PCR with high specificity, sensitivity and yieldSpecific amplification of DNA fragments from various sources of DNA and for diverse down-stream applicationsLabeling of DNA with modified nucleotides (e.g., DIG-dUTP, biotin-dUTP, fluorescein-dUTP)The prevention of carryover contamination between PCR reactions in combination with dUTP and Uracil-DNA GlycosylaseManufacture of amplification mixtures for regulated applications (e.g., in vitro diagnostics, quality control) with requests for more stringent validation
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FastStart Taq DNA Polymerase, GMP Grade, 5 U/μl from Thermus aquaticus BM, expressed in E. coli, solution Use FastStart Taq DNA Polymerase, GMP Grade, 5 U/μl, for:Hot start PCR and RT-PCR with high specificity, sensitivity and yieldSpecific amplification of DNA fragments from various sources of DNA and for diverse down-stream applicationsLabeling of DNA with modified nucleotides (e.g., DIG-dUTP, biotin-dUTP, fluorescein-dUTP)The prevention of carryover contamination between PCR reactions in combination with dUTP and Uracil-DNA GlycosylaseManufacture of amplification mixtures for regulated applications (e.g., in vitro diagnostics, quality control) with requests for more stringent validation
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FastStart PCR Buffer 10x conc., with 20 mM MgCl2 Use this buffer together with FastStart Taq DNA Polymerase. For applications refer to FastStart Taq DNA Polymerase, 5 kU, GMP Grade.
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FastStart PCR Buffer 10x conc., without MgCl2 Use this buffer together with FastStart Taq DNA Polymerase whenever the amplification of difficult target requires a specific MgCl2 concentration.
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AptaTaq exo DNA Polymerase, 50 U/μl from Thermus aquaticus BM, expressed in E. coli, glycerol-free solution AptaTaq DNA exo Polymerase is a blend of N-terminal truncated Taq DNA Polymerase and a specific oligonucleotide (aptamer) with hot start features, optimized for excellent discrimination against misextension. This concentrated formulation contains no glycerol and is suitable for the preparation of dried-down amplification mix preparations.
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α-S-dCTP, Molecular Diagnostic Grade S-isomer, sodium salt, 100 mM α-S-dCTP, Molecular Diagnostic Grade, is supplied in sealed and CO2-proof bottles to ensure a stable pH during shipment on dry ice.
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ActiTaq exo Genotyping Master 5x concentrated The 5x concentrated master mix contains a chemically modified truncated Taq DNA Polymerase and all reagents (except primers, probes, and template) needed for real-time DNA detection assays with various probe formats except for hydrolysis probes due to the lack of 5'-3' exonuclease activity.
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AptaTaq DNA Polymerase, 5 U/μl from Thermus aquaticus BM, expressed in E. coli, solution AptaTaq DNA Polymerase is a blend of Taq DNA Polymerase and a specific oligonucleotide (aptamer) providing hot start features.
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AptaTaq Fast PCR Buffer 5x concentrated The composition of this 5x reaction buffer is optimized for fast activation and short PCR reaction times of the AptaTaq DNA Polymerase.
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AptaTaq DNA Polymerase LDx, 50 U/μl from Thermus aquaticus BM, expressed in E. coli, glycerol-free solution AptaTaq DNA Polymerase LDx is a blend of Taq DNA Polymerase and a specific oligonucleotide (aptamer) with hot start features, optimized for applications detecting the lowest levels of DNA.
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NxtScript Reverse Transcriptase, concentrate mutant from Moloney Murine Leukemia Virus, expressed in E. coli Use NxtScript RT for synthesis of cDNA from total RNA or mRNA for:One-step and two-step RT-PCR applicationsRT-PCR for detection of viral targetsRT-PCR for detection of mRNA targets, such as cancer biomarkersTMA and NASBA nucleic acid amplification methodsGeneration of full-length cDNA librariesRapid amplification of cDNA ends (RACE)
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NxtScript Reverse Transcriptase, 85 U/μl mutant from Moloney Murine Leukemia Virus, expressed in E. coli Use NxtScript RT for synthesis of cDNA from total RNA or mRNA for:One-step and two-step RT-PCR applicationsRT-PCR for detection of viral targetsRT-PCR for detection of mRNA targets, such as cancer biomarkersTMA and NASBA nucleic acid amplification methodsGeneration of full-length cDNA librariesRapid amplification of cDNA ends (RACE)
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Water, PCR Grade Use Water, PCR Grade, for the preparation for solutions used in molecular diagnostics or molecular biology.
