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Setup of PCR master mixtures, when highly concentrated components are requested
Preparation of stabilized dried-down formulations of reaction mixtures
For further applications, see FastStart Taq DNA Polymerase, GMP Grade, 5 U/μl",
"Country": "XG",
"Code": "Applications",
"Name": "Applications"
},
{
"Language": "en",
"Value": "Will be supplied as \"FastStart Taq DNA Pol. 100 U/μl\". Unit of measure is \"kU\". The enzyme is supplied without reaction buffer.",
"Country": "XG",
"Code": "CB - Order Information",
"Name": "CB - Order Information"
},
{
"Language": "en",
"Value": "Concentrated hotstart Taq DNA Polymerase for highly specific and sensitive amplification using PCR; suitable for preparation of dried amplification mixes.",
"Country": "XG",
"Code": "Positioning",
"Name": "Positioning"
},
{
"Language": "en",
"Value": "See FastStart Taq DNA Polymerase, GMP Grade, 5 U/μl",
"Country": "XG",
"Code": "Background Information",
"Name": "Background Information"
},
{
"Language": "en",
"Value": "
Achieve high specificity, sensitivity, and yield. Prevent the extension of non-specifically bound primers using this hot start enzyme.
",
"Country": "XG",
"Code": "Benefits",
"Name": "Benefits"
},
{
"Language": "en",
"Value": "Appearance: Clear, colorless solution Storage buffer: Tris/HCl, 20 mmol/L; KCl, 100 mmol/L; DTT, 1 mmol/L; EDTA, 0.1 mmol/L; Tween 20, 0.2% (v/v); glycerol, 50% (v/v); pH 9.0 at +25°C ±0.1 Volume activity: ≥100 U/μL Unit definition: One unit Taq DNA Polymerase is defined as the amount of heat-activated enzyme that incorporates 10 nmol of total deoxyribonucleosidetriphosphates into acid precipitable DNA within 30 minutes at +75°C under standard assay conditions. Unspecific endonucleases (λDNA): Not detectable in up to 25 U after 16 hours incubation at +37°C. Nicking activity (pBR322 DNA): Not detectable in up to 25 U after 16 hours incubation at +37°C. Ribonucleases (MS2 RNA): Not detectable in up to 25 U after 1 hour incubation at +37°C. Exonucleases (3H-DNA): Not detectable in up to 15 U after 4 hours incubation at +65°C. Function test in PCR (50 pg human genomic DNA, 365 bp tPA fragment): Corresponds to reference (human genomic DNA, 284 bp ApoE fragment): Corresponds to reference Animal-derived additives: None Stability: At -15 to -25°C within specification range for 12 months.",
"Country": "XG",
"Code": "Specification",
"Name": "Specification"
},
{
"Language": "en",
"Value": "See FastStart Taq DNA Polymerase, GMP Grade, 5 U/μl",
"Country": "XG",
"Code": "Properties",
"Name": "Properties"
}
]
}
}
]
}
FastStart Taq DNA Polymerase, 100 U/μl
from Thermus aquaticus BM, expressed in E. coli, solution
