For further processing only. Others Fluorescein-12-dUTP 1 mM solution Fluorescein-12-dUTP 3.6.13.2.1.1 1 mM solution 11375601103 Fluorescein-12-dUTP Fluorescein-12-dUTP Reagents, kits custom fill Not Available CustomBiotech product. Please contact your local representative. Will be supplied as ''Fluorescein-12-dUTP''. Unit of measure is ''nmol''. For other than the listed concentrations please inquire. 134344-32-4 en Enzymatic nonradioactive labeling reagent for cDNA synthesis, PCR, random primed labeling, nick-translation or primer extension. en Use Fluorescein-12-dUTP as a substrate for:Terminal TransferaseDNA polymerase I (holoenzyme and Klenow fragment)Taq DNA polymeraseReverse transcriptase (e.g., from AMV and M-MuLV)Fluorescein-12-dUTP replaces dTTP in the random-primed DNA labeling reaction or in nick translation reactions, as well as in PCR. The nucleotide also serves as a substrate for Terminal Transferase in 3'-end labeling.Fluorescein-labeled probes can be used for in situ hybridization with direct fluorescence detection and detection by ELISA using Anti-Fluorescein-AP, Fab fragments.Repeated fluorescence labeling using Tetramethylrhodamine-6-dUTP (red) and AMCA-6-dUTP (bright blue) is possible. en Appearance: Clear, yellow solution Fluorescein-12-dUTP (1 μmol ≙ 63.3 A495 units, phosphate buffer, 0.1 mol/L, pH 9.0): 1.0-1.1 mmol/L Purity (HPLC, including isomere): 85.0-100.0 area% Function test using In Situ Cell Death Detection Kit, Fluorescein (in situ hybridization): Corresponds to reference Stability: At -15 to -25°C within specification range for 12 months. Store dry. Protect from light. en