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"BrandName": "Glucose-6-Phosphate Dehydrogenase (G6P-DH)",
"ProductNameAddition": "from
Leuconostoc mesenteroides, expressed in
E. coli, lyophilizate",
"RegulatoryDisclaimer1": "For further processing only.",
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""
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"Name": "CB - Order Information",
"Value": "Will be supplied as \"G6P-DH, rec., Lyo\". Unit of measure is \"MU\".",
"Language": "en",
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"Code": "CB - Order Information"
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"Value": "Dehydrogenase, that catalyzes the oxidation of Glucose-6-phosphate.",
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{
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Nomenclature: D-glucose-6-phosphate:NAD(P)
+ 1-oxidoreductase
CAS Number: 9001-40-5
Molecular weight: 110 kD (1) (2 identical subunits 55,000 D)
Isoelectric point: pH 4.6
Michaelis constants (Tris: 0.1 mol/L; pH 7.8, +25°C)
:NAD: 1.4 x 10
-4 mmol/L
NADP: 3.7 x 10
-5 mmol/L
Glucose-6-P: 3.7 x 10
-4 mmol/L (NAD as coenzyme)
Glucose-6-P: 2.0 x 10
-4 mmol/L (NADP as coenzyme)
Activators/inhibitors:Phosphate, 5 mmol/L: 100% (NAD), 80% (NADP)
Phosphate, 50 mmol/L: 100% (NAD), 80% (NADP)
Without Mg
2+: 90% (NAD), 80% (NADP)
Mg
2+, 3 mmol/L: 100% (NAD), 100% (NADP)
Mg
2+, 30 mmol/L: 100% (NAD), 100% (NADP)
HCO
3-, 3 mmol/L: 100% (NAD), 100% (NADP)
Inhibitors: NADPH is a competitive inhibitor in the NAD-dependent reaction. Unlike the yeast enzyme, myristic acid, dehydroepiandrosterone and palmitoyl CoA do not inhibit.
pH optimum: 7.8 (see figure)

pH optimum
Temperature dependence: See figure

Temperature dependence
pH stability: 5.0-10.0 (see figure)

pH stability
Thermal stability: Up to +40°C for native G6P-DH, up to +50°C for modified G6P-DH (see figure)

Thermal stability
Buffer stability: Temperature stability can be significantly improved by the increase of ionic strength (see figure).

Buffer stability
Stability of the lyophilizate: 100% residual activity after 3 weeks at +35°C
Specificity: G6P-DH is highly specific for glucose-6-phosphate and does not react with fructose-6-P, fructose-1,6-P2 or glucose-1P. 2-Deoxyglucose-6-P is slowly oxidized with NAD (5%) and with NADP (4%).",
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"Value": "
Appearance: White or slightly yellowish lyophilizate
Solubility: Clear, colorless solution in water (c=10 mg/mL)
pH value (c=10 mg/mL, water): 6.5-7.5
Activity (+25°C, glucose-6-P, NAD): ≥600 U/mg lyophilizate
Specific activity (+25°C): ≥800 U/mg protein
Activity (+30°C): ≥750 U/mg lyophilizate
Activity (+37°C): ≥1,000 U/mg lyophilizate
Activity (+25°C, glucose-6-P, NADP ): No limit
Protein (Biuret): 0.7-0.9 mg/mg lyophilizate
Contaminants (expressed as percentage of Glucose-6-phosphate dehydrogenase activity):
ATPase: ≤0.02
Creatine kinase: ≤0.001
Glutamate dehydrogenase: ≤0.01
Glutathione reductase: ≤0.001
Hexokinase and GIucose dehydrogenase: ≤0.05
Myokinase: ≤0.001
''NADH oxidase'': ≤0.02
''NADPH oxidase'': ≤0.0005
6-Phosphogluconate dehydrogenase (NAD as coenzyme): ≤0.001
6-Phosphogluconate dehydrogenase (NADP as coenzyme): ≤0.001
Phophoglucose isomerase : ≤0.01
Phosphoglucomutase: ≤0.001
Stability: At +2 to +8°C within specification range for 12 months. Store dry.",
"Language": "en",
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}