| Test definition | FORB (176) | FORD (181) |
|---|---|---|
| Measuring range mmol/L mg/L | Standard 0.2 – 20 9.0 – 900 | High 2.0 – 200(1) 90 – 9,000(1) |
| Control level | D 1, 2, 3 | D 2, 3 |
| Sample predilution | No | 1:10 |
| Measuring mode | Absorbance | Absorbance |
| Abs. calc. mode | Endpoint | Endpoint |
| Reaction mode | R1-R2-S | R1-R2-S |
| Reaction direction | Increase | Increase |
| Wavelength A/B | 340/409 nm | 340/409 nm |
| Calc. first/last | 18/70 | 18/70 |
| Unit | mmol/L, mg/L | mmol/L, mg/L |
| Pipetting parameters | FORB (176) | FORD (181) |
|---|---|---|
| Water Diluent (Sample Predilution)(1) | No | 20 μL sample + 180 μL water |
| R1 | 100 μL + 5 μL water | 100 μL + 5 μL water |
| R2 | 70 μL + 5 μL water | 70 μL + 5 μL water |
| Sample (S) | 4.5 μL + 18 μL water | 4.5 μL + 18 μL water |
| Total Volume | 202.5 μL | 202.5 μL |
| Calibration | |
|---|---|
| Calibrator | Calibrator D Bio (CAL D, 07-1141-1) |
| Calibration mode | Linear regression |
| Calibration interval | 140 days, and if recalibration is required due to QC results. |
| Level 1 | Level 2 | Level 3 | |
|---|---|---|---|
| Mean | 1.0 mmol/L (45 mg/L) | 8.0 mmol/L (360 mg/L) | 15.0 mmol/L (675 mg/L) |
| CV in-run [%] | 0.8 | 0.3 | 0.4 |
| CV inter-run [%] | 1.9 | 1.9 | 2.1 |
| Vial | Content | Composition |
|---|---|---|
| FORB | Reagent cassette, 200 tests | Reagent 1 (R1): TRIS, 100 mmol/L; sodium chloride, 154 mmol/L; formate dehydrogenase (FDH), approximately 20 U/mL; stabilizer; preservative; pH 7.5. |
| Reagent 2 (R2): Nicotinamide adenine dinucleotide, 60 mmol/L; preservative; pH 4.6. |
Test kit for the Cedex Bio HT Analyzer
