{ "ProductData": { "RelatedLinks": "", "RegulatoryDisclaimer1": "For further processing only.", "RegulatoryDisclaimer2": "", "StainLocalization": [ "" ], "ProductType": "Others", "DisclaimerGroup2": null, "ProductNameGlobal": "FastStart Taq DNA Polymerase 100 U uL", "DisclaimerGroup1": "Non-IVD", "ControlTissue": [ "" ], "BrandName": "FastStart Taq DNA Polymerase, 100 U/μl", "LicenseDisclaimers": [ ], "Isotypes": "", "RegulatoryDisclaimer3": "", "ID": "3.6.17.2.9.3", "ProductNameAddition": "from Thermus aquaticus BM, expressed in E. coli, solution", "Species": [ "" ], "Clone": "" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/CB_IMAGE.png", "ImageType": "Image main" }, "Product2Taxomony": { "Product2TaxomonyReferences": [ { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "NodeName": "FastStart", "NodeID": "170" }, { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "NodeName": "DNA Polymerases", "NodeID": "70-042-00" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "Molecular Raw Materials", "NodeID": "02-0136" }, { "StructureSystemIdentifier": "OWP_Organization", "StructureSystemName": "Organization", "NodeName": "CustomBiotech and LifeScience", "NodeID": "01-01-01-07-00" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "Enzymes", "NodeID": "02-0014" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "NodeName": "PCR/qPCR", "NodeID": "210-00" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "NodeName": "CustomBiotech", "NodeID": "520" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeName": "Manufacturing", "NodeID": "180-00" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "04433785103", "MaterialDescription": "FastStart Taq DNA Pol. 100 U/µl", "RegisteredProductName": "FastStart Taq DNA Polymerase, 100 U/uL", "GTIN": "", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "custom fill", "PackSizeDescPIM360": "", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"FastStart Taq DNA Pol. 100 U/μl\". Unit of measure is \"kU\".
The enzyme is supplied without reaction buffer." } ] }, "Product2Products": { "Product2ProductReference": [ { "ProductID": "3.6.17.2.9.4", "BrandName": "FastStart Taq DNA Polymerase, GMP Grade, 5 U/μl", "ProductNameAddition": "from Thermus aquaticus BM, expressed in E. coli, solution", "ReferenceType": "Reagent", "Classification": [ { "IdentifierofStructureSystem": "OWP_Family", "NameofStructureSystem": "Product Families", "StructureNodeID": "170", "StructureGroupPath": "FastStart", "StructureGroupName": "FastStart" }, { "IdentifierofStructureSystem": "OWP_Product_Types", "NameofStructureSystem": "Product Types", "StructureNodeID": "70-042-00", "StructureGroupPath": "Raw Materials->DNA Polymerases", "StructureGroupName": "DNA Polymerases" }, { "IdentifierofStructureSystem": "Product_Grouping", "NameofStructureSystem": "Product Grouping", "StructureNodeID": "02-0136", "StructureGroupPath": "CustomBiotech->Molecular Raw Materials", "StructureGroupName": "Molecular Raw Materials" }, { "IdentifierofStructureSystem": "OWP_Organization", "NameofStructureSystem": "Organization", "StructureNodeID": "01-01-01-07-00", "StructureGroupPath": "Roche Diagnostics Solution (RDS)->Customer Areas->Core lab->CustomBiotech and LifeScience", "StructureGroupName": "CustomBiotech and LifeScience" }, { "IdentifierofStructureSystem": "Product_Grouping", "NameofStructureSystem": "Product Grouping", "StructureNodeID": "02-0014", "StructureGroupPath": "CustomBiotech->Enzymes", "StructureGroupName": "Enzymes" }, { "IdentifierofStructureSystem": "OWP_Techniques", "NameofStructureSystem": "Techniques", "StructureNodeID": "210-00", "StructureGroupPath": "PCR/qPCR", "StructureGroupName": "PCR/qPCR" }, { "IdentifierofStructureSystem": "Product_Solutions", "NameofStructureSystem": "Product Solutions", "StructureNodeID": "520", "StructureGroupPath": "CustomBiotech", "StructureGroupName": "CustomBiotech" }, { "IdentifierofStructureSystem": "Lab_Type", "NameofStructureSystem": "Lab Types", "StructureNodeID": "180-00", "StructureGroupPath": "Manufacturing", "StructureGroupName": "Manufacturing" }, { "IdentifierofStructureSystem": "Applications", "NameofStructureSystem": "Applications", "StructureNodeID": "99-00-00", "StructureGroupPath": "NOT APPLICABLE", "StructureGroupName": "NOT APPLICABLE" }, { "IdentifierofStructureSystem": "Disease_Areas", "NameofStructureSystem": "Disease Areas", "StructureNodeID": "99-00-00", "StructureGroupPath": "NOT APPLICABLE", "StructureGroupName": "NOT APPLICABLE" }, { "IdentifierofStructureSystem": "Pathogens", "NameofStructureSystem": "Pathogens", "StructureNodeID": "99-00-00", "StructureGroupPath": "NOT APPLICABLE", "StructureGroupName": "NOT APPLICABLE" }, { "IdentifierofStructureSystem": "Health_Topics", "NameofStructureSystem": "Health Topics", "StructureNodeID": "99-00-00", "StructureGroupPath": "NOT APPLICABLE", "StructureGroupName": "NOT APPLICABLE" } ] } ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Language": "en", "Value": "Use FastStart Taq DNA Polymerase, 100 U/μl, especially for:
  • Setup of PCR master mixtures, when highly concentrated components are requested
  • Preparation of stabilized dried-down formulations of reaction mixtures

