{ "ProductData": { "ID": "3.6.14.3.9.5", "ProductType": "Others", "BrandName": "M-MLV Reverse Transcriptase, GMP Grade", "ProductNameAddition": "from Moloney Murine Leukemia Virus, expressed in E. coli", "RegulatoryDisclaimer1": "For further processing only.", "DisclaimerGroup1": "", "RegulatoryDisclaimer2": null, "DisclaimerGroup2": null, "RegulatoryDisclaimer3": "For customers in the European Economic Area: Contains SVHC: octyl/nonylphenol ethoxylates. For further processing on its own or in a mixture as part of an IVD method and under controlled conditions only – acc. to Art. 56 (3) and 3 no. 23 REACH Regulation.", "SampleType": [ ], "LicenseDisclaimers": [ ], "RelatedLinks": "", "Clone": "", "ControlTissue": [ "" ], "ISOtypes": "", "Species": [ "" ], "StainLocalization": [ "" ], "ProductNameGlobal": "M-MLV Reverse Transcriptase GMP Grade" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/CB_IMAGE_.png", "ImageType": "Image main" }, "Product2Taxonomy": { "Product2TaxonomyReferences": [ { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "NodeID": "70-047-00", "StructureNodeStatus": "valid", "NodeName": "Reverse Transcriptases" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeID": "02-0136", "StructureNodeStatus": "valid", "NodeName": "Molecular Raw Materials" }, { "StructureSystemIdentifier": "OWP_Organization", "StructureSystemName": "Organization", "NodeID": "01-01-01-07-00", "StructureNodeStatus": "valid", "NodeName": "CustomBiotech and LifeScience" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeID": "02-0014", "StructureNodeStatus": "valid", "NodeName": "Enzymes" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "NodeID": "300-00", "StructureNodeStatus": "valid", "NodeName": "RT-PCR/qRT-PCR" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "NodeID": "520", "StructureNodeStatus": "valid", "NodeName": "CustomBiotech" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeID": "180-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeID": "99-00-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "NodeID": "99-00-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "NodeID": "99-00-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "NodeID": "999", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "NodeID": "99-00-00", "StructureNodeStatus": "invalid", "NodeName": "" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "04707486103", "MaterialDescription": "M-MLV RT GMP Grade, 200 KU", "RegisteredProductName": "M-mLV Reverse Transcriptase, GMP Grade", "GTIN": "07613336132661", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "200 kU", "PackSizeDescPIM360": "Not Available", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"M-MLV RT GMP Grade, 200 KU\". Unit of measure is \"piece\".
The enzyme is supplied without reaction buffer." } ] }, "Product2Products": { "Product2ProductReference": [ ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Name": "CB - Order Information", "Value": "Will be supplied as \"M-MLV RT GMP Grade, 200 KU\". Unit of measure is \"piece\".
The enzyme is supplied without reaction buffer.", "Language": "en", "Country": "XG", "Code": "CB - Order Information" }, { "Name": "Applications", "Value": "Use M-MLV Reverse Transcriptase for synthesis of cDNA from total RNA or mRNA for:
  • Two-step RT-PCR applications for amplification from RNA targets
  • RT-PCR for detection of viral RNA
  • TMA and NASBA nucleic acid amplification methods
  • Synthesis of full-length cDNA for libraries or cloning
  • Rapid amplification of cDNA end (RACE)
  • Manufacture of amplification mixtures for applications with regulatory requirements (e.g., in vitro diagnostics, quality control)
", "Language": "en", "Country": "XG", "Code": "Applications" }, { "Name": "Quality Control", "Value": "Manufactured under GMP (Good Manufacturing Practice) regulations.", "Language": "en", "Country": "XG", "Code": "Quality Control" }, { "Name": "Positioning", "Value": "M-MLV Reverse Transcriptase for preparation of full-length cDNA with high efficiency.", "Language": "en", "Country": "XG", "Code": "Positioning" }, { "Name": "Properties", "Value": "M-MLV Reverse Transcriptase, GMP Grade, is highly processive and generates full length cDNA with high efficiency. It has a lower RNase H activity than AMV Reverse Transcriptase and lacks endonuclease activity.
CAS Number: 9068-38-6
Enzyme activities: RNA-dependent DNA polymerase, DNA-dependent DNA polymerase, low RNase H activity, no endonuclease activity
Recommended reaction temperature: +37°C
Substrates: Incorporates dNTP, ddNTP, dUPT, various labeled or modified nucleotides
Divalent ion requirement: Mg2+", "Language": "en", "Country": "XG", "Code": "Properties" }, { "Name": "Specification", "Value": "Appearance: Clear, colorless solution
Storage buffer: Tris/HCl, 25 mmol/L; NaCl, 100 mmol/L; DTT, 10 mmol/L; EDTA, 0.1 mmol/L; Triton X-100, 0.01% (v/v); glycerol, 50% (v/v); pH approximately 7.5
Volume activity: 200-300 U/μL
Specific activity: ≥100 kU/mg protein
Unit definition: One unit M-MLV Reverse Transcriptase, GMP Grade, is defined as the amount of enzyme which incorporates 1 nmol of [3H]TMP into an acid insoluble product in 10 minutes at +37°C with poly(A)x(dT)15 as substrate.
Purity (SDS PAGE): ≥90%
Unspecific endonucleases (MWM III DNA): Not detectable in up to 100 U after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 100 U after 16 hours incubation at +37°C.
Ribonucleases (MS2 RNA): Not detectable in up to 200 U after 1 hour incubation at +37°C.
Bioburden: ≤50 CFU/mL
Animal-derived additives: None
Stability: At -15 to -25°C within specification range for 12 months.", "Language": "en", "Country": "XG", "Code": "Specification" } ] } } ] }

M-MLV Reverse Transcriptase, GMP Grade

from Moloney Murine Leukemia Virus, expressed in E. coli

logo

Ordering Information

Technical documents

for certificates, method sheets (instructions for use), safety data sheets and software downloads
error errorMessage
Sorry, we couldn't find the content you are looking for
Please try again later