{ "ProductData": { "ID": "3.6.14.3.9.5", "ProductType": "Others", "BrandName": "M-MLV Reverse Transcriptase, GMP Grade", "ProductNameAddition": "from Moloney Murine Leukemia Virus, expressed in E. coli", "RegulatoryDisclaimer1": "For further processing only.", "DisclaimerGroup1": "", "RegulatoryDisclaimer2": null, "DisclaimerGroup2": null, "RegulatoryDisclaimer3": "For customers in the European Economic Area: Contains SVHC: octyl/nonylphenol ethoxylates. For further processing on its own or in a mixture as part of an IVD method and under controlled conditions only – acc. to Art. 56 (3) and 3 no. 23 REACH Regulation.", "SampleType": [ ], "LicenseDisclaimers": [ ], "RelatedLinks": "", "Clone": "", "ControlTissue": [ "" ], "ISOtypes": "", "Species": [ "" ], "StainLocalization": [ "" ], "ProductNameGlobal": "M-MLV Reverse Transcriptase GMP Grade" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/CB_IMAGE_.png", "ImageType": "Image main" }, "Product2Taxonomy": { "Product2TaxonomyReferences": [ { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "NodeID": "70-047-00", "StructureNodeStatus": "valid", "NodeName": "Reverse Transcriptases" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeID": "02-0136", "StructureNodeStatus": "valid", "NodeName": "Molecular Raw Materials" }, { "StructureSystemIdentifier": "OWP_Organization", "StructureSystemName": "Organization", "NodeID": "01-01-01-07-00", "StructureNodeStatus": "valid", "NodeName": "CustomBiotech and LifeScience" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeID": "02-0014", "StructureNodeStatus": "valid", "NodeName": "Enzymes" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "NodeID": "300-00", "StructureNodeStatus": "valid", "NodeName": "RT-PCR/qRT-PCR" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "NodeID": "520", "StructureNodeStatus": "valid", "NodeName": "CustomBiotech" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeID": "180-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeID": "99-00-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "NodeID": "99-00-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "NodeID": "99-00-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "NodeID": "999", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "NodeID": "99-00-00", "StructureNodeStatus": "invalid", "NodeName": "" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "04707486103", "MaterialDescription": "M-MLV RT GMP Grade, 200 KU", "RegisteredProductName": "M-mLV Reverse Transcriptase, GMP Grade", "GTIN": "07613336132661", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "200 kU", "PackSizeDescPIM360": "Not Available", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"M-MLV RT GMP Grade, 200 KU\". Unit of measure is \"piece\".
The enzyme is supplied without reaction buffer." } ] }, "Product2Products": { "Product2ProductReference": [ ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Name": "CB - Order Information", "Value": "Will be supplied as \"M-MLV RT GMP Grade, 200 KU\". Unit of measure is \"piece\".
The enzyme is supplied without reaction buffer.", "Language": "en", "Country": "XG", "Code": "CB - Order Information" }, { "Name": "Applications", "Value": "Use M-MLV Reverse Transcriptase for synthesis of cDNA from total RNA or mRNA for:

• Two-step RT-PCR applications for amplification from RNA targets
• RT-PCR for detection of viral RNA
• TMA and NASBA nucleic acid amplification methods
• Synthesis of full-length cDNA for libraries or cloning
• Rapid amplification of cDNA end (RACE)
• Manufacture of amplification mixtures for applications with regulatory requirements (e.g., in vitro diagnostics, quality control)

", "Language": "en", "Country": "XG", "Code": "Applications" }, { "Name": "Quality Control", "Value": "Manufactured under GMP (Good Manufacturing Practice) regulations.", "Language": "en", "Country": "XG", "Code": "Quality Control" }, { "Name": "Positioning", "Value": "M-MLV Reverse Transcriptase for preparation of full-length cDNA with high efficiency.", "Language": "en", "Country": "XG", "Code": "Positioning" }, { "Name": "Properties", "Value": "M-MLV Reverse Transcriptase, GMP Grade, is highly processive and generates full length cDNA with high efficiency. It has a lower RNase H activity than AMV Reverse Transcriptase and lacks endonuclease activity.
CAS Number: 9068-38-6
Enzyme activities: RNA-dependent DNA polymerase, DNA-dependent DNA polymerase, low RNase H activity, no endonuclease activity
Recommended reaction temperature: +37°C
Substrates: Incorporates dNTP, ddNTP, dUPT, various labeled or modified nucleotides
Divalent ion requirement: Mg²⁺", "Language": "en", "Country": "XG", "Code": "Properties" }, { "Name": "Specification", "Value": "Appearance: Clear, colorless solution
Storage buffer: Tris/HCl, 25 mmol/L; NaCl, 100 mmol/L; DTT, 10 mmol/L; EDTA, 0.1 mmol/L; Triton X-100, 0.01% (v/v); glycerol, 50% (v/v); pH approximately 7.5
Volume activity: 200-300 U/μL
Specific activity: ≥100 kU/mg protein
Unit definition: One unit M-MLV Reverse Transcriptase, GMP Grade, is defined as the amount of enzyme which incorporates 1 nmol of [³H]TMP into an acid insoluble product in 10 minutes at +37°C with poly(A)x(dT)₁₅ as substrate.
Purity (SDS PAGE): ≥90%
Unspecific endonucleases (MWM III DNA): Not detectable in up to 100 U after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 100 U after 16 hours incubation at +37°C.
Ribonucleases (MS2 RNA): Not detectable in up to 200 U after 1 hour incubation at +37°C.
Bioburden: ≤50 CFU/mL
Animal-derived additives: None
Stability: At -15 to -25°C within specification range for 12 months.", "Language": "en", "Country": "XG", "Code": "Specification" } ] } } ] }