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After first use on the instrument, the reagents are stable for up to 12 weeks.


", "Name": "Storage Conditions (Working Solution)" }, { "Language": "en", "Value": "
Test protocol
Cedex Bio HT test definition
Test definitionLAC2B
(933)
LAC2D
(714)
Measuring range
     mmol/L
     mg/L
Standard
0.0444 – 15.55
4 – 1,400
High
0.444 – 155.5(1) 
40 – 14,000(1)
Control levelA 1, 2, 3A 2, 3
Sample predilutionNoNo
Measuring modeAbsorbanceAbsorbance
Abs. calc. modeEndpointEndpoint
Reaction modeR1-S-SRR1-S-SR
Reaction directionIncreaseIncrease
Wavelength A/B552/659 nm552/659 nm
Calc. first/last33/4933/49
Unitmmol/L, mg/Lmmol/L, mg/L
  1. Up to 1.55 mol/L (140 g/L) with automatic dilution.
Pipetting parameters
Pipetting parametersLAC2B
(933)
LAC2D
(714)
Water Diluent (Sample Predilution)NoNo
R1125 μL125 μL
Sample (S)2 μL + 20 μL water2 μL + 20 μL water
SR25 μL + 20 μL water25 μL + 20 μL water
Total Volume192 μL192 μL
Calibration
Calibration
CalibratorCalibrator A Bio (CAL A, 07-1100-4)
Calibration modeLinear regression
Calibration intervalEach new lot, and if recalibration is required due to QC results.
Quality control
Protocol LAC2B
  • Control A Level 1 Bio (CONA1, 07-1101-2)
  • Control A Level 2 Bio (CONA2, 07-1103-9)
  • Control A Level 3 Bio (CONA3, 07-1104-7)
Protocol LAC2D
  • Control A Level 2 Bio (CONA2, 07-1103-9)
  • Control A Level 3 Bio (CONA3, 07-1104-7)

Use the recommended control material. Other suitable control material can also be used.
Control intervals and acceptance limits should be adapted to each lab\u00adoratory's individual requirements. If values do not fall within the defined limits, corrective measures and recalibration are required.
Conversion factors
Conversion factors for lactate concentration:
  • 1 g/L = 11.10 mmol/L
  • 1 mmol/L = 90.09 mg/L
Traceability
This method has been standardized against a primary reference material.
Precision
Representative performance data on Cedex Bio HT Analyzers are shown. Results obtained in individual laboratories may differ. Precision was determined in samples of two concentration levels. Coefficients of variation (CV) were calculated for in-run precision (n=21) and inter-run precision (on 21 days).
 Level 1Level 2
Mean1.57 mmol/L (141 mg/L)3.07 mmol/L (277 mg/L)
CV in-run [%]0.70.8
CV inter-run [%]1.11.1
", "Name": "Protocols" }, { "Language": "en", "Value": "Kit for determination of lactate.", "Name": "Product Description" }, { "Language": "en", "Value": "Unit of measure is \"piece\".", "Name": "CB - Order Information" }, { "Language": "en", "Value": "
Analyzer and accessories
  • Cedex Bio HT Analyzer*, including accessories and disposables
  • Standard laboratory equipment
Calibrators and Controls
  • Calibrator A Bio*
  • Control A Level 1 Bio*
  • Control A Level 2 Bio*
  • Control A Level 3 Bio*
", "Name": "Additional Equipment and Reagent Required" }, { "Language": "en", "Value": "Lactate concentration can be determined in following range:
•LAC2B: 4 - 1400 mg/L (0.0444 - 15.55 mmol/L)
•LAC2D: 40 - 14000 mg/L (0.444 - 155.5 mmol/L),
up to 140 g/L (1.55 mol/L) with automatic dilution
Conversion Factors
Conversion factors for lactate concentration:
  • 1 g/L = 11.10 mmol/L
  • 1 mmol/L = 90.09 mg/L

Traceability
This method has been standardized against a primary reference material.

Precision
Representative performance data on Cedex Bio HT Analyzers are shown. Results obtained in individual laboratories may differ.
Precision was determined in samples of two concentration levels. Coefficients of variation (CV) were calculated for in-run precision (n=21) and inter-run precision (on 21 days).

 Level 1Level 2
Mean1.57 mmol/L (141 mg/L)3.07 mmol/L (277 mg/L)
CV in-run0.7%0.8%
CV inter-run1.1%1.1%
", "Name": "Background Information - Help Corner" }, { "Language": "en", "Value": "
VialContentComposition
LAC2BReagent cassette,
100 tests
Reagent 1 (R1): Hydrogen donor, 1.75 mmol/L; ascorbate oxidase (cucumber), 501 μkat/L; buffer; preservative.
Start Reagent (SR): 4-aminoantipyrine, 5 mmol/L; lactate oxidase (microbial), 251 μkat/L; peroxidase (horseradish), 401 μkat/L; buffer; preservative.
", "Name": "Content" }, { "Language": "en", "Value": "Lactate is an important metabolyte in cell cultures, and accumulation of lactate can suppress cell growth and product formation. For monitoring in a culture process, lactate can be determined on a Cedex Bio or Cedex Bio HT Analyzer with high reliability and convenience.", "Name": "Applications" }, { "Language": "en", "Value": "Method: Enzymatic photometric assay using lactate oxidase.
Recovery: ±10%", "Name": "Specification" } ] } } ] }

Lactate Bio HT

Test kit for the Cedex Bio HT Analyzer

Cedex Lactate

Overview

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Technical documents

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