For further processing only. Others FastStart Taq DNA Polymerase GMP Grade 5 U uL FastStart Taq DNA Polymerase, GMP Grade, 5 U/μl 3.6.17.2.9.4 from Thermus aquaticus BM, expressed in E. coli, solution 04659163103 FastStart Taq DNA Pol. GMP Grd, 5KU FastStart Taq DNA Polymerase, GMP Grade, 5 U/uL 07613336131695 Reagents, kits 5 kU Not Available CustomBiotech product. Please contact your local representative. Will be supplied as "FastStart Taq DNA Pol. Ind. GMP Grd, 5KU". Unit of measure is "piece". The enzyme is supplied without reaction buffer. EC 2.7.7.7 en Use FastStart Taq DNA Polymerase, GMP Grade, 5 U/μl, for:Hot start PCR and RT-PCR with high specificity, sensitivity and yieldSpecific amplification of DNA fragments from various sources of DNA and for diverse down-stream applicationsLabeling of DNA with modified nucleotides (e.g., DIG-dUTP, biotin-dUTP, fluorescein-dUTP)The prevention of carryover contamination between PCR reactions in combination with dUTP and Uracil-DNA GlycosylaseManufacture of amplification mixtures for regulated applications (e.g., in vitro diagnostics, quality control) with requests for more stringent validation en Achieve high specificity, sensitivity, and yield. Prevent the extension of non-specifically bound primers using this hot start enzyme. Obtain reliable results. Rely on the robust reaction performance, and high lot-to-lot consistncy of this product, thoroughly tested for a reproducible quality. Manufacturing and documentation are according to GMP (Good Manufacturing Practice) regulations. en Hot start Taq DNA Polymerase for highly specific and sensitive amplification using PCR. en Appearance: Clear to slightly opalescent, colorless solution Storage buffer: Tris/HCl, 20 mmol/L; KCl, 100 mmol/L; DTT, 1 mmol/L; EDTA, 0.1 mmol/L; Tween 20, 0.2% (v/v); glycerol, 50% (v/v); pH 9.0 at +25°C Volume activity: ≥5 U/μL Unit definition: One unit Taq DNA Polymerase is defined as the amount of heat-activated enzyme that incorporates 10 nmol of total deoxyribonucleosidetriphosphates into acid precipitable DNA within 30 minutes at +75°C under standard assay conditions. Unspecific endonucleases (λDNA): Not detectable in up to 25 U after 16 hours incubation at +37°C. Nicking activity (pBR322 DNA): Not detectable in up to 25 U after 16 hours incubation at +37°C. Ribonucleases (MS2 RNA): Not detectable in up to 25 U after 1 hour incubation at +37°C. Function test in PCR (50 pg human genomic DNA, 365 bp tPA fragment): Corresponds to reference (human genomic DNA, 284 bp ApoE fragment): Corresponds to reference Function test in qPCR using the LightCycler® System (human genomic DNA, β-globin gene): Corresponds to reference (plasmid DNA, β-globin gene): Corresponds to reference (reverse transcribed cDNA, PBGD gene): Corresponds to reference Bioburden: ≤50 CFU/mL Animal-derived additives: None Stability: At -15 to -25°C within specification range for 12 months. en