For further processing only. Others M-MLV Reverse Transcriptase GMP Grade M-MLV Reverse Transcriptase, GMP Grade 3.6.14.3.9.5 from Moloney Murine Leukemia Virus, expressed in E. coli 04 707 486 103 4 707 486 103 04707486103 4707486103 04707486103 M-MLV RT GMP Grade, 200 KU M-mLV Reverse Transcriptase, GMP Grade 07613336132661 Reagents, kits 200 kU Not Available CustomBiotech product. Please contact your local representative. Will be supplied as "M-MLV RT GMP Grade, 200 KU". Unit of measure is "piece".The enzyme is supplied without reaction buffer. EC 2.7.7.49 en 9068-38-6 en Use M-MLV Reverse Transcriptase for synthesis of cDNA from total RNA or mRNA for: Two-step RT-PCR applications for amplification from RNA targetsRT-PCR for detection of viral RNATMA and NASBA nucleic acid amplification methodsSynthesis of full-length cDNA for libraries or cloningRapid amplification of cDNA end (RACE)Manufacture of amplification mixtures for applications with regulatory requirements (e.g., in vitro diagnostics, quality control) en M-MLV Reverse Transcriptase for preparation of full-length cDNA with high efficiency. en Appearance: Clear, colorless solutionStorage buffer: Tris/HCl, 25 mmol/L; NaCl, 100 mmol/L; DTT, 10 mmol/L; EDTA, 0.1 mmol/L; Triton X-100, 0.01% (v/v); glycerol, 50% (v/v); pH approximately 7.5Volume activity: 200-300 U/μLSpecific activity: ≥100 kU/mg proteinUnit definition: One unit M-MLV Reverse Transcriptase, GMP Grade, is defined as the amount of enzyme which incorporates 1 nmol of [3H]TMP into an acid insoluble product in 10 minutes at +37°C with poly(A)x(dT)15 as substrate.Purity (SDS PAGE): ≥90%Unspecific endonucleases (MWM III DNA): Not detectable in up to 100 U after 16 hours incubation at +37°C.Nicking activity (pBR322 DNA): Not detectable in up to 100 U after 16 hours incubation at +37°C.Ribonucleases (MS2 RNA): Not detectable in up to 200 U after 1 hour incubation at +37°C.Bioburden: ≤50 CFU/mLAnimal-derived additives: NoneStability: At -15 to -25°C within specification range for 18 months. en

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M-MLV Reverse Transcriptase, GMP Grade

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