{ "ProductData": { "RelatedLinks": "", "RegulatoryDisclaimer1": "", "RegulatoryDisclaimer2": "", "StainLocalization": [ "" ], "DisclaimerGroup2": null, "ProductNameGlobal": "NxtScript Reverse Transcriptase concentrate", "DisclaimerGroup1": null, "ControlTissue": [ "" ], "BrandName": "NxtScript Reverse Transcriptase, concentrate", "LicenseDisclaimers": [ ], "Isotypes": "", "RegulatoryDisclaimer3": "For customers in the European Economic Area: Contains SVHC: octyl/nonylphenol ethoxylates. For further processing on its own or in a mixture as part of an IVD method and under controlled conditions only – acc. to Art. 56 (3) and 3 no. 23 REACH Regulation.", "ID": "3.6.14.3.11.1", "ProductNameAddition": "mutant from Moloney Murine Leukemia Virus, expressed in E. coli", "Species": [ "" ], "Clone": "" }, "ProductSpec": { "ProductSpecVariant": { "Chapters": [ { "Language": "English", "Value": "Will be supplied as \"NxtScript RT, conc.\". Unit of measure is \"MU\".
", "Name": "CB - Order Information" }, { "Language": "English", "Value": "NxtScript reverse transcriptase is highly thermostable and allows higher temperatures for reverse transcription, thus providing excellent results for difficult RNA targets.
Enzyme activities: RNA-dependent DNA polymerase, DNA-dependent DNA polymerase, low RNase H activity, no endonuclease activity.
Recommended reaction temperature: +42 to +55°C
Substrates: Incorporates dNTP, ddNTP, dUTP, and various labeled or modified nucleotides.
Divalent ion requirement: Mg²⁺", "Name": "CB - Properties" }, { "Language": "English", "Value": "Appearance: Clear, colorless solution
Activity: ≥250 U/μL
Unit definition: One unit NxtScript Reverse Transcriptase is defined as the amount of enzyme which incorporates 1nmol [³H]TMP into an acid insoluble product in 10 minutes at +37°C with poly(A)x(dT)₁₅ as substrate.
Purity (HPLC): ≥90%
Unspecific endonucleases (MWM III DNA): Not detectable in up to 75 U after 16 hours incubation at +37°C.
Ribonucleases (MS2 RNA): Not detectable in up to 150 U after 1 hour incubation at +37°C.
Stability: At -15 to -25°C wihtin specification range for 12 months.", "Name": "CB - Specification" }, { "Language": "English", "Value": "Use NxtScript RT for synthesis of cDNA from total RNA or mRNA for:

• Two-step RT-PCR applications
• RT-PCR for detection of viral targets
• RT-PCR for detection of mRNA targets, such as cancer biomarkers
• TMA and NASBA nucleic acid amplification methods
• Generation of full-length cDNA libraries
• Rapid amplification of cDNA ends (RACE)

", "Name": "Applications" }, { "Language": "English", "Value": "M-MLV Reverse Transcriptase mutant designed for high thermostability and comparable performance to the market leader.", "Name": "Positioning" }, { "Language": "English", "Value": "

• Reverse transcribe difficult templates.
  The high thermostability of NxtScript allows reactions up to +60°C to overcome RNA secondary structures (e.g., in GC-rich templates).
• Achieve higher yield.
  NxtScript RT lacks RNase H activity. This results in higher cDNA yields.
• Stay specific.
  Make use of the wide temperature activity range of NxtScript and reverse transcribe at the temperature that is optimal for your RNA target.

", "Name": "Benefits" }, { "Language": "English", "Value": "Appearance: Clear, colorless solution
Activity: ≥250 U/μL
Unit definition: One unit NxtScript Reverse Transcriptase is defined as the amount of enzyme which incorporates 1nmol [³H]TMP into an acid insoluble product in 10 minutes at +37°C with poly(A)x(dT)₁₅ as substrate.
Purity (HPLC): ≥90%
Unspecific endonucleases (MWM III DNA): Not detectable in up to 75 U after 16 hours incubation at +37°C.
Ribonucleases (MS2 RNA): Not detectable in up to 150 U after 1 hour incubation at +37°C.
Stability: At -15 to -25°C wihtin specification range for 12 months.", "Name": "Specification" }, { "Language": "English", "Value": "NxtScript reverse transcriptase is highly thermostable and allows higher temperatures for reverse transcription, thus providing excellent results for difficult RNA targets.
Enzyme activities: RNA-dependent DNA polymerase, DNA-dependent DNA polymerase, low RNase H activity, no endonuclease activity.
Recommended reaction temperature: +42 to +55°C
Substrates: Incorporates dNTP, ddNTP, dUTP, and various labeled or modified nucleotides.
Divalent ion requirement: Mg²⁺", "Name": "Properties" } ] } }, "Product2Taxomony": { "Product2TaxomonyReferences": [ { "StructureSystemName": "OWP Product Solutions", "NodeName": "CustomBiotech", "StructureSystemID": null, "NodeID": "520" }, { "StructureSystemName": "OWP Family", "NodeName": "NxtScript", "StructureSystemID": null, "NodeID": "520" }, { "StructureSystemName": "OWP Product Types", "NodeName": "Raw Materials and Reagents->Reverse Transcriptases", "StructureSystemID": null, "NodeID": "70-047-00" }, { "StructureSystemName": "Product Grouping", "NodeName": "CustomBiotech->Molecular Raw Materials", "StructureSystemID": null, "NodeID": "02-0136" }, { "StructureSystemName": "OWP Organization", "NodeName": "Roche Diagnostics Solution (RDS)->Customer Areas->Core lab->CustomBiotech and LifeScience", "StructureSystemID": null, "NodeID": "01-01-01-07-00" }, { "StructureSystemName": "Product Grouping", "NodeName": "CustomBiotech->Enzymes", "StructureSystemID": null, "NodeID": "02-0014" }, { "StructureSystemName": "OWP Techniques", "NodeName": "RT-PCR/qRT-PCR", "StructureSystemID": null, "NodeID": "300-00" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "07051166103", "MaterialDescription": "NxtScript RT, conc.", "RegisteredProductName": "", "GTIN": "07613336132807", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "custom fill", "PackSizeDescPIM360": "", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"NxtScript RT, conc.\". Unit of measure is \"MU\".
" } ] }, "Product2Products": { "Product2ProductReference": [ { "ProductID": "3.6.14.3.11.4", "BrandName": "NxtScript 2G RT, conc.", "ProductNameAddition": "thermostable reverse transcriptase, 500 U/μL, glycerol-free solution", "ReferenceType": "Reagent" }, { "ProductID": "3.5.9.5.2.11", "BrandName": "NxtScript DNA Master", "ProductNameAddition": "5x concentrated", "ReferenceType": "Reagent" } ] } }