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Test protocol
Cedex Bio HT test definition
Test definitionLAC2B
(933)
LAC2D
(714)
Measuring range
     mmol/L
     mg/L
Standard
0.0444 – 15.55
4 – 1,400
High
0.444 – 155.5(1) 
40 – 14,000(1)
Control levelA 1, 2, 3A 2, 3
Sample predilutionNo1:10
Measuring modeAbsorbanceAbsorbance
Abs. calc. modeEndpointEndpoint
Reaction modeR1-S-SRR1-S-SR
Reaction directionIncreaseIncrease
Wavelength A/B552/659 nm552/659 nm
Calc. first/last33/4933/49
Unitmmol/L, mg/Lmmol/L, mg/L
  1. Up to 1.55 mol/L (140 g/L) with automatic dilution.
Pipetting parameters
Pipetting parametersLAC2B
(933)
LAC2D
(714)
Water Diluent (Sample Predilution)(1)No20 μL sample + 180 μL water
R1125 μL125 μL
Sample (S)2 μL + 20 μL water2 μL + 20 μL water
SR25 μL + 20 μL water25 μL + 20 μL water
Total Volume192 μL192 μL
  1. Sample predilution is performed in a prior step, after which the reaction mix is prepared in the same way as the unmodified sample. 
Calibration
Calibration
CalibratorCalibrator A Bio (CAL A, 07-1100-4)
Calibration modeLinear regression
Calibration intervalEach new lot, and if recalibration is required due to QC results.
Quality control
Protocol LAC2B
  • Control A Level 1 Bio (CONA1, 07-1101-2)
  • Control A Level 2 Bio (CONA2, 07-1103-9)
  • Control A Level 3 Bio (CONA3, 07-1104-7)
Protocol LAC2D
  • Control A Level 2 Bio (CONA2, 07-1103-9)
  • Control A Level 3 Bio (CONA3, 07-1104-7)

Use the recommended control material. Other suitable control material can also be used.
Control intervals and acceptance limits should be adapted to each lab\u00adoratory's individual requirements. If values do not fall within the defined limits, corrective measures and recalibration are required.
Conversion factors
Conversion factors for lactate concentration:
  • 1 g/L = 11.10 mmol/L
  • 1 mmol/L = 90.09 mg/L
Traceability
This method has been standardized against a primary reference material.
Precision
Representative performance data on Cedex Bio HT Analyzers are shown. Results obtained in individual laboratories may differ. Precision was determined in samples of two concentration levels. Coefficients of variation (CV) were calculated for in-run precision (n=21) and inter-run precision (on 21 days).
 Level 1Level 2
Mean1.57 mmol/L (141 mg/L)3.07 mmol/L (277 mg/L)
CV in-run [%]0.70.8
CV inter-run [%]1.11.1
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Analyzer and accessories
  • Cedex Bio HT Analyzer*, including accessories and disposables
  • Standard laboratory equipment
Calibrators and Controls
  • Calibrator A Bio*
  • Control A Level 1 Bio*
  • Control A Level 2 Bio*
  • Control A Level 3 Bio*
", "Country": "XG", "Code": "Additional Equipment and Reagent Required", "Name": "Additional Equipment and Reagent Required" }, { "Language": "en", "Value": "
VialContentComposition
LAC2BReagent cassette,
100 tests
Reagent 1 (R1): Hydrogen donor, 1.75 mmol/L; ascorbate oxidase (cucumber), 501 μkat/L; buffer; preservative.
Start Reagent (SR): 4-aminoantipyrine, 5 mmol/L; lactate oxidase (microbial), 251 μkat/L; peroxidase (horseradish), 401 μkat/L; buffer; preservative.
", "Country": "XG", "Code": "Content", "Name": "Content" }, { "Language": "en", "Value": "Lactate is an important metabolyte in cell cultures, and accumulation of lactate can suppress cell growth and product formation. For monitoring in a culture process, lactate can be determined on a Cedex Bio or Cedex Bio HT Analyzer with high reliability and convenience.", "Country": "XG", "Code": "Applications", "Name": "Applications" }, { "Language": "en", "Value": "Method: Enzymatic photometric assay using lactate oxidase.
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Lactate Bio HT

Test kit for the Cedex Bio HT Analyzer

Cedex Lactate

Ordering Information

Technical documents

for certificates, method sheets (instructions for use), safety data sheets and software downloads
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