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NucleoMix (dUTP), PCR Grade sodium salt, 40 mM (10 mmol/L each dNTP) NucleoMix (dUTP), PGR Grade, is supplied in sealed and CO2-proof bottles to ensure a stable pH during shipment on dry ice. The manufacturing process is fully validated including the final filling step. The pH is adjusted to match conditions for amplification.
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Tumor Necrosis Factor-α, human (hTNF-α) recombinant (yeast) Recombinant, Tumor Necrosis Factor-α, human (hTNF-α), is produced in Pichia pastoris and purfied by standard chromatographic techniques.
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Terminal Transferase, recombinant from calf thymus, expressed in E. coli, solution Terminal Transferase catalyzes the template independent addition of deoxy- and dideoxynucleoside triphosphates to the 3'-OH ends of double- and single-stranded DNA fragments and oligonucleotides. Terminal Transferase incorporates digoxigenin-, biotin-, and fluorochrome-labeled deoxy- and dideoxynucleoside triphosphates, as well as radioactively labeled deoxy- and dideoxynucleoside triphosphates. The supplied 5x concentrated reaction buffer facilitates optimal tailing of all types of double-stranded DNA ends: blunt ended, with 3' overhang, or with 5' overhang.
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Transcriptor Reverse Transcriptase recombinant, expressed in E. coli Use Transcriptor Reverse Transcriptase for synthesis of cDNA from total RNA or mRNA for: Two-step RT-PCR applications using conventional thermal cyclers or real-time PCR instrumentsRT-PCR for detection of viral RNATMA and NASBA nucleic acid amplification methodsSynthesis of full-length cDNA up to 14 kb for libraries or cloningRapid amplification of cDNA end (RACE)
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Taq DNA Polymerase, 50 U/μl from Thermus aquaticus BM, expressed in E. coli, glycerol-free solution High concentrated, glycerol-free solution, ideal for preparation of dried-down amplification mixtures.
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Tetramethylrhodamine-5-dUTP 1 mM solution, lithium salt Tetramethylrhodamine-5-dUTP is a substitute for dTTP in nick-translation reactions and in the random-primed labeling technique for DNA labeling, as well as in PCR. The nucleotide also serves as a substrate for Terminal Transferase in 3'-end labeling. Tetramethylrhodamine-labeled probes show red fluorescence and are suitable for use in in situ hybridization for direct fluorescence detection. Multiple fluorescence labeling using Fluorescein-12-dUTP (yellow fluorescence) or other dye-labeled deoxynucleotides is possible.
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T4 DNA Ligase recombinant form of the enzyme from T4 phage, solution (5 x 103 U/ml)
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7-Deaza-2'-dGTP lithium salt, 10 mM Use 7-Deaza-dGTP as a substrate for most DNA polymerases, including Taq DNA polymerase. 7-Deaza-dGTP is used in the dideoxy-chain termination sequencing methods, in place of dGTP to overcome compression problems in gel electrophoresis when sequencing GC-rich stretches of DNA.
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COT Human DNA from human placenta DNA, enriched for repetitive sequences, solution The COT fraction of human genomic DNA consists largely of rapidly annealing repetitive elements. These interspersed repetitive sequences (IRS) such as SINEs (small interspersed repetitive elements, e.g., Alu-elements) and LINEs (large interspersed repetitive elements, e.g., L1-elements) are distributed ubiquitously throughout the genome. COT Human DNA is prepared from human placental DNA by shearing, denaturing, and reannealing under conditions that enrich these repetitive elements.
