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Handle all samples as if potentially infectious, using safe laboratory procedures. As the sensitivity and titer of potential pathogens in the sample material can vary, the operator must optimize pathogen inactivation and follow the appropriate measures according to local safety regulations.
Do not eat, drink, or smoke in the laboratory work area.
Do not pipette by mouth.
Wear protective disposable gloves, laboratory coats, and eye pro\u00adtection, when handling samples and kit reagents.
Wash hands thoroughly after handling samples and reagents.
Waste handling
Discard unused reagents and waste in accordance with country, federal, state, and local regulations.
Safety Data Sheets (SDS) are available online at dialog.roche.com, or upon request from the local Roche office.
",
"Language": "en",
"Country": "XG",
"Code": "Content"
},
{
"Name": "Applications",
"Value": "Iron is an essential trace metal required in cell cultures and microbial fermentation. For optimal cell growth and product formation the iron concentration has to be kept whithin an appropriate concentration range, providing sufficient nutrition and avoiding toxical inhibition. For control of culture media and for monitoring in a culture process, the iron concentration can be determined on a Cedex Bio or Cedex Bio HT Analyzer with high reliability and convenience.",
"Language": "en",
"Country": "XG",
"Code": "Applications"
},
{
"Name": "Specification",
"Value": "Method: Colorimetric detection with FerroZine. Recovery: ±10%",
"Language": "en",
"Country": "XG",
"Code": "Specification"
},
{
"Name": "Background Information - Help Corner",
"Value": "Due to its test design, the assay detects both Fe2+ and Fe3+ irons
The Iron assay detects all solved iron, independently of the oxidation state (Fe2+ or Fe3+). This is a requirement for the assay, because Fe2+ easily converts to Fe3+ in presence of oxygen and backwards. In a first step of the assay, Fe ions are released from complexes and Fe3+ is reduced to Fe2+. Then the total Fe2+ reacts with the chromogenic reagent for detection:
Complexed iron is liberated at pH<2, resulting in free iron ions.
Fe3+ ions are reduced by ascorbate to Fe2+
Fe2+ ions and FerroZine form a colored complex
The absorbance measured at 552nm is directly proportional to the iron concentration of the sample (Fe2+ and Fe3+ irons)
",
"Language": "en",
"Country": "XG",
"Code": "Background Information - Help Corner"
}
]
}
}
]
}