{ "ProductData": { "ID": "3.6.13.2.1.1", "ProductType": "Others", "BrandName": "Fluorescein-12-dUTP", "ProductNameAddition": "1 mM solution", "RegulatoryDisclaimer1": "For further processing only.", "DisclaimerGroup1": "", "RegulatoryDisclaimer2": "", "DisclaimerGroup2": null, "RegulatoryDisclaimer3": "", "SampleType": [ ], "LicenseDisclaimers": [ ], "RelatedLinks": "", "Clone": "", "ControlTissue": [ "" ], "ISOtypes": "", "Species": [ "" ], "StainLocalization": [ "" ], "ProductNameGlobal": "Fluorescein-12-dUTP 1 mM solution" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/CB_IMAGE.png", "ImageType": "Image main" }, "Product2Taxonomy": { "Product2TaxonomyReferences": [ { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "StructureNodeStatus": "valid", "NodeName": "Labeled Nucleotides", "NodeID": "70-030-00" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "StructureNodeStatus": "valid", "NodeName": "Molecular Raw Materials", "NodeID": "02-0136" }, { "StructureSystemIdentifier": "OWP_Organization", "StructureSystemName": "Organization", "StructureNodeStatus": "valid", "NodeName": "CustomBiotech and LifeScience", "NodeID": "01-01-01-07-00" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "StructureNodeStatus": "valid", "NodeName": "RT-PCR/qRT-PCR", "NodeID": "300-00" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "StructureNodeStatus": "valid", "NodeName": "CustomBiotech", "NodeID": "520" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "180-00" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "999" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "99-00-00" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "11375601103", "MaterialDescription": "Fluorescein-12-dUTP", "RegisteredProductName": "Fluorescein-12-dUTP", "GTIN": "", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "custom fill", "PackSizeDescPIM360": "Not Available", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as ''Fluorescein-12-dUTP''. Unit of measure is ''nmol''.
For other than the listed concentrations please inquire." } ] }, "Product2Products": { "Product2ProductReference": [ ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Language": "en", "Value": "Enzymatic nonradioactive labeling reagent for cDNA synthesis, PCR, random primed labeling, nick-translation or primer extension.", "Country": "XG", "Code": "Positioning", "Name": "Positioning" }, { "Language": "en", "Value": "Use Fluorescein-12-dUTP as a substrate for:
  • Terminal Transferase
  • DNA polymerase I (holoenzyme and Klenow fragment)
  • Taq DNA polymerase
  • Reverse transcriptase (e.g., from AMV and M-MuLV)
Fluorescein-12-dUTP replaces dTTP in the random-primed DNA labeling reaction or in nick translation reactions, as well as in PCR. The nucleotide also serves as a substrate for Terminal Transferase in 3'-end labeling.
Fluorescein-labeled probes can be used for in situ hybridization with direct fluorescence detection and detection by ELISA using Anti-Fluorescein-AP, Fab fragments.
Repeated fluorescence labeling using Tetramethylrhodamine-6-dUTP (red) and AMCA-6-dUTP (bright blue) is possible.", "Country": "XG", "Code": "Applications", "Name": "Applications" }, { "Language": "en", "Value": "Will be supplied as ''Fluorescein-12-dUTP''. Unit of measure is ''nmol''.
For other than the listed concentrations please inquire.", "Country": "XG", "Code": "CB - Order Information", "Name": "CB - Order Information" }, { "Language": "en", "Value": "Nomenclature: Fluorescein-12-deoxyuridine-5'-triphosphate
CAS Number: 134344-32-4
Formula: C39H37N4O21P3Li4
Molecular weight: 1018.4 D", "Country": "XG", "Code": "Properties", "Name": "Properties" }, { "Language": "en", "Value": "Appearance: Clear, yellow solution
Fluorescein-12-dUTP (1 μmol ≙ 63.3 A495 units, phosphate buffer, 0.1 mol/L, pH 9.0): 1.0-1.1 mmol/L
Purity (HPLC, including isomere): 85.0-100.0 area%
Function test using In Situ Cell Death Detection Kit, Fluorescein (in situ hybridization): Corresponds to reference
Stability: At -15 to -25°C within specification range for 12 months. Store dry. Protect from light.", "Country": "XG", "Code": "Specification", "Name": "Specification" } ] } } ] }

Fluorescein-12-dUTP

1 mM solution

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