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The neonatal Fc receptor (FcRn) immobilized in this chromatography column has a specific binding affinity to IgG. The strength and the pH dependency of the binding is different for structural variants of the IgG, and in this way the FcRn chromatography mimics the principle of the in vivo regulation of the antibody clearance from the blood stream. That means, for a specific IgG molecule a later elution in the chromatography with a rising pH predicts a longer half-life in vivo. And even small variations of the IgG structure cause a detectable shift of the elution point in the chromatogram, therefore, different structures can be differentiated, e.g. oxidized or aggregated variants, conjugates, Fc mutations, different Fab fragments.

Furthermore, FcRn has an additional binding site for albumin, and the column can also be used to characterize albumin preparations, e. g. for differentiation of oxidized, aggregated or conjugated variants thereof.", "Language": "en", "Country": "XG", "Code": "Applications" }, { "Name": "Benefits", "Value": "FcRn affinity chromatography provides information about pharmacokinetical characteristics of IgG variants in an easy and standardized way, replacing laborious methods like surface plasmon resonance spectroscopy or manual ELISA testing (reference: T. Schlothauer et. al., mAbs 5:4, 576-586, 2013).", "Language": "en", "Country": "XG", "Code": "Benefits" }, { "Name": "Specification", "Value": "Prepacked chromatography column supplied with standard fittings for liquid chromatography systems (FPLC, HPLC).
Matrix: FcRn protein bound to sepharose matrix
Volume: Approximately 1 ml resin bed volume
Binding capacity: ≥ 100 µg IgG
Reusability: Stable for ≥ 100 injections if used as recommended in the instructions
Maximal system pressure: 5 bar
Maximal flow rate: 0.5 mL/min", "Language": "en", "Country": "XG", "Code": "Specification" }, { "Name": "Content", "Value": "

Label	Function / Description	Content
FcRn Affinity Column Gen2	Prepacked chromatography column with 1 mL bed (matrix) volume of FcRn-Biotin-Streptavidin-Sepharose resin. Preassembled with the optimal fittings and filters applicable on all standard liquid chromatography systems, such as FPLC or HPLC. The resin is supplied in storage buffer: 16 mM MES-Na, 4 mM Tris-HCl, 140 mM NaCl, 20 Tablets/L Complete, 0.05% ProClin-300, pH 6.0. Ready-to-use and reusable. There may be a light white line in the middle of the column. This is not a weakness of the material. The mark is commonly generated in the manufacturing process and does not influence the stability.	1 column

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