For use in quality control/manufacturing process only. Others FcRn Affinity Column Gen2 FcRn Affinity Column Gen2 PID00000006 prepacked column for affinity chromatography 09 430 857 001 9 430 857 001 09430857001 9430857001 09430857001 FcRn Affinity Column Gen2 FcRn Affinity Column Gen2 07613336220832 Reagents, kits 1 column Not Available CustomBiotech product. Please contact your local representative. Unit of measure is "piece". The FcRn Affinity Column Gen2 enables fast and convenient analysis of monoclonal IgG antibodies with regard to their structural integrity and for prediction of their half-life in vivo as a therapeutic. en The prepacked column containing the FcRn matrix is ready for use on common liquid chromatography systems for the chromatographic differentiation of structural variants of IgG. en FcRn affinity chromatography provides information about pharmacokinetical characteristics of IgG variants in an easy and standardized way, replacing laborious methods like surface plasmon resonance spectroscopy or manual ELISA testing (reference: T. Schlothauer et. al., mAbs 5:4, 576-586, 2013). en Prepacked chromatography column supplied with standard fittings for liquid chromatography systems (FPLC, HPLC).Matrix: FcRn protein bound to sepharose matrixVolume: Approximately 1 ml resin bed volumeBinding capacity: ≥ 100 µg IgGReusability: Stable for ≥ 100 injections if used as recommended in the instructionsMaximal system pressure: 20 bar (290 psi)Maximal flow rate: 0.5 mL/min en The column is designed for chromatographic differentiation of structural variants of monoclonal IgG. FcRn analysis can provide insights into the structural and functional integrity of therapeutic immunoglobulins and enables improved conclusions on the half-life of the immunoglobulins in vivo:The neonatal Fc receptor (FcRn) immobilized in this chromatography column has a specific binding affinity to IgG molecules. The strength of the binding depends on structural variations of the IgG, like different Fab fragments, mutations in the Fc part, antibody isotypes, oxidized forms, aggregates, immune complexes, or antibody-drug conjugates. The FcRn affinity chromatography enables differentiation of such variants by peak patterns and retention times in the elution profile.Furthermore, the FcRn receptor has a binding affinity also to serum albumin, and the FcRn affinity chromatography can also be used to characterize albumin preparations, for example, for differentiation of oxidized, aggregated, or conjugated variants.Proteins binding to FcRn:Human IgG1, IgG2, IgG3, IgG4Some IgG of non-human species are able to bind human FcRnHuman albumin (often used as a fusion protein for therapeutic proteins) en

Accelerate antibody half-life analysis for faster lead candidate selection

The FcRn Affinity Column Gen2 is a prepacked chromatography tool designed for the rapid analysis of IgG antibody half-life and structural integrity. The column accelerates and standardizes the critical analysis of antibody half-life, helping you identify and advance promising lead candidates more efficiently.

Designed for seamless integration, this easy-to-use column works directly with your existing HPLC system, enabling automated processing and highly reproducible results.

rdoe_persona_userprofile

rdoe_persona_select_up

FcRn Affinity Column Gen2

Accelerate antibody half-life analysis for faster lead candidate selection

How does FcRn Affilinity Column Gen2 support enhanced analysis of antibody half-life?

Gain deep insights at multiple pH values

Ensure truly standardized analyses

Automate antibody processing

Which antibody properties can be distinguished and characterized with the FcRn Affinity Column Gen2?

Ordering information

Technical documents

Contact us

rdoe_persona_userprofile

rdoe_persona_select_up

FcRn Affinity Column Gen2

Accelerate antibody half-life analysis for faster lead candidate selection

How does FcRn Affilinity Column Gen2 support enhanced analysis of antibody half-life?

Gain deep insights at multiple pH values

Ensure truly standardized analyses

Automate antibody processing

Which antibody properties can be distinguished and characterized with the FcRn Affinity Column Gen2?

Related pages

Downloads

Ordering information

Technical documents

Contact us