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COT Human DNA, CGH Grade from human male placenta DNA, enriched for repetitive sequences, solution The COT fraction of human genomic DNA consists largely of rapidly annealing repetitive elements. These interspersed repetitive sequences (IRS) such as SINEs (small interspersed repetitive elements, e.g., Alu-elements) and LINEs (large interspersed repetitive elements, e.g., L1-elements) are distributed ubiquitously throughout the genome. COT Human DNA is prepared from human placental DNA by shearing, denaturing, and reannealing under conditions that enrich these repetitive elements.
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KAPA2G Fast HotStart PCR Kit mutant from Thermus aquaticus, expressed in E. coli, plus 5x reaction buffer The kit contains 2 vials of antibody-mediated hot start DNA polymerase and all buffers necessary to optimize the amplification reaction.
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KAPA Probe Force 2x concentrated 2x concentrated, ready-to-use antibody-mediated hot start PCR mix, containing KAPA3G DNA Polymerase in an optimized concentration for amplification of crude sample types. The master mix contains dUTP for prevention of DNA contamination by PCR carryover by pretreatment with Uracil-DNA Glycosylase.
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KAPA2G Robust HotStart PCR Kit mutant from Thermus aquaticus, expressed in E. coli, plus 5x reaction buffer The kit contains 2 vials of an antibody-mediated hot start DNA polymerase and all buffers necessary to optimize the amplification reaction.
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Poly(A) potassium salt, solution Use Poly(A) as a carrier for quantitative precipitation of DNA and RNA.
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AptaTaq DNA Polymerase, 50 U/μl from Thermus aquaticus BM, expressed in E. coli, glycerol-free solution AptaTaq DNA Polymerase is a blend of Taq DNA Polymerase and a specific oligonucleotide (aptamer) providing hot start features. The concentrated formulation does not contain glycerol and is suitable for the preparation of dry amplification mix preparations.
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M-MLV Reverse Transcriptase, GMP Grade from Moloney Murine Leukemia Virus, expressed in E. coli Use M-MLV Reverse Transcriptase for synthesis of cDNA from total RNA or mRNA for: Two-step RT-PCR applications for amplification from RNA targetsRT-PCR for detection of viral RNATMA and NASBA nucleic acid amplification methodsSynthesis of full-length cDNA for libraries or cloningRapid amplification of cDNA end (RACE)Manufacture of amplification mixtures for applications with regulatory requirements (e.g., in vitro diagnostics, quality control)
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MgCl2 Solution 1.0 M Solution 1.0 M Magnesium chloride solution is filtered using 0.2 μm membrane filters, and filled in sterilized bottles.
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Bovine Serum Albumin, Molecular Biology Grade 2% solution Use Bovine Serum Albumin, Molecular Biology Grade, for enzyme stabilization and for dilution of nucleic acid modifying enzymes.
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Biotin-16-ddUTP 1 mM solution Biotin-16-ddUTP is used as a substrate for:Terminal TransferaseDNA polymerase I (holoenzyme and Klenow fragment)T4 DNA polymeraseTaq DNA polymerase and reverse transcriptase (e.g.,Transcriptor)Biotin-16-ddUTP is preferentially used for 3'-end labeling of oligonucleotides with Terminal Transferase, recombinant.The biotin-labeled oligonucleotide can be used as a hybridization probe for:DNA and RNA transfersColony and plaque screeningIn situ hybridizationIn addition to common hybridization techniques, biotin-labeled oligonucleotides are specifically useful for screening expression libraries for sequence-specific DNA-binding proteins, for example, transcription factors.The labeled oligomer can be subsequently detected by ELISA using the Streptavidin-AP conjugate for nucleic acid detection.Oligonucleotides are enzymatically labeled at their 3' end with Terminal Transferase by incorporation of a single biotin-labeled dideoxyuridine-triphosphate (biotin-ddUTP).
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dITP, PCR Grade sodium salt, 100 mM dITP, PGR Grade, is supplied in sealed and CO2-proof bottles to ensure a stable pH during shipment on dry ice. The manufacturing process is fully validated including the final filling step. The pH is adjusted to match conditions for amplification.
