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Fluorescein-12-dUTP replaces dTTP in the random-primed DNA labeling reaction or in nick translation reactions, as well as in PCR. The nucleotide also serves as a substrate for Terminal Transferase in 3'-end labeling. Fluorescein-labeled probes can be used for in situ hybridization with direct fluorescence detection and detection by ELISA using Anti-Fluorescein-AP, Fab fragments. Repeated fluorescence labeling using Tetramethylrhodamine-6-dUTP (red) and AMCA-6-dUTP (bright blue) is possible.",
"Language": "en",
"Country": "XG",
"Code": "Applications"
},
{
"Name": "CB - Order Information",
"Value": "Will be supplied as ''Fluorescein-12-dUTP''. Unit of measure is ''nmol''. For other than the listed concentrations please inquire.",
"Language": "en",
"Country": "XG",
"Code": "CB - Order Information"
},
{
"Name": "Properties",
"Value": "Nomenclature: Fluorescein-12-deoxyuridine-5'-triphosphate CAS Number: 134344-32-4 Formula: C39H37N4O21P3Li4 Molecular weight: 1018.4 D",
"Language": "en",
"Country": "XG",
"Code": "Properties"
},
{
"Name": "Specification",
"Value": "Appearance: Clear, yellow solution Fluorescein-12-dUTP (1 μmol ≙ 63.3 A495 units, phosphate buffer, 0.1 mol/L, pH 9.0): 1.0-1.1 mmol/L Purity (HPLC, including isomere): 85.0-100.0 area% Function test using In Situ Cell Death Detection Kit, Fluorescein (in situ hybridization): Corresponds to reference Stability: At -15 to -25°C within specification range for 12 months. Store dry. Protect from light.",
"Language": "en",
"Country": "XG",
"Code": "Specification"
}
]
}
}
]
}
