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"Value": "2x concentrated, ready-to-use antibody-mediated hot start PCR mix, containing KAPA2G DNA Polymerase in an optimized concentration for amplification of crude sample types and/or AT- or GC-rich targets.",
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KAPA2G Robust HotStart ReadyMix (2X)2 x 6.25 ml bottle, contains 2 mM MgCl
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- Simplify your workflow by working with crude samples.KAPA2G Robust shows high tolerance to inhibitor carry-over and crude sample PCR (e.g. FFPE)
- Use the same protocol for difficult targets.
Work with GC- and AT-rich targets and shorten optimization time of your assays
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"Value": "Amplification of DNA fragments up to 3 kb in PCR assays from a wide variety of templates. Particularly suited for:
- Assays which perform poorly with wild-type Taq
- Amplification of DNA fragments with high GC- or AT-content
- Amplification from template samples that contain PCR inhibitors (e.g. salts, urea, SDS, ethanol, EDTA) at concentrations that inhibit wild-type Taq
- Amplification from crude samples, e.g. colony PCR, or PCR from crude extracts, such as those prepared using KAPA Express Extract.
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Unspecific endonucleases (plasmid DNA): Not detectable after 8 hours incubation at 37°C.
Exonucleases (λ DNA): Not detectable after 8 hours incubation at 37°C.
Tests for the presence of contaminating nucleic acids (
E. coli and related strains genomic DNA, 411 bp 16S rRNA fragment, <50 fg/μL): Corresponds to specification
(human genomic DNA, 290 bp b-actin fragment, <0.5 pg/μL): Corresponds to specification
Performance test (≥ 0.1 ng human genomic DNA): Corresponds to specification",
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