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"Health_Topics", "NameofStructureSystem": "Health Topics", "StructureNodeID": "99-00-00", "StructureGroupPath": "NOT APPLICABLE", "StructureGroupName": "NOT APPLICABLE" } ] } ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Language": "en", "Value": "Store the reagent cassette at +2 to +8°C.", "Name": "Storage Conditions (Product)" }, { "Language": "en", "Value": "Keep at +2 to +15°C for on-board use. After first use on the analyzer, the kit is stable for up to 20 weeks on board.", "Name": "Storage Conditions (Working Solution)" }, { "Language": "en", "Value": "
Analyzer and accessories
  • Cedex Bio HT Analyzer*, including accessories and disposables
  • Standard laboratory equipment
Calibrators and Controls
  • Calibrator D Bio*
  • Control D Level 1 Bio*
  • Control D Level 2 Bio*
  • Control D Level 3 Bio*
", "Name": "Additional Equipment and Reagent Required" }, { "Language": "en", "Value": "
Test protocol
Cedex Bio HT test definition
Test definitionFORB
(176)
FORD
(181)
Measuring range
     mmol/L
     mg/L
Standard
0.2 – 20
9.0 – 900
High
2.0 – 200(1)
90 – 9,000(1)
Control levelD 1, 2, 3D 2, 3
Sample predilutionNoNo
Measuring modeAbsorbanceAbsorbance
Abs. calc. modeEndpointEndpoint
Reaction modeR1-R2-SR1-R2-S
Reaction directionIncreaseIncrease
Wavelength A/B340/409 nm340/409 nm
Calc. first/last18/7018/70
Unitmmol/L, mg/Lmmol/L, mg/L
  1. Up to 2 mol/L (90 g/L) with automatic predilution.
Pipetting parameters
Pipetting parametersFORB
(176)
FORD
(181)
Water Diluent (Sample Predilution)NoNo
R1100 μL + 5 μL water100 μL + 5 μL water
R270 μL + 5 μL water70 μL + 5 μL water
Sample (S)4.5 μL + 18 μL water4.5 μL + 18 μL water
Total Volume202.5 μL202.5 μL
Calibration
Calibration
CalibratorCalibrator D Bio (CAL D, 07-1141-1)
Calibration modeLinear regression
Calibration interval140 days, and if recalibration is required due to QC results.
Quality control
Protocol FORB
  • Control D Level 1 Bio (COND1, 07-1143-8)
  • Control D Level 2 Bio (COND2, 07-1144-6)
  • Control D Level 3 Bio (COND3, 07-1145-4)
Protocol FORD
  • Control D Level 2 Bio (COND2, 07-1144-6)
  • Control D Level 3 Bio (COND3, 07-1145-4)

Use the recommended control material. Other suitable control material can also be used.
Control intervals and acceptance limits should be adapted to each lab\u00adoratory's individual requirements. If values do not fall within the defined limits, corrective measures and recalibration are required.
Conversion factors
Conversion factors for formate concentration:
  • 1 mmol/L = 45.02 mg/L
  • 1 g/L = 22.21 mmol/L
Traceability
This method has been standardized against net weight.
Precision
Representative performance data on Cedex Bio HT Analyzers are shown. Results obtained in individual laboratories may differ. Precision was determined in samples of three concentration levels. Coefficients of variation (CV) were calculated for in-run precision (n=21) and inter-run precision (on 10 days).
 Level 1Level 2Level 3
Mean1.0 mmol/L (45 mg/L)8.0 mmol/L (360 mg/L)15.0 mmol/L (675 mg/L)
CV in-run [%]0.80.30.4
CV inter-run [%]1.91.92.1
", "Name": "Protocols" }, { "Language": "en", "Value": "This product is intended for quantitative determination of formate (formic acid) in aqueous solutions using the Cedex Bio HT Analyzer.", "Name": "Product Description" }, { "Language": "en", "Value": "Measuring Range
Formate concentration can be determined in following range:
  • FORB: 9.0 to 900 mg/L (0.2 to 20 mmol/L)
  • FORD: 90 to 9000 mg/L (2.0 to 200 mmol/L), up to 90 g/L (2 mol/L) with automatic predilution.

Conversion Factors
Conversion factors for formate concentration:
  • 1 mmol/L = 45.02 mg/L
  • 1 g/L = 22.21 mmol/L

Traceability
This method has been standardized against net weight.

Precision
Representative performance data on Cedex Bio HT Analyzers are shown. Results obtained in individual laboratories may differ. Precision was determined in samples of three concentration levels. Coefficients of variation (CV) were calculated for in-run precision (n=21) and inter-run precision (on 10 days).

  Level 1 Level 2 Level 3
Mean 1.0mmol/L
(45 mg/L)     
8.0 mmol/L
(360mg/L)        
15.0 mmol/L
(675 mg/L)
CV in-run         0.8 % 0.3 % 0.4 %
CV inter-run 1.9 % 1.9 % 2.1 %
", "Name": "Background Information - Help Corner" }, { "Language": "en", "Value": "Unit of measure is \"piece\".", "Name": "CB - Order Information" }, { "Language": "en", "Value": "
VialContentComposition
FORBReagent cassette,
200 tests
Reagent 1 (R1): TRIS, 100 mmol/L; sodium chloride, 154 mmol/L; formate dehydrogenase (FDH), approximately 20 U/mL; stabilizer; preservative; pH 7.5.
Reagent 2 (R2): Nicotinamide adenine dinucleotide, 60 mmol/L; preservative; pH 4.6.
", "Name": "Content" }, { "Language": "en", "Value": "Formate is a relevant metabolyte in microbial fermentation. In a controlled fermentation process, formate should be used by the microbes as source of carbon and energy and should not be accumulated in the medium. For monitoring in a culture process, formate can be determined on a Cedex Bio or Cedex Bio HT Analyzer with high reliability and convenience.", "Name": "Applications" }, { "Language": "en", "Value": "Method: Enzymatic photometric assay using formate dehydrogenase.
Recovery: ±15%", "Name": "Specification" } ] } } ] }

Formate Bio HT

Test kit for the Cedex Bio HT Analyzer

Cedex Formate

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Technical documents

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