{
"ProductData": {
"ID": "3.6.14.3.11.2",
"ProductType": "Others",
"BrandName": "NxtScript Reverse Transcriptase, 85 U/μl",
"ProductNameAddition": "mutant from Moloney Murine Leukemia Virus, expressed in
E. coli",
"RegulatoryDisclaimer1": "For further processing only.",
"DisclaimerGroup1": "",
"RegulatoryDisclaimer2": null,
"DisclaimerGroup2": null,
"RegulatoryDisclaimer3": null,
"SampleType": [
],
"LicenseDisclaimers": [
],
"RelatedLinks": "",
"Clone": "",
"ControlTissue": [
""
],
"ISOtypes": "",
"Species": [
""
],
"StainLocalization": [
""
],
"ProductNameGlobal": "NxtScript Reverse Transcriptase 85 U uL"
},
"ProductImageDetails": {
"ImagePath": "https://pim-media.roche.com/Images/CB_IMAGE.png",
"ImageType": "Image main"
},
"Product2Taxonomy": {
"Product2TaxonomyReferences": [
{
"StructureSystemIdentifier": "OWP_Family",
"StructureSystemName": "Product Families",
"StructureNodeStatus": "valid",
"NodeName": "NxtScript",
"NodeID": "520"
},
{
"StructureSystemIdentifier": "OWP_Product_Types",
"StructureSystemName": "Product Types",
"StructureNodeStatus": "valid",
"NodeName": "Reverse Transcriptases",
"NodeID": "70-047-00"
},
{
"StructureSystemIdentifier": "Product_Grouping",
"StructureSystemName": "Product Grouping",
"StructureNodeStatus": "valid",
"NodeName": "Molecular Raw Materials",
"NodeID": "02-0136"
},
{
"StructureSystemIdentifier": "OWP_Organization",
"StructureSystemName": "Organization",
"StructureNodeStatus": "valid",
"NodeName": "CustomBiotech and LifeScience",
"NodeID": "01-01-01-07-00"
},
{
"StructureSystemIdentifier": "Product_Grouping",
"StructureSystemName": "Product Grouping",
"StructureNodeStatus": "valid",
"NodeName": "Enzymes",
"NodeID": "02-0014"
},
{
"StructureSystemIdentifier": "OWP_Techniques",
"StructureSystemName": "Techniques",
"StructureNodeStatus": "valid",
"NodeName": "RT-PCR/qRT-PCR",
"NodeID": "300-00"
},
{
"StructureSystemIdentifier": "Product_Solutions",
"StructureSystemName": "Product Solutions",
"StructureNodeStatus": "valid",
"NodeName": "CustomBiotech",
"NodeID": "520"
},
{
"StructureSystemIdentifier": "Lab_Type",
"StructureSystemName": "Lab Types",
"StructureNodeStatus": "invalid",
"NodeName": "",
"NodeID": "180-00"
},
{
"StructureSystemIdentifier": "Applications",
"StructureSystemName": "Applications",
"StructureNodeStatus": "invalid",
"NodeName": "",
"NodeID": "99-00-00"
},
{
"StructureSystemIdentifier": "Disease_Areas",
"StructureSystemName": "Disease Areas",
"StructureNodeStatus": "invalid",
"NodeName": "",
"NodeID": "99-00-00"
},
{
"StructureSystemIdentifier": "Pathogens",
"StructureSystemName": "Pathogens",
"StructureNodeStatus": "invalid",
"NodeName": "",
"NodeID": "99-00-00"
},
{
"StructureSystemIdentifier": "Health_Topics",
"StructureSystemName": "Health Topics",
"StructureNodeStatus": "invalid",
"NodeName": "",
"NodeID": "99-00-00"
}
]
},
"Product2Materials": {
"P2MaterialReferences": [
{
"MaterialNum": "07371527103",
"MaterialDescription": "NxtScript RT, 85 U/ul 80 ul",
"RegisteredProductName": "NxtScript Reverse Transcriptase, 85 U/uL",
"GTIN": "",
"ProductCategoryText": "Reagents, kits",
"OldMaterialNumber": "",
"PackSizePIM360": "80 μL",
"PackSizeDescPIM360": "85 U/μL",
"MaterialAnnotation": "CustomBiotech product. Please contact your
local representative.",
"ReadyForUse": "",
"OrderInformation": "Will be supplied as \"NxtScript RT, 85 U/μL, 80 μL\". Unit of measure is \"piece\"."
