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",
"Country": "XG",
"Code": "Content",
"Name": "Content"
},
{
"Language": "en",
"Value": "Liberase MTF C/T (2:3) GMP grade is designed for in vitro enzymatic dissociation of tissue, enabling isolation of single cells. In case the product is intended to be used in clinical application the procedure must meet the applicable local laws and regulations including the involvement of ethic commissions if necessary. Verification of suitability of product for a specific application in all aspects is in the responsibility of the applicant.",
"Country": "XG",
"Code": "Intended Use",
"Name": "Intended Use"
},
{
"Language": "en",
"Value": "
Dilute the reconstituted enzyme with an appropriate tissue dissociation buffer.
Use the tissue dissociation buffer immediately after reconstitution.
Determine the working concentration for each individual application.
The mass of protein in each vial may vary slightly from lot-to-lot according to the specific activities and assay variability for the enzyme constituents. For lot-specific details, refer to the corresponding Certificate of Analysis.
Do not add components that may influence enzyme activity, such as albumin, serum, or protease inhibitors.
",
"Country": "XG",
"Code": "Additional Equipment and Reagent Required",
"Name": "Additional Equipment and Reagent Required"
},
{
"Language": "en",
"Value": "The collagenases are produced by microbial fermentation. The fermentation media are free of any mammalian or avian derived raw materials. The downstream process is free of any animal derived raw materials, and no animal raw materials are added to the final preparation. Thermolysin is produced by microbial fermentation. The only animal derived raw materials used in the manufacturing process of the products are:
Fish gelatin derived from the skin of wild, deep-sea, cold water, kosher fish species.
Fish extract.
The downstream process is free of any animal derived raw materials, and no animal derived raw materials are added to the final preparation. Collagenase I/II MTF (2,000 U) enzyme blend GMP grade and Thermolysin MTF (15 mg) GMP grade are manufactured under GMP.
Laboratory procedures
Handle all samples as if potentially infectious, using safe laboratory procedures. As the sensitivity and titer of potential pathogens in the sample material varies, the operator must optimize pathogen inactivation by the Lysis / Binding Buffer or take appropriate measures, according to local safety regulations.
Do not eat, drink or smoke in the laboratory work area.
Do not pipette by mouth.
Wear protective disposable gloves, laboratory coats and eye protection, when handling samples and kit reagents.
Wash hands thoroughly after handling samples and reagents.
Waste handling
Discard unused reagents and waste in accordance with country, federal, state, and local regulations.
Safety Data Sheets (SDS) are available online on dialog.roche.com, or upon request from the local Roche office.
",
"Country": "XG",
"Code": "Safety Information",
"Name": "Safety Information"
},
{
"Language": "en",
"Value": "Liberase MTF C/T GMP Grade kit is a highly purified enzyme blend for tissue dissociation where high purity, consistent quality and low levels of bacterial endotoxins are required in achieving reproducible high cell yield and viability.",
"Country": "XG",
"Code": "Positioning",
"Name": "Positioning"
},
{
"Language": "en",
"Value": "Liberase MTF C/T, GMP Grade kit contains highly purified collagenase class I and class II from Clostridium histolyticum. The two collagenase isoforms are blended in a precise ratio with each other. Highly purified thermolysin from Bacillus thermoproteolyticus is provided in a separate vial to allow individual adjustments of enzyme ratios for optimization of your tissue dissociation protocol.",
"Country": "XG",
"Code": "Product Description",
"Name": "Product Description"
},
{
"Language": "en",
"Value": "
Maximum cell yield, viability, and functionality Use of high-specific-activity blends with reduced clostripain, trypsin, and endotoxin content maximizes yield of viable, functional cells.
Reduced contamination risk Manufactured free of any mammalian- or avian-derived raw materials, avoiding the risk of transmission of animal diseases.
High experimental reproducibility High lot-to-lot consistency due to optimized enzyme fermentation and purification processes results in increased experimental reproducibility, ultimately saving time and resources.
Flexible enzyme portfolio Custom sizes and blends are available upon request to better serve your unique application needs.
Specific residual assay Highly sensitive assay developed specifically for detection of Liberase enzyme verifies removal from your final product.
",
"Country": "XG",
"Code": "Benefits",
"Name": "Benefits"
},
{
"Language": "en",
"Value": "I. Collagenase 2000 MTF MPB Appearance: White lyophilized cake Protein/bottle (target: 537 mg protein): 430-644 mg Collagenase I content (target 322 mg protein): 258-386 mg Collagenase II content (target 215 mg protein): 172-258 mg Collagenase I b, c part of Collagenase I: ≤10 area% HPLC purity (total peak areas of Col I + Col II): ≥85% Activity (Wünsch, calculated): 2,172-3,617 U/vial Ratio collagenase II/total collagenase (HPLC): 0.3-0.5 Bioburden: ≤10 CFU/bottle Endotoxins: ≤10 EU/mg protein
