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After first use on the instrument, the reagents are stable for up to 16 weeks.", "Name": "Storage Conditions (Working Solution)" }, { "Language": "en", "Value": "Store the reagent cassette at +15 to +25°C.", "Name": "Storage Conditions (Product)" }, { "Language": "en", "Value": "This product is intended for determination of optical density using the Cedex Bio HT Analyzer for biomass measurements during microbial fermentation.", "Name": "Product Description" }, { "Language": "en", "Value": "
VialContentComposition
ODBReagent cassette,
400 tests
Reagent: Phosphate buffered saline, stabilizer; pH 7.0.
", "Name": "Content" }, { "Language": "en", "Value": "Measuring Range
The optical density of microbial suspensions can be determined in following ranges:
  • ODB: 0.1 to 10 OD
  • ODD: 3 to 360 OD

Precision
Representative performance data on Cedex Bio HT Analyzers are shown. Results obtained in individual laboratories may differ.
Precision was determined in samples of three concentration levels. Coefficients of variation (CV) were calculated for in-run precision (n=21) and inter-run precision (on 10 days).

                            Level 1              Level 2                 Level 3
Mean OD 2 20 150
CV in-run 1.5% 1.7% 1.6%
CV inter-run 1.9% 1.7% 1.7%
", "Name": "Background Information - Help Corner" }, { "Language": "en", "Value": "Unit of measure is \"piece\".", "Name": "CB - Order Information" }, { "Language": "en", "Value": "Application ODB (257), ODD (277)
ODB is the sensitive protocol for determination of low optical density up to OD 10 in the starting phase of a fermentation.
If the optical density is expected to be higher than OD 10, then protocol ODD should be used (same protocol with preset 1:30 predilution step).
Samples must be resuspended thoroughly before testing (e. g. using a vortex mixer), and the OD test should be started within 3 min. after mixing to avoid the risk of interference by sedimentation. The speed of sedimentation can vary depending on species, medium and cell density.
If additional tests should be performed for a sample, then the OD test should be started first. Other tests should be ordered after sampling for the OD test has been completed.

Cedex Bio HT test definition:
Measuring mode                         Absorbance
Abs. calculation mode                Endpoint
Reaction direction                       Increase
Wavelength A/B                            583 nm
Calc. first/last                               17/19
Unit                                                 OD
Reaction mode                            R1-S

Pipetting parameters:
R1                                                  18 μL + 142 μL Diluent (H2O)
Sample                                         20 μL + 20 μL Diluent (H2O)
Total volume                              200 μL

Calibration:
No test-specific calibration is required for the OD test.
The factory-made calibration of the photometer unit of Cedex Bio HT Instruments is stable and highly equivalent between the instruments.

Quality control:
Control OD Level 1 Bio (CONO1, 07-1158-6)
Control OD Level 2 Bio (CONO2, 07-1159-4)
Control OD Level 3 Bio (CONO3, 07-1160-8)
Use the recommended control material. Other suitable control material can be used in addition.
Intervals / limitsControl intervals and acceptance limits should be adapted to each laboratory's individual
requirements.", "Name": "Protocols - Help Corner" }, { "Language": "en", "Value": "The optical absorbance at 583 nm is measured photometrically using the Cedex Bio HT Analyzer. The absorbance value (OD) is proportional to the amount of biomass in the sample.", "Name": "Principle" }, { "Language": "en", "Value": "
Analyzer and accessories
  • Cedex Bio HT Analyzer*, including accessories and disposables
  • Standard laboratory equipment
Calibrators and Controls
  • Control OD Level 1 Bio*
  • Control OD Level 2 Bio*
  • Control OD Level 3 Bio*
", "Name": "Additional Equipment and Reagent Required" }, { "Language": "en", "Value": "The most common method to monitor the growth in bacterial and yeast cultures is the determination of the optical density of the culture suspension, which relates to the number of cells per culture volume. The manual procedure to prepare the required diutions and to perform the measurements on a photometer is usually very imprecise, error-prone and laborious. The automated testing using a Cedex Bio or Cedex Bio HT Analyzer, offers a standardized OD determination with high precison, reliability and convenience.
", "Name": "Applications" }, { "Language": "en", "Value": "Method: Automated sample dilution and photometric determination at 583 nm.
Recovery: ±15%", "Name": "Specification" } ] } } ] }

OD Bio HT

Test kit for the Cedex Bio HT Analyzer

Optical Density Cedex Bio HT

Overview

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Technical documents

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