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Contents: Tris/HCl, 500 mmol/L; (NH4)2SO4, 50 mmol/L; KCl, 100 mmol/L; pH approximately 8.3 at +25°C
Unspecific endonucleases (λDNA, MWM II DNA): Not detectable in up to 20 μL after 16 hours incubation at +37°C.
Nicking activitiy (pBR322 DNA): Not detectable in up to 20 μL after 16 hours incubation at +37°C.
Ribonucleases (MS2 RNA): Not detectable in up to 20 μL after 1 hour incubation at +37°C.
Function test in PCR:
10 ng human genomic DNA, 4.8 kb tPA fragment/ 1.8 kb EPO fragment: Corresponds to specification
50 pg human genomic DNA, 365 bp tPA fragment: Corresponds to specification
Stability: At -15 to -25°C within specification range for 12 months.", "Name": "Specification" } ] } } ] }

FastStart PCR Buffer

10x conc., without MgCl2

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