| Vial | Content | Composition |
|---|---|---|
| GLC3B | Reagent cassette, 800 tests | Reagent 1 (R1): MES, 5.0 mmol/L; adenosine triphosphate, ≥4.5 mmol/L; Mg2+, 24 mmol/L; NADP, ≥7 mmol/L; pH 6.0. |
| Start Reagent (SR): HEPES, 200 mmol/L; Mg2+, 4 mmol/L; hexokinase (HK) (yeast), ≥300 μkat/L; G-6-PDH (E. coli), ≥300 μkat/L; pH 8.0. |
| Test definition | GLC3L (081) | GLC3B (938) | GLC3D (713) |
|---|---|---|---|
| Measuring range mmol/L mg/L | Low 0.022 – 4.16 4 – 750 | Standard 0.111 – 41.6 20 – 7,500 | High + dilution 1.11 – 416(1) 200 – 75,000(1) |
| Cntrol level | A 1 | A 1, 2, 3 | A 2, 3 |
| Sample predilution | No | No | 1:10 |
| Measuring mode | Absorbance | Absorbance | Absorbance |
| Abs. calc. mode | Endpoint | Endpoint | Endpoint |
| Reaction mode | R1-S-SR | R1-S-SR | R1-S-SR |
| Reaction direction | Increase | Increase | Increase |
| Wavelength A/B | 340/652 nm | 340/652 nm | 340/652 nm |
| Calc. first/last | 33/69 | 33/69 | 33/69 |
| Unit | mmol/L, mg/L | mmol/L, mg/L | mmol/L, mg/L |
| Pipetting parameters | GLC3L (081) | GLC3B (938) | GLC3D (713) |
|---|---|---|---|
| Water Diluent (Sample Predilution)(1) | No | No | 20 µL sample + 180 µL water |
| R1 | 28 μL + 125 μL water | 28 μL + 125 μL water | 28 μL + 125 μL water |
| Sample (S) | 20 μL + 10 μL water | 2 μL + 16 μL water | 2 μL + 16 μL water |
| SR | 10 μL + 8 μL water | 10 μL + 20 μL water | 10 μL + 20 μL water |
| Total Volume | 201 µL | 201 μL | 201 μL |
| Calibration | |
|---|---|
| Calibrator | Calibrator A Bio (CAL A, 07-1100-4) |
| Calibration mode | Linear regression |
| Calibration interval | Each new lot, and if recalibration is required due to QC results. |
| Level 1 | Level 2 | Level 3 | |
|---|---|---|---|
| Mean | 3.57 mmol/L (643 mg/L) | 13.7 mmol/L (2.470 mg/L) | 36.9 mmol/L (6.650 mg/L) |
| CV in-run [%] | 0.7 | 0.6 | 0.7 |
| CV inter-run [%] | 1.3 | 1.2 | 1.3 |
Test kit for the Cedex Bio HT Analyzer
