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Nomenclature: L-glutamate:NAD(P)
+ oxidoreductase (deaminating)
CAS Number: 9029-11-2
Molecular weight: ~2 200 kD for the associated enzyme with 8 subunits; 280 kD for one subunit.
Michaelis constants (Tris buffer, pH 8.0, +23°C):
L-glutamate: 1.8 x 10
-3 mol/L
NADP: 4.7 x 10
-5 mol/L
α-ketoglutarate: 7.0 x 10
-4 mol/L
NH
4+: 3.2 x 10
-3 mol/L
NADPH: 2.6 x 10
-5 mol/L
Km values for NAD or NADH are difficult to obtain due to their inhibitory action.
Inhibitors: 4-chloromercuribenzoate, Na
2S, diethyldithiocarbamate, 1,10-phenanthroline,
8-hydroxyquinoline, NaN
3, thyroxine, heparin, sulfonylcarbamides, Cu
2+, Hg
2+, Ag
2+, Fe
3+, Zn
2+, K
+, PO
42-, NO
3-Activators: Thioglycolic acid, b-mercaptoethylamine, EDTA, α, α'-dipyridyl
pH optimum: 8.0 (see figure)

pH optimum
Temperature dependence: See figure

Temperature dependence
pH stability: 5.5-6.5 (see figure)

pH stability
Thermal stability: Up to +60°C (see figure)

Thermal stability
Specificity: The oxidation of L-glutamate is stimulated by ADP and inhibited by GTP. In contrast, the oxidation of alanine, leucine, isoleucine, methionine, valine, norleucine, norvaline and 2-aminobutyrates is stimulated by GTP and inhibited by ADP.",
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Appearance: White lyophilizate
Solubility: Clear, colorless solution in water (c=20 mg/mL)
pH value (c=20 mg/L in water): 6.5-7.5
Activity (+25°C, α-ketoglutarat): ≥80 U/mg lyophilizate
Contaminants (expressed as percentage of Glutamate Dehydrogenase activity):
Alcohol dehydrogenase: ≤0.005
Lactate dehydrogenase: ≤0.005
Malate dehydrogenase: ≤0.005
''NADH-Oxidase'': ≤0.005
NH4: ≤0.05 μg/mg lyophilizate
Stability: At +2 to +8°C within specification range for 12 months. Store dry.",
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