{ "ProductData": { "RelatedLinks": "", "RegulatoryDisclaimer1": "For further processing only.", "RegulatoryDisclaimer2": "", "StainLocalization": [ "" ], "ProductType": "Others", "DisclaimerGroup2": null, "ProductNameGlobal": "Hexokinase HK solution", "DisclaimerGroup1": "Non-IVD", "ControlTissue": [ "" ], "BrandName": "Hexokinase (HK)", "LicenseDisclaimers": [ ], "Isotypes": "", "RegulatoryDisclaimer3": "", "ID": "3.1.1.3.9.6", "ProductNameAddition": "from yeast overproducer, solution", "Species": [ "" ], "Clone": "" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/CB_IMAGE.png", "ImageType": "Image main" }, "Product2Taxomony": { "Product2TaxomonyReferences": [ { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "Enzymes", "NodeID": "02-0014" }, { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "NodeName": "Kinases", "NodeID": "70-029-00" }, { "StructureSystemIdentifier": "OWP_Organization", "StructureSystemName": "Organization", "NodeName": "CustomBiotech and LifeScience", "NodeID": "01-01-01-07-00" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "Clinical Chemistry Raw Materials", "NodeID": "02-0011" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeName": "Carbohydrate Testing", "NodeID": "01-00-00" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeName": "Cardiac Testing", "NodeID": "02-00-00" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeName": "Enzyme Testing", "NodeID": "06-00-00" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "NodeName": "CustomBiotech", "NodeID": "520" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeName": "Manufacturing", "NodeID": "180-00" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "NodeName": "NOT APPLICABLE", "NodeID": "999-00" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "NodeName": "NOT APPLICABLE", "NodeID": "999" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "11149130103", "MaterialDescription": "Hexokinase (HK) from Recombinant Yeast", "RegisteredProductName": "Hexokinase (HK)", "GTIN": "", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "custom fill", "PackSizeDescPIM360": "", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"Hexokinase (HK) from Recombinant Yeast\". Unit of measure is \"MU\"." } ] }, "Product2Products": { "Product2ProductReference": [ ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Language": "en", "Value": "Will be supplied as \"Hexokinase (HK) from Recombinant Yeast\". Unit of measure is \"MU\".", "Name": "CB - Order Information" }, { "Language": "en", "Value": "Use Hexokinase in diagnostic tests for blood glucose using the hexokinase method and for the determination of creatine kinase.", "Name": "Applications" }, { "Language": "en", "Value": "Recombinant enzyme that converts hexose to hexose-6-phosphate.", "Name": "Positioning" }, { "Language": "en", "Value": "Nomenclature: ATP:D-hexose 6-phosphotransferase
CAS Number: 9001-51-8
Molecular weight: 57 kD (SDS-PAGE)
Isoelectric point: 4.5-5.0
Michaelis constants (D-glucose):
Phosphate buffer, 0.1 mol/L, pH 7.0; +25°C: 3.05 x 10-4 mol/L
Phosphate buffer, 0.1 mol/L, pH 7.4; +30°C: 1.90 x 10-4 mol/L
Tea buffer, 0.1 mol/L, pH 7.6; +25°C: 2.30 x 10-4 mol/L
Michaelis constants (ATP):
Tris buffer, 0.1 mol/L, pH 7.6; +28°C: 1.60 x 10-4 mol/L
Tea buffer, 0.1 mol/L, pH 7.6; +25°C: 1.90 x 10-4 mol/L
Inhibitors: EDTA, SH-blocking compounds, sorbose-1-phosphate, polyphosphates, 6-deoxy-6-fluoroglucose, 2-C-hydroxymethylglucose, lyxose.
Activators: Mg2+, catecholamines
pH optimum: 7.0-10.0 (see figure)
Temperature dependence: See figure
pH stability: 5.0-9.0 (see figure)
Thermal stability: 100% for 20 minutes at +37°C, 50% for 20 minutes at +45°C, 75% for 5 hours at +37°C (see figures)
Specificity: Hexokinase phosphorylates D-glucose, D-fructose, D-mannose, D-glucosamin, 2-deoxyglucose. L-Arabinose, D-xylose, L-rhamnose, D-galactose, D-lactose, sucrose, maltose, trehalose, raffinose, N-acetyl glucosamine do not react. ATP can be partially replaced by other nucleotides.", "Name": "Properties" }, { "Language": "en", "Value": "Appearance: Clear, yellowish solution, in 50% glycerol (v/v)
pH value: 6.0-7.0
Activity (+25°C, glucose): ≥1200 U/mL
Activity (+30°C): ≥1680 U/mL
Protein (Biuret): ≥75 mg/mL
Contaminants (expressed as percentage of Hexokinase activity):
Alcohol dehydrogenase: ≤0.001
ATPase: ≤0.05
Creatine kinase: ≤0.001
G6P-DH: ≤0.005
Glutamate dehydrogenase: ≤0.01
Glutathione reductase: ≤0.005
Myokinase: ≤0.001
6-Phosphogluconate dehydrogenase: ≤0.001
Phophoglucose isomerase: ≤0.002
Phosphoglucomutase: ≤0.02
Glucose: ≤0.125 μg/mg lyophilizate
Glycerol (enzymatic): 560-680 mg/mL ≙ 45-55%
Stability: At +2 to +8°C within specification range for 18 months.", "Name": "Specification" } ] } } ] }

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