{ "ProductData": { "RelatedLinks": "", "RegulatoryDisclaimer1": "For further processing only.", "RegulatoryDisclaimer2": "", "StainLocalization": [ "" ], "ProductType": "Others", "DisclaimerGroup2": null, "ProductNameGlobal": "Glutamate Dehydrogenase NAD P from beef liver solution", "DisclaimerGroup1": "Non-IVD", "ControlTissue": [ "" ], "BrandName": "Glutamate Dehydrogenase (NAD(P))", "LicenseDisclaimers": [ ], "Isotypes": "", "RegulatoryDisclaimer3": "", "ID": "3.1.3.3.7.5", "ProductNameAddition": "from beef liver, solution", "Species": [ "" ], "Clone": "" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/CB_IMAGE.png", "ImageType": "Image main" }, "Product2Taxomony": { "Product2TaxomonyReferences": [ { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "Enzymes", "NodeID": "02-0014" }, { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "NodeName": "Dehydrogenases", "NodeID": "70-014-00" }, { "StructureSystemIdentifier": "OWP_Organization", "StructureSystemName": "Organization", "NodeName": "CustomBiotech and LifeScience", "NodeID": "01-01-01-07-00" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "Clinical Chemistry Raw Materials", "NodeID": "02-0011" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeName": "Nitrogen Metabolism Testing", "NodeID": "16-00-00" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "NodeName": "CustomBiotech", "NodeID": "520" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeName": "Manufacturing", "NodeID": "180-00" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "NodeName": "NOT APPLICABLE", "NodeID": "999-00" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "NodeName": "NOT APPLICABLE", "NodeID": "999" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "10120847103", "MaterialDescription": "Glutamate Dehydrogenase, Beef Liver", "RegisteredProductName": "Glutamate Dehydrogenase (NAD(P))", "GTIN": "", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "custom fill", "PackSizeDescPIM360": "", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"Glutamate Dehydrogenase, Beef Liver\". Unit of measure is \"L\"." } ] }, "Product2Products": { "Product2ProductReference": [ ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Language": "en", "Value": "Use Glutamate Dehydrogenase in diagnostic tests for the determination of ammonia, urea, L-glutamate, glutamate pyruvate transaminase and leucine aminopeptidase.", "Name": "Applications" }, { "Language": "en", "Value": "Will be supplied as \"Glutamate Dehydrogenase, Beef Liver\". Unit of measure is \"L\".", "Name": "CB - Order Information" }, { "Language": "en", "Value": "Dehydrogenase that catalyzes the conversion of glutamate to α-ketoglutarate.", "Name": "Positioning" }, { "Language": "en", "Value": "Nomenclature: L-glutamate:NAD(P)+ oxidoreductase (deaminating)
CAS Number: 9029-11-2
Molecular weight: ~2 200 kD for the associated enzyme with 8 subunits; 280 kD for one subunit.
Michaelis constants (Tris buffer, pH 8.0, +23°C):
L-glutamate: 1.8 x 10-3 mol/L
NADP: 4.7 x 10-5 mol/L
α-ketoglutarate: 7.0 x 10-4 mol/L
NH4+: 3.2 x 10-3 mol/L
NADPH: 2.6 x 10-5 mol/L
Km values for NAD or NADH are difficult to obtain due to their inhibitory action.
Inhibitors: 4-chloromercuribenzoate, Na2S, diethyldithiocarbamate, 1,10-phenanthroline,
8-hydroxyquinoline, NaN3, thyroxine, heparin, sulfonylcarbamides, Cu2+, Hg2+, Ag2+, Fe3+, Zn2+, K+, PO42-, NO3-
Activators: Thioglycolic acid, b-mercaptoethylamine, EDTA, α, α'-dipyridyl
pH optimum: 8.0 (see figure)
Temperature dependence: See figure
pH stability: 5.5-6.5 (see figure)
Thermal stability: Up to +60°C (see figure)
Specificity: The oxidation of L-glutamate is stimulated by ADP and inhibited by GTP. In contrast, the oxidation of alanine, leucine, isoleucine, methionine, valine, norleucine, norvaline and 2-aminobutyrates is stimulated by GTP and inhibited by ADP.
Remarks: GIDH suspension or solution can be dialyzed against phosphate buffer, 10 mmol/L. GIDH molecules have the tendency to associate in some test formulations, modified GIDH minimizes this effect.", "Name": "Properties" }, { "Language": "en", "Value": "Appearance: Clear, colourless solution in glycerol
pH value: 7.0-7.8
Specific Activity: ≥120 U/mg
Protein (Biuret): 30±3 mg/mL
Contaminants (expressed as percentage of Glutamate Dehydrogenase activity):
Alcohol dehydrogenase: ≤0.01
Lactate dehydrogenase: ≤0.01
Malate dehydrogenase: ≤0.01
NH4: ≤0.16 μg/mg protein
Glycerol: 560-680 mg/mL (45-55% (v/v))
EDTA (complexometric): 12.2-13.4 mmol/L
pH 5.5 treatment (30 minutes): Corresponds to specification
Stability: At +2 to +8°C within specification range for 18 months.", "Name": "Specification" } ] } } ] }

Glutamate Dehydrogenase (NAD(P))

from beef liver, solution

logo

Overview

Ordering

Technical documents

error errorMessage
Sorry, we couldn't find the content you are looking for
Please try again later