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",
"Name": "Content"
},
{
"Language": "English",
"Value": "L-glutamine is deaminated by glutaminase (GLNase) to L-glutamate. L-glutamate is oxidized in the presence of L-glutamate oxidase (GOD) to α-ketoglutarate, ammonia, and hydrogen peroxide, which generates a colored quinone dye in the presence of peroxidase (POD). The formation of the dye is measured photometrically and is directly proportional to the glutamine concentration.
In order to prevent any influence on the result by glutamate in the sample, glutamate oxidase and catalase are added in a prior step to degrade glutamate and H2O2 without dye formation.",
"Name": "Principle"
},
{
"Language": "English",
"Value": "Measuring Range Glutamine concentration can be determined in following ranges:
GLN2B: 15 - 1.500 mg/L (0.1 - 10 mmol/L)
GLN2D: 75 - 7.500 mg/L (0.5 - 50 mmol/L),
up to maximal solubility with automatic dilution
Conversion Factors Conversion factors for glutamine concentration:
1 g/L = 6.842 mmol/L
1 mmol/L = 146.2 mg/L
Traceability This method has been standardized against glutamine net weight.
Precision Representative performance data on Cedex Bio HT Analyzers are shown. Results obtained in individual laboratories may differ. Precision was determined in samples of three concentration levels. Coefficients of variation (CV) were calculated for in-run precision (n=21) and inter-run precision (on 10 days).
Level 1
\t Level 2
Level 3
Mean
1.74 mmol/L (253.8 mg/L)
\t 3.89 mmol/L\t \t (569.2 mg/L)
8.44 mmol/L (1233.7 mg/L)
CV in-run
1.8%
\t 1.8%
1.2%
CV inter-run
2.3%
\t 2.7%
1.9%
",
"Name": "Background Information - Help Corner"
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"Language": "English",
"Value": "Unit of measure is \"piece\".",
"Name": "CB - Order Information"
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{
"Language": "English",
"Value": "
Analyzer and accessories
Cedex Bio HT Analyzer*, including accessories and disposables
Calibration: Calibrator Calibrator B Bio (CAL B, 07-1105-5) Calibration mode\t\t\t\t\t Linear regression Calibration interval\t\t\t\t 84 days, and if recalibration is required due to QC results
Quality control: Control B Level 1 Bio (CONB1, 07-1106-3) Control B Level 2 Bio (CONB2, 07-1107-1) Control B Level 3 Bio (CONB3, 07-1109-8) Use the recommended control material. Other suitable control material can be used in addition. Control intervals and acceptance limits should be adapted to each laboratory's individual requirements. If values do not fall within the defined limits, corrective measures and recalibration are required",
"Name": "Protocols - Help Corner"
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{
"Language": "English",
"Value": "Method: Enzymatic photometric assay using glutaminase. Recovery: ±15%",
"Name": "CB - Specification"
},
{
"Language": "English",
"Value": "Glutamine is an essential nutrient required in cell cultures and microbial fermentation. For optimal cell growth and product formation the glutamine concentration has to be kept whithin an appropriate concentration range, providing sufficient nutrition and avoiding toxical accumulation of ammonia by degradation of an excess of glutamine. Due to the spontaneous degradation, additional glutamine should be added during the cultivation, according to the actual consumption. For control of culture media and for monitoring in a culture process, the glutamine concentration can be determined on a Cedex Bio or Cedex Bio HT Analyzer with high reliability and convenience.",
"Name": "Applications"
},
{
"Language": "English",
"Value": "Method: Enzymatic photometric assay using glutaminase. Recovery: ±15%",
"Name": "Specification"
},
{
"Language": "English",
"Value": "
Precautions
Handle all samples as if potentially infectious, using safe laboratory procedures. As the sensitivity and titer of potential pathogens in the sample material can vary, the operator must optimize pathogen inactivation and follow the appropriate measures according to local safety regulations.
Do not eat, drink, or smoke in the laboratory work area.
Do not pipette by mouth.
Wear protective disposable gloves, laboratory coats, and eye protection, when handling samples and kit reagents.
Wash hands thoroughly after handling samples and reagents.
Waste handling
Discard unused reagents and waste in accordance with country, federal, state, and local regulations.
Safety Data Sheets (SDS) are available online at documentation.roche.com, or upon request from the local Roche office.
",
"Name": "Safety Information"
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For measurement of acute pancreatitis (pancreatic α-amylase) in human serum and urine selective blocking of salivary α-amylase isoenzyme is achieved in the presence of the pancreatic h-α-amylase.Qualified for thecobas platforms.
Product Description
For measurement of acute pancreatitis (pancreatic α-amylase) in human serum and urine selective blocking of salivary α-amylase isoenzyme is achieved in the presence of the pancreatic h-α-amylase.Qualified for thecobas platforms.
Ordering
Name
PackSizePIM360
Description
11543601103
custom fill
MAKM-Tu66C7-IgG(BR)SQ
Notes
Will be supplied as "MAK<H-S-Amy>M-Tu66C7-IgG(BR)SQ". Unit of measure is "g active ingredient".