For further applications, see FastStart Taq DNA Polymerase, GMP Grade, 5 U/μl", "Name": "Applications" }, { "Language": "en", "Value": "Will be supplied as \"FastStart Taq DNA Pol. 100 U/μl\". Unit of measure is \"kU\".
The enzyme is supplied without reaction buffer.", "Name": "CB - Order Information" }, { "Language": "en", "Value": "Concentrated hotstart Taq DNA Polymerase for highly specific and sensitive amplification using PCR; suitable for preparation of dried amplification mixes.", "Name": "Positioning" }, { "Language": "en", "Value": "See FastStart Taq DNA Polymerase, GMP Grade, 5 U/μl", "Name": "Background Information" }, { "Language": "en", "Value": "
  • Achieve high specificity, sensitivity, and yield.
    Prevent the extension of non-specifically bound primers using this hot start enzyme.
", "Name": "Benefits" }, { "Language": "en", "Value": "Appearance: Clear, colorless solution
Storage buffer: Tris/HCl, 20 mmol/L; KCl, 100 mmol/L; DTT, 1 mmol/L; EDTA, 0.1 mmol/L; Tween 20, 0.2% (v/v); glycerol, 50% (v/v); pH 9.0 at +25°C ±0.1
Volume activity: ≥100 U/μL
Unit definition: One unit Taq DNA Polymerase is defined as the amount of heat-activated enzyme that incorporates 10 nmol of total deoxyribonucleosidetriphosphates into acid precipitable DNA within 30 minutes at +75°C under standard assay conditions.
Unspecific endonucleases (λDNA): Not detectable in up to 25 U after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 25 U after 16 hours incubation at +37°C.
Ribonucleases (MS2 RNA): Not detectable in up to 25 U after 1 hour incubation at +37°C.
Exonucleases (3H-DNA): Not detectable in up to 15 U after 4 hours incubation at +65°C.
Function test in PCR
(50 pg human genomic DNA, 365 bp tPA fragment): Corresponds to reference
(human genomic DNA, 284 bp ApoE fragment): Corresponds to reference
Animal-derived additives: None
Stability: At -15 to -25°C within specification range for 12 months.", "Name": "Specification" }, { "Language": "en", "Value": "See FastStart Taq DNA Polymerase, GMP Grade, 5 U/μl", "Name": "Properties" } ] } } ] }

FastStart Taq DNA Polymerase, 100 U/μl

from Thermus aquaticus BM, expressed in E. coli, solution

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