{ "ProductData": { "ID": "3.6.17.6.5.2", "ProductType": "Others", "BrandName": "FastStart PCR Buffer", "ProductNameAddition": "10x conc., without MgCl₂", "RegulatoryDisclaimer1": "For further processing only.", "DisclaimerGroup1": "", "RegulatoryDisclaimer2": null, "DisclaimerGroup2": null, "RegulatoryDisclaimer3": null, "SampleType": [ ], "LicenseDisclaimers": [ ], "RelatedLinks": "", "Clone": "", "ControlTissue": [ "" ], "ISOtypes": "", "Species": [ "" ], "StainLocalization": [ "" ], "ProductNameGlobal": "FastStart PCR Buffer without MgCl2" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/CB_IMAGE_.png", "ImageType": "Image main" }, "Product2Taxonomy": { "Product2TaxonomyReferences": [ { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "NodeID": "170", "StructureNodeStatus": "valid", "NodeName": "FastStart" }, { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "NodeID": "70-009-00", "StructureNodeStatus": "valid", "NodeName": "Buffers" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeID": "02-0136", "StructureNodeStatus": "valid", "NodeName": "Molecular Raw Materials" }, { "StructureSystemIdentifier": "OWP_Organization", "StructureSystemName": "Organization", "NodeID": "01-01-01-07-00", "StructureNodeStatus": "valid", "NodeName": "CustomBiotech and LifeScience" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "NodeID": "210-00", "StructureNodeStatus": "valid", "NodeName": "PCR/qPCR" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "NodeID": "520", "StructureNodeStatus": "valid", "NodeName": "CustomBiotech" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeID": "180-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeID": "99-00-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "NodeID": "99-00-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "NodeID": "99-00-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "NodeID": "99-00-00", "StructureNodeStatus": "invalid", "NodeName": "" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "05917166103", "MaterialDescription": "FastStart PCR Buffer w/o MgCl2, 1 ml", "RegisteredProductName": "FastStart PCR Buffer", "GTIN": "07613336131671", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "1 mL", "PackSizeDescPIM360": "Not Available", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"PCR buffer (10X) w/o MgCl2\". Unit of measure is \"mL\"." }, { "MaterialNum": "12161494103", "MaterialDescription": "PCR buffer (10X) w/o MgCl2", "RegisteredProductName": "FastStart PCR Buffer", "GTIN": "", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "custom fill", "PackSizeDescPIM360": "Not Available", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"PCR buffer (10X) w/o MgCl2\". Unit of measure is \"mL\"." } ] }, "Product2Products": { "Product2ProductReference": [ ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Name": "CB - Order Information", "Value": "Will be supplied as \"PCR buffer (10X) w/o MgCl2\". Unit of measure is \"mL\".", "Language": "en", "Country": "XG", "Code": "CB - Order Information" }, { "Name": "Applications", "Value": "Use this buffer together with FastStart Taq DNA Polymerase whenever the amplification of difficult target requires a specific MgCl₂ concentration.", "Language": "en", "Country": "XG", "Code": "Applications" }, { "Name": "Positioning", "Value": "Standard reaction buffer without MgCl₂ for optimization of the MgCl₂ concentration in PCR using FastStart Taq DNA Polymerase.", "Language": "en", "Country": "XG", "Code": "Positioning" }, { "Name": "Specification", "Value": "Appearance: Clear, colorless solution
Contents: Tris/HCl, 500 mmol/L; (NH₄)₂SO₄, 50 mmol/L; KCl, 100 mmol/L; pH approximately 8.3 at +25°C
Unspecific endonucleases (λDNA, MWM II DNA): Not detectable in up to 20 μL after 16 hours incubation at +37°C.
Nicking activitiy (pBR322 DNA): Not detectable in up to 20 μL after 16 hours incubation at +37°C.
Ribonucleases (MS2 RNA): Not detectable in up to 20 μL after 1 hour incubation at +37°C.
Function test in PCR:
10 ng human genomic DNA, 4.8 kb tPA fragment/ 1.8 kb EPO fragment: Corresponds to specification
50 pg human genomic DNA, 365 bp tPA fragment: Corresponds to specification
Stability: At -15 to -25°C within specification range for 12 months.", "Language": "en", "Country": "XG", "Code": "Specification" } ] } } ] }