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"Health_Topics", "NameofStructureSystem": "Health Topics", "StructureNodeID": "99-00-00", "StructureGroupPath": "NOT APPLICABLE", "StructureGroupName": "NOT APPLICABLE" } ] } ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Language": "en", "Value": "Keep at +2 to +10°C for on-board use.
\nOn-board stability: After first use on the Analyzer, the reagents are stable for up to 4 weeks.", "Name": "Storage Conditions (Working Solution)" }, { "Language": "en", "Value": "Store the reagent at +2 to +8°C.", "Name": "Storage Conditions (Product)" }, { "Language": "en", "Value": "Unit of measure is \"piece\".", "Name": "CB - Order Information" }, { "Language": "en", "Value": "This product is intended for quantitative determination of iron ions in aqueous solutions using the Cedex Bio Analyzer.", "Name": "Product Description" }, { "Language": "en", "Value": "Iron is an essential trace metal required in cell cultures and microbial fermentation. For optimal cell growth and product formation the iron concentration has to be kept whithin an appropriate concentration range, providing sufficient nutrition and avoiding toxical inhibition. For control of culture media and for monitoring in a culture process, the iron concentration can be determined on a Cedex Bio or Cedex Bio HT Analyzer with high reliability and convenience.", "Name": "Applications" }, { "Language": "en", "Value": "
VialCapContentComposition
1 FE2B R1white2 vials,
50 tests each
Reagent 1 (R1): Citric acid, 200 mmol/L, thiourea, 115 mmol/L; non-reactive surfactant.
2 FE2B SRblack2 vials,
50 tests each
Start Reagent (SR): Sodium ascorbate, 150 mmol/L; FerroZine, 6 mmol/L; preservative.
", "Name": "Content" }, { "Language": "en", "Value": "Applications FE2B (929), FE2D (924)
\nFE2B is the standard protocol for iron determination. If the iron concentration of samples is higher than 10 mg/L (179 μmol/L), then protocol FE2D should be used (same protocol with a preset 1:10 sample predilution).
\nResults higher than the valid measuring range will be flagged and a rerun with an automatic 1:10 predilution will be recommended by the software.
\n
\nCedex Bio test definition

\nMeasuring mode               Absorbance
\nAbs. calculation mode      Endpoint
\nReaction direction             Increase
\nWavelength A/B                552/659 nm
\nCalc. first/last                    26/30
\nUnit                                    μmol/L
\nReaction mode                  R1-S-SR
\n
\nPipetting parameters
\nR1                                     100 μL
\nSample                              8.5 μL + 11.5 μL Diluent (H2O)
\nSR                                       20 μL + 20 μL Diluent (H2O)
\nTotal volume                     160 μL
\n
\nCalibration
\nCalibrator                          Calibrator C Bio (CAL C, 484)
\nCalibration mode               Linear regression
\nCalibration intervall           Each new lot, and if recalibration is required due to QC results
\n
\nQuality controls
\nControl C Level 1 Bio (CONC1, 501)
\nControl C Level 2 Bio (CONC2, 502)
\nControl C Level 3 Bio (CONC3, 503)
\n
\nUse the recommended control material. Other suitable control material can be used in addition.
\nControl intervals and acceptance limits should be adapted to each laboratory's individual requirements.
\nIf values do not fall into the defined limits, corrective measures and recalibration are required", "Name": "Protocols - Help Corner" }, { "Language": "en", "Value": "Due to its test design, the assay detects both Fe2+ and Fe3+ irons:

The Iron assay detects all solved iron, independently of the oxidation state (Fe2+ or Fe3+). This is a requirement for the assay, because Fe2+ easily converts to Fe3+ in presence of oxygen and backwards. In a first step of the assay, Fe ions are released from complexes and Fe3+ is reduced to Fe2+. Then the total Fe2+ reacts with the chromogenic reagent for detection:
  • Complexed iron is liberated at pH<2, resulting in free iron ions.
  • Fe3+ ions are reduced by ascorbate to Fe2+
  • Fe2+ ions and FerroZine form a colored complex
  • The absorbance measured at 552nm is directly proportional to the iron concentration of the sample (Fe2+ and Fe3+ irons)
", "Name": "Background Information - Help Corner" }, { "Language": "en", "Value": "
Analyzer and accessories
  • Cedex Bio Analyzer*, including accessories and disposables
  • Standard laboratory equipment
Calibrators and Controls
  • Calibrator C Bio*
  • Control C Level 1 Bio*
  • Control C Level 2 Bio*
  • Control C Level 3 Bio*
", "Name": "Additional Equipment and Reagent Required" }, { "Language": "en", "Value": "Method: Colorimetric detection with FerroZine.
Recovery: ±10%", "Name": "Specification" }, { "Language": "en", "Value": "Precautions
  • Handle all samples as if potentially infectious, using safe laboratory procedures. As the sensitivity and titer of potential pathogens in the sample material can vary, the operator must optimize pathogen inactivation and follow the appropriate measures according to local safety regulations.
  • Do not eat, drink, or smoke in the laboratory work area.
  • Do not pipette by mouth.
  • Wear protective disposable gloves, laboratory coats, and eye pro\u00adtection, when handling samples and kit reagents.
  • Wash hands thoroughly after handling samples and reagents.
Waste handling
  • Discard unused reagents and waste in accordance with country, federal, state, and local regulations.
  • Safety Data Sheets (SDS) are available online at dialog.roche.com, or upon request from the local Roche office.
", "Name": "Safety Information" } ] } } ] }

Iron Bio

Test kit for the Cedex Bio Analyzer

Cedex Iron

Overview

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Technical documents

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