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Always run a negative control with the samples. To prepare a negative control, replace the template DNA or RNA with Water, PCR Grade*.",
"Name": "Control Reactions"
},
{
"Language": "en",
"Value": "
Primer concentration
Use PCR primers at a final concentration of 0.2 to 0.5 μM. The recommended concentration is 0.5 μM each.
Always use equimolar primer concentrations.
",
"Name": "Primers"
},
{
"Language": "en",
"Value": "NxtScript DNA Master is a 5x concentrated, ready-to-use, one component hot start PCR mix, containing AptaTaq DNA Polymerase in an optimized concentration for multiplex qPCR or qRT-PCR. It uses aptamer-mediated reversible hot start technology for specific priming and fast PCR. The mix contains dNTP mix with dUTP for prevention of DNA contamination by PCR carryover by pretreatment with Uracil-DNA Glycosylase.",
"Name": "Product Description"
},
{
"Language": "en",
"Value": "As a starting point, use a probe concentration of 0.25 μM each. However, suitable concentrations range from 0.05 to 0.5 μM.
The optimal probe concentration is the lowest concentration that results in the lowest quantification cycle value (Cq) and adequate fluorescence dynamics for a given target concentration.
To ensure efficient probe cleavage, the Tm of the hydrolysis probe should be higher than the Tm of the primers.
",
"Name": "Probe"
},
{
"Language": "en",
"Value": "Each lot of NxtScript DNA Master is tested to meet specifications of the qPCR using a duplex qPCR assay on the LightCycler® 480 Instrument II*.",
"Name": "Quality Control"
},
{
"Language": "en",
"Value": "Will be supplied as \"NxtScript DNA Master, 5x, 5 mL\". Unit of measure is \"piece\".",
"Name": "CB - Order Information"
},
{
"Language": "en",
"Value": "Use any DNA suitable for qPCR or template RNA, such as total RNA or mRNA suitable for RT-PCR in terms of purity, concentration, and absence of inhibitors. RNA and DNA purified from whole blood, plasma, serum, swabs, stool, and urine were tested successfully with NxtScript DNA Master.",
"Name": "Sample Materials"
},
{
"Language": "en",
"Value": "Use NxtScript Reverse Transcriptase (07051166103) in combination with NxtScript DNA Master to run highly sensitive qRT-PCR reactions to detect RNA pathogens.",
"Name": "Applications"
},
{
"Language": "en",
"Value": "Reversible hot start DNA master mix for multiplexing and as DNA master mix component in RT-PCR.",
"Name": "Positioning"
},
{
"Language": "en",
"Value": "
Save time. Take advantage of the aptamer technology and run a fast PCR protocol.
Achieve high sensitivity. Detect low copy numbers of DNA or RNA targets with higher sensitivity using a 5x master mix concentration.
Ready for automation. Set up your reaction at room temperature.
",
"Name": "Benefits"
},
{
"Language": "en",
"Value": "NxtScript DNA Master is a sensitive and robust reaction mix for detection of DNA and RNA pathogens. It is stable at +2 to +8°C for 3 months and in its final reaction setup, for 4 hours at room temperature.",
"Name": "Properties"
},
{
"Language": "en",
"Value": "Appearance: Clear colorless solution Function test in qPCR (human reference cDNA G6PDH/β2M fragments): Corresponds to reference Stability: At -15 to -25°C within specification range for 37 months.",
"Name": "Specification"
}
]
}
}
]
}
