{ "ProductData": { "RelatedLinks": "", "RegulatoryDisclaimer1": "For further processing only.", "RegulatoryDisclaimer2": "", "StainLocalization": [ "" ], "ProductType": "Others", "DisclaimerGroup2": null, "ProductNameGlobal": "NxtScript Reverse Transcriptase 85 U uL", "DisclaimerGroup1": "Non-IVD", "ControlTissue": [ "" ], "BrandName": "NxtScript Reverse Transcriptase, 85 U/μl", "LicenseDisclaimers": [ ], "Isotypes": "", "RegulatoryDisclaimer3": "", "ID": "3.6.14.3.11.2", "ProductNameAddition": "mutant from Moloney Murine Leukemia Virus, expressed in E. coli", "Species": [ "" ], "Clone": "" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/CB_IMAGE.png", "ImageType": "Image main" }, "Product2Taxomony": { "Product2TaxomonyReferences": [ { "StructureSystemIdentifier": "OWP_Product_Solutions", "StructureSystemName": "OWP Product Solutions", "NodeName": "CustomBiotech", "NodeID": "520" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Family", "NodeName": "NxtScript", "NodeID": "520" }, { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "OWP Product Types", "NodeName": "Raw Materials->Reverse Transcriptases", "NodeID": "70-047-00" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "CustomBiotech->Molecular Raw Materials", "NodeID": "02-0136" }, { "StructureSystemIdentifier": "OWP_Organization", "StructureSystemName": "OWP Organization", "NodeName": "Roche Diagnostics Solution (RDS)->Customer Areas->Core lab->CustomBiotech and LifeScience", "NodeID": "01-01-01-07-00" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "CustomBiotech->Enzymes", "NodeID": "02-0014" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "OWP Techniques", "NodeName": "RT-PCR/qRT-PCR", "NodeID": "300-00" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "NodeName": "CustomBiotech", "NodeID": "520" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "07371527103", "MaterialDescription": "NxtScript RT, 85 U/ul 80 ul", "RegisteredProductName": "NxtScript Reverse Transcriptase, 85 U/?L", "GTIN": "", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "80 μL", "PackSizeDescPIM360": "85 U/μL", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"NxtScript RT, 85 U/μL, 80 μL\". Unit of measure is \"piece\"." } ] }, "Product2Products": { "Product2ProductReference": [ { "ProductID": "3.5.9.5.2.11", "BrandName": "NxtScript DNA Master", "ProductNameAddition": "5x concentrated", "ReferenceType": "Reagent" } ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Language": "en", "Value": "Will be supplied as \"NxtScript RT, 85 U/μL, 80 μL\". Unit of measure is \"piece\".", "Name": "CB - Order Information" }, { "Language": "en", "Value": "M-MLV Reverse Transcriptase mutant designed for high thermostability and comparable performance to the market leader.", "Name": "Positioning" }, { "Language": "en", "Value": "Use NxtScript RT for synthesis of cDNA from total RNA or mRNA for:

• Two-step RT-PCR applications
• RT-PCR for detection of viral targets
• RT-PCR for detection of mRNA targets, such as cancer biomarkers
• TMA and NASBA nucleic acid amplification methods
• Generation of full-length cDNA libraries
• Rapid amplification of cDNA ends (RACE)

", "Name": "Applications" }, { "Language": "en", "Value": "

• Reverse transcribe difficult templates.
  The high thermostability of NxtScript allows reactions up to +60°C to overcome RNA secondary structures (e.g., in GC-rich templates).
• Achieve high sensitivity.
  NxtScript lacks RNase H activity. This results in higher cDNA yields.
• Stay specific.
  Make use of the wide temperature activity range of NxtScript and reverse transcribe at the temperature that is optimal for your RNA target.
• Convenience.
  Apply this enzyme directly to your reaction mix without the need for dilution

", "Name": "Benefits" }, { "Language": "en", "Value": "Appearance: White cap, clear colorless solution
Function test in qPCR (human reference cDNA G6PDH/β2M fragments): Corresponds to reference
Stability: At -15 to -25°C wihtin specification range for 40 months.", "Name": "Specification" }, { "Language": "en", "Value": "NxtScript reverse transcriptase is highly thermostable and allows higher temperatures for reverse transcription, thus providing excellent results for difficult RNA targets.
Enzyme activities: RNA-dependent DNA polymerase, DNA-dependent DNA polymerase, low RNase H activity, no endonuclease activity.
Recommended reaction temperature: +42 to +55°C
Substrates: Incorporates dNTP, ddNTP, dUTP, and various labeled or modified nucleotides.
Divalent ion requirement: Mg²⁺", "Name": "Properties" } ] } } ] }