{ "ProductData": { "ID": "3.1.1.4.13.5", "ProductType": "Others", "BrandName": "L-Lactate Dehydrogenase (L-LDH)", "ProductNameAddition": "from pig muscle, for use of AST/GOT-Determination according to IFCC recommendations, lyophilizate", "RegulatoryDisclaimer1": "For further processing only.", "DisclaimerGroup1": "", "RegulatoryDisclaimer2": null, "DisclaimerGroup2": null, "RegulatoryDisclaimer3": null, "SampleType": [ ], "LicenseDisclaimers": [ ], "RelatedLinks": "", "Clone": "", "ControlTissue": [ "" ], "ISOtypes": "", "Species": [ "" ], "StainLocalization": [ "" ], "ProductNameGlobal": "L-Lactate Dehydrogenase L-LDH lyophilizate" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/CB_IMAGE.png", "ImageType": "Image main" }, "Product2Taxonomy": { "Product2TaxonomyReferences": [ { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "StructureNodeStatus": "valid", "NodeName": "Enzymes", "NodeID": "02-0014" }, { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "StructureNodeStatus": "valid", "NodeName": "Dehydrogenases", "NodeID": "70-014-00" }, { "StructureSystemIdentifier": "OWP_Organization", "StructureSystemName": "Organization", "StructureNodeStatus": "valid", "NodeName": "CustomBiotech and LifeScience", "NodeID": "01-01-01-07-00" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "StructureNodeStatus": "valid", "NodeName": "Clinical Chemistry Raw Materials", "NodeID": "02-0011" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "StructureNodeStatus": "valid", "NodeName": "Enzyme Testing", "NodeID": "06-00-00" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "StructureNodeStatus": "valid", "NodeName": "CustomBiotech", "NodeID": "520" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "180-00" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "999-00" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "999" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "99-00-00" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "10254754103", "MaterialDescription": "LDH IFCC-quality from Hog Muscle", "RegisteredProductName": "L-Lactate Dehydrogenase (L-LDH)", "GTIN": "", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "custom fill", "PackSizeDescPIM360": "Not Available", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"LDH IFCC-quality from Hog Muscle\". Unit of measure is \"MU\".
Additional formulation: Suspension in glycerol solution, Catalog No. 10 417 718 103" } ] }, "Product2Products": { "Product2ProductReference": [ { "ProductID": "3.1.1.4.13.2", "BrandName": "L-Lactate Dehydrogenase (L-LDH)", "ProductNameAddition": "from pig muscle, for use of AST/GOT-Determination according to IFCC recommendations, solution", "ReferenceType": "Reagent", "Classification": [ { "IdentifierofStructureSystem": "Product_Grouping", "NameofStructureSystem": "Product Grouping", "StructureNodeID": "02-0014", "StructureGroupPath": "CustomBiotech->Enzymes", "StructureGroupName": "Enzymes", "StructureNodeStatus": "valid" }, { "IdentifierofStructureSystem": "OWP_Product_Types", "NameofStructureSystem": "Product Types", "StructureNodeID": "70-014-00", "StructureGroupPath": "Raw Materials->Dehydrogenases", "StructureGroupName": "Dehydrogenases", "StructureNodeStatus": "valid" }, { "IdentifierofStructureSystem": "OWP_Organization", "NameofStructureSystem": "Organization", "StructureNodeID": "01-01-01-07-00", "StructureGroupPath": "Roche Diagnostics Solution (RDS)->Customer Areas->Core lab->CustomBiotech and LifeScience", "StructureGroupName": "CustomBiotech and LifeScience", "StructureNodeStatus": "valid" }, { "IdentifierofStructureSystem": "Product_Grouping", "NameofStructureSystem": "Product Grouping", "StructureNodeID": "02-0011", "StructureGroupPath": "CustomBiotech->Clinical Chemistry Raw Materials", "StructureGroupName": "Clinical Chemistry Raw Materials", "StructureNodeStatus": "valid" }, { "IdentifierofStructureSystem": "Applications", "NameofStructureSystem": "Applications", "StructureNodeID": "06-00-00", "StructureGroupPath": "Enzyme Testing", "StructureGroupName": "Enzyme Testing", "StructureNodeStatus": "valid" }, { "IdentifierofStructureSystem": "Product_Solutions", "NameofStructureSystem": "Product Solutions", "StructureNodeID": "520", "StructureGroupPath": "CustomBiotech", "StructureGroupName": "CustomBiotech", "StructureNodeStatus": "valid" }, { "IdentifierofStructureSystem": "Lab_Type", "NameofStructureSystem": "Lab