For life science research only. Not for use in diagnostic procedures. Others Liberase T-Flex Research Grade LSR Liberase T-Flex, Research Grade 3.5.3.3.11.8 0.2 μm filtered, lyophilizate 05989132001 Liberase T-Flex Research Grade Liberase T-Flex, Research Grade 07613336132081 Reagents, kits 1 kit 1 × 500 mg Collagenase blend2 × 15 mg Thermolysin CustomBiotech product. Please contact your local representative. Will be supplied as "Liberase T-Flex Research Grade". Unit of measure is "piece". EC 3.4.24.- en 9001-12-1 and 9073-78-3 en The Liberase T-Flex Research Grade kit contains highly purified collagenase class I and class II from Clostridium histolyticum. The two collagenase isoforms are blended in a precise ratio with each other. Highly purified thermolysin from Bacillus thermoproteolyticus is provided in a separate vial to allow individual adjustments of enzyme ratios for optimization of your tissue dissociation protocol. en How this product worksLiberase T-Flex Research Grade kit contains enzymes of purified collagenase isoforms I and II, and the neutral protease Thermolysin. The collagenase isoforms are purified from the fermentation of Clostridium histolyticum. Thermolysin is purified from the fermentation of Bacillus thermoproteolyticus. The methods for purification and blending of these components are described in one or more of the following patent numbers: US 5,753,485 and US 5,830,741. en The Liberase T-Flex Research Grade kit enables to make individual blends of collagenase and Thermolysin ratios. As a guideline please refer to the ratios described in the section “Target Activities” (Section General Considerations). Mix different ratio of neutral protease activity, relative to the collagenase activity to get an enzyme blend appropriate for your application. en The Liberase T-Flex, Research Grade kit enables individual blends of collagenase and thermolysin in low level ratios. The enzymes are manufactured for applications in research where high purity, consistent quality and low levels of bacterial endotoxins are required for reproducible high cell yield and viability. en I. Collagenase I/II MPBAppearance: White lyophilized cakeProtein mass/bottle (target: 537 mg protein): 430-644 mg proteinCollagenase I content(target: 322 mg): 258-386 mgCollagenase II content(target: 215 mg): 172-258 mgCollagenase Ib, c part of Collagenase I: ≤10 area%HPLC purity(total peak areas Collagenase I+II): ≥85 area%Activity (Wünsch), calculated: 2,172-3,617 U/bottleRatio Collagenase II/total Collagenase: 0.3-0.5Endotoxins: ≤10 EU/mg proteinBioburden: ≤10 CFU/bottleII. Thermolysin MPBAppearance: White lyophilized cakeProtein mass/bottle (target: 15.0 mg protein): 12.0-18.0 mg proteinActivity target neutral protease (calculated): 130,500-234,000 U/bottleHPLC purity (total peak area thermolysin): ≥85 area%Endotoxins: ≤50 EU/mg proteinBioburden: ≤10 CFU/bottle en