Others Liberase MNP-S GMP Grade Liberase MNP-S GMP Grade Blended Proteolytic Enzyme for Tissue Dissociation. For Further Processing only. 3.5.3.3.11.10 0.2 μm filtered, lyophilizate 06 297 790 001 6 297 790 001 06297790001 6297790001 06297790001 Liberase MNP-S 5mg GMP Liberase MNP-S GMP Grade 07613336132067 Reagents, kits 5 mg Not Available CustomBiotech product. Please contact your local representative. Will be supplied as "Liberase MNP-S GMP Grade". Unit of measure is "piece". EC 3.4.24.- en Liberase MNP-S GMP Grade is designed for in vitro enzymatic dissociation of segregated tissue, for the isolation of single cells.When the Liberase is intended for cellular and tissue-based product application, the procedure must meet the applicable local laws and regulations, including the involvement of ethic commissions if necessary.Verification of product use for a specific application is the sole responsibility of the applicant. en Liberase MNP-S is a highly purified enzyme blend for tissue dissociation where high purity, consistent quality, low levels of bacterial endotoxins and a sterile product are required for reproducible high cell yield and viability. en Liberase MNP-S contains highly purified collagenase class I and class II from Clostridium histolyticum. The two collagenase isoforms are blended in a precise ratio with each other and with a medium concentration of highly purified thermolysin, a neutral protease isolated from Bacillus thermoproteolyticus. The blend is manufactured in accordance with Good Manufacturing Practice (GMP). en Maximum cell yield, viability, and functionalityUse of high-specific-activity blends with reduced clostripain, trypsin, and endotoxin content maximizes yield of viable, functional cells.Reduced contamination riskManufactured free of any mammalian- or avian-derived raw materials, avoiding the risk of transmission of animal diseases.High experimental reproducibilityHigh lot-to-lot consistency due to optimized enzyme fermentation and purification processes results in increased experimental reproducibility, ultimately saving time and resources.Flexible enzyme portfolioCustom sizes and blends are available upon request to better serve your unique application needs.Specific residual assayHighly sensitive assay developed specifically for detection of Liberase enzyme verifies removal from your final product. en Appearance: White lyophilized cakeTotal protein (Mass/bottle): 5.5-8.3 mg (target: 6.9 mg protein)Collagenase I b, c part of collagenase I: ≤40 %areaCollagenase I content: 2.2-3.4 mg (target: 2.8 mg protein)Collagenase II content: 1.5-2.3 mg/bottle (target: 1.9 mg protein)Neutral Protease content: 1.8-2.6 mg (target 2.2 mg)Activity (Wünsch, calculated): 19-32 U/bottleActivity (Neutral Protease, calculated): 19,140 - 37,000 U/bottleRatio Collagenase II/Total Collagenase (HPLC): 0.3-0.5Endotoxin: ≤15 EU/mg protein en

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