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- Setup of PCR master mixtures, when highly concentrated components are requested
- Preparation of stabilized dried-down formulations of reaction mixtures
For further applications, see
FastStart Taq DNA Polymerase, GMP Grade, 5 U/μl",
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Appearance: Clear, colorless solution
Storage buffer: Tris/HCl, 20 mmol/L; KCl, 100 mmol/L; DTT, 1 mmol/L; EDTA, 0.1 mmol/L; Tween 20, 0.2% (v/v); glycerol, 50% (v/v); pH 9.0 at +25°C ±0.1
Volume activity: ≥100 U/μL
Unit definition: One unit Taq DNA Polymerase is defined as the amount of heat-activated enzyme that incorporates 10 nmol of total deoxyribonucleosidetriphosphates into acid precipitable DNA within 30 minutes at +75°C under standard assay conditions.
Unspecific endonucleases (λDNA): Not detectable in up to 25 U after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 25 U after 16 hours incubation at +37°C.
Ribonucleases (MS2 RNA): Not detectable in up to 25 U after 1 hour incubation at +37°C.
Exonucleases (
3H-DNA): Not detectable in up to 15 U after 4 hours incubation at +65°C.
Function test in PCR (50 pg human genomic DNA, 365 bp tPA fragment): Corresponds to reference
(human genomic DNA, 284 bp ApoE fragment): Corresponds to reference
Animal-derived additives: None
Stability: At -15 to -25°C within specification range for 12 months.",
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