Types", "StructureNodeID": "180-00", "StructureGroupPath": "", "StructureGroupName": "", "StructureNodeStatus": "invalid" }, { "IdentifierofStructureSystem": "OWP_Techniques", "NameofStructureSystem": "Techniques", "StructureNodeID": "999-00", "StructureGroupPath": "", "StructureGroupName": "", "StructureNodeStatus": "invalid" }, { "IdentifierofStructureSystem": "Disease_Areas", "NameofStructureSystem": "Disease Areas", "StructureNodeID": "99-00-00", "StructureGroupPath": "", "StructureGroupName": "", "StructureNodeStatus": "invalid" }, { "IdentifierofStructureSystem": "Pathogens", "NameofStructureSystem": "Pathogens", "StructureNodeID": "99-00-00", "StructureGroupPath": "", "StructureGroupName": "", "StructureNodeStatus": "invalid" }, { "IdentifierofStructureSystem": "OWP_Family", "NameofStructureSystem": "Product Families", "StructureNodeID": "999", "StructureGroupPath": "", "StructureGroupName": "", "StructureNodeStatus": "invalid" }, { "IdentifierofStructureSystem": "Health_Topics", "NameofStructureSystem": "Health Topics", "StructureNodeID": "99-00-00", "StructureGroupPath": "", "StructureGroupName": "", "StructureNodeStatus": "invalid" } ] } ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Language": "en", "Value": "Will be supplied as \"LDH IFCC-quality from Hog Muscle\". Unit of measure is \"MU\".
Additional formulation: Suspension in glycerol solution, Catalog No. 10 417 718 103", "Country": "XG", "Code": "CB - Order Information", "Name": "CB - Order Information" }, { "Language": "en", "Value": "Use L-Lactate Dehydrogenase in a variety of diagnostic tests for the removal of pyruvate in determinations working with NADH (i.e., triglycerides, lipase, aldolase, aminotransferases, glutamate dehydrogenase).", "Country": "XG", "Code": "Applications", "Name": "Applications" }, { "Language": "en", "Value": "Dehydrogenase that catalyzes the interconversion of specific for L(+)-lactate to pyruvate.", "Country": "XG", "Code": "Positioning", "Name": "Positioning" }, { "Language": "en", "Value": "Nomenclature: L-lactate:NAD+ oxidoreductase
CAS Number: 9001-60-9
Molecular weight: 140 kD
Isoelectric point: 4.6
Michaelis constants (Phosphate buffer, pH 7.5; +25°C):
Pyruvate: 1.5 x 10-4 mol/L (NADH: 0.18 mmol/L)
L-lactate: 3.3 x 10-3 mol/L (NAD: 0.5 mmol/L)
NADH: 1.1 x 10-5 mol/L (Pyruvate: 0.6 mmol/L)
NAD: 6.7 x 10-5 mol/L (L-lactate: 34 mmol/L)
Inhibitors: Oxamate, pyruvate (excess), oxalate, Ag+, Hg2+, Cu2+
pH optimum: 3.0-7.0 (see figure)
Temperature dependence: See figure
pH stability: 5.5-8.5 (see figure)
Thermal stability: Up to +40°C (see figure)
Specificity: Lactate dehydrogenase is specific for L(+)-lactate, D(-)-lactate does not react. Glyoxylate is also a lactate dehydrogenase substrate. Apart from pyruvate some 2-oxoacids are reduced. NAD analogs (e.g., APAD) react at similar rates.", "Country": "XG", "Code": "Properties", "Name": "Properties" }, { "Language": "en", "Value": "Appearance: White lyophilizate
Solubility: Clear colorless solution in water (c=10 mg/mL)
pH value (c=10 mg/mL in water): 6.0-7.0
Activity (+30°C, pyruvate; according to IFCC recommendations):
for Aspartate aminotransferase (AST/GOT) determination: ≥50 U/mg lyophilizate
for Alanine aminotransferase (ALT/GPT) determination: ≥50 U/mg lyophilizate
Activity (mean value of both determinations): ≥50 U/mg lyophilizate
Contaminants (expressed as percentage of Lactate Dehydrogenase activity, assayed according to the IFCC recommendations):
Aspartate aminotransferase (AST/GOT): ≤0.001
unspecificity of Lactate dehydrogenase: ≤0.005
Alanine aminotransferase (ALT/GPT): ≤0.001
unspecificity of Lacatae dehydrogenase: ≤0.005
SVD free: Corresponds to specification
pH 5.5 treatment (30 minutes): Corresponds to specification
Stability: At +2 to +8°C within specification range for 12 months.", "Country": "XG", "Code": "Specification", "Name": "Specification" } ] } } ] }

L-Lactate Dehydrogenase (L-LDH)

from pig muscle, for use of AST/GOT-Determination according to IFCC recommendations, lyophilizate

logo

Ordering Information

Technical documents

for certificates, method sheets (instructions for use), safety data sheets and software downloads
error errorMessage
Sorry, we couldn't find the content you are looking for
Please try again later