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Uracil-DNA Glycosylase, heat-labile from marine bacterium BMTU 3346, expressed in E. coli Use Uracil-DNA Glycosylase, heat-labile, (UNG) for prevention of carryover contamination with DNA amplification products in PCR. Always use dUTP containing PCR mixtures to enable decontamination by UNG treatment. In contrast to the UNG variant from E.coli, this heat-labile enzyme is completely inactivated in the initial heat denaturation step of a common PCR protocol and the formed PCR product will not be degraded.Note: For high sensitive real-time PCR, specially optimized LightCycler® Uracil-DNA Glycosylase is recommended.
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Expand High Fidelity PCR System Polymerase and buffer system for the generation of DNA fragments ranging from 3 kb to 27 kb Enzyme blend consisting of Taq DNA Polymerase and Tgo DNA Polymerase.
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RNase A from bovine pancreas, lyophilizate, powder Use RNase A for isolation of genomic DNA. For this purpose, RNase A should be boiled before use.
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ATP, Molecular Diagnostic Grade sodium salt, 325 mM ATP, Molecular Diagnostic Grade, is supplied in sealed and CO2-proof bottles to ensure a stable pH during shipment on dry ice. The manufacturing process is fully validated including the final filling step. The pH is adjusted to match conditions for reverse transcription and amplification.
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Fluorescein-12-dUTP 1 mM solution Use Fluorescein-12-dUTP as a substrate for:Terminal TransferaseDNA polymerase I (holoenzyme and Klenow fragment)Taq DNA polymeraseReverse transcriptase (e.g., from AMV and M-MuLV)Fluorescein-12-dUTP replaces dTTP in the random-primed DNA labeling reaction or in nick translation reactions, as well as in PCR. The nucleotide also serves as a substrate for Terminal Transferase in 3'-end labeling.Fluorescein-labeled probes can be used for in situ hybridization with direct fluorescence detection and detection by ELISA using Anti-Fluorescein-AP, Fab fragments.Repeated fluorescence labeling using Tetramethylrhodamine-6-dUTP (red) and AMCA-6-dUTP (bright blue) is possible.
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FastStart Taq DNA Polymerase, 100 U/μl from Thermus aquaticus BM, expressed in E. coli, solution Use FastStart Taq DNA Polymerase, 100 U/μl, especially for:Setup of PCR master mixtures, when highly concentrated components are requestedPreparation of stabilized dried-down formulations of reaction mixturesFor further applications, see FastStart Taq DNA Polymerase, GMP Grade, 5 U/μl
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NucleoMix (dTTP), PCR Grade sodium salt, 40 mM (10 mmol/L each dNTP) NucleoMix (dTTP), PGR Grade, is supplied in sealed and CO2-proof bottles to ensure a stable pH during shipment on dry ice. The manufacturing process is fully validated including the final filling step. The pH is adjusted to match conditions for amplification.
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GTP, Molecular Diagnostic Grade sodium salt, 325 mM GTP, Molecular Diagnostic Grade, is supplied in sealed and CO2-proof bottles to ensure a stable pH during shipment on dry ice. The manufacturing process is fully validated including the final filling step. The pH is adjusted to match conditions for reverse transcription and amplification.
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Taq DNA Polymerase, 5 U/μl from Thermus aquaticus BM, expressed in E. coli, solution Use Taq DNA Polymerase, GMP Grade, 5 U/μl, for:Routine PCR and RT-PCR applicationsAmplification of DNA fragments up to 3 kb from various sources of DNALabeling of DNA with modified nucleotides (e.g., DIG-dUTP, biotin-dUTP, fluorescein-dUTP)Combination with dUTP and Uracil-DNA Glycosylase for prevention of carryover contamination between PCR reactionsManufacture of amplification mixtures for applications with regulatory requirements (e.g.,in vitro diagnostics, quality control)
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dTTP, PCR Grade sodium salt, 100 mM dTTP, PGR Grade, is supplied in sealed and CO2-proof bottles to ensure a stable pH during shipment on dry ice. The manufacturing process is fully validated including the final filling step. The pH is adjusted to match conditions for amplification.
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dATP, PCR Grade sodium salt, 100 mM dATP, PGR Grade, is supplied in sealed and CO2-proof bottles to ensure a stable pH during shipment on dry ice. The manufacturing process is fully validated including the final filling step. The pH is adjusted to match conditions for amplification.
