{ "ProductData": { "RelatedLinks": "", "RegulatoryDisclaimer1": "For further processing only.", "RegulatoryDisclaimer2": "", "StainLocalization": [ "" ], "ProductType": "Others", "DisclaimerGroup2": null, "ProductNameGlobal": "Glucose-6-Phosphate Dehydrogenase G6P-DH lyophilizate", "DisclaimerGroup1": "Non-IVD", "ControlTissue": [ "" ], "BrandName": "Glucose-6-Phosphate Dehydrogenase (G6P-DH)", "LicenseDisclaimers": [ ], "Isotypes": "", "RegulatoryDisclaimer3": "", "ID": "3.1.1.3.16.5", "ProductNameAddition": "from Leuconostoc mesenteroides, expressed in E. coli, lyophilizate", "Species": [ "" ], "Clone": "" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/CB_IMAGE.png", "ImageType": "Image main" }, "Product2Taxomony": { "Product2TaxomonyReferences": [ { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "Enzymes", "NodeID": "02-0014" }, { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "NodeName": "Dehydrogenases", "NodeID": "70-014-00" }, { "StructureSystemIdentifier": "OWP_Organization", "StructureSystemName": "Organization", "NodeName": "CustomBiotech and LifeScience", "NodeID": "01-01-01-07-00" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "Clinical Chemistry Raw Materials", "NodeID": "02-0011" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeName": "Carbohydrate Testing", "NodeID": "01-00-00" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeName": "Cardiac Testing", "NodeID": "02-00-00" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeName": "Enzyme Testing", "NodeID": "06-00-00" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "NodeName": "CustomBiotech", "NodeID": "520" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeName": "Manufacturing", "NodeID": "180-00" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "NodeName": "NOT APPLICABLE", "NodeID": "999-00" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "NodeName": "NOT APPLICABLE", "NodeID": "999" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "11293206103", "MaterialDescription": "Glucose-6-phosphate Dehydrogenase", "RegisteredProductName": "Glucose-6-Phosphate Dehydrogenase (G6P-DH)", "GTIN": "14038377155507", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "custom fill", "PackSizeDescPIM360": "", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"G6P-DH, rec., Lyo\". Unit of measure is \"MU\"." } ] }, "Product2Products": { "Product2ProductReference": [ ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Language": "en", "Value": "Will be supplied as \"G6P-DH, rec., Lyo\". Unit of measure is \"MU\".", "Name": "CB - Order Information" }, { "Language": "en", "Value": "Use Glucose-6-phosphate Dehydrogenase for the determination of blood glucose or creatine kinase.", "Name": "Applications" }, { "Language": "en", "Value": "Dehydrogenase, that catalyzes the oxidation of Glucose-6-phosphate.", "Name": "Positioning" }, { "Language": "en", "Value": "Nomenclature: D-glucose-6-phosphate:NAD(P)+ 1-oxidoreductase
CAS Number: 9001-40-5
Molecular weight: 110 kD (1) (2 identical subunits 55,000 D)
Isoelectric point: pH 4.6
Michaelis constants (Tris: 0.1 mol/L; pH 7.8, +25°C):
NAD: 1.4 x 10-4 mmol/L
NADP: 3.7 x 10-5 mmol/L
Glucose-6-P: 3.7 x 10-4 mmol/L (NAD as coenzyme)
Glucose-6-P: 2.0 x 10-4 mmol/L (NADP as coenzyme)
Activators/inhibitors:
Phosphate, 5 mmol/L: 100% (NAD), 80% (NADP)
Phosphate, 50 mmol/L: 100% (NAD), 80% (NADP)
Without Mg2+: 90% (NAD), 80% (NADP)
Mg2+, 3 mmol/L: 100% (NAD), 100% (NADP)
Mg2+, 30 mmol/L: 100% (NAD), 100% (NADP)
HCO3-, 3 mmol/L: 100% (NAD), 100% (NADP)
Inhibitors: NADPH is a competitive inhibitor in the NAD-dependent reaction. Unlike the yeast enzyme, myristic acid, dehydroepiandrosterone and palmitoyl CoA do not inhibit.
pH optimum: 7.8 (see figure)
Temperature dependence: See figure
pH stability: 5.0-10.0 (see figure)
Thermal stability: Up to +40°C for native G6P-DH, up to +50°C for modified G6P-DH (see figure)
Buffer stability: Temperature stability can be significantly improved by the increase of ionic strength (see figure).
Stability of the lyophilizate: 100% residual activity after 3 weeks at +35°C
Specificity: G6P-DH is highly specific for glucose-6-phosphate and does not react with fructose-6-P, fructose-1,6-P2 or glucose-1P. 2-Deoxyglucose-6-P is slowly oxidized with NAD (5%) and with NADP (4%).", "Name": "Properties" }, { "Language": "en", "Value": "Appearance: White or slightly yellowish lyophilizate
Solubility: Clear, colorless solution in water (c=10 mg/mL)
pH value (c=10 mg/mL, water): 6.5-7.5
Activity (+25°C, glucose-6-P, NAD): ≥600 U/mg lyophilizate
Specific activity (+25°C): ≥800 U/mg protein
Activity (+30°C): ≥750 U/mg lyophilizate
Activity (+37°C): ≥1,000 U/mg lyophilizate
Activity (+25°C, glucose-6-P, NADP ): No limit
Protein (Biuret): 0.7-0.9 mg/mg lyophilizate
Contaminants (expressed as percentage of Glucose-6-phosphate dehydrogenase activity):
ATPase: ≤0.02
Creatine kinase: ≤0.001
Glutamate dehydrogenase: ≤0.01
Glutathione reductase: ≤0.001
Hexokinase and GIucose dehydrogenase: ≤0.05
Myokinase: ≤0.001
''NADH oxidase'': ≤0.02
''NADPH oxidase'': ≤0.0005
6-Phosphogluconate dehydrogenase (NAD as coenzyme): ≤0.001
6-Phosphogluconate dehydrogenase (NADP as coenzyme): ≤0.001
Phophoglucose isomerase : ≤0.01
Phosphoglucomutase: ≤0.001
Stability: At +2 to +8°C within specification range for 12 months. Store dry.", "Name": "Specification" } ] } } ] }

Glucose-6-Phosphate Dehydrogenase (G6P-DH)

from Leuconostoc mesenteroides, expressed in E. coli, lyophilizate

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