{ "ProductData": { "RelatedLinks": "", "RegulatoryDisclaimer1": "", "RegulatoryDisclaimer2": "", "StainLocalization": [ "" ], "DisclaimerGroup2": null, "ProductNameGlobal": "M-MLV Reverse Transcriptase GMP Grade", "DisclaimerGroup1": null, "ControlTissue": [ "" ], "BrandName": "M-MLV Reverse Transcriptase, GMP Grade", "LicenseDisclaimers": [ ], "Isotypes": "", "RegulatoryDisclaimer3": "For customers in the European Economic Area: Contains SVHC: octyl/nonylphenol ethoxylates. For further processing on its own or in a mixture as part of an IVD method and under controlled conditions only – acc. to Art. 56 (3) and 3 no. 23 REACH Regulation.", "ID": "3.6.14.3.9.5", "ProductNameAddition": "from Moloney Murine Leukemia Virus, expressed in E. coli", "Species": [ "" ], "Clone": "" }, "ProductSpec": { "ProductSpecVariant": { "Chapters": [ { "Language": "English", "Value": "Will be supplied as \"M-MLV RT GMP Grade, 200 KU\". Unit of measure is \"piece\".
The enzyme is supplied without reaction buffer.", "Name": "CB - Order Information" }, { "Language": "English", "Value": "Use M-MLV Reverse Transcriptase for synthesis of cDNA from total RNA or mRNA for:

• Two-step RT-PCR applications for amplification from RNA targets
• RT-PCR for detection of viral RNA
• TMA and NASBA nucleic acid amplification methods
• Synthesis of full-length cDNA for libraries or cloning
• Rapid amplification of cDNA end (RACE)
• Manufacture of amplification mixtures for applications with regulatory requirements (e.g., in vitro diagnostics, quality control)

", "Name": "Applications" }, { "Language": "English", "Value": "Appearance: Clear, colorless solution
Storage buffer: Tris/HCl, 25 mmol/L; NaCl, 100 mmol/L; DTT, 10 mmol/L; EDTA, 0.1 mmol/L; Triton X-100, 0.01% (v/v); glycerol, 50% (v/v); pH approximately 7.5
Volume activity: 200-300 U/μL
Specific activity: ≥100 kU/mg protein
Unit definition: One unit M-MLV Reverse Transcriptase, GMP Grade, is defined as the amount of enzyme which incorporates 1 nmol of [³H]TMP into an acid insoluble product in 10 minutes at +37°C with poly(A)x(dT)₁₅ as substrate.
Purity (SDS PAGE): ≥90%
Unspecific endonucleases (MWM III DNA): Not detectable in up to 100 U after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 100 U after 16 hours incubation at +37°C.
Ribonucleases (MS2 RNA): Not detectable in up to 200 U after 1 hour incubation at +37°C.
Bioburden: ≤50 CFU/mL
Animal-derived additives: None
Stability: At -15 to -25°C within specification range for 12 months.", "Name": "CB - Specification" }, { "Language": "English", "Value": "M-MLV Reverse Transcriptase, GMP Grade, is highly processive and generates full length cDNA with high efficiency. It has a lower RNase H activity than AMV Reverse Transcriptase and lacks endonuclease activity.
Enzyme activities: RNA-dependent DNA polymerase, DNA-dependent DNA polymerase, low RNase H activity, no endonuclease activity
Recommended reaction temperature: +37°C
Substrates: Incorporates dNTP, ddNTP, dUPT, various labeled or modified nucleotides
Divalent ion requirement: Mg²⁺", "Name": "CB - Properties" }, { "Language": "English", "Value": "Manufactured under GMP (Good Manufacturing Practice) regulations.", "Name": "Quality Control" }, { "Language": "English", "Value": "M-MLV Reverse Transcriptase for preparation of full-length cDNA with high efficiency.", "Name": "Positioning" }, { "Language": "English", "Value": "M-MLV Reverse Transcriptase, GMP Grade, is highly processive and generates full length cDNA with high efficiency. It has a lower RNase H activity than AMV Reverse Transcriptase and lacks endonuclease activity.
Enzyme activities: RNA-dependent DNA polymerase, DNA-dependent DNA polymerase, low RNase H activity, no endonuclease activity
Recommended reaction temperature: +37°C
Substrates: Incorporates dNTP, ddNTP, dUPT, various labeled or modified nucleotides
Divalent ion requirement: Mg²⁺", "Name": "Properties" }, { "Language": "English", "Value": "Appearance: Clear, colorless solution
Storage buffer: Tris/HCl, 25 mmol/L; NaCl, 100 mmol/L; DTT, 10 mmol/L; EDTA, 0.1 mmol/L; Triton X-100, 0.01% (v/v); glycerol, 50% (v/v); pH approximately 7.5
Volume activity: 200-300 U/μL
Specific activity: ≥100 kU/mg protein
Unit definition: One unit M-MLV Reverse Transcriptase, GMP Grade, is defined as the amount of enzyme which incorporates 1 nmol of [³H]TMP into an acid insoluble product in 10 minutes at +37°C with poly(A)x(dT)₁₅ as substrate.
Purity (SDS PAGE): ≥90%
Unspecific endonucleases (MWM III DNA): Not detectable in up to 100 U after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 100 U after 16 hours incubation at +37°C.
Ribonucleases (MS2 RNA): Not detectable in up to 200 U after 1 hour incubation at +37°C.
Bioburden: ≤50 CFU/mL
Animal-derived additives: None
Stability: At -15 to -25°C within specification range for 12 months.", "Name": "Specification" } ] } }, "Product2Taxomony": { "Product2TaxomonyReferences": [ { "StructureSystemName": "OWP Product Solutions", "NodeName": "CustomBiotech", "StructureSystemID": null, "NodeID": "520" }, { "StructureSystemName": "OWP Product Types", "NodeName": "Raw Materials and Reagents->Reverse Transcriptases", "StructureSystemID": null, "NodeID": "70-047-00" }, { "StructureSystemName": "Product Grouping", "NodeName": "CustomBiotech->Molecular Raw Materials", "StructureSystemID": null, "NodeID": "02-0136" }, { "StructureSystemName": "OWP Organization", "NodeName": "Roche Diagnostics Solution (RDS)->Customer Areas->Core lab->CustomBiotech and LifeScience", "StructureSystemID": null, "NodeID": "01-01-01-07-00" }, { "StructureSystemName": "Product Grouping", "NodeName": "CustomBiotech->Enzymes", "StructureSystemID": null, "NodeID": "02-0014" }, { "StructureSystemName": "OWP Techniques", "NodeName": "RT-PCR/qRT-PCR", "StructureSystemID": null, "NodeID": "300-00" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "04707486103", "MaterialDescription": "M-MLV RT GMP Grade, 200 KU", "RegisteredProductName": "", "GTIN": "07613336132661", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "200 kU", "PackSizeDescPIM360": "", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"M-MLV RT GMP Grade, 200 KU\". Unit of measure is \"piece\".
The enzyme is supplied without reaction buffer." } ] }, "Product2Products": { "Product2ProductReference": [ ] } }