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"Health_Topics", "NameofStructureSystem": "Health Topics", "StructureNodeID": "99-00-00", "StructureGroupPath": "NOT APPLICABLE", "StructureGroupName": "NOT APPLICABLE" } ] } ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Language": "en", "Value": "Keep at +2 to +15°C for on-board use.
After first use on the instrument, the reagents are stable for up to 12 weeks.
", "Name": "Storage Conditions (Working Solution)" }, { "Language": "en", "Value": "Store the reagent cassette at +2 to +8°C.", "Name": "Storage Conditions (Product)" }, { "Language": "en", "Value": "
Phosphate concentration can be determined in following range:
  • PHO2B: 9.5 - 795 mg/L, (0.1 - 8.37 mmol/L)
  • PHO2D: 105 - 8.745 mg/L (1.1 - 92 mmol/L),
up to 96 g/L (1 mol/L) with postdilution

Conversion Factors
Conversion factors for phosphate concentration:
  • 1 mmol/L = 94.97 mg/L
  • 1 mg/L = 10.53 μmol/L

Traceability
This method has been standardized against a primary reference material (NERL).

Precision
Representative performance data on Cedex Bio HT Analyzers are shown. Results obtained in individual laboratories may differ.
Precision was determined in samples of two concentration levels. Coefficients of variation (CV) were calculated for in-run precision (n=21) and inter-run precision (on 21 days).

 Level 1Level 2
Mean13.9 mmol/L (431 mg/L)27.7 mmol/L (859 mg/L)
CV in-run1.0%0.7%
CV inter-run1.7%1.1%
", "Name": "Background Information - Help Corner" }, { "Language": "en", "Value": "
Analyzer and accessories
  • Cedex Bio HT Analyzer*, including accessories and disposables
  • Standard laboratory equipment
Calibrators and Controls
  • Calibrator A Bio*
  • Control A Level 1 Bio*
  • Control A Level 2 Bio*
  • Control A Level 3 Bio*
", "Name": "Additional Equipment and Reagent Required" }, { "Language": "en", "Value": "Kit for determination of phosphate.", "Name": "Product Description" }, { "Language": "en", "Value": "Unit of measure is \"piece\".", "Name": "CB - Order Information" }, { "Language": "en", "Value": "Application PHO2B (742), PHO2D (773)
Cedex Bio HT test definition:
  • Measuring mode\t\t\t\t\tAbsorbance
  • Abs. calculation mode\t\t   Endpoint
  • Reaction direction\t\t\t\t  Increase
  • Wavelength A/B\t\t\t\t\t   340/659 nm
  • Calc. first/last\t\t\t\t\t\t   33/63
  • Unit\t\t\t\t\t\t\t\t\t\t\tmmol/L
  • Reaction mode\t\t\t\t\t\tD-R1-S-SR
  • Predilution factor\t\t\t\t\t11

Pipetting parameters:
  • R\t\t\t\t\t\t\t\t\t\t\t\t190 μL
  • Sample\t\t\t\t\t\t\t\t\t 2.5 μL + 27.5 μL Diluent (H2O)
  • SR\t\t\t\t\t\t\t\t\t\t\t  38 μL
  • Total volume\t\t\t\t\t\t\t158 μL

Calibration:
  • Calibrator\t\t\t\t\t\t\t\t Calibrator A Bio (CAL A, 599)
  • Calibration mode\t\t\t\t   Linear regression
  • Calibration interval\t\t\t\t Each new lot, and if recalibration is required due to QC results

Quality control:
  • Control A Level 1 Bio (CONA1, 111)
  • Control A Level 2 Bio (CONA2, 112)
  • Control A Level 3 Bio (CONA3, 113)

Use the recommended control material. Other suitable control material can be used in addition.
Control intervals and acceptance limits should be adapted to each laboratory's individual requirements.
If values do not fall within the defined limits, corrective measures and recalibration are required", "Name": "Protocols - Help Corner" }, { "Language": "en", "Value": "
VialContentComposition
PHO2BReagent cassette,
250 tests
Reagent 1 (R1): Sulfuric acid, 0.36 mol/L; non-reactive surfactant.
Start Reagent (SR): Ammonium molybdate, 3.5 mmol/L; sulfuric acid, 0.36 mol/L; sodium chloride, 150 mmol/L.
", "Name": "Content" }, { "Language": "en", "Value": "Phosphate is a mineral nutrient, which is reqired in cell cultures and microbial fermentation for optimal growth and product formation. For control of culture media and for monitoring in a culture process, the phosphate concentration can be determined on a Cedex Bio or Cedex Bio HT Analyzer with high reliability and convenience.", "Name": "Applications" }, { "Language": "en", "Value": "Method: Colorimetric detection with ammonium molybdate.
Recovery: ±10%", "Name": "Specification" }, { "Language": "en", "Value": "Precautions
  • Handle all samples as if potentially infectious, using safe laboratory procedures. As the sensitivity and titer of potential pathogens in the sample material can vary, the operator must optimize pathogen inactivation and follow the appropriate measures according to local safety regulations.
  • Do not eat, drink, or smoke in the laboratory work area.
  • Do not pipette by mouth.
  • Wear protective disposable gloves, laboratory coats, and eye pro\u00adtection, when handling samples and kit reagents.
  • Wash hands thoroughly after handling samples and reagents.
Waste handling
  • Discard unused reagents and waste in accordance with country, federal, state, and local regulations.
  • Safety Data Sheets (SDS) are available online at dialog.roche.com, or upon request from the local Roche office.
", "Name": "Safety Information" } ] } } ] }

Phosphate Bio HT

Test kit for the Cedex Bio HT Analyzer

Phosphate Bio HT

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