For further processing only. Others NxtScript Reverse Transcriptase concentrate NxtScript Reverse Transcriptase, concentrate 3.6.14.3.11.1 mutant from Moloney Murine Leukemia Virus, expressed in E. coli 07 051 166 103 7 051 166 103 07051166103 7051166103 07051166103 NxtScript RT, conc. NxtScript Reverse Transcriptase, concentrate 07613336132807 Reagents, kits custom fill Not Available CustomBiotech product. Please contact your local representative. Will be supplied as "NxtScript RT, conc.". Unit of measure is "MU". EC 2.7.7.49 en Use NxtScript RT for synthesis of cDNA from total RNA or mRNA for:One-step and two-step RT-PCR applicationsRT-PCR for detection of viral targetsRT-PCR for detection of mRNA targets, such as cancer biomarkersTMA and NASBA nucleic acid amplification methodsGeneration of full-length cDNA librariesRapid amplification of cDNA ends (RACE) en M-MLV Reverse Transcriptase mutant designed for high thermostability and comparable performance to the market leader. en Reverse transcribe difficult templates. The high thermostability of NxtScript allows reactions up to +60°C to overcome RNA secondary structures (e.g., in GC-rich templates). Achieve higher yield. NxtScript RT lacks RNase H activity. This results in higher cDNA yields. Stay specific. Make use of the wide temperature activity range of NxtScript and reverse transcribe at the temperature that is optimal for your RNA target. en Appearance: Clear, colorless solutionActivity: 750 - 2200 U/μLPurity (HPLC): ≥90%Unspecific endonucleases (MWM III DNA): Not detectable in up to 75 U after 16 hours incubation at +37°C.Ribonucleases (MS2 RNA): Not detectable in up to 150 U after 1 hour incubation at +37°C.Stability: At -15 to -25°C wihtin specification range for 12 months. en

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