{ "ProductData": { "RelatedLinks": "", "RegulatoryDisclaimer1": "For further processing only.", "RegulatoryDisclaimer2": "", "StainLocalization": [ "" ], "DisclaimerGroup2": null, "ProductNameGlobal": "Malate Dehydrogenase IFCC Quality solution", "DisclaimerGroup1": "Other - Non IVD", "ControlTissue": [ "" ], "BrandName": "Malate Dehydrogenase, IFCC Quality", "LicenseDisclaimers": [ ], "Isotypes": "", "RegulatoryDisclaimer3": "", "ID": "3.1.1.4.18.8", "ProductNameAddition": "from pig heart, solution", "Species": [ "" ], "Clone": "" }, "ProductSpec": { "ProductSpecVariant": { "Chapters": [ { "Language": "English", "Value": "Will be supplied as \"MDH, IFCC-quality, Pig Heart (Mitochon.)\". Unit of measure is \"MU\".", "Name": "CB - Order Information" }, { "Language": "English", "Value": "Nomenclature: L-malate:NAD⁺ oxidoreductase
Molecular weight: 70 kD
Isoelectric point: 6.1-6.4
Michaelis constants (Phosphate buffer, 95 mmol/L, pH 8.3, +25°C):
L-malate: 4.0 x 10^-4 mol/L
L-tartrate: 9.0 x 10^-3 mol/L
meso-tartrate: 1.2 x 10^-3 mol/L
oxaloacetate: 3.3 x 10^-5 mol/L
Inhibitors: Iodinated compounds such as iodine cyanide, thyroxine and molecular iodine, phenols, 1,10-phenanthroline, 8-hydroxyquinoline, sulfide, nicotinic acidamide, adenine, AMP, ATP; oxaloacetate (excess).
Activators: Phosphate, arsenate, Zn²⁺
pH optimum: 7.5 (see figure for product Cat. No. 10 267 155 103)
Temperature dependence: See figure for product Cat. No. 10 267 155 103
pH stability: 7.0-9.0 (see figure for product Cat. No. 10 267 155 103)
Thermal stability: Up to +40°C (see figure for product Cat. No. 10 267 155 103)
Specificity: L-configuration of malate and tartrate. NAD can be replaced by its analogs, but not by NADP.", "Name": "CB - Properties" }, { "Language": "English", "Value": "Appearance: Clear, colorless solution in glycerol (50% (v/v))
pH value: 6.5-7.5
Specific activity (+30°C, oxaloacetate): ≥900 U/mg protein
Protein (Biuret): ≥10 mg/mL
Contaminants (expressed as percentage of Malate Dehydrogenase activity):
Aspartate aminotransferase (AST/GOT): ≤0.005
Alanine aminotransferase (ALT/GPT): ≤0.005
Glutamate dehydrogenase: ≤0.003
Reagent blank for determination of aspartate aminotransferase (AST/GOT): ≤0.009 (A₃₃₄/10 minutes)
SVD free: Corresponds to specification
pH 5.5 treatment (for at minimum 30 minutes): Corresponds to specification
Stability: At +2 to +8°C within specification range for 12 months.", "Name": "CB - Specification" }, { "Language": "English", "Value": "Use Malate Dehydrogenase in diagnostic tests for the determination of aspartate aminotransferase or in applications for citric and acetic acid testing.", "Name": "Applications" }, { "Language": "English", "Value": "Dehydrogenase that catalyzes the interconversion of malate to oxaloacetate.", "Name": "Positioning" }, { "Language": "English", "Value": "Appearance: Clear, colorless solution in glycerol (50% (v/v))
pH value: 6.5-7.5
Specific activity (+30°C, oxaloacetate): ≥900 U/mg protein
Protein (Biuret): ≥10 mg/mL
Contaminants (expressed as percentage of Malate Dehydrogenase activity):
Aspartate aminotransferase (AST/GOT): ≤0.005
Alanine aminotransferase (ALT/GPT): ≤0.005
Glutamate dehydrogenase: ≤0.003
Reagent blank for determination of aspartate aminotransferase (AST/GOT): ≤0.009 (A₃₃₄/10 minutes)
SVD free: Corresponds to specification
pH 5.5 treatment (for at minimum 30 minutes): Corresponds to specification
Stability: At +2 to +8°C within specification range for 12 months.", "Name": "Specification" }, { "Language": "English", "Value": "Nomenclature: L-malate:NAD⁺ oxidoreductase
Molecular weight: 70 kD
Isoelectric point: 6.1-6.4
Michaelis constants (Phosphate buffer, 95 mmol/L, pH 8.3, +25°C):
L-malate: 4.0 x 10^-4 mol/L
L-tartrate: 9.0 x 10^-3 mol/L
meso-tartrate: 1.2 x 10^-3 mol/L
oxaloacetate: 3.3 x 10^-5 mol/L
Inhibitors: Iodinated compounds such as iodine cyanide, thyroxine and molecular iodine, phenols, 1,10-phenanthroline, 8-hydroxyquinoline, sulfide, nicotinic acidamide, adenine, AMP, ATP; oxaloacetate (excess).
Activators: Phosphate, arsenate, Zn²⁺
pH optimum: 7.5 (see figure)
Temperature dependence: See figure
pH stability: 7.0-9.0 (see figure)
Thermal stability: Up to +40°C (see figure)
Specificity: L-configuration of malate and tartrate. NAD can be replaced by its analogs, but not by NADP.", "Name": "Properties" } ] } }, "Product2Taxomony": { "Product2TaxomonyReferences": [ { "StructureSystemName": "OWP Product Solutions", "NodeName": "CustomBiotech", "StructureSystemID": null, "NodeID": "520" }, { "StructureSystemName": "Product Grouping", "NodeName": "CustomBiotech->Enzymes", "StructureSystemID": null, "NodeID": "02-0014" }, { "StructureSystemName": "OWP Product Types", "NodeName": "Raw Materials and Reagents->Dehydrogenases", "StructureSystemID": null, "NodeID": "70-014-00" }, { "StructureSystemName": "OWP Organization", "NodeName": "Roche Diagnostics Solution (RDS)->Customer Areas->Core lab->CustomBiotech and LifeScience", "StructureSystemID": null, "NodeID": "01-01-01-07-00" }, { "StructureSystemName": "Applications", "NodeName": "Enzyme Testing", "StructureSystemID": null, "NodeID": "17-00-00" }, { "StructureSystemName": "Product Grouping", "NodeName": "CustomBiotech->Clinical Chemistry Raw Materials", "StructureSystemID": null, "NodeID": "02-0011" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "10417726103", "MaterialDescription": "MDH, IFCC-quality, Pig Heart (Mitochon.)", "RegisteredProductName": "", "GTIN": "14038377155392", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "custom fill", "PackSizeDescPIM360": "", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"MDH, IFCC-quality, Pig Heart (Mitochon.)\". Unit of measure is \"MU\"." } ] }, "Product2Products": { "Product2ProductReference": [ ] } }