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  • Hot start PCR and RT-PCR with high specificity, sensitivity and yield
  • Specific amplification of DNA fragments from various sources of DNA and for diverse down-stream applications
  • Labeling of DNA with modified nucleotides (e.g., DIG-dUTP, biotin-dUTP, fluorescein-dUTP)
  • The prevention of carryover contamination between PCR reactions in combination with dUTP and Uracil-DNA Glycosylase
  • Manufacture of amplification mixtures for regulated applications (e.g., in vitro diagnostics, quality control) with requests for more stringent validation
", "Name": "Applications" }, { "Language": "en", "Value": "FastStart Taq DNA Polymerase is a chemically inactivated form of recombinant Taq DNA Polymerase. It remains inactive at temperatures up to +75°C. At higher temperatures, the modification is cleaved off and the polymerase acquires its enzymatic activity. Using FastStart Taq DNA Polymerase, PCR setup can be done conveniently at ambient temperature with no risk of nonspecific priming. The polymerase will not be activated until the initial denaturation step of the PCR protocol, at which point nonspecific hybridization can no longer occur.", "Name": "Background Information" }, { "Language": "en", "Value": "Achieve high specificity, sensitivity, and yield.
Prevent the extension of non-specifically bound primers using this hot start enzyme.", "Name": "Benefits" }, { "Language": "en", "Value": "Will be supplied as ''Fast Start Taq DNA Polymerase''. Unit of measure is ''kU''.
The enzyme is supplied without reaction buffer.", "Name": "CB - Order Information" }, { "Language": "en", "Value": "Appearance: Clear to slightly opalescent, colorless solution
Storage buffer: Tris/HCl, 20 mmol/L; KCl, 100 mmol/L; DTT, 1 mmol/L; EDTA, 0.1 mmol/L; Tween 20; 0.2% (v/v); glycerol, 50% (v/v); pH approximately 9.0 at +25°C
Volume activity: ≥5 U/μL
Unit definition: One unit Taq DNA Polymerase is defined as the amount of heat-activated enzyme that incorporates 10 nmol of total deoxyribonucleosidetriphosphates into acid precipitable DNA within 30 minutes at +75°C under standard assay conditions.
Unspecific endonucleases (λDNA): Not detectable in up to 25 U after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 25 U after 16 hours incubation at +37°C.
Ribonucleases (MS2 RNA): Not detectable in up to 25 U after 1 hour incubation at +37°C.
Function test in PCR
(50 pg human genomic DNA, 365 bp tPA fragment): Corresponds to reference
(200 ng human genomic DNA, 284 bp ApoE fragment): Corresponds to reference
Animal-derived additives: None
Stability: At -15 to -25°C within specification range for 18 months.", "Name": "Specification" }, { "Language": "en", "Value": "FastStart Taq DNA Polymerase is designed for hot start PCR and has to be heat-activated in the beginning of the reaction protocol.
Enzyme acivities: Highly processive 5'-3' DNA polymerase; double-strand specific 5'-3' exonuclease; no 3'-5' exonuclease activity
Heat activation: +95°C for 3-10 minutes (assay-dependent; recommendation is 10 minutes for full activation)
pH optimum: Approximately 9.0 (+25°C)
Temperature optimum: Approximately +75°C
Half life at +95°C: Approximately 40 minutes
Substrates: Incorporates dNTP, dUPT, dITP, various labeled or modified nucleotides (200 μmol/L each is recommended of normal dNTP, increased concentrations of variants).
Divalent ion requirement: Mg2+ (1.5 mmol/L standard concentration)", "Name": "Properties" } ] } } ] }

FastStart Taq DNA Polymerase, 5 U/μl

from Thermus aquaticus BM, expressed in E. coli, solution

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