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For further processing into IVD products and medical devices only. Others Proteinase K rec lyo-ready Proteinase K, rec., lyo-ready PID00001275 from Titrirachium album, expressed in Pichia pastoris, solution 10 575 506 103 10575506103 Proteinase K rec., Lyo ready Proteinase K rec., Lyo ready Reagents, kits custom fill Not Available CustomBiotech product. Please contact your local representative. Will be supplied as "Proteinase K, rec., lyo-ready". Unit of measure is "mL". 39450-01-6 en 3.4.21.64 en Proteinase K is essential to eliminate protein contamination from DNA and RNA preparations. The glycerol-free formulation with a high concentration of 40mg/mL is compatible with dried-down applications and therefore makes it a valuable tool for molecular assay developers and manufacturers. en Glycerol-free formulation suitable for lyophilization and drying down (lyo-ready). en Proteinase K, rec., lyo-ready digests proteins very efficiently. It is essential to rapidly inactivate endogenous RNases and DNases during nucleic acid isolation. en Appearance: Clear, colorless solution
Volume activity (+37°C, hemoglobin): ≥ 1460 U/mL
Specific activity (+37°C, hemoglobin): ≥ 30 U/mg protein
Unit definition (hemoglobin): One unit is the enzyme activity which releases folin positive amino acids and peptides equivalent to 1 μmol of tyrosine in 1 minute under the test conditions at 37°C.
Volume activity (+25°C, Chromozym): ≥ 122 U/mL
Specific activity (+25°C, Chromozym): ≥ 2.5 U/mg protein
Unit definition (Chromozym): One unit is the enzyme activity which cleaves 18 mmol Chromozym TRY in 1 minute at +25°C.
Protein (Biuret): 40-48 mg/mL
Unspecific endonucleases (MWM III DNA): Not detectable in up to 200 μg after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 200 μg after 16 hours incubation at +37°C.
Ribonucleases (MS2 RNA): Not detectable in up to 40 μg after 16 hours incubation at +37°C. Host Cell DNA : ≤ 1 pg/mg enzyme
Human Genomic DNA: ≤ 4 ng/mg enzyme Bioburden: ≤ 5 CFU/mL
Stability: At -15 to -25°C within specification range for 18 months. en