{ "ProductData": { "RelatedLinks": "", "RegulatoryDisclaimer1": "", "RegulatoryDisclaimer2": "", "StainLocalization": [ "" ], "ProductType": "Others", "DisclaimerGroup2": null, "ProductNameGlobal": "Liberase MNP-S", "DisclaimerGroup1": null, "ControlTissue": [ "" ], "BrandName": "Liberase MNP-S", "LicenseDisclaimers": [ ], "Isotypes": "", "RegulatoryDisclaimer3": "Blended Proteolytic Enzyme for Tissue Dissociation. For Further Processing only.", "ID": "3.5.3.3.12.5", "ProductNameAddition": "0.2 μm filtered, lyophilizate", "Species": [ "" ], "Clone": "" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/Article_05578566001_im_en.png", "ImageType": "Image main" }, "Product2Taxomony": { "Product2TaxomonyReferences": [ { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "NodeName": "Liberase", "NodeID": "510" }, { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "NodeName": "Proteases", "NodeID": "70-044-00" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "Cell Isolation", "NodeID": "02-0134" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "Cell & Gene Therapy", "NodeID": "02-0133" }, { "StructureSystemIdentifier": "OWP_Organization", "StructureSystemName": "Organization", "NodeName": "CustomBiotech and LifeScience", "NodeID": "01-01-01-07-00" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "Enzymes", "NodeID": "02-0014" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "NodeName": "Tissue Dissociation", "NodeID": "330-00" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "NodeName": "CustomBiotech", "NodeID": "520" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeName": "Manufacturing", "NodeID": "180-00" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "Biologics", "NodeID": "02-0008" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "05578566001", "MaterialDescription": "Liberase MNP-S non GMP 35 mg", "RegisteredProductName": "Liberase MNP-S", "GTIN": "07613336132074", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "35 mg", "PackSizeDescPIM360": "", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"Liberase MNP-S\". Unit of measure is \"piece\"." } ] }, "Product2Products": { "Product2ProductReference": [ ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Language": "en", "Value": "Liberase MNP-S contains highly purified collagenase class I and class II from Clostridium histolyticum. The two collagenase isoforms are blended in a precise ratio with each other and with a medium concentration of highly purified thermolysin, a neutral protease isolated from Bacillus thermoproteolyticus.", "Name": "Product Description" }, { "Language": "en", "Value": "Will be supplied as \"Liberase MNP-S\". Unit of measure is \"piece\".", "Name": "CB - Order Information" }, { "Language": "en", "Value": "
VialLabelContentComposition
1Liberase MNP-SLyophilized enzyme blend Mammalian Tissue Free (MTF) Collagenase I/II and a medium content of Thermolysin MTF.1 vial,
35 mg
", "Name": "Content" }, { "Language": "en", "Value": "
For reconstitution of lyophilizate
  • Sterile, pyrogen-free water
For working solution
  • Tissue dissociation buffer
    Do not add components that may influence enzyme activity, such as albumin, serum, or protease inhibitors.
", "Name": "Additional Equipment and Reagent Required" }, { "Language": "en", "Value": "The collagenases are produced by microbial fermentation. The fermentation media are free of any mammalian or avian derived raw materials. The downstream process is free of any animal derived raw materials, and no animal raw materials are added to the final preparation.
Thermolysin is produced by microbial fermentation. The only animal derived raw materials used in the manufacturing process of the products are:
  • Fish gelatin derived from the skin of wild, deep-sea, cold water, kosher fish species.
  • Fish extract.
The downstream process is free of any animal derived raw materials, and no animal derived raw materials are added to the final preparation.
Laboratory procedures
  • Handle all samples as if potentially infectious, using safe laboratory procedures. As the sensitivity and titer of potential pathogens in the sample material varies, the operator must optimize pathogen inactivation by the Lysis / Binding Buffer or take appropriate measures, according to local safety regulations.
  • Do not eat, drink or smoke in the laboratory work area.
  • Do not pipette by mouth.
  • Wear protective disposable gloves, laboratory coats and eye protection, when handling samples and kit reagents.
  • Wash hands thoroughly after handling samples and reagents.
Waste handling
  • Discard unused reagents and waste in accordance with country, federal, state, and local regulations.
  • Safety Data Sheets (SDS) are available online on dialog.roche.com, or upon request from the local Roche office.
", "Name": "Safety Information" }, { "Language": "en", "Value": "
  1. Dilute the reconstituted enzyme with an appropriate tissue dissociation buffer.
    Use the tissue dissociation buffer immediately after reconstitution.
  2. Determine the working concentration for each individual application.
The mass of protein in each vial may vary slightly from lot-to-lot according to the specific activities and assay variability for the enzyme constituents. For lot-specific details, refer to the corresponding Certificate of Analysis.
", "Name": "Working Solution" }, { "Language": "en", "Value": "
  • Maximum cell yield, viability, and functionality
    Use of high-specific-activity blends with reduced clostripain, trypsin, and endotoxin content maximizes yield of viable, functional cells.
  • Reduced contamination risk
    Manufactured free of any mammalian- or avian-derived raw materials, avoiding the risk of transmission of animal diseases.
  • High experimental reproducibility
    High lot-to-lot consistency due to optimized enzyme fermentation and purification processes results in increased experimental reproducibility, ultimately saving time and resources.
  • Flexible enzyme portfolio
    Custom sizes and blends are available upon request to better serve your unique application needs.
  • Specific residual assay
    Highly sensitive assay developed specifically for detection of Liberase enzyme verifies removal from your final product.
", "Name": "Benefits" }, { "Language": "en", "Value": "Use Liberase MNP-S for in vitro enzymatic dissociation of tissue, to isolate single cells from a broad range of tissue types. In particular intended for use in isolation of tissue based stem cells and chondrocytes from cartilage.", "Name": "Applications" }, { "Language": "en", "Value": "Liberase MNP-S is a highly purified enzyme blend for tissue dissociation where high purity, consistent quality, low levels of bacterial endotoxins and a sterile product are required for reproducible high cell yield and viability.", "Name": "Positioning" }, { "Language": "en", "Value": "Appearance: White lyophilized cake
Total protein content: 41.5-62.3 mg/bottle (target: 51.9 mg protein)
Collagenase I b, c part of collagenase I: ≤40 area%
Collagenase I content: 16.8-25.2 mg/bottle (target: 21.0 mg protein)
Collagenase II content: 11.2-16.8 mg/bottle (target: 14.0 mg protein)
Thermolysin/neutral protease content: 13.5-20.3 mg/bottle (target: 16.9 mg protein)
Activity (Wünsch, calculated): 142-237 U/bottle
Activity (thermolysin/neutral protease, calculated): 147,030-275,000 U/bottle
Ratio collagenase II/total collagenase: 0.3-0.5
Endotoxin: ≤15 EU/mg protein", "Name": "Specification" }, { "Language": "en", "Value": "CAS Number: 9001-12-1 and 9073-78-3", "Name": "Properties" } ] } } ] }

Liberase MNP-S

0.2 μm filtered, lyophilizate

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