{ "ProductData": { "ID": "3.1.3.3.7.5", "ProductType": "Others", "BrandName": "Glutamate Dehydrogenase (NAD(P))", "ProductNameAddition": "from beef liver, solution", "RegulatoryDisclaimer1": "For further processing only.", "DisclaimerGroup1": "", "RegulatoryDisclaimer2": null, "DisclaimerGroup2": null, "RegulatoryDisclaimer3": null, "SampleType": [ ], "LicenseDisclaimers": [ ], "RelatedLinks": "", "Clone": "", "ControlTissue": [ "" ], "ISOtypes": "", "Species": [ "" ], "StainLocalization": [ "" ], "ProductNameGlobal": "Glutamate Dehydrogenase NAD P from beef liver solution" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/CB_IMAGE_.png", "ImageType": "Image main" }, "Product2Taxonomy": { "Product2TaxonomyReferences": [ { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeID": "02-0014", "StructureNodeStatus": "valid", "NodeName": "Enzymes" }, { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "NodeID": "70-014-00", "StructureNodeStatus": "valid", "NodeName": "Dehydrogenases" }, { "StructureSystemIdentifier": "OWP_Organization", "StructureSystemName": "Organization", "NodeID": "01-01-01-07-00", "StructureNodeStatus": "valid", "NodeName": "CustomBiotech and LifeScience" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeID": "02-0011", "StructureNodeStatus": "valid", "NodeName": "Clinical Chemistry Raw Materials" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeID": "16-00-00", "StructureNodeStatus": "valid", "NodeName": "Nitrogen Metabolism Testing" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "NodeID": "520", "StructureNodeStatus": "valid", "NodeName": "CustomBiotech" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeID": "180-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "NodeID": "999-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "NodeID": "99-00-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "NodeID": "99-00-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "NodeID": "999", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "NodeID": "99-00-00", "StructureNodeStatus": "invalid", "NodeName": "" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "10120847103", "MaterialDescription": "Glutamate Dehydrogenase, Beef Liver", "RegisteredProductName": "Glutamate Dehydrogenase (NAD(P))", "GTIN": "", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "custom fill", "PackSizeDescPIM360": "Not Available", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"Glutamate Dehydrogenase, Beef Liver\". Unit of measure is \"L\"." } ] }, "Product2Products": { "Product2ProductReference": [ ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Name": "Applications", "Value": "Use Glutamate Dehydrogenase in diagnostic tests for the determination of ammonia, urea, L-glutamate, glutamate pyruvate transaminase and leucine aminopeptidase.", "Language": "en", "Country": "XG", "Code": "Applications" }, { "Name": "CB - Order Information", "Value": "Will be supplied as \"Glutamate Dehydrogenase, Beef Liver\". Unit of measure is \"L\".", "Language": "en", "Country": "XG", "Code": "CB - Order Information" }, { "Name": "Positioning", "Value": "Dehydrogenase that catalyzes the conversion of glutamate to α-ketoglutarate.", "Language": "en", "Country": "XG", "Code": "Positioning" }, { "Name": "Properties", "Value": "Nomenclature: L-glutamate:NAD(P)⁺ oxidoreductase (deaminating)
CAS Number: 9029-11-2
Molecular weight: ~2 200 kD for the associated enzyme with 8 subunits; 280 kD for one subunit.
Michaelis constants (Tris buffer, pH 8.0, +23°C):
L-glutamate: 1.8 x 10^-3 mol/L
NADP: 4.7 x 10^-5 mol/L
α-ketoglutarate: 7.0 x 10^-4 mol/L
NH₄⁺: 3.2 x 10^-3 mol/L
NADPH: 2.6 x 10^-5 mol/L
Km values for NAD or NADH are difficult to obtain due to their inhibitory action.
Inhibitors: 4-chloromercuribenzoate, Na₂S, diethyldithiocarbamate, 1,10-phenanthroline,
8-hydroxyquinoline, NaN₃, thyroxine, heparin, sulfonylcarbamides, Cu²⁺, Hg²⁺, Ag²⁺, Fe³⁺, Zn²⁺, K⁺, PO₄^2-, NO₃^-
Activators: Thioglycolic acid, b-mercaptoethylamine, EDTA, α, α'-dipyridyl
pH optimum: 8.0 (see figure)pH optimum
Temperature dependence: See figureTemperature dependence
pH stability: 5.5-6.5 (see figure)pH stability
Thermal stability: Up to +60°C (see figure)Thermal stability
Specificity: The oxidation of L-glutamate is stimulated by ADP and inhibited by GTP. In contrast, the oxidation of alanine, leucine, isoleucine, methionine, valine, norleucine, norvaline and 2-aminobutyrates is stimulated by GTP and inhibited by ADP.
Remarks: GIDH suspension or solution can be dialyzed against phosphate buffer, 10 mmol/L. GIDH molecules have the tendency to associate in some test formulations, modified GIDH minimizes this effect.", "Language": "en", "Country": "XG", "Code": "Properties" }, { "Name": "Specification", "Value": "Appearance: Clear, colourless solution in glycerol
pH value: 7.0-7.8
Specific Activity: ≥120 U/mg
Protein (Biuret): 30±3 mg/mL
Contaminants (expressed as percentage of Glutamate Dehydrogenase activity):
Alcohol dehydrogenase: ≤0.01
Lactate dehydrogenase: ≤0.01
Malate dehydrogenase: ≤0.01
NH₄: ≤0.16 μg/mg protein
Glycerol: 560-680 mg/mL (45-55% (v/v))
EDTA (complexometric): 12.2-13.4 mmol/L
pH 5.5 treatment (30 minutes): Corresponds to specification
Stability: At +2 to +8°C within specification range for 18 months.", "Language": "en", "Country": "XG", "Code": "Specification" } ] } } ] }