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{
"ProductData": {
"RelatedLinks": "",
"RegulatoryDisclaimer1": "",
"RegulatoryDisclaimer2": "",
"StainLocalization": [
""
],
"ProductType": "Others",
"DisclaimerGroup2": null,
"ProductNameGlobal": "M-MLV Reverse Transcriptase GMP Grade",
"DisclaimerGroup1": null,
"ControlTissue": [
""
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"BrandName": "M-MLV Reverse Transcriptase, GMP Grade",
"LicenseDisclaimers": [
],
"Isotypes": "",
"RegulatoryDisclaimer3": "For customers in the European Economic Area: Contains SVHC: octyl/nonylphenol ethoxylates. For further processing on its own or in a mixture as part of an IVD method and under controlled conditions only – acc. to Art. 56 (3) and 3 no. 23 REACH Regulation.",
"ID": "3.6.14.3.9.5",
"ProductNameAddition": "from Moloney Murine Leukemia Virus, expressed in E. coli",
"Species": [
""
],
"Clone": ""
},
"ProductImageDetails": {
"ImagePath": "https://pim-media.roche.com/Images/CB_IMAGE.png",
"ImageType": "Image main"
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"StructureSystemIdentifier": "OWP_Product_Solutions",
"StructureSystemName": "OWP Product Solutions",
"NodeName": "CustomBiotech",
"NodeID": "520"
},
{
"StructureSystemIdentifier": "OWP_Product_Types",
"StructureSystemName": "OWP Product Types",
"NodeName": "Raw Materials->Reverse Transcriptases",
"NodeID": "70-047-00"
},
{
"StructureSystemIdentifier": "Product_Grouping",
"StructureSystemName": "Product Grouping",
"NodeName": "CustomBiotech->Molecular Raw Materials",
"NodeID": "02-0136"
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{
"StructureSystemIdentifier": "OWP_Organization",
"StructureSystemName": "OWP Organization",
"NodeName": "Roche Diagnostics Solution (RDS)->Customer Areas->Core lab->CustomBiotech and LifeScience",
"NodeID": "01-01-01-07-00"
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"StructureSystemIdentifier": "Product_Grouping",
"StructureSystemName": "Product Grouping",
"NodeName": "CustomBiotech->Enzymes",
"NodeID": "02-0014"
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"StructureSystemIdentifier": "OWP_Techniques",
"StructureSystemName": "OWP Techniques",
"NodeName": "RT-PCR/qRT-PCR",
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"Product2Materials": {
"P2MaterialReferences": [
{
"MaterialNum": "04707486103",
"MaterialDescription": "M-MLV RT GMP Grade, 200 KU",
"RegisteredProductName": "M-mLV Reverse Transcriptase, GMP Grade",
"GTIN": "07613336132661",
"ProductCategoryText": "Reagents, kits",
"OldMaterialNumber": "",
"PackSizePIM360": "200 kU",
"PackSizeDescPIM360": "",
"MaterialAnnotation": "CustomBiotech product. Please contact your local representative.",
"ReadyForUse": "",
"OrderInformation": "Will be supplied as \"M-MLV RT GMP Grade, 200 KU\". Unit of measure is \"piece\". The enzyme is supplied without reaction buffer."
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"ProductSpec": [
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"Chapters": [
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"Language": "en",
"Value": "Will be supplied as \"M-MLV RT GMP Grade, 200 KU\". Unit of measure is \"piece\". The enzyme is supplied without reaction buffer.",
"Name": "CB - Order Information"
},
{
"Language": "en",
"Value": "Use M-MLV Reverse Transcriptase for synthesis of cDNA from total RNA or mRNA for:
Two-step RT-PCR applications for amplification from RNA targets
RT-PCR for detection of viral RNA
TMA and NASBA nucleic acid amplification methods
Synthesis of full-length cDNA for libraries or cloning
Rapid amplification of cDNA end (RACE)
Manufacture of amplification mixtures for applications with regulatory requirements (e.g.,in vitro diagnostics, quality control)
",
"Name": "Applications"
},
{
"Language": "en",
"Value": "Manufactured under GMP (Good Manufacturing Practice) regulations.",
"Name": "Quality Control"
},
{
"Language": "en",
"Value": "M-MLV Reverse Transcriptase for preparation of full-length cDNA with high efficiency.",
"Name": "Positioning"
},
{
"Language": "en",
"Value": "M-MLV Reverse Transcriptase, GMP Grade, is highly processive and generates full length cDNA with high efficiency. It has a lower RNase H activity than AMV Reverse Transcriptase and lacks endonuclease activity. CAS Number: 9068-38-6 Enzyme activities: RNA-dependent DNA polymerase, DNA-dependent DNA polymerase, low RNase H activity, no endonuclease activity Recommended reaction temperature: +37°C Substrates: Incorporates dNTP, ddNTP, dUPT, various labeled or modified nucleotides Divalent ion requirement: Mg2+",
"Name": "Properties"
},
{
"Language": "en",
"Value": "Appearance: Clear, colorless solution Storage buffer: Tris/HCl, 25 mmol/L; NaCl, 100 mmol/L; DTT, 10 mmol/L; EDTA, 0.1 mmol/L; Triton X-100, 0.01% (v/v); glycerol, 50% (v/v); pH approximately 7.5 Volume activity: 200-300 U/μL Specific activity: ≥100 kU/mg protein Unit definition: One unit M-MLV Reverse Transcriptase, GMP Grade, is defined as the amount of enzyme which incorporates 1 nmol of [3H]TMP into an acid insoluble product in 10 minutes at +37°C with poly(A)x(dT)15 as substrate. Purity (SDS PAGE): ≥90% Unspecific endonucleases (MWM III DNA): Not detectable in up to 100 U after 16 hours incubation at +37°C. Nicking activity (pBR322 DNA): Not detectable in up to 100 U after 16 hours incubation at +37°C. Ribonucleases (MS2 RNA): Not detectable in up to 200 U after 1 hour incubation at +37°C. Bioburden: ≤50 CFU/mL Animal-derived additives: None Stability: At -15 to -25°C within specification range for 12 months.",
"Name": "Specification"
}
]
}
}
]
}
M-MLV Reverse Transcriptase, GMP Grade
from Moloney Murine Leukemia Virus, expressed in E. coli