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"Language": "en",
"Value": "Will be supplied as \"Hexokinase (HK) from Recombinant Yeast\". Unit of measure is \"MU\".",
"Country": "XG",
"Code": "CB - Order Information",
"Name": "CB - Order Information"
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"Value": "Use Hexokinase in diagnostic tests for blood glucose using the hexokinase method and for the determination of creatine kinase.",
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"Value": "Recombinant enzyme that converts hexose to hexose-6-phosphate.",
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Nomenclature: ATP:D-hexose 6-phosphotransferase
CAS Number: 9001-51-8
Molecular weight: 57 kD (SDS-PAGE)
Isoelectric point: 4.5-5.0
Michaelis constants (D-glucose)
:Phosphate buffer, 0.1 mol/L, pH 7.0; +25°C: 3.05 x 10
-4 mol/L
Phosphate buffer, 0.1 mol/L, pH 7.4; +30°C: 1.90 x 10
-4 mol/L
Tea buffer, 0.1 mol/L, pH 7.6; +25°C: 2.30 x 10
-4 mol/L
Michaelis constants (ATP):
Tris buffer, 0.1 mol/L, pH 7.6; +28°C: 1.60 x 10
-4 mol/L
Tea buffer, 0.1 mol/L, pH 7.6; +25°C: 1.90 x 10
-4 mol/L
Inhibitors: EDTA, SH-blocking compounds, sorbose-1-phosphate, polyphosphates, 6-deoxy-6-fluoroglucose, 2-C-hydroxymethylglucose, lyxose.
Activators: Mg
2+, catecholamines
pH optimum: 7.0-10.0 (see figure)
Temperature dependence: See figure
pH stability: 5.0-9.0 (see figure)
Thermal stability: 100% for 20 minutes at +37°C, 50% for 20 minutes at +45°C, 75% for 5 hours at +37°C (see figures)
Specificity: Hexokinase phosphorylates D-glucose, D-fructose, D-mannose, D-glucosamin, 2-deoxyglucose. L-Arabinose, D-xylose, L-rhamnose, D-galactose, D-lactose, sucrose, maltose, trehalose, raffinose, N-acetyl glucosamine do not react. ATP can be partially replaced by other nucleotides.",
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Appearance: Clear, yellowish solution, in 50% glycerol (v/v)
pH value: 6.0-7.0
Activity (+25°C, glucose): ≥1200 U/mL
Activity (+30°C): ≥1680 U/mL
Protein (Biuret): ≥75 mg/mL
Contaminants (expressed as percentage of Hexokinase activity):
Alcohol dehydrogenase: ≤0.001
ATPase: ≤0.05
Creatine kinase: ≤0.001
G6P-DH: ≤0.005
Glutamate dehydrogenase: ≤0.01
Glutathione reductase: ≤0.005
Myokinase: ≤0.001
6-Phosphogluconate dehydrogenase: ≤0.001
Phophoglucose isomerase: ≤0.002
Phosphoglucomutase: ≤0.02
Glucose: ≤0.125 μg/mg lyophilizate
Glycerol (enzymatic): 560-680 mg/mL ≙ 45-55%
Stability: At +2 to +8°C within specification range for 18 months.",
"Country": "XG",
"Code": "Specification",
"Name": "Specification"
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