{ "ProductData": { "ID": "", "ProductType": "Others", "BrandName": "Glutamate Dehydrogenase (NAD(P))", "ProductNameAddition": "from E.coli overproducer, lyophilizate", "RegulatoryDisclaimer1": "For further processing only.", "DisclaimerGroup1": "", "RegulatoryDisclaimer2": null, "DisclaimerGroup2": null, "RegulatoryDisclaimer3": null, "SampleType": [ ], "LicenseDisclaimers": [ ], "RelatedLinks": "", "Clone": "", "ControlTissue": [ "" ], "ISOtypes": "", "Species": [ "" ], "StainLocalization": [ "" ], "ProductNameGlobal": "Glutamate Dehydrogenase NAD P from E.coli overproducer lyophilizate" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/CB_IMAGE_.png", "ImageType": "Image main" }, "Product2Taxonomy": { "Product2TaxonomyReferences": [ { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeID": "02-0014", "StructureNodeStatus": "valid", "NodeName": "Enzymes" }, { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "NodeID": "70-014-00", "StructureNodeStatus": "valid", "NodeName": "Dehydrogenases" }, { "StructureSystemIdentifier": "OWP_Organization", "StructureSystemName": "Organization", "NodeID": "01-01-01-07-00", "StructureNodeStatus": "valid", "NodeName": "CustomBiotech and LifeScience" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeID": "02-0011", "StructureNodeStatus": "valid", "NodeName": "Clinical Chemistry Raw Materials" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeID": "16-00-00", "StructureNodeStatus": "valid", "NodeName": "Nitrogen Metabolism Testing" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "NodeID": "520", "StructureNodeStatus": "valid", "NodeName": "CustomBiotech" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeID": "180-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "NodeID": "999-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "NodeID": "99-00-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "NodeID": "99-00-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "NodeID": "999", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "NodeID": "99-00-00", "StructureNodeStatus": "invalid", "NodeName": "" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "11745727103", "MaterialDescription": "GlDH,Lyo.,rec.", "RegisteredProductName": "Glutamate Dehydrogenase (NAD(P))", "GTIN": "", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "custom fill", "PackSizeDescPIM360": "Not Available", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"GlDH, Lyo., rec.\". Unit of measure is \"MU\"." } ] }, "Product2Products": { "Product2ProductReference": [ ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Name": "CB - Order Information", "Value": "Will be supplied as \"GlDH, Lyo., rec.\". Unit of measure is \"MU\".", "Language": "en", "Country": "XG", "Code": "CB - Order Information" }, { "Name": "Applications", "Value": "Use recombinant Glutamate Dehydrogenase in diagnostic tests for the determination of ammonia, urea, L-glutamate, glutamate pyruvate transaminase and leucine aminopeptidase.", "Language": "en", "Country": "XG", "Code": "Applications" }, { "Name": "Positioning", "Value": "Recombinant glutamate dehydrogenase.", "Language": "en", "Country": "XG", "Code": "Positioning" }, { "Name": "Properties", "Value": "Nomenclature: L-glutamate:NAD(P)+ oxidoreductase (deaminating)
CAS Number: 9029-11-2
Molecular weight: ~2 200 kD for the associated enzyme with 8 subunits; 280 kD for one subunit.
Michaelis constants (Tris buffer, pH 8.0, +23°C):
L-glutamate: 1.8 x 10-3 mol/L
NADP: 4.7 x 10-5 mol/L
α-ketoglutarate: 7.0 x 10-4 mol/L
NH4+: 3.2 x 10-3 mol/L
NADPH: 2.6 x 10-5 mol/L
Km values for NAD or NADH are difficult to obtain due to their inhibitory action.
Inhibitors: 4-chloromercuribenzoate, Na2S, diethyldithiocarbamate, 1,10-phenanthroline,
8-hydroxyquinoline, NaN3, thyroxine, heparin, sulfonylcarbamides, Cu2+, Hg2+, Ag2+, Fe3+, Zn2+, K+, PO42-, NO3-
Activators: Thioglycolic acid, b-mercaptoethylamine, EDTA, α, α'-dipyridyl
pH optimum: 8.0 (see figure)pH optimum
Temperature dependence: See figureTemperature dependence
pH stability: 5.5-6.5 (see figure)pH stability
Thermal stability: Up to +60°C (see figure)Thermal stability
Specificity: The oxidation of L-glutamate is stimulated by ADP and inhibited by GTP. In contrast, the oxidation of alanine, leucine, isoleucine, methionine, valine, norleucine, norvaline and 2-aminobutyrates is stimulated by GTP and inhibited by ADP.", "Language": "en", "Country": "XG", "Code": "Properties" }, { "Name": "Specification", "Value": "Appearance: White lyophilizate
Solubility: Clear, colorless solution in water (c=20 mg/mL)
pH value (c=20 mg/L in water): 6.5-7.5
Activity (+25°C, α-ketoglutarat): ≥80 U/mg lyophilizate
Contaminants (expressed as percentage of Glutamate Dehydrogenase activity):
Alcohol dehydrogenase: ≤0.005
Lactate dehydrogenase: ≤0.005
Malate dehydrogenase: ≤0.005
''NADH-Oxidase'': ≤0.005
NH4: ≤0.05 μg/mg lyophilizate
Stability: At +2 to +8°C within specification range for 12 months. Store dry.", "Language": "en", "Country": "XG", "Code": "Specification" } ] } } ] }

Glutamate Dehydrogenase (NAD(P))

from E.coli overproducer, lyophilizate


Ordering Information

Technical documents

for certificates, method sheets (instructions for use), safety data sheets and software downloads
error errorMessage
Sorry, we couldn't find the content you are looking for
Please try again later