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"BrandName": "M-MLV Reverse Transcriptase, GMP Grade",
"ProductNameAddition": "from Moloney Murine Leukemia Virus, expressed in
E. coli",
"RegulatoryDisclaimer1": "For further processing only.",
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"RegulatoryDisclaimer2": null,
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"RegulatoryDisclaimer3": "For customers in the European Economic Area: Contains SVHC: octyl/nonylphenol ethoxylates. For further processing on its own or in a mixture as part of an IVD method and under controlled conditions only – acc. to Art. 56 (3) and 3 no. 23 REACH Regulation.",
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"NodeName": "Reverse Transcriptases"
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"MaterialNum": "04707486103",
"MaterialDescription": "M-MLV RT GMP Grade, 200 KU",
"RegisteredProductName": "M-mLV Reverse Transcriptase, GMP Grade",
"GTIN": "07613336132661",
"ProductCategoryText": "Reagents, kits",
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"PackSizePIM360": "200 kU",
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The enzyme is supplied without reaction buffer."
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"Name": "CB - Order Information",
"Value": "Will be supplied as \"M-MLV RT GMP Grade, 200 KU\". Unit of measure is \"piece\".
The enzyme is supplied without reaction buffer.",
"Language": "en",
"Country": "XG",
"Code": "CB - Order Information"
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{
"Name": "Applications",
"Value": "Use M-MLV Reverse Transcriptase for synthesis of cDNA from total RNA or mRNA for:
- Two-step RT-PCR applications for amplification from RNA targets
- RT-PCR for detection of viral RNA
- TMA and NASBA nucleic acid amplification methods
- Synthesis of full-length cDNA for libraries or cloning
- Rapid amplification of cDNA end (RACE)
- Manufacture of amplification mixtures for applications with regulatory requirements (e.g., in vitro diagnostics, quality control)
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"Country": "XG",
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"Name": "Quality Control",
"Value": "Manufactured under GMP (Good Manufacturing Practice) regulations.",
"Language": "en",
"Country": "XG",
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"Name": "Positioning",
"Value": "M-MLV Reverse Transcriptase for preparation of full-length cDNA with high efficiency.",
"Language": "en",
"Country": "XG",
"Code": "Positioning"
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{
"Name": "Properties",
"Value": "M-MLV Reverse Transcriptase, GMP Grade, is highly processive and generates full length cDNA with high efficiency. It has a lower RNase H activity than AMV Reverse Transcriptase and lacks endonuclease activity.
CAS Number: 9068-38-6
Enzyme activities: RNA-dependent DNA polymerase, DNA-dependent DNA polymerase, low RNase H activity, no endonuclease activity
Recommended reaction temperature: +37°C
Substrates: Incorporates dNTP, ddNTP, dUPT, various labeled or modified nucleotides
Divalent ion requirement: Mg
2+",
"Language": "en",
"Country": "XG",
"Code": "Properties"
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"Name": "Specification",
"Value": "
Appearance: Clear, colorless solution
Storage buffer: Tris/HCl, 25 mmol/L; NaCl, 100 mmol/L; DTT, 10 mmol/L; EDTA, 0.1 mmol/L; Triton X-100, 0.01% (v/v); glycerol, 50% (v/v); pH approximately 7.5
Volume activity: 200-300 U/μL
Specific activity: ≥100 kU/mg protein
Unit definition: One unit M-MLV Reverse Transcriptase, GMP Grade, is defined as the amount of enzyme which incorporates 1 nmol of [
3H]TMP into an acid insoluble product in 10 minutes at +37°C with poly(A)x(dT)
15 as substrate.
Purity (SDS PAGE): ≥90%
Unspecific endonucleases (MWM III DNA): Not detectable in up to 100 U after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 100 U after 16 hours incubation at +37°C.
Ribonucleases (MS2 RNA): Not detectable in up to 200 U after 1 hour incubation at +37°C.
Bioburden: ≤50 CFU/mL
Animal-derived additives: None
Stability: At -15 to -25°C within specification range for 12 months.",
"Language": "en",
"Country": "XG",
"Code": "Specification"
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