Reverse transcribe difficult templates. The high thermostability of NxtScript allows reactions up to +60°C to overcome RNA secondary structures (e.g., in GC-rich templates).
Achieve high sensitivity. NxtScript lacks RNase H activity. This results in higher cDNA yields.
Stay specific. Make use of the wide temperature activity range of NxtScript and reverse transcribe at the temperature that is optimal for your RNA target.
Convenience. Apply this enzyme directly to your reaction mix without the need for dilution
"Value": "Appearance: White cap, clear colorless solution Function test in qPCR (human reference cDNA G6PDH/β2M fragments): Corresponds to reference Stability: At -15 to -25°C wihtin specification range for 40 months.",
"Value": "NxtScript reverse transcriptase is highly thermostable and allows higher temperatures for reverse transcription, thus providing excellent results for difficult RNA targets. Enzyme activities: RNA-dependent DNA polymerase, DNA-dependent DNA polymerase, low RNase H activity, no endonuclease activity. Recommended reaction temperature: +42 to +55°C Substrates: Incorporates dNTP, ddNTP, dUTP, and various labeled or modified nucleotides. Divalent ion requirement: Mg2+",
NxtScript Reverse Transcriptase, 85 U/μl
mutant from Moloney Murine Leukemia Virus, expressed in E. coli