| Vial | Content | Composition |
|---|---|---|
| GLYB | Reagent cassette, 250 tests | Reagent: PIPES, 50 mmol/L, pH 6.8; glycerol kinase, ≥175 U/L; glycerol-3-phosphate oxidase, ≥2.5 kU/L; horseradish peroxidase, ≥100 U/L; adenosine triphosphate, 1.4 mmol/L; 4-aminoantipyrine, 0.13 mmol/L; 4-chlorophenol, 4.7 mmol/L; sodium cholate, 0.2 mmol/L; non-reactive surfactant; preservative. |
| Test definition | GLYB (747) | GLYD (794) | GLYE (127) |
|---|---|---|---|
| Measuring range mmol/L mg/L | Standard 0.1 – 10 9.21 – 921 | High + dilution 1 – 100 92.1 – 9,210 | High extended 10 – 1000(1) 921 – 92,100(1) |
| Control level | A 1, 2, 3 | A 2, 3 | Custom |
| Sample predilution | No | 1:10 | 1:100 |
| Measuring mode | Absorbance | Absorbance | Absorbance |
| Abs. calc. mode | Endpoint | Endpoint | Endpoint |
| Reaction mode | R-S | R-S | R-S |
| Reaction direction | Increase | Increase | Increase |
| Wavelength A/B | 512/659 nm | 512/659 nm | 512/659 nm |
| Calc. first/last | 17/42 | 17/42 | 17/42 |
| Unit | mmol/L, mg/L | mmol/L, mg/L | mmol/L, mg/L |
| Pipetting parameters | GLYB (747) | GLYD (794) | GLYE (127) |
|---|---|---|---|
| Water Diluent (Sample Predilution) (1) | No | 20 μL sample + 180 μL water | 2 μL sample + 198 μL water |
| R | 120 μL | 120 μL | 120 μL |
| Sample (S) | 2 μL + 28 μL water | 2 μL + 28 μL water | 2 μL + 28 μL water |
| Total Volume | 150 μL | 150 μL | 150 μL |
| Calibration | |
|---|---|
| Calibrator | Calibrator A Bio (CAL A, 07-1100-4) |
| Calibration mode | Linear regression |
| Calibration interval | Each new lot, and if recalibration is required due to QC results. |
| Level 1 | Level 2 | |
|---|---|---|
| Mean | 0.97 mmol/L (89.3 mg/L) | 1.63 mmol/L (150 mg/L) |
| CV in-run [%] | 1.6 | 1.6 |
| CV inter-run [%] | 1.9 | 1.9 |
Test kit for the Cedex Bio HT Analyzer
