{ "ProductData": { "RelatedLinks": "", "RegulatoryDisclaimer1": "For further processing only.", "RegulatoryDisclaimer2": "", "StainLocalization": [ "" ], "ProductType": "Others", "DisclaimerGroup2": null, "ProductNameGlobal": "N-Glycosidase F PNGase F", "DisclaimerGroup1": "Non-IVD", "ControlTissue": [ "" ], "BrandName": "N-Glycosidase F (PNGase F)", "LicenseDisclaimers": [ { "Order": "78", "Identifier": "1403506419168", "LongText": "The sale of the Product does not exhaust or grant any rights in third party patents including patents of companies of the F. Hoffmann - La Roche AG group of companies, in particular, for the use of modified antibodies obtained by using the product." } ], "Isotypes": "", "RegulatoryDisclaimer3": "", "ID": "3.1.1.1.5.32", "ProductNameAddition": "N-Glycosidase F of Flavobacterium meningosepticum, recombinant from E. coli, lyophilizate", "Species": [ "" ], "Clone": "" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/CB_IMAGE.png", "ImageType": "Image main" }, "Product2Taxomony": { "Product2TaxomonyReferences": [ { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "In vitro Glycoengineering", "NodeID": "02-0052" }, { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "NodeName": "Glycosidases", "NodeID": "70-024-00" }, { "StructureSystemIdentifier": "OWP_Organization", "StructureSystemName": "Organization", "NodeName": "CustomBiotech and LifeScience", "NodeID": "01-01-01-07-00" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "Enzymes", "NodeID": "02-0014" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "NodeName": "CustomBiotech", "NodeID": "520" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeName": "Manufacturing", "NodeID": "180-00" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "NodeName": "NOT APPLICABLE", "NodeID": "999-00" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "NodeName": "NOT APPLICABLE", "NodeID": "999" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "06538355103", "MaterialDescription": "N-Glycosidase F, 500 U/vial", "RegisteredProductName": "N-Glycosidase F (PNGase F)", "GTIN": "", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "500 U/vial", "PackSizeDescPIM360": "", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Unit of measure is \"piece\"." } ] }, "Product2Products": { "Product2ProductReference": [ ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Language": "en", "Value": "N-Glycosidase F is a recombinant, Peptide-N4-(acetyl-β-glucosaminyl)-asparagine amidase from Flavobacterium meningosepticum, expressed in E. coli, provided as a lyophilizate.", "Name": "Product Description" }, { "Language": "en", "Value": "N-Glycosidase F can be used in glycoprotein analysis to cleave asparagine-bound N-glycans from glycoproteins, allowing glycan and protein to be analyzed separately.", "Name": "Applications" }, { "Language": "en", "Value": "Unit of measure is \"piece\".", "Name": "CB - Order Information" }, { "Language": "en", "Value": "N-Glycosidase F cleaves asparagine-bound N-glycans from glycoproteins.", "Name": "Positioning" }, { "Language": "en", "Value": "Appearance: White lyophilized substance
Solution (after dissolution in water;c = bottle / 0.25 U/mL water): Clear, colorless solution.
Activity (at 37°C; with Dabsyl-fibrin-glycopeptide, HPLC): ≥500 U/bottle. ≥50 U/mg lyophilized material.
Specific activity: ≥25 000 U/mg protein
Purity-SDS-PAGE (not reducing): ≥90 area%
Contaminants expressed as a percentage of N-Glycosidase F activity:
Endoglycosidase F: not detectable
β-N-Acetylhexosaminidase: A/17h≤0.025%
α-Fucosidase: A/17h ≤0.025%
β-Galactosidase: A/17h ≤0.025%
β-Glucosidase: A/17h ≤0.025%
α-Mannosidase: A/17h ≤0.025%
β-Mannosidase: A/17h ≤0.025%
Sialidase: A/17h≤0.025%
Proteases (casein-resorufin): A/17h ≤0.025%
Stability: At +2 to +8°C within specification range for 24 months.", "Name": "Specification" } ] } } ] }

N-Glycosidase F (PNGase F)

N-Glycosidase F of Flavobacterium meningosepticum, recombinant from E. coli, lyophilizate

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