{ "ProductData": { "ID": "3.1.1.1.5.32", "ProductType": "Others", "BrandName": "N-Glycosidase F (PNGase F)", "ProductNameAddition": "N-Glycosidase F of Flavobacterium meningosepticum, recombinant from E. coli, lyophilizate", "RegulatoryDisclaimer1": "For further processing only.", "DisclaimerGroup1": "", "RegulatoryDisclaimer2": null, "DisclaimerGroup2": null, "RegulatoryDisclaimer3": null, "SampleType": [ ], "LicenseDisclaimers": [ { "Identifier": "1403506419168", "LongText": "The sale of the Product does not exhaust or grant any rights in third party patents including patents of companies of the F. Hoffmann - La Roche AG group of companies, in particular, for the use of modified antibodies obtained by using the product.", "Order": "78" } ], "RelatedLinks": "", "Clone": "", "ControlTissue": [ "" ], "ISOtypes": "", "Species": [ "" ], "StainLocalization": [ "" ], "ProductNameGlobal": "N-Glycosidase F PNGase F" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/CB_IMAGE_.png", "ImageType": "Image main" }, "Product2Taxonomy": { "Product2TaxonomyReferences": [ { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeID": "02-0052", "StructureNodeStatus": "valid", "NodeName": "In vitro Glycoengineering" }, { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "NodeID": "70-024-00", "StructureNodeStatus": "valid", "NodeName": "Glycosidases" }, { "StructureSystemIdentifier": "OWP_Organization", "StructureSystemName": "Organization", "NodeID": "01-01-01-07-00", "StructureNodeStatus": "valid", "NodeName": "CustomBiotech and LifeScience" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeID": "02-0014", "StructureNodeStatus": "valid", "NodeName": "Enzymes" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "NodeID": "520", "StructureNodeStatus": "valid", "NodeName": "CustomBiotech" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeID": "180-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeID": "99-00-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "NodeID": "999-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "NodeID": "99-00-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "NodeID": "99-00-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "NodeID": "999", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "NodeID": "99-00-00", "StructureNodeStatus": "invalid", "NodeName": "" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "06538355103", "MaterialDescription": "N-Glycosidase F, 500 U/vial", "RegisteredProductName": "N-Glycosidase F (PNGase F)", "GTIN": "", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "500 U/vial", "PackSizeDescPIM360": "Not Available", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Unit of measure is \"piece\"." } ] }, "Product2Products": { "Product2ProductReference": [ ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Name": "Product Description", "Value": "N-Glycosidase F is a recombinant, Peptide-N4-(acetyl-β-glucosaminyl)-asparagine amidase from Flavobacterium meningosepticum, expressed in E. coli, provided as a lyophilizate.", "Language": "en", "Country": "XG", "Code": "Product Description" }, { "Name": "Applications", "Value": "N-Glycosidase F can be used in glycoprotein analysis to cleave asparagine-bound N-glycans from glycoproteins, allowing glycan and protein to be analyzed separately.", "Language": "en", "Country": "XG", "Code": "Applications" }, { "Name": "CB - Order Information", "Value": "Unit of measure is \"piece\".", "Language": "en", "Country": "XG", "Code": "CB - Order Information" }, { "Name": "Positioning", "Value": "N-Glycosidase F cleaves asparagine-bound N-glycans from glycoproteins.", "Language": "en", "Country": "XG", "Code": "Positioning" }, { "Name": "Specification", "Value": "Appearance: White lyophilized substance
Solution (after dissolution in water;c = bottle / 0.25 U/mL water): Clear, colorless solution.
Activity (at 37°C; with Dabsyl-fibrin-glycopeptide, HPLC): ≥500 U/bottle. ≥50 U/mg lyophilized material.
Specific activity: ≥25 000 U/mg protein
Purity-SDS-PAGE (not reducing): ≥90 area%
Contaminants expressed as a percentage of N-Glycosidase F activity:
Endoglycosidase F: not detectable
β-N-Acetylhexosaminidase: A/17h≤0.025%
α-Fucosidase: A/17h ≤0.025%
β-Galactosidase: A/17h ≤0.025%
β-Glucosidase: A/17h ≤0.025%
α-Mannosidase: A/17h ≤0.025%
β-Mannosidase: A/17h ≤0.025%
Sialidase: A/17h≤0.025%
Proteases (casein-resorufin): A/17h ≤0.025%
Stability: At +2 to +8°C within specification range for 24 months.", "Language": "en", "Country": "XG", "Code": "Specification" } ] } } ] }

N-Glycosidase F (PNGase F)

N-Glycosidase F of Flavobacterium meningosepticum, recombinant from E. coli, lyophilizate

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