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"Code": "Storage Conditions (Product)" }, { "Name": "CB - Order Information", "Value": "Unit of measure is \"piece\".", "Language": "en", "Country": "XG", "Code": "CB - Order Information" }, { "Name": "Additional Equipment and Reagent Required", "Value": "

• Cedex Bio HT Analyzer*, including accessories and disposables
• Standard laboratory equipment

• Calibrator C Bio*
• Control C Level 1 Bio*
• Control C Level 2 Bio*
• Control C Level 3 Bio*

", "Language": "en", "Country": "XG", "Code": "Additional Equipment and Reagent Required" }, { "Name": "Safety Information", "Value": "Precautions

• Handle all samples as if potentially infectious, using safe laboratory procedures. As the sensitivity and titer of potential pathogens in the sample material can vary, the operator must optimize pathogen inactivation and follow the appropriate measures according to local safety regulations.
• Do not eat, drink, or smoke in the laboratory work area.
• Do not pipette by mouth.
• Wear protective disposable gloves, laboratory coats, and eye pro\u00adtection, when handling samples and kit reagents.
• Wash hands thoroughly after handling samples and reagents.

Waste handling

• Discard unused reagents and waste in accordance with country, federal, state, and local regulations.
• Safety Data Sheets (SDS) are available online at dialog.roche.com, or upon request from the local Roche office.

", "Language": "en", "Country": "XG", "Code": "Safety Information" }, { "Name": "Content", "Value": "

Vial	Content	Composition
FE2B	Reagent cassette, 200 tests	Reagent 1 (R1): Citric acid, 200 mmol/L, thiourea, 115 mmol/L; non-reactive surfactant.
Start Reagent (SR): Sodium ascorbate, 150 mmol/L; FerroZine, 6 mmol/L; preservative.

", "Language": "en", "Country": "XG", "Code": "Content" }, { "Name": "Applications", "Value": "Iron is an essential trace metal required in cell cultures and microbial fermentation. For optimal cell growth and product formation the iron concentration has to be kept whithin an appropriate concentration range, providing sufficient nutrition and avoiding toxical inhibition. For control of culture media and for monitoring in a culture process, the iron concentration can be determined on a Cedex Bio or Cedex Bio HT Analyzer with high reliability and convenience.", "Language": "en", "Country": "XG", "Code": "Applications" }, { "Name": "Specification", "Value": "Method: Colorimetric detection with FerroZine.
Recovery: ±10%", "Language": "en", "Country": "XG", "Code": "Specification" }, { "Name": "Background Information - Help Corner", "Value": "Due to its test design, the assay detects both Fe²⁺ and Fe³⁺ irons

The Iron assay detects all solved iron, independently of the oxidation state (Fe²⁺ or Fe³⁺). This is a requirement for the assay, because Fe²⁺ easily converts to Fe³⁺ in presence of oxygen and backwards. In a first step of the assay, Fe ions are released from complexes and Fe³⁺ is reduced to Fe²⁺. Then the total Fe²⁺ reacts with the chromogenic reagent for detection:

• Complexed iron is liberated at pH<2, resulting in free iron ions.
• Fe³⁺ ions are reduced by ascorbate to Fe²⁺
• Fe²⁺ ions and FerroZine form a colored complex
• The absorbance measured at 552nm is directly proportional to the iron concentration of the sample (Fe²⁺ and Fe³⁺ irons)

", "Language": "en", "Country": "XG", "Code": "Background Information - Help Corner" } ] } } ] }