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After first use on the instrument, the reagents are stable for up to 8 weeks.", "Name": "Storage Conditions (Working Solution)" }, { "Language": "en", "Value": "
VialContentComposition
GLYBReagent cassette,
250 tests
Reagent: PIPES, 50 mmol/L, pH 6.8; glycerol kinase, ≥175 U/L; glycerol-3-phosphate oxidase, ≥2.5 kU/L; horseradish peroxidase, ≥100 U/L; adenosine triphosphate, 1.4 mmol/L; 4-aminoantipyrine, 0.13 mmol/L; 4-chlorophenol, 4.7 mmol/L; sodium cholate, 0.2 mmol/L; non-reactive surfactant; preservative.
", "Name": "Content" }, { "Language": "en", "Value": "Unit of measure is \"piece\".", "Name": "CB - Order Information" }, { "Language": "en", "Value": "
Test protocol
Glycerol concentrations can be determined in the ranges shown in the table below, depending on the selected test protocol.
Cedex Bio HT test definition
Test definitionGLYB
(747)
GLYD
(794)
GLYE
(127)
Measuring range
     mmol/L
     mg/L
Standard
0.1 – 10
9.21 – 921 
High + dilution
1 – 100
92.1 – 9,210
High extended
10 – 1000(1)
921 – 92,100(1) 
Control levelA 1, 2, 3A 2, 3Custom
Sample predilutionNo1:101:100
Measuring modeAbsorbanceAbsorbanceAbsorbance
Abs. calc. modeEndpointEndpointEndpoint
Reaction modeR-SR-SR-S
Reaction directionIncreaseIncreaseIncrease
Wavelength A/B512/659 nm512/659 nm512/659 nm
Calc. first/last17/4217/4217/42
Unitmmol/L, mg/Lmmol/L, mg/Lmmol/L, mg/L
  1. Up to 10 mol/L  (921 g/L) with automatic predilution.
Pipetting parameters
Pipetting parametersGLYB
(747)
GLYD
(794)
GLYE
(127)
Water Diluent (Sample Predilution) (1)No20 μL sample + 180 μL water2 μL sample + 198 μL water
R120 μL120 μL120 μL
Sample (S)2 μL + 28 μL water2 μL + 28 μL water2 μL + 28 μL water
Total Volume150 μL150 μL150 μL
  1. Sample predilution is performed in a prior step, after which the reaction mix is prepared in the same way as the unmodified sample.
Calibration
Calibration
CalibratorCalibrator A Bio (CAL A, 07-1100-4)
Calibration modeLinear regression
Calibration intervalEach new lot, and if recalibration is required due to QC results.
Quality control
Protocol GLYB
  • Control A Level 1 Bio (CONA1, 07-1101-2)
  • Control A Level 2 Bio (CONA2, 07-1103-9)
  • Control A Level 3 Bio (CONA3, 07-1104-7)
Protocol GLYD
  • Control A Level 2 Bio (CONA2, 07-1103-9)
  • Control A Level 3 Bio (CONA3, 07-1104-7)
Protocol GLYE
  • Custom control: For GLYE, use a higher concentrated custom control solution of glycerol in water in the range of 20 – 75 mmol/L (1.8 – 7 g/L) glycerol.

Use the recommended control material. Other suitable control material can also be used.
Control intervals and acceptance limits should be adapted to each lab\u00adoratory's individual requirements. If values do not fall within the defined limits, corrective measures and recalibration are required.
Conversion factors
Conversion factors for glycerol concentration:
  • 1 mmol/L = 92.09 mg/L
  • 1 g/L = 10.86 mmol/L
Traceability
This method has been standardized against isotope dilution mass spectrometry (ID-MS).
Precision
Representative performance data on Cedex Bio HT Analyzers are shown. Results obtained in individual laboratories may differ. Precision was determined in samples of two concentration levels. Coefficients of variation (CV) were calculated for in-run precision (n=21) and inter-run precision (on 21 days).
 Level 1Level 2
Mean0.97 mmol/L (89.3 mg/L)1.63 mmol/L (150 mg/L)
CV in-run [%]1.61.6
CV inter-run [%]1.91.9
", "Name": "Protocols" }, { "Language": "en", "Value": "This product is intended for quantitative determination of glycerol in aqueous solutions using the Cedex Bio HT Analyzer.", "Name": "Product Description" }, { "Language": "en", "Value": "Measuring Range
Glycerol concentration can be determined in following range:
  • GLYB: 9.21 - 921 mg/L (0.1 - 10 mmol/L)
  • GLYD: 92.1 - 9210 mg/L (1 - 100 mmol/L),
up to 92 g/L (1 mol/L) with automatic dilution

Conversion Factors
Conversion factors for phosphate concentration:
  • 1 mmol/L = 92.09 mg/L
  • 1 mg/L = 0.01086 mmol/L

Traceability
This method has been standardized against against isotope dilution mass spectrometry (ID-MS).

Precision
Representative performance data on Cedex Bio HT analyzers are shown. Results obtained in individual laboratories may differ.
Precision was determined in samples of two concentration levels. Coefficients of variation (CV) were calculated for in-run precision (n=21) and inter-run precision (on 21 days).

 Level 1\t\t\t\t  Level 2
Mean\t\t 0.97 mmol/L
(89.3 mg/L)
1.63 mmol/L
(150 mg/L)
CV in-run1.6 %1.6 %
CV inter-run\t 1.9 %1.9 %
", "Name": "Background Information - Help Corner" }, { "Language": "en", "Value": "
Analyzer and accessories
  • Cedex Bio HT Analyzer*, including accessories and disposables
  • Standard laboratory equipment
Calibrators and Controls
  • Calibrator A Bio*
  • Control A Level 1 Bio*
  • Control A Level 2 Bio*
  • Control A Level 3 Bio*
", "Name": "Additional Equipment and Reagent Required" }, { "Language": "en", "Value": "Glycerol can be used as source of carbon and energy in microbial fermentation. In order to provide sufficient supply and to avoid inhibition by high concentrations, the optimal feeding with glycerol should be according to the actual consumption. For control of culture media and for monitoring in a culture process, the glycerol concentration can be determined on a Cedex Bio or Cedex Bio HT Analyzer with high reliability and convenience.", "Name": "Applications" }, { "Language": "en", "Value": "Method: Enzymatic photometric assay using glycerol kinase and glycerol-3-phosphate oxidase.
Recovery: ±10%", "Name": "Specification" } ] } } ] }

Glycerol Bio HT

Test kit for the Cedex Bio HT Analyzer

Cedex Glycerol

Overview

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Technical documents

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