{ "ProductData": { "RelatedLinks": "", "RegulatoryDisclaimer1": "For further processing only.", "RegulatoryDisclaimer2": "", "StainLocalization": [ "" ], "DisclaimerGroup2": null, "ProductNameGlobal": "Fluorescein-12-dUTP 1 mM solution", "DisclaimerGroup1": "Other - Non IVD (DG_02)", "ControlTissue": [ "" ], "BrandName": "Fluorescein-12-dUTP", "LicenseDisclaimers": [ ], "Isotypes": "", "RegulatoryDisclaimer3": "", "ID": "3.6.13.2.1.1", "ProductNameAddition": "1 mM solution", "Species": [ "" ], "Clone": "" }, "ProductSpec": { "ProductSpecVariant": { "Chapters": [ { "Language": "English", "Value": "Nomenclature: Fluorescein-12-deoxyuridine-5'-triphosphate
Formula: C₃₉H₃₇N₄O₂₁P₃Li₄
Molecular weight: 1018.4 D", "Name": "CB - Properties" }, { "Language": "English", "Value": "Enzymatic nonradioactive labeling reagent for cDNA synthesis, PCR, random primed labeling, nick-translation or primer extension.", "Name": "Positioning" }, { "Language": "English", "Value": "Appearance: Clear, yellow solution
Fluorescein-12-dUTP (1 μmol ≙ 63.3 A₄₉₅ units, phosphate buffer, 0.1 mol/L, pH 9.0): 1.0-1.1 mmol/L
Purity (HPLC, including isomere): 85.0-100.0 area%
Function test using In Situ Cell Death Detection Kit, Fluorescein (in situ hybridization): Corresponds to reference
Stability: At -15 to -25°C within specification range for 12 months. Store dry. Protect from light.", "Name": "CB - Specification" }, { "Language": "English", "Value": "Use Fluorescein-12-dUTP as a substrate for:

• Terminal Transferase
• DNA polymerase I (holoenzyme and Klenow fragment)
• Taq DNA polymerase
• Reverse transcriptase (e.g., from AMV and M-MuLV)

Fluorescein-12-dUTP replaces dTTP in the random-primed DNA labeling reaction or in nick translation reactions, as well as in PCR. The nucleotide also serves as a substrate for Terminal Transferase in 3'-end labeling.
Fluorescein-labeled probes can be used for in situ hybridization with direct fluorescence detection and detection by ELISA using Anti-Fluorescein-AP, Fab fragments.
Repeated fluorescence labeling using Tetramethylrhodamine-6-dUTP (red) and AMCA-6-dUTP (bright blue) is possible.", "Name": "Applications" }, { "Language": "English", "Value": "Will be supplied as ''Fluorescein-12-dUTP''. Unit of measure is ''nmol''.
For other than the listed concentrations please inquire.", "Name": "CB - Order Information" }, { "Language": "English", "Value": "Nomenclature: Fluorescein-12-deoxyuridine-5'-triphosphate
Formula: C₃₉H₃₇N₄O₂₁P₃Li₄
Molecular weight: 1018.4 D", "Name": "Properties" }, { "Language": "English", "Value": "Appearance: Clear, yellow solution
Fluorescein-12-dUTP (1 μmol ≙ 63.3 A₄₉₅ units, phosphate buffer, 0.1 mol/L, pH 9.0): 1.0-1.1 mmol/L
Purity (HPLC, including isomere): 85.0-100.0 area%
Function test using In Situ Cell Death Detection Kit, Fluorescein (in situ hybridization): Corresponds to reference
Stability: At -15 to -25°C within specification range for 12 months. Store dry. Protect from light.", "Name": "Specification" } ] } }, "Product2Taxomony": { "Product2TaxomonyReferences": [ { "StructureSystemName": "OWP Product Solutions", "NodeName": "CustomBiotech", "StructureSystemID": null, "NodeID": "520" }, { "StructureSystemName": "Product Grouping", "NodeName": "CustomBiotech->Molecular Additional Applications", "StructureSystemID": null, "NodeID": "02-0073" }, { "StructureSystemName": "OWP Product Types", "NodeName": "Raw Materials and Reagents->Labeled Nucleotides", "StructureSystemID": null, "NodeID": "70-030-00" }, { "StructureSystemName": "Product Grouping", "NodeName": "CustomBiotech->Molecular Raw Materials", "StructureSystemID": null, "NodeID": "02-0136" }, { "StructureSystemName": "OWP Organization", "NodeName": "Roche Diagnostics Solution (RDS)->Customer Areas->Core lab->CustomBiotech and LifeScience", "StructureSystemID": null, "NodeID": "01-01-01-07-00" }, { "StructureSystemName": "OWP Techniques", "NodeName": "RT-PCR/qRT-PCR", "StructureSystemID": null, "NodeID": "300-00" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "11375601103", "MaterialDescription": "Fluorescein-12-dUTP", "RegisteredProductName": "", "GTIN": "", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "custom fill", "PackSizeDescPIM360": "", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as ''Fluorescein-12-dUTP''. Unit of measure is ''nmol''.
For other than the listed concentrations please inquire." } ] }, "Product2Products": { "Product2ProductReference": [ ] } }