}
]
},
"Product2Products": {
"Product2ProductReference": [
{
"ProductID": "3.5.9.5.2.11",
"BrandName": "NxtScript DNA Master",
"ProductNameAddition": "5x concentrated",
"ReferenceType": "Reagent",
"Classification": [
{
"IdentifierofStructureSystem": "OWP_Family",
"NameofStructureSystem": "Product Families",
"StructureNodeID": "520",
"StructureGroupPath": "NxtScript",
"StructureGroupName": "NxtScript",
"StructureNodeStatus": "valid"
},
{
"IdentifierofStructureSystem": "OWP_Product_Types",
"NameofStructureSystem": "Product Types",
"StructureNodeID": "70-012-00",
"StructureGroupPath": "Raw Materials->DNA Master Mixes",
"StructureGroupName": "DNA Master Mixes",
"StructureNodeStatus": "valid"
},
{
"IdentifierofStructureSystem": "Product_Grouping",
"NameofStructureSystem": "Product Grouping",
"StructureNodeID": "02-0136",
"StructureGroupPath": "CustomBiotech->Molecular Raw Materials",
"StructureGroupName": "Molecular Raw Materials",
"StructureNodeStatus": "valid"
},
{
"IdentifierofStructureSystem": "OWP_Organization",
"NameofStructureSystem": "Organization",
"StructureNodeID": "01-01-01-07-00",
"StructureGroupPath": "Roche Diagnostics Solution (RDS)->Customer Areas->Core lab->CustomBiotech and LifeScience",
"StructureGroupName": "CustomBiotech and LifeScience",
"StructureNodeStatus": "valid"
},
{
"IdentifierofStructureSystem": "OWP_Techniques",
"NameofStructureSystem": "Techniques",
"StructureNodeID": "210-00",
"StructureGroupPath": "PCR/qPCR",
"StructureGroupName": "PCR/qPCR",
"StructureNodeStatus": "valid"
},
{
"IdentifierofStructureSystem": "Product_Solutions",
"NameofStructureSystem": "Product Solutions",
"StructureNodeID": "520",
"StructureGroupPath": "CustomBiotech",
"StructureGroupName": "CustomBiotech",
"StructureNodeStatus": "valid"
},
{
"IdentifierofStructureSystem": "Lab_Type",
"NameofStructureSystem": "Lab Types",
"StructureNodeID": "180-00",
"StructureGroupPath": "",
"StructureGroupName": "",
"StructureNodeStatus": "invalid"
},
{
"IdentifierofStructureSystem": "Applications",
"NameofStructureSystem": "Applications",
"StructureNodeID": "99-00-00",
"StructureGroupPath": "",
"StructureGroupName": "",
"StructureNodeStatus": "invalid"
},
{
"IdentifierofStructureSystem": "Disease_Areas",
"NameofStructureSystem": "Disease Areas",
"StructureNodeID": "99-00-00",
"StructureGroupPath": "",
"StructureGroupName": "",
"StructureNodeStatus": "invalid"
},
{
"IdentifierofStructureSystem": "Pathogens",
"NameofStructureSystem": "Pathogens",
"StructureNodeID": "99-00-00",
"StructureGroupPath": "",
"StructureGroupName": "",
"StructureNodeStatus": "invalid"
},
{
"IdentifierofStructureSystem": "Health_Topics",
"NameofStructureSystem": "Health Topics",
"StructureNodeID": "99-00-00",
"StructureGroupPath": "",
"StructureGroupName": "",
"StructureNodeStatus": "invalid"
}
]
}
]
},
"ProductSpec": [
{
"ProductSpecVariant": {
"Chapters": [
{
"Language": "en",
"Value": "Will be supplied as \"NxtScript RT, 85 U/μL, 80 μL\". Unit of measure is \"piece\".",
"Country": "XG",
"Code": "CB - Order Information",
"Name": "CB - Order Information"
},
{
"Language": "en",
"Value": "M-MLV Reverse Transcriptase mutant designed for high thermostability and comparable performance to the market leader.",
"Country": "XG",
"Code": "Positioning",
"Name": "Positioning"
},
{
"Language": "en",
"Value": "Use NxtScript RT for synthesis of cDNA from total RNA or mRNA for:
- One-step and two-step RT-PCR applications
- RT-PCR for detection of viral targets
- RT-PCR for detection of mRNA targets, such as cancer biomarkers
- TMA and NASBA nucleic acid amplification methods
- Generation of full-length cDNA libraries
- Rapid amplification of cDNA ends (RACE)
",
"Country": "XG",
"Code": "Applications",
"Name": "Applications"
},
{
"Language": "en",
"Value": "
- Reverse transcribe difficult templates.
The high thermostability of NxtScript allows reactions up to +60°C to overcome RNA secondary structures (e.g., in GC-rich templates). - Achieve high sensitivity.
NxtScript lacks RNase H activity. This results in higher cDNA yields. - Stay specific.
Make use of the wide temperature activity range of NxtScript and reverse transcribe at the temperature that is optimal for your RNA target. - Convenience.
Apply this enzyme directly to your reaction mix without the need for dilution
",
"Country": "XG",
"Code": "Benefits",
"Name": "Benefits"
},
{
"Language": "en",
"Value": "
Appearance: White cap, clear colorless solution
Function test in qPCR (human reference cDNA G6PDH/β2M fragments): Corresponds to reference
Stability: At -15 to -25°C wihtin specification range for 40 months.",
"Country": "XG",
"Code": "Specification",
"Name": "Specification"
},
{
"Language": "en",
"Value": "NxtScript reverse transcriptase is highly thermostable and allows higher temperatures for reverse transcription, thus providing excellent results for difficult RNA targets.
Enzyme activities: RNA-dependent DNA polymerase, DNA-dependent DNA polymerase, low RNase H activity, no endonuclease activity.
Recommended reaction temperature: +42 to +55°C
Substrates: Incorporates dNTP, ddNTP, dUTP, and various labeled or modified nucleotides.
Divalent ion requirement: Mg
2+",
"Country": "XG",
"Code": "Properties",
"Name": "Properties"
}
]
}
}
]
}