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dUTP, PCR Grade sodium salt, 100 mM dUTP, PGR Grade, is supplied in sealed and CO2-proof bottles to ensure a stable pH during shipment on dry ice. The manufacturing process is fully validated including the final filling step. The pH is adjusted to match conditions for amplification.
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dCTP, PCR Grade sodium salt, 100 mM dCTP, PGR Grade, is supplied in sealed and CO2-proof bottles to ensure a stable pH during shipment on dry ice. The manufacturing process is fully validated including the final filling step. The pH is adjusted to match conditions for amplification.
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UTP, Molecular Diagnostic Grade sodium salt, 325 mM UTP, Molecular Diagnostic Grade, is supplied in sealed and CO2-proof bottles to ensure a stable pH during shipment on dry ice. The manufacturing process is fully validated including the final filling step. The pH is adjusted to match conditions for reverse transcription and amplification.
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CTP, Molecular Diagnostic Grade sodium salt, 325 mM CTP, Molecular Diagnostic Grade, is supplied in sealed and CO2-proof bottles to ensure a stable pH during shipment on dry ice. The manufacturing process is fully validated including the final filling step. The pH is adjusted to match conditions for reverse transcription and amplification.
NucleoMix, 400 mM (100 mM each)
NucleoMix, 400 mM (100 mM each) sodium salt solution Ready-to-use mixture of dATP, dCTP, dGTP and dTTP.
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AptaTaq Genotyping Master 5x concentrated AptaTaq DNA Master is a 5x concentrated, ready-to-use, one component hot start PCR mix, containing AptaTaq DNA Polymerase in an optimized concentration for the amplification of difficult sample types, reaction buffer, and a dNTP mix using dUTP instead of dTTP (for prevention of DNA contamination by PCR carryover by pretreatment with Uracil-DNA Glycosylase).
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NxtScript DNA Master 5x concentrated NxtScript DNA Master is a 5x concentrated, ready-to-use, one component hot start PCR mix, containing AptaTaq DNA Polymerase in an optimized concentration for multiplex qPCR or qRT-PCR. It uses aptamer-mediated reversible hot start technology for specific priming and fast PCR. The mix contains dNTP mix with dUTP for prevention of DNA contamination by PCR carryover by pretreatment with Uracil-DNA Glycosylase.
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EvoScript RNA Master 5x concentrated EvoScript RNA Master is a perfect master mix for convenient RT-qPCR reactions. It includes FastStart Taq DNA Polymerase and a designer polymerase for reverse transcription. It uses a sophisticated hot start system that includes chemical modification and aptamer-mediated hot start. This enables highly specific priming for both, reverse transcription and DNA amplification. The 1-vial composition is ideally suited for easy reaction assembly with just the addition of oligos and target RNA.
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dGTP, PCR Grade sodium salt, 100 mM dGTP, PGR Grade, is supplied in sealed and CO2-proof bottles to ensure a stable pH during shipment on dry ice. The manufacturing process is fully validated including the final filling step. The pH is adjusted to match conditions for amplification.
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Guanidine Hydrochloride crystals Use Guanidine hydrochloride as a denaturing agent for proteins in a broad variety of nucleic acid purification applications.
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DNA from fish sperm, lyophilizate DNA is resuspended in sterile double-distilled water at the desired concentration and sheared to an average fragment length of 100 to 3,000 bp.
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HawkZ05 Fast One-Step RT-PCR Master 5x concentrated, 0.5% glycerol content HawkZ05 Fast One-Step RT-PCR Kit is supplied as a kit containing 2 vials of HawkZ05 Fast One-Step RT-PCR Master Mix and 2 vials of RMS Manganese Acetate (25 mM). The formulation contains 0.5% glycerol and is especially suited for the preparation of dry amplification mix preparations.
LSR For life science research only. Not for use in diagnostic procedures.
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HawkZ05 Fast One-Step RT-PCR Master (Rox) 2.3x concentrated HawkZ05 Fast One-Step RT-PCR Kit is supplied as a kit containing 1 vial of HawkZ05 Fast One-Step RT-PCR Master Mix and 1 vial of RMS Manganese Acetate (25 mM). The master mix contains a reference dye (FRET-ROX) to run assays on real-time PCR instruments which require Rox for quantitative analysis.
DNase I
DNase I, rec., RNase-free, GMP Grade from bovine pancreas, expressed in Pichia pastoris, lyophilizate DNase I is a DNA-specific endonuclease that hydrolyzes the phosphodiester linkages of double- and single-stranded DNA to a mixture of mono- and oligonucleotides. It digests DNA templates thereby purifing (m)RNA during in vitro synthesis. In molecular diagnostics applications, DNase I can be used to prepare genomic DNA-free RNA templates for RT-PCR.
RNase Inhibitor, rec., lyo-ready
RNase Inhibitor, rec., lyo-ready from rat lung, expressed in E. coli, glycerol-free solution Manufactured in an ISO-13485 certified facility, RNase Inhibitor, lyo-ready, offers outstanding performance and peace of mind for manufacturers of molecular IVD assays.
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NucleoMix (dTTP), PCR Grade sodium salt, 100 mM (25 mmol/L each dNTP) NucleoMix (dTTP), PGR Grade, is supplied in sealed and CO2-proof bottles to ensure a stable pH during shipment on dry ice. The manufacturing process is fully validated including the final filling step. The pH is adjusted to match conditions for amplification.
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Taq DNA Polymerase, GMP Grade, 5 U/μl from Thermus aquaticus BM, expressed in E. coli, solution Use Taq DNA Polymerase, GMP Grade, 5 U/μl, for:Routine PCR and RT-PCR applicationsAmplification of DNA fragments up to 3 kb from various sources of DNALabeling of DNA with modified nucleotides (e.g., DIG-dUTP, biotin-dUTP, fluorescein-dUTP)Combination with dUTP and Uracil-DNA Glycosylase for prevention of carryover contamination between PCR reactionsManufacture of amplification mixtures for applications with regulatory requirements (e.g.,in vitro diagnostics, quality control)
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DNase I, rec., Grade I from bovine pancreas, expressed in Pichia pastoris, lyophilizate DNase I, recombinant, Grade I, originally isolated from bovine pancreas, is a recombinant enzyme expressed in Pichia pastoris. It is a glycoprotein of a molecular weight of approximately 39 kD. DNase I, recombinant, Grade I, is a DNA-specific endonuclease that hydrolyzes phosphodiester linkages of double- and single-stranded DNA to a mixture of mono- and oligonucleotides.DNase I, recombinant, Grade I, is manufactured using state-of-the-art processes yielding animal component-free material.
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Expand High Fidelity PCR Buffer 10x conc., with MgCl2 Use this buffer together with Expand High Fidelity PCR System.
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AptaTaq exo DNA Polymerase, 5 U/μl from Thermus aquaticus BM, expressed in E. coli, solution This novel optimized mixture of high-quality N-terminal-deleted Taq DNA Polymerase and a specific oligonucleotide (aptamer) provides improved discrimination against misextension. As with the AptaTaq DNA Polymerase System, the AptaTaq exo DNA Polymerase-based assay shows high specificity and a broad dynamic range of products.
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KAPA3G HotStart DNA Polymerase, Glycerol-free, 30 U/µL from Thermus aquaticus, expressed in E. coli The product consists of antibody-mediated KAPA3G HotStart DNA polymerase at 30 U/µL.
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HawkZ05 Fast DNA Polymerase, 200 U/μl mutant from Thermus species Z05, recombinant in E. coli, glycerol-free solution HawkZ05 Fast DNA Polymerase is a blend of Z05 DNA Polymerase and a specific oligonucleotide (aptamer) providing hot start feature. The concentrated, glycerol-free formulation is ready for lyophilization and suitable for the preparation of dry amplification mix preparations.
NxtScript Reverse Transcriptase, high conc., 1MU
NxtScript Reverse Transcriptase, high conc., 1MU mutant from Moloney Murine Leukemia Virus, expressed in E.coli NxtScript RT high. Conc. Is a stand-alone reverse transcriptase. The highly concentrated formulation makes the product more suitable for lyophilization and for the preparation of dry amplification mix preparations.
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