Others Liberase MTF C T GMP Grade Liberase MTF C/T, GMP Grade Blended Proteolytic Enzyme for Tissue Dissociation. For Further Processing only. 3.5.3.3.12.2 0.2 μm filtered, lyophilizate 05339880001 Liberase MTF C/T GMP Grade Liberase MTF C/T, GMP Grade 04038377026107 Reagents, kits 1 kit 2,000 U Collagenase I/II MTF, 2x15 mg Thermolysin MTF, 3x CustomBiotech product. Please contact your local representative. Will be supplied as "Liberase MTF C/T GMP Grade". Unit of measure is "piece". EC 3.4.24.- en 9001-12-1 and 9073-78-3 en Liberase MTF C/T (2:3) GMP grade is designed for in vitro enzymatic dissociation of tissue, enabling isolation of single cells.In case the product is intended to be used in clinical application the procedure must meet the applicable local laws and regulations including the involvement of ethic commissions if necessary.Verification of suitability of product for a specific application in all aspects is in the responsibility of the applicant. en Liberase MTF C/T GMP Grade kit is a highly purified enzyme blend for tissue dissociation where high purity, consistent quality and low levels of bacterial endotoxins are required in achieving reproducible high cell yield and viability. en Liberase MTF C/T, GMP Grade kit contains highly purified collagenase class I and class II from Clostridium histolyticum. The two collagenase isoforms are blended in a precise ratio with each other. Highly purified thermolysin from Bacillus thermoproteolyticus is provided in a separate vial to allow individual adjustments of enzyme ratios for optimization of your tissue dissociation protocol. en Maximum cell yield, viability, and functionalityUse of high-specific-activity blends with reduced clostripain, trypsin, and endotoxin content maximizes yield of viable, functional cells.Reduced contamination riskManufactured free of any mammalian- or avian-derived raw materials, avoiding the risk of transmission of animal diseases.High experimental reproducibilityHigh lot-to-lot consistency due to optimized enzyme fermentation and purification processes results in increased experimental reproducibility, ultimately saving time and resources.Flexible enzyme portfolioCustom sizes and blends are available upon request to better serve your unique application needs.Specific residual assayHighly sensitive assay developed specifically for detection of Liberase enzyme verifies removal from your final product. en I. Collagenase 2000 MTF MPBAppearance: White lyophilized cakeProtein/bottle (target: 537 mg protein): 430-644 mgCollagenase I content (target 322 mg protein): 258-386 mgCollagenase II content (target 215 mg protein): 172-258 mgCollagenase I b, c part of Collagenase I: ≤10 area%HPLC purity (total peak areas of Col I + Col II): ≥85%Activity (Wünsch, calculated): 2,172-3,617 U/vialRatio collagenase II/total collagenase (HPLC): 0.3-0.5Bioburden: ≤10 CFU/bottleEndotoxins: ≤10 EU/mg proteinII. Thermolysin MTF MPBAppearance: White lyophilized cakeProtein mass/bottle (target: 15.0 mg protein): 12.0-18.0 mgActivity neutral protease (calculated): 130,500-234,000 U/vialHPLC purity (total peak area thermolysin): ≥85%Bioburden: ≤10 CFU/bottleEndotoxins: ≤50 EU